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Although electroacupuncture (EA) stimulation is a widely used therapy for chronic pain and comorbid psychiatric disorders, its long-term effects on chronic neuropathic pain-induced depression and the underlying mechanisms remain elusive. In the present study, we found that EA stimulation was able to restore adult neurogenesis in the ventral dentate gyrus (DG), by both increasing neuronal differentiation and restoring the normal morphology of newborn dendrites, in mice with spared nerve injury surgery. By ablating the Nestin+ neural stem cells (NSCs) via diphtheria toxin fragment A expression, we further proved that neurogenesis in the ventral DG was crucial to the long-term, but not the immediate antidepressant effect of EA, nor was it associated with nociception. Furthermore, we found that the restoration of neurogenesis was dependent on Tet1-mediated epigenetic modification upon EA treatment. Tet1 could bind to the promoter of the Prox1 gene, thus catalyzing its demethylation and facilitating its expression, which finally contributed to the restoration of neurogenesis and amelioration of depression-like behaviors induced by chronic neuropathic pain. Thus, we conclude that EA stimulation restores inhibited Tet1 expression in hippocampal NSCs of mice with chronic neuropathic pain, and increased Tet1 expression ameliorates hypermethylation of Prox1 and restores normal adult neurogenesis in the ventral DG, which contributes to the long-term antidepressant effect of EA.

An electrically responsive SiO2/aniline photonic crystal was fabricated and proved to possess low working potential, wide color tuning range, and good stability and reversibility in response.

Hydrogen sulfide (H

The blooms of coccolithophores are usually terminated by their viruses. However, little has been documented on the interplay between the host with virus under different environmental conditions. We investigated the relationship of Emiliania huxleyi (BOF92) and its virus (EhV99B1) after the coccolithophorid had acclimated to different levels of salinity (S35: 35‰; S25: 25‰) and CO2 (AC: 400 μatm; HC: 1000 μatm) for about 15 generations. Our results showed that the virus infection decreased the growth of E. huxleyi along with decreased photochemical efficiency and photosynthetic carbon fixation under all the combinations of the treatments. Lowered salinity exacerbated the impacts of the viral infection on the growth and photosynthetic performance of E. huxley. The elevated pCO2 appeared to have alleviated the impacts of the viral infection on its photosynthetic performance with enhanced levels of Fv/Fm, by about 49% under S35 and by about 16% under S25, respectively. Nevertheless, the elevated pCO2 enhanced the virus burst size by about 18% under the high and by about 46% under the low levels of salinity, respectively. It is concluded that reduced salinity under the elevated pCO2 exacerbates the impact of the virus on E. huxleyi, leading to the highest burst sizes and the lowest photosynthetic carbon fixation, and the enhanced photochemical efficiency and increased levels of Chl a under the elevated pCO2 could have facilitated the energy supply for the virus multiplication in the infected cells of E. huxleyi.

Electrically responsive photonic crystals are promising materials for electrophoretic color displays with better brightness and color saturation. However, electric field must always be applied to maintain the specific colors, which brings concerns about the power consumption and signal stability and reversibility. Here, we show an electrically responsive photonic crystal with two stable states at 0 V, which are the colored state or the colorless state with ordered or disordered particle arrangement. The color state can be reversibly switched by applying a short-time electrical field, just like in the case of commercial electrophoretic ink. With optimized recipe and electric field, the photonic crystals encapsulated in the prototype display panel are proved to have potentials in high resolution, multi-color, and greyscale display, which lays down a firm basis for reflective displays with low power consumption and good visibility.

Background: To investigate the anticancer mechanisms of di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT) in human colon cancer cells. Human colorectal carcinoma (HCC) is one of the most commonly diagnosed cancers in both males and females. Current studies have found that iron chelators can be used as novel anticancer drugs; however, the anticancer activity of iron chelators and their target genes in HCC has been rarely reported. Methods: Dp44mT was used to treat two colorectal tumor cell lines, SW480 and HT-29. The proapoptotic effects of different concentrations of Dp44mt were measured using flow cytometry and Hoechst 33258 staining. Ferric ammonium citrate (FAC) was used as an additional iron donor to inhibit the effects of Dp44mT. Apoptosis and DNA damage-related proteins were examined by Western blot analysis. Results: In this study, we found that the iron chelators Dp44mT could induce the apoptosis in two colorectal tumor cell lines SW480 and HT-29, upregulate the expression level of p-histone H2A.X, and inhibit the phosphorylation level of mTOR in a dose-dependent way. Those effects could be reversed by the additional iron donor FAC. Conclusion: These data indicate that iron depletion and/or the presence of iron can modulate the HCC apoptosis progression in vitro, which may be a potential target for future HCC therapy. Keywords: Dp44mT, cell apoptosis, mTOR, DNA damage

Mediastinal cysts are uncommon, and their diagnosis remains a clinical challenge, especially for patients with a solid mass on computed tomography (CT). Endoscopic ultrasound (EUS) is considered a valuable method to differentiate mediastinal cysts and EUS-fine needle aspiration (FNA) is a strategy for obtaining specimens from the cysts for cytological diagnosis. This study aims to evaluate the safety and utility of EUS-FNA for diagnosis of mediastinal cysts.This was a retrospective analysis of patients who underwent EUS-FNA with 19-gauge needle at Tianjin Medical University Cancer Institute and Hospital and were further diagnosed with mediastinal cysts confirmed by cytological and surgical pathological results between January 2016 and December 2020. Safety was estimated by the incidence of reported adverse events (AEs). Patients were followed for 48 hours and 1 week after the EUS-FNA procedure to evaluate AEs.A total of 20 patients were diagnosed with mediastinal cysts using EUS-FNA, yet only 5 were diagnosed by CT. There were 15 patients diagnosed with bronchogenic cyst, 4 with enteric cyst, and 1 with pericardial cyst. The EUS appearance of cyst content varied, ranging from anechoic (4 cases) to hypoechoic (16 cases). AEs occurred in 2/20 (10%) patients after the EUS-FNA indicating an acceptable low rate of AEs. For all anechoic cysts that underwent complete FNA drainage, 3 patients had good prognosis, whereas 1 experienced recurrence. For 16 patients with hypoechoic cysts, adequate tissue was obtained for cytological examination. No patient developed an infection-related complication.For the diagnosis of mediastinal cysts, EUS-FNA was more accurate than CT. The EUS-FNA of mediastinal cysts is safe with an acceptable low rate of AEs when antibiotic prophylaxis is used postoperatively. Cysts containing free-flowing fluid can be achieved with complete needle drainage by a single pass with a 19-gauge needle.

In this study, the two-phase flow coordination characteristics between α and β phases of H62 brass made by up-drawing continuous casting are investigated based on the upsetting process. An in situ and new research method for two-phase flow is put forward, and the two-phase flow and grain refinement characteristics are observed under different deformation conditions. The results show that α phase flows fast under 400 °C, β phase is pulled and overridden by α phase under this temperature. When the temperature increases to 500 °C, which is higher than β phase transition temperature, the flow velocity of β phase increases, and the deformation of β phase is found to bulge. The flow of β phase is more sensitive to low deformation rates than α phase. The deformation amount has a more significant impact on β phase than α phase, and the deformation of β phase promotes the grain fragmentation and refinement of α phase accompanied by huge β phase bulging obviously. Under the conditions of high temperature, low deformation rate, and large deformation amount, both phase α and β of up-drawing continuous casting brass alloy are broken and the grains are refined. Based on the two-phase flow characteristic, numerical simulation is used to obtain the optimal continuous extrusion parameters of the H-shaped wire of up-drawing continuous casting H62 brass. Then, the optimized complex cross-section wire is prepared by continuous extrusion experiment. This research aims to provide guidance for the complex processing of two-phase alloys.

New strategies enhancing both targetability of chemotherapy drugs and synergistic effects of chemotherapy and immunotherapy remain a major challenge for the solid tumor therapy. In this research, a novel biomimetic nanoparticle system possessed function of tumor targeting and immune synergy was designed to accomplish the above requirements. The membranes was coated on mesoporous silica nanoparticles loaded with chemotherapy drugs named doxorubicin (DOX); and it was modified by the glycosyl-phosphatidylinositol (GPI) anchored anti-HER2 single chain variable fragment (scFv) and the GPI anchored co-stimulatory molecule CD80 to promote solid tumor targeted chemotherapy and cooperated immunotherapy, respectively. The effects of as-prepared nano therapeutic system in both tumor targeted chemotherapy and collaborated cellular immune response were investigated through in vitro and in vivo experiments. The results showed that the novel biomimetic therapeutic system can significantly promote antitumor efficiency in vitro and in vivo experiments. Besides, this therapeutic system further enhances antitumor capacity by increasing the CD8+ T-cell activation and the cytokine production and reducing the myeloid-derived suppressor cells (MDSCs) levels in tumors.

New strategies enhancing both cellular immune response and targetability of tumor immunomodulator remain a major challenge for the immunotherapy in the solid tumor. In this research, a novel biomimetic immuno-chemotherapy composite nanoparticle system was designed as a breast tumors targeted drug. The cell membranes were modified by the glycosyl-phosphatidylinositol (GPI) anchored anti-HER2 single chain variable fragment (scFv) and the GPI anchored co-stimulatory molecule CD80; and the membranes was coated on mesoporous silica nanoparticles loaded with Doxorubicin (DOX). The effects of as-prepared therapeutic system in initiating both tumor targetability and cellular immune response were investigated through in vitro and in vivo experiments. The results showed that therapeutic system greatly promoted the CD8+ T-cell activation and the cytokine production; and reduced the myeloid-derived suppressor cells (MDSCs) levels in tumors. This novel biomimetic therapeutic system can significantly promote immunomodulatory and antitumor activity in vitro and in vivo experiments.

Amorphous photonic crystals (APCs) assembled by mesoporous SiO2 particles were first used to develop thin layer chromatography (TLC) with high separation efficiency and convenient colorimetric recognition. The m-SiO2 APC film has a large number of micron-scale pores, which significantly accelerate the solvent diffusion and address the problem of the short-distance development. Meanwhile, the abundant mesopores in m-SiO2 particles intensify the adsorption–desorption between analytes and APC film. Although the critical micron-scale pores were introduced by the sacrifice of long-range ordered structure, the APC film still has uniform structural colors due to its short-range ordered structure. Therefore, the APC film retains the advantage of colorimetric recognition, where the loaded chemicals cause an increased refractive index and a structural-color change. Compared to traditional SiO2 gel TLC, the sample recognition in APC TLC was simplified because chemical compounds, which must be dyed, can be directly recognized now. Meanwhile, the separation resolution achieved by m-SiO2 APC TLC was also improved due to the enhancement of adsorption–desorption.

Traditional thermochromic photonic crystal (PC) usually has a slow and reversible thermal response, which limits its application in thermal printing. Here, the authors develop a thermochromic "SiO

Expensive instruments and complicated data processing are often required to discriminate solvents with similar structures and properties. Colorimetric sensors with high selectivity, low cost, and good portability are highly desirable to simplify such detection tasks. Herein, we report the fabrication of a photonic crystal sensor based on the self-assembled resorcinol formaldehyde (RF) hollow spheres to realize colorimetric sensing of polar solvents, including homologs and isomers based on the saturated diffusion time. The diffusion of solvent molecules through the photonic crystal film exhibits a unique three-step diffusion profile accompanied by a dynamic color change, as determined by the physicochemical properties of the solvent molecules and their interactions with the polymer shells, making it possible to accurately identify the solvent type based on the dynamic reflection spectra or visual perception. With its superior selectivity and sensitivity, this single-component colorimetric sensor represents a straightforward tool for convenient solvent detection and identification.

A novel experimental setup for the generation of arbitrary vector beams is proposed. The system major includes two reflective liquid crystal spatial light modulators (RLC-SLM) and a polarizing beam splitting prism. Moreover, this method is not limited by the wavelength of light wave and the pixel size of SLM. Theoretical analysis shows that when Gaussian beam or a plane beam is illuminated on a computer-generated hologram (CGH) specially designed in this work, the complex amplitudes of the vector field’s two orthogonal polarization components may be changed by modifying the encoding parameters, resulting in a vector beam with arbitrary complex amplitude and polarization in the output field. The experimental results also show that the two independent coding channels of the device have good polarization-selective imaging ability, which greatly improves the flexibility of generating arbitrary vector beams.

The mangrove wetland in Dongzhai Harbor, Hainan Province was taken as a research area for the collection of samples of sediments and other media. The Pd content was determined by microwave digestion and inductively coupled plasma mass spectrometry (ICP-MS), and the factors influencing the accumulation of the platinum group element (PGE) Pd in mangrove wetland sediments were discussed. The results showed that the average concentrations of Pd in the surface sediments, plants and water of mangrove wetlands were 4.81 ng/g (1.51~8.15 ng/g), 0.94 ng/g (ND ~ 4.34 ng/g), and 0.11 ng/mL (ND ~ 0.27 ng/mL), respectively. Pd was mainly concentrated in the sediments, which absorb most of the Pd in the mangrove wetland environment. From the wetland estuary to offshore, the sediment Pd showed a decreasing trend. The land origin of Pd in mangrove wetland was explained. Extending from the mudflat to the mangrove, the “V” shape becomes the main distribution pattern of sediment Pd, which is higher in the mudflat and forest, and lowest in the forest edge. In the vertical direction, the average concentrations of Pd were the highest at the surface (10–20 cm) and decreased with increasing depth. Pd concentrations in the sediment in the dry season was higher than that in the wet season. Pd was significantly correlated with pH and Eh. This study can help clarify the environmental geochemical process of PGEs in mangrove wetlands and has important scientific significance and practical value for the environmental protection and utilization of mangrove wetlands.

Macroalgae play a crucial role in coastal marine ecosystems, but they are also subject to multiple challenges due to tidal and seasonal alterations. In this work, we investigated the photosynthetic response of Pyropia yezoensis to ultraviolet radiation (PAR: 400-700 nm; PAB: 280-700 nm) under changing temperatures (5, 10 and 15°C) and light intensities (200, 500 and 800 μmol photons m-2 s-1 ). Under low light intensity (200 μmol photons m-2 s-1 ), P. yezoensis showed the lowest sensitivity to ultraviolet radiation, regardless of temperature. However, higher temperatures inhibited the repair rates (r) and damage rates (k) of photosystem II (PSII) in P. yezoensis. However, under higher light intensities (500 and 800 μmol photons m-2 s-1 ), P. yezoensis showed higher sensitivity to UV radiation. Both r and the ratio of repair rate to damage rate (r:k) were significantly inhibited in P. yezoensis by PAB, regardless of temperature. In addition, higher temperatures significantly decreased the relative UV-inhibition rates, while an increased carbon fixation rate was found. Our study suggested that higher light intensities enhanced the sensitivity to UV radiation, while higher temperatures could relieve the stress caused by high light intensity and UV radiation.

Traditional SiO2 gel-based thin-layer chromatography (TLC) is unable to separate substances with close structures, and its detection requires dyeing when the sample has neither fluorescence nor ultraviolet (UV) absorption signals. Here, high-performance TLC was developed based on metal-organic framework-coated photonic crystal (MOF/PC) films. Using the MIL-100/PC as the TLC plate, the cresol isomers can now be separated in a 2 cm development distance due to the enhanced adsorption-desorption and the good selectivity from the micropores. Meanwhile, the cresol changes the refractive index of MOF/PC, which produces color changes for sample recognition. Compared to the traditional TLC, the MOF/PC TLC shows much higher selectivity toward the analytes, which fully compensates its limited plate numbers and ensures a high-resolution separation. It also provides a convenient solution for chemical analysis as the detection based on structural color change does not require UV irradiation or dyeing treatment.

To optimize the farming strategy of economically important seaweeds, we grew Gracilaria lemaneiformis at different seawater depths under natural solar radiation with or without UV irradiances. Our results showed that solar UV radiation (UVR, 295–395 nm) significantly inhibited its growth and photosynthesis in the surface seawater; however, such a negative effects became inverse at 1.7 m with levels of UVR being about 11.7 W m− 2. The maximal growth rates were found at 1.7, 0.8 m when the thalli grown at PAR + UVR or PAR only conditions, respectively. UV-absorbing compounds in the algal thalli were up to 85% higher under PAR + UVR compared to PAR alone treatment and they increased with decreased depths even in the absence of UVR. The enhanced growth rate in the presence of UVR could be attributed to enhanced photosynthesis under moderate levels of solar radiation by UV-A. Our study clearly showed that UV-induced growth inhibition could be minimized or avoided by optimizing the alga's farming depth in different waters of different transparency, so that Gracilaria's biomass production could be increased.

To enhance biodiesel production and quality from a bloom-forming diatom Skeletonema costatum, a two-stage model, in which cells were cultured in nutrient replete conditions first and then transferred to nutrient limitation conditions, was explored. Compared to one-stage model, nutrient limitation in the second stage significantly increased lipid content in spite of decreasing growth; consequently, Si-limitation and N-Si-limitation respectively increased lipid productivity by 37.6% and 76.7% for 6 h induction, and 42.8% and 113.7% for 12 induction. Nutrient limitation enhanced the proportions of saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) but reduced polyunsaturated fatty acid (PUFA). Therefore, N-Si-limitation reduced iodine value by 33.7% and 45.6% but increased cetane number by 6.4% and 21.6% for 6 and 24 h induction, respectively. These findings indicate that the two-stage model with N-Si-limitation can enhance lipid productivity as well as biodiesel quality from diatoms.

An invisible photonic crystal (PC) pattern encrypted at the dry state and decrypted by solvent is a new anticounterfeiting material system, but its diversity and complexity in response are still required to be increased to enhance the anticounterfeiting effect. Herein, a triple‐state invisible pattern encrypted in hollow‐silica/polyurethane (h‐SiO2/PU) film with two decryption mechanisms is developed based on the asymmetric solvent response of h‐SiO2/PU with different SiO2 shell thicknesses. The pattern is printed via etching SiO2 shell in the designed region, which creates an etched “pattern” containing thin‐shell h‐SiO2 particles and an unetched “background” containing thick‐shell particles. Different from the traditional invisible PC patterns, the current system possesses triple states, including a “dry” invisible state composed of two close inverse opal structures, a “wet” invisible state composed of inverse opals filled with water, and an ethanol‐soaking visible state due to the prevention of solvent diffusion by the thicker shell. The invisible patterns are reversibly and randomly switched among these three states, which suggest that they are decrypted from the dry invisible state through selective solvent infiltration or from the wet invisible state through selective extraction. Such increased diversity and complexity make this invisible PC pattern ideal material for anticounterfeiting applications.

Rural to urban migration under China’s rapid urbanization has created inefficient use of rural homestead land. To address these issues, there is increasing interest in rural homestead system reform in China. Although several reforms have been implemented in pilot areas, reviews of the related accumulated literature are rare. The objectives of this study are the following: (i) to discuss the research transition from the “separation of two rights” to the “separation of three rights” in homestead management and (ii) draw conclusions on current research conditions, hot topics, and trends. This study provides a reference for further and deeper rural homestead system reform in China and regions worldwide. We collected and sorted 640 research papers on the topic of the separation of three rights in homestead (hereinafter referred to as STRH) or the homestead land use rights from China National Knowledge Infrastructure (CNKI) and used CiteSpace and VOSviewer to visualize and map knowledge domains. The results show that: (1) From the study of the homestead land use rights to the study of the STRH, there is pronounced synergy between national policies and the number of published papers. The STRH has become a popular research field since 2018. (2) Most researchers are experts and scholars engaged in the study of agricultural economics, civil and commercial law, administrative law, or local legal systems. (3) Current and future studies on the STRH are and will be conducted in terms of the definition of concepts and connotations, confirmation of rights and registration, implementation paths, rural homestead system reform, rural revitalization strategy, reform challenges and countermeasures, risk control, and overall planning. (4) The studies on the STRH have integrity, and show characteristics of inheritance between it and studies on the homestead land use rights. (5) Studies on typical cases for the STRH should be conducted in combination with the latest strategic requirements such as rural land system reform, rural revitalization strategy, and integrated urban and rural development.

This paper mainly studies the effect of shear thickening gel on the impact resistance of polyurethane foam. First, flexible polyurethane foams with strain rate sensitivity, shape memory, and impact resistance were prepared by a convenient one-step foaming method. Then, the surface morphology and impact resistance of the composites were characterized by SEM, AFM, and low-velocity impact tests. The results demonstrated that the addition of the shear thickening gel makes the cell size of the composites more uniform and the molecular chain arrangement more regular. When the amount of the shear thickening gel was 20 parts, the surface roughness of polyurethane foams was increased from 14.7 to 74.7 nm. More importantly, the addition of the shear thickening gel significantly enhanced the impact resistance of polyurethane foam. When the impact velocity was 5.5 m s−1, the ratio of absorbed energy to impact energy of the FPUF-15pbw reached 91.35%, which was 20.12% higher than the FPUF-0pbw.

Little empirical research is available on the evaluation of affective objectives under the New Curriculum Reform in China. This study investigated 1563 volunteers from three lower secondary school students in Gansu Province, China, to explore the factor structure of affective objectives and to assess students' affective attainments in the context of western rural China. The final affective objectives questionnaire was able to put forth a statistically valid three-factor model that provides solutions for nine affective objectives: bravery, self-confidence, independence, fairness, integrity, forgiveness, gratitude, love of learning and aesthetic. The affective objectives with the best performance in these rural students were gratitude, independence and integrity, while the three lowest rated were forgiveness, bravery and confidence. Male students rated themselves statistically higher on confidence, bravery and love of learning, whereas female students rated themselves statistically higher on independence, fairness and aesthetic. Comparing different grade groups revealed that seventh-grade students reported higher scores than eighth- and ninth-grade students for confidence, integrity, love of learning and aesthetic, whereas ninth-grade students rated slightly higher than eighth-grade students on bravery and gratitude.

This work reported a high quality photonic crystal film with an ultra-narrow photonic bandgap obtained via a chemical synthetic route. The bandgap is much narrower than that of traditional colloidal crystals, which makes the film qualified for use in optical devices. The narrow PBG originates from not only the high crystallinity and uniform orientations of microcrystals within the film but also the very close refractive indices between the silica and the polymer matrix. Due to the matching of the refractive index, the amorphous contents of the film are optically transparent and do not interfere with the reflection, so that the photonic crystal film is tolerant of the existence of disordered contents.

Nitrogen adsorption-desorption for mesopore characterization requires the using of expensive instrumentation, time-consuming processes, and the consumption of liquid nitrogen. Herein, a new method is developed to measure the pore parameters through mixing a mesoporous substance with a supersaturated SiO2 colloidal solution at different temperatures, and subsequent rapid measurement of reflection changes of the precipitated liquid photonic crystals. The pore volumes and diameters of mesoporous silica were measured according to the positive correlation between unit mass reflection change (Δλ/m) and pore volume (V), and the negative correlation between average absorption temperature (T) and pore diameter (D). This new approach may provide an alternative method for fast, convenient and economical characterization of mesoporous materials.

The effective document classification is a powerful technique to deal with the huge amount of e-government documents automatically instead of accomplishing them manually. The word-to-vector (word2vec) model, which converts semantic word into low-dimensional vectors, could be successfully employed to classify the e-government documents. In this paper, we propose the cooperative expressions of word vector (Co-word-vector), whose multi-granularity of integration explores the possibility of modeling documents in the semantic space. Meanwhile, we also aim to improve the weighted continuous bag of words model based on word2vec model and distributed representation of topic-words based on LDA model. Furthermore, combining the two levels of word representation, performance result shows that our proposed method on the e-government document classification outperform than the traditional method.

Invisible photonic prints shown by deformation are prepared by soaking the mechanochromic photonic paper with crosslinker (PEGDA) and subsequently crosslinking part of the paper through a photo lithography process. The key point of this new technique is creating patterns and background with very close photonic structures but different mechanochromic capabilities, so that the invisible photonic patterns in relaxed state can be revealed under deformation due to the nonuniform change in photonic structure. Based on the relationship between crosslinking level and the reflection changes during deformation, one can conclude that a low crosslinking level favors the hiding of invisible patterns and a high crosslinking level favors the showing of patterns. The as-prepared samples can instantly and reversibly show the patterns by deformation and hide them by relaxation for many times, and the encapsulation by PDMS rubber prolongs its life time and enhances its durability in practical usages. The current printing technique is capable of creating invisible photonic prints in both macroscale and microscale range, which makes them potentially useful for security and antifraud applications in daily life.

A SiO2 colloidal photonic crystal film with a hierarchical porous structure is fabricated to demonstrate an integrated separation and colorimetric detection of chemical species for the first time. This new photonic crystal based thin layer chromatography process requires no dyeing, developing and UV irradiation compared to the traditional TLC. The assembling of mesoporous SiO2 particles via a supersaturation-induced-precipitation process forms uniform and hierarchical photonic crystals with micron-scale cracks and mesopores, which accelerate the diffusion of developers and intensify the adsorption/desorption between the analytes and silica for efficient separation. Meanwhile, the chemical substances infiltrated to the voids of photonic crystals cause an increase of the refractive index and a large contrast of structural colors towards the unloaded part, so that the sample spots can be directly recognized with the naked eye before and after separation.

Red tide and green tide are two common algal blooms that frequently occur in many areas in the global oceans. The algae causing red tide and green tide often interact with each other in costal ecosystems. However, little is known on how future CO2-induced ocean acidification combined with temperature variation would affect the interaction of red and green tides. In this study, we cultured the red tide alga Skeletonema costatum and the green tide alga Ulva linza under ambient (400 ppm) and future CO2 (1000 ppm) levels and three temperatures (12, 18, 24 °C) in both monoculture and coculture systems. Coculture did not affect the growth rate of U. linza but significantly decreased it for S. costatum. Elevated CO2 relieved the inhibitory effect of U. linza on the growth of S. costatum, particularly for higher temperatures. At elevated CO2, higher temperature increased the growth rate of S. costatum but reduced it for U. linza. Coculture with U. linza reduced the net photosynthetic rate of S. costatum, which was relieved by elevated CO2. This pattern was also found in Chl a content, indicating that U. linza may inhibit growth of S. costatum via harming pigment synthesis and thus photosynthesis. In monoculture, higher temperature did not affect respiration rate of S. costatum but increased it in U. linza. Coculture did not affect respiration of U. linza but stimulated it for S. costatum, which was a signal of responding to biotic and/abiotic stress. The increased growth of S. costatum at higher temperature and decreased inhibition of U. linza on S. costatum at elevated CO2 suggest that red tides may have more advantages over green tides in future warmer and CO2-enriched oceans.

Traditional photonic sensing based on the change of balanced reflection of photonic structures can hardly distinguish chemical species with similar refractive indices. Here a sensing method based on the dynamic reflection spectra (DRS) of photonic crystal gel has been developed to distinguish even homologues, isomers and solvents with similar structures and physical properties. There are inherent relationships between solvent properties, diffusion behaviour and evolution of reflection signals, so that the geometric characteristics of DRS pattern including ascending/descending, colour changes, splitting/merging and curvature of reflection band can be utilized to recognize different organic solvents. With adequate solvents being tested, a database of DRS patterns can be established, which provide a standard to identify an unknown solvent., Photonic sensing is a method for detecting individual chemical species, but can fail when they are sufficiently similar in physical properties. Here, the authors report a method that can distinguish even very closely related species, such as homologues and chemical isomers.

Efficient and large scale printing of photonic crystal patterns with multicolor, multigrayscale, and fine resolution is highly desired due to its application in smart prints, sensors, and photonic devices. Here, an electric-field-assisted multicolor printing is reported based on electrically responsive and photocurable colloidal photonic crystal, which is prepared by supersaturation-induced self-assembly of SiO2 particles in the mixture of propylene carbonate (PC) and trimethylolpropane ethoxylate triacrylate (ETPTA). This colloidal crystal suspension, named as E-ink, has tunable structural color, controllable grayscale, and instantly fixable characteristics at the same time because the SiO2/ETPTA-PC photonic crystal has metastable and reversible assembly as well as polymerizable features. Lithographical printing with photomask and maskless pixel printing techniques are developed respectively to efficiently prepare multicolor and high-resolution photonic patterns using a single-component E-ink.