8 results on '"Qiu, Yunjiang"'
Search Results
2. Additional file 1 of REViewer: haplotype-resolved visualization of read alignments in and around tandem repeats
- Author
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Dolzhenko, Egor, Weisburd, Ben, Ibañez, Kristina, Rajan-Babu, Indhu-Shree, Anyansi, Christine, Bennett, Mark F., Billingsley, Kimberley, Carroll, Ashley, Clamons, Samuel, Danzi, Matt C., Deshpande, Viraj, Ding, Jinhui, Fazal, Sarah, Halman, Andreas, Jadhav, Bharati, Qiu, Yunjiang, Richmond, Phillip A., Saunders, Christopher T., Scheffler, Konrad, van Vugt, Joke J. F. A., Zwamborn, Ramona R. A. J., Chong, Samuel S., Friedman, Jan M., Tucci, Arianna, Rehm, Heidi L., and Eberle, Michael A.
- Abstract
Additional file 1: Supplementary methods. Description of the concordance study dataset; Description of the wrapper script; Evaluation of manual review performance; Comparison with haplotype-resolved assemblies, Comparison with other visualization software; REViewer allele structure of FMR1 reference samples; Comparison of STR genotypes extracted from long-read genome assembly.
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- 2022
- Full Text
- View/download PDF
3. Additional file 5 of REViewer: haplotype-resolved visualization of read alignments in and around tandem repeats
- Author
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Dolzhenko, Egor, Weisburd, Ben, Ibañez, Kristina, Rajan-Babu, Indhu-Shree, Anyansi, Christine, Bennett, Mark F., Billingsley, Kimberley, Carroll, Ashley, Clamons, Samuel, Danzi, Matt C., Deshpande, Viraj, Ding, Jinhui, Fazal, Sarah, Halman, Andreas, Jadhav, Bharati, Qiu, Yunjiang, Richmond, Phillip A., Saunders, Christopher T., Scheffler, Konrad, van Vugt, Joke J. F. A., Zwamborn, Ramona R. A. J., Chong, Samuel S., Friedman, Jan M., Tucci, Arianna, Rehm, Heidi L., and Eberle, Michael A.
- Abstract
Additional file 5: Figure S2. Read pileups in a region surrounding DMPK repeat expansion generated by (A) JBrowse, (B) IGV, and (C) REViewer.
- Published
- 2022
- Full Text
- View/download PDF
4. Additional file 4 of REViewer: haplotype-resolved visualization of read alignments in and around tandem repeats
- Author
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Dolzhenko, Egor, Weisburd, Ben, Ibañez, Kristina, Rajan-Babu, Indhu-Shree, Anyansi, Christine, Bennett, Mark F., Billingsley, Kimberley, Carroll, Ashley, Clamons, Samuel, Danzi, Matt C., Deshpande, Viraj, Ding, Jinhui, Fazal, Sarah, Halman, Andreas, Jadhav, Bharati, Qiu, Yunjiang, Richmond, Phillip A., Saunders, Christopher T., Scheffler, Konrad, van Vugt, Joke J. F. A., Zwamborn, Ramona R. A. J., Chong, Samuel S., Friedman, Jan M., Tucci, Arianna, Rehm, Heidi L., and Eberle, Michael A.
- Abstract
Additional file 4: Figure S1. REViewer pileup plots for some NA12878 STRs: (A) ATXN10, (B) RFC1, (C) CNPB.
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- 2022
- Full Text
- View/download PDF
5. Single-cell chromatin accessibility identifies pancreatic islet cell type- and state-specific regulatory programs of diabetes risk
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Chiou, Joshua, Zeng, Chun, Cheng, Zhang, Han, Jee Yun, Schlichting, Michael, Miller, Michael, Mendez, Robert, Huang, Serina, Wang, Jinzhao, Sui, Yinghui, Deogaygay, Allison, Okino, Mei-Lin, Qiu, Yunjiang, Sun, Ying, Kudtarkar, Parul, Fang, Rongxin, Preissl, Sebastian, Sander, Maike, Gorkin, David U, and Gaulton, Kyle J
- Subjects
Blood Glucose ,Epigenomics ,Somatostatin-Secreting Cells ,1.1 Normal biological development and functioning ,Human Embryonic Stem Cells ,Medical and Health Sciences ,Genetic ,Underpinning research ,Insulin-Secreting Cells ,Diabetes Mellitus ,Genetics ,Humans ,2.1 Biological and endogenous factors ,Polymorphism ,Aetiology ,Metabolic and endocrine ,Gene Expression Profiling ,Human Genome ,Diabetes ,High-Throughput Nucleotide Sequencing ,Cell Differentiation ,Fasting ,Pancreatic Polypeptide-Secreting Cells ,Biological Sciences ,Stem Cell Research ,Chromatin ,Glucagon-Secreting Cells ,Multigene Family ,KCNQ1 Potassium Channel ,Single-Cell Analysis ,Type 2 ,Transcription Factors ,Genome-Wide Association Study ,Developmental Biology - Abstract
Single-nucleus assay for transposase-accessible chromatin using sequencing (snATAC-seq) creates new opportunities to dissect cell type-specific mechanisms of complex diseases. Since pancreatic islets are central to type 2 diabetes (T2D), we profiled 15,298 islet cells by using combinatorial barcoding snATAC-seq and identified 12 clusters, including multiple alpha, beta and delta cell states. We cataloged 228,873 accessible chromatin sites and identified transcription factors underlying lineage- and state-specific regulation. We observed state-specific enrichment of fasting glucose and T2D genome-wide association studies for beta cells and enrichment for other endocrine cell types. At T2D signals localized to islet-accessible chromatin, we prioritized variants with predicted regulatory function and co-accessibility with target genes. A causal T2D variant rs231361 at the KCNQ1 locus had predicted effects on a beta cell enhancer co-accessible with INS and genome editing in embryonic stem cell-derived beta cells affected INS levels. Together our findings demonstrate the power of single-cell epigenomics for interpreting complex disease genetics.
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- 2021
6. Systematic analysis of binding of transcription factors to noncoding variants
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Yan, Jian, Qiu, Yunjiang, Ribeiro Dos Santos, André M, Yin, Yimeng, Li, Yang E, Vinckier, Nick, Nariai, Naoki, Benaglio, Paola, Raman, Anugraha, Li, Xiaoyu, Fan, Shicai, Chiou, Joshua, Chen, Fulin, Frazer, Kelly A, Gaulton, Kyle J, Sander, Maike, Taipale, Jussi, and Ren, Bing
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Binding Sites ,Genome ,Support Vector Machine ,General Science & Technology ,SELEX Aptamer Technique ,Human Genome ,Single Nucleotide ,Ligands ,Genetics ,Humans ,Disease ,Generic health relevance ,Polymorphism ,Transcription Factors ,Protein Binding ,Human - Abstract
Many sequence variants have been linked to complex human traits and diseases1, but deciphering their biological functions remains challenging, as most of them reside in noncoding DNA. Here we have systematically assessed the binding of 270 human transcription factors to 95,886 noncoding variants in the human genome using an ultra-high-throughput multiplex protein-DNA binding assay, termed single-nucleotide polymorphism evaluation by systematic evolution of ligands by exponential enrichment (SNP-SELEX). The resulting 828 million measurements of transcription factor-DNA interactions enable estimation of the relative affinity of these transcription factors to each variant in vitro and evaluation of the current methods to predict the effects of noncoding variants on transcription factor binding. We show that the position weight matrices of most transcription factors lack sufficient predictive power, whereas the support vector machine combined with the gapped k-mer representation show much improved performance, when assessed on results from independent SNP-SELEX experiments involving a new set of 61,020 sequence variants. We report highly predictive models for 94 human transcription factors and demonstrate their utility in genome-wide association studies and understanding of the molecular pathways involved in diverse human traits and diseases.
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- 2021
7. Inferring time series chromatin states for promoter-enhancer pairs based on Hi-C data
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Miko, Henriette, Qiu, Yunjiang, Gaertner, Bjoern, Sander, Maike, and Ohler, Uwe
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Cancer Research ,lcsh:QH426-470 ,Enhancer Elements ,Bioinformatics ,lcsh:Biotechnology ,1.1 Normal biological development and functioning ,Medical and Health Sciences ,Chromosomes ,Promoter Regions ,Genetic ,Underpinning research ,Hi-C ,lcsh:TP248.13-248.65 ,Information and Computing Sciences ,Genetics ,ddc:610 ,Promoter Regions, Genetic ,Methodology Article ,Histone modifications ,Bayes Theorem ,Biological Sciences ,Chromatin immunoprecipitation ,Chromatin ,Gene regulation ,lcsh:Genetics ,Enhancer Elements, Genetic ,Differentiation ,610 Medizin und Gesundheit ,Enhancer - Abstract
Background Co-localized combinations of histone modifications (“chromatin states”) have been shown to correlate with promoter and enhancer activity. Changes in chromatin states over multiple time points (“chromatin state trajectories”) have previously been analyzed at promoter and enhancers separately. With the advent of time series Hi-C data it is now possible to connect promoters and enhancers and to analyze chromatin state trajectories at promoter-enhancer pairs. Results We present TimelessFlex, a framework for investigating chromatin state trajectories at promoters and enhancers and at promoter-enhancer pairs based on Hi-C information. TimelessFlex extends our previous approach Timeless, a Bayesian network for clustering multiple histone modification data sets at promoter and enhancer feature regions. We utilize time series ATAC-seq data measuring open chromatin to define promoters and enhancer candidates. We developed an expectation-maximization algorithm to assign promoters and enhancers to each other based on Hi-C interactions and jointly cluster their feature regions into paired chromatin state trajectories. We find jointly clustered promoter-enhancer pairs showing the same activation patterns on both sides but with a stronger trend at the enhancer side. While the promoter side remains accessible across the time series, the enhancer side becomes dynamically more open towards the gene activation time point. Promoter cluster patterns show strong correlations with gene expression signals, whereas Hi-C signals get only slightly stronger towards activation. The code of the framework is available at https://github.com/henriettemiko/TimelessFlex. Conclusions TimelessFlex clusters time series histone modifications at promoter-enhancer pairs based on Hi-C and it can identify distinct chromatin states at promoter and enhancer feature regions and their changes over time. National Institutes of Health http://dx.doi.org/10.13039/100000002 Larry L. Hillblom Foundation http://dx.doi.org/10.13039/100001167
- Published
- 2020
8. Dynamic reorganization of the genome shapes the recombination landscape in meiotic prophase
- Author
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Patel, Lucas, Kang, Rhea, Rosenberg, Scott C, Qiu, Yunjiang, Raviram, Ramya, Chee, Sora, Hu, Rong, Ren, Bing, Cole, Francesca, and Corbett, Kevin D
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Male ,Genome ,urogenital system ,Synaptonemal Complex ,DNA Breaks ,Biophysics ,Biological Sciences ,Inbred C57BL ,Medical and Health Sciences ,Chromosomes ,Chromatin ,Mice ,Chromosome Pairing ,Spermatocytes ,Chemical Sciences ,Animals ,Meiotic Prophase I ,Spermatogenesis ,Homologous Recombination ,Developmental Biology - Abstract
In meiotic prophase, chromosomes are organized into compacted loop arrays to promote homolog pairing and recombination. Here, we probe the architecture of the mouse spermatocyte genome in early and late meiotic prophase using chromosome conformation capture (Hi-C). Our data support the established loop array model of meiotic chromosomes, and infer loops averaging 0.8-1.0 megabase pairs (Mb) in early prophase and extending to 1.5-2.0 Mb in late prophase as chromosomes compact and homologs undergo synapsis. Topologically associating domains (TADs) are lost in meiotic prophase, suggesting that assembly of the meiotic chromosome axis alters the activity of chromosome-associated cohesin complexes. While TADs are lost, physically separated A and B compartments are maintained in meiotic prophase. Moreover, meiotic DNA breaks and interhomolog crossovers preferentially form in the gene-dense A compartment, revealing a role for chromatin organization in meiotic recombination. Finally, direct detection of interhomolog contacts genome-wide reveals the structural basis for homolog alignment and juxtaposition by the synaptonemal complex.
- Published
- 2019
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