Search

Additional file 1. Supplementary Figure 1. The oligomerisation of CP12 is independent of the His-tag and does not involve disulfide bond. a Normalised SEC profile of recombinant His-tagged CP12 with (red) and without (blue) 1mM TCEP recorded on a Superdex 200 Increase 10 mm x 300 mm. Under reducing condition, His-tagged CP12 was treated with 10 mM TCEP before injection onto the column. b Normalised SEC profile of recombinant CP12 before (blue) and after His-tag removal (red) by thrombin, recorded on an Agilent Bio-SEC-3 300 Å column. Supplementary Table 1: Percentage of α-helices, strand, turn and other type of secondary structures of CP12 (included unstructured) derived from the CD profiles using different deconvolution methods: Bestsel, Dichroweb using the CDSSTR, SELCON3 and CONTIN methods and the reference set 7.