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12 results on '"Puri, Pier Lorenzo"'

1. Additional file 1 of Acute conversion of patient-derived Duchenne muscular dystrophy iPSC into myotubes reveals constitutive and inducible over-activation of TGFβ-dependent pro-fibrotic signaling

Author

Caputo, Luca, Granados, Alice, Lenzi, Jessica, Rosa, Alessandro, Ait-Si-Ali, Slimane, Puri, Pier Lorenzo, and Albini, Sonia

Abstract

Additional file 1: Figure S1. Myogenic potential of Control and DMD iPSC expressing inducible epB vectors for BAF60C and MyoD.

Published
2020
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2. Additional file 5 of Acute conversion of patient-derived Duchenne muscular dystrophy iPSC into myotubes reveals constitutive and inducible over-activation of TGFβ-dependent pro-fibrotic signaling

Author

Caputo, Luca, Granados, Alice, Lenzi, Jessica, Rosa, Alessandro, Ait-Si-Ali, Slimane, Puri, Pier Lorenzo, and Albini, Sonia

Subjects
musculoskeletal system
Abstract

Additional file 5: Figure S5. Pro-fibrotic gene activation in differentiated human myoblasts following electrical pacing.

Published
2020
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12. MyoD induces ARTD1 and nucleoplasmic poly-ADP-ribosylation during fibroblast to myoblast transdifferentiation

Author

Luca Caputo, Alessandra Dall’Agnese, Enrique Vidal, Chiara Nicoletti, Roni H. G. Wright, Ann-Katrin Hopp, Michael O. Hottiger, Lavinia Bisceglie, Miguel Beato, Kapila Gunasekera, Pier Lorenzo Puri, François Le Dily, Bisceglie, Lavinia, Hopp, Ann-Katrin, Gunasekera, Kapila, Wright, Roni H.G., Le Dily, François, Vidal, Enrique, Dall'Agnese, Alessandra, Caputo, Luca, Nicoletti, Chiara, Puri, Pier Lorenzo, Beato, Miguel, Hottiger, Michael O., University of Zurich, and Hottiger, Michael O

Subjects
0301 basic medicine, Biologia cel·lular, DNA damage, Science, 02 engineering and technology, MyoD, 03 medical and health sciences, medicine, Ciencias Biologicas, Fibroblast, Molecular Biology, Biologia molecular, 1000 Multidisciplinary, Biología molecular, Multidisciplinary, Nucleoplasm, Biología celular, Chemistry, Transdifferentiation, Cell Biology, Biological Sciences, Compartmentalization (psychology), Cell cycle, 021001 nanoscience & nanotechnology, 10226 Department of Molecular Mechanisms of Disease, Chromatin, Cell biology, Biología del desarrollo, 030104 developmental biology, medicine.anatomical_structure, Ciències biològiques, 570 Life sciences, biology, Biologia del desenvolupament, 0210 nano-technology, Developmental Biology
Abstract

While protein ADP-ribosylation was reported to regulate differentiation and dedifferentiation, it has so far not been studied during transdifferentiation. Here, we found that MyoD-induced transdifferentiation of fibroblasts to myoblasts promotes the expression of the ADP-ribosyltransferase ARTD1. Comprehensive analysis of the genome architecture by Hi-C and RNA-seq analysis during transdifferentiation indicated that ARTD1 locally contributed to A/B compartmentalization and coregulated a subset of MyoD target genes that were however not sufficient to alter transdifferentiation. Surprisingly, the expression of ARTD1 was accompanied by the continuous synthesis of nuclear ADP ribosylation that was neither dependent on the cell cycle nor induced by DNA damage. Conversely to the H2O2-induced ADP-ribosylation, the MyoD-dependent ADP-ribosylation was not associated to chromatin but rather localized to the nucleoplasm. Together, these data describe a MyoD-induced nucleoplasmic ADP-ribosylation that is observed particularly during transdifferentiation and thus potentially expands the plethora of cellular processes associated with ADP-ribosylation. Research in Beato's lab is supported by funds from the European Research Council under the European Union's Seventh Framework Program (FP7/2007-2013)/ERC Synergy grant agreement 609989 (4DGenome), from the Spanish Ministry of Economy and Competitiveness, ‘Centro de Excelencia Severo Ochoa 2013-2017’ and Plan Nacional (SAF2016-75006-P), as well as support of the CERCA Program/Generalitat de Catalunya. L.B was supported by the Forschungskredit 2019 of the University of Zurich and K.G by a grant of the Oncosuisse (Nr. KFS-3740-08-2015-R). ADP-ribosylation research in the laboratory of MOH is funded by the Kanton of Zurich and the Swiss National Science Foundation, Switzerland (grant 31003A_176177).

Published
2020

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