3,190 results on '"Pseudogenes"'
Search Results
2. Dual role of pseudogene TMEM198B in promoting lipid metabolism and immune escape of glioma cells
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Ying Zhan, Wei Qiao, Bolong Yi, Xinyu Yang, Miaomiao Li, Lu Sun, Lian Ji, Peng Su, Xin Wang, Furong Zhang, Rui Zhang, Mingjun Gao, Wujun Zhao, and Yichen Song
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Cancer Research ,Fatty Acid Elongases ,Lysine ,Fatty Acids ,RNA-Binding Proteins ,Glioma ,Lipid Metabolism ,Lipids ,DNA-Binding Proteins ,Histones ,ATP Citrate (pro-S)-Lyase ,Tumor Microenvironment ,Genetics ,Humans ,Molecular Biology ,Pseudogenes ,Transcription Factors - Abstract
Dysregulation of pseudogenes, enhancement of fatty acid synthesis and formation of immunosuppressive microenvironment are important factors that promote the malignant progression of glioma. It is of great significance to search for the molecular mechanism of interaction between the three and then perform targeted interference for improving the treatment of glioma. In this study, we found that pseudogene transmembrane protein 198B (TMEM198B) was highly expressed in glioma tissues and cell lines, and it could promote malignant progression of glioma by regulating lipid metabolism reprogramming and remodeling immune microenvironment. Applying the experimental methods of gene interference, lipidomics and immunology, we further confirmed that TMEM198B promoted PLAG1 like zinc finger 2 (PLAGL2) expression by mediating tri-methylation of histone H3 on lysine 4 (H3K4me3) of PLAGL2 through binding to SET domain containing 1B (SETD1B). Increased PLAGL2 could transcriptional activate ATP citrate lyase (ACLY) and ELOVL fatty acid elongase 6 (ELOVL6) expression, and then influenced the biological behaviors of glioma cells via enhancing the de novo lipogenesis and fatty acid acyl chain elongation. At the same time, TMEM198B promoted macrophages lipid accumulation and intensification of fatty acid oxidation (FAO) through glioma-derived exosomes (GDEs), further induced macrophages to M2 polarization, which subsequently facilitated immune escape of glioma cells. In conclusion, our present study clarifies that the TMEM198B/PLAGL2/ACLY/ELOVL6 pathway conducts crucial regulatory effects on the malignant progression of glioma, which provides novel targets and new ideas for molecular targeted therapy and immunotherapy of glioma.
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- 2022
3. Do pseudogenes pose a problem for metabarcoding marine animal communities?
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Jessica A. Schultz and Paul D. N. Hebert
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Electron Transport Complex IV ,Codon, Terminator ,Genetics ,Animals ,DNA, Environmental ,DNA, Mitochondrial ,Phylogeny ,Pseudogenes ,Ecology, Evolution, Behavior and Systematics ,Biotechnology - Abstract
Because DNA metabarcoding typically employs sequence diversity among mitochondrial amplicons to estimate species composition, nuclear mitochondrial pseudogenes (NUMTs) can inflate diversity. This study quantifies the incidence and attributes of NUMTs derived from the 658-bp barcode region of cytochrome c oxidase I (COI) in 156 marine animal genomes. NUMTs were examined to ascertain if they could be recognized by their possession of indels or stop codons. In total, 309 NUMTs ≥150 bp were detected, with an average of 1.98 per species (range = 0-33) and a mean length of 391 ± 200 bp. Among this total, 75 (24.3%) lacked indels or stop codons. NUMTs appear to pose the greatest interpretational risk when short (313 bp) amplicons are used, such as in environmental DNA studies, dietary analyses or processed fish identification. Employing the standard amplicon length (313 bp) for marine metabarcoding, NUMTs could potentially inflate the operational taxonomic unit (OTU) count by 21% above the true species count while also raising intraspecific variation at COI by 15%. However, when both amplicon length and position are considered, inflation in OTU counts and in barcode variation were just 9% and 10%, respectively, suggesting NUMTs will not seriously distort biodiversity assessments. There was a weak positive correlation between genome size and NUMT count but no variation among phyla or trophic groups. Until bioinformatic advances improve NUMT detection, the best defence involves targeting long amplicons and developing reference databases that include both mitochondrial sequences and their NUMT derivatives.
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- 2022
4. Long noncoding RNA (lncRNA) metallothionein 1 J, pseudogene (MT1JP) is downregulated in triple-negative breast cancer and upregulates microRNA-138 (miR-138) to downregulate hypoxia-inducible factor-1α (HIF-1α)
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Gangyue Wang, Yi Dong, Heng Liu, Nang Ji, Jilei Cao, Aihui Liu, Xin Tang, and Yu Ren
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Triple Negative Breast Neoplasms ,Bioengineering ,General Medicine ,Hypoxia-Inducible Factor 1, alpha Subunit ,Applied Microbiology and Biotechnology ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Cell Line, Tumor ,Humans ,Metallothionein ,RNA, Long Noncoding ,Pseudogenes ,Cell Proliferation ,Biotechnology - Abstract
Triple-negative breast cancer (TNBC) is a highly invasive subtype of breast cancer. This study explored the molecular mechanism and influences of metallothionein 1 J, pseudogene (MT1JP), microRNA-138 (miR-138), and hypoxia-inducible factor-1α (HIF-1α) on TNBC cell proliferation and migration. We confirmed TNBC cases by immunohistochemistry (IHC) staining. The expression of MT1JP in two types of tissue collected from 78 TNBC patients was detected by performing real-time quantitative fluorescence PCR (RT-qPCR). To further evaluate the relationship among MT1JP, miR-138 and HIF-1α, expression vectors of MT1JP and HIF-1α, as well as miR-138 mimic and inhibitor, were delivered into BT-549 cells. We observed that MT1JP was downregulated in TNBC. MT1JP was positively correlated with miR-138 but negatively correlated with HIF-1α in TNBC tissues. In TNBC cells, upregulation of miR-138 and downregulation of HIF-1α were observed after overexpression of MT1JP. In addition, overexpression of miR-138 resulted in downregulation of HIF-1α but did not affect the expression of MT1JP. Decreased proliferation rate of TNBC cells was observed after overexpression of MT1JP and miR-138. HIF-1α increased cell proliferation and migration. HIF-1α also suppressed the role of MT1JP and miR-138 in TNBC cell proliferation and migration. In conclusion, our findings demonstrated that MT1JP inhibited TNBC by regulating the miR-138/HIF-1α axis, indicating that MT1JP might serve as a biomarker or target for TNBC treatment.
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- 2022
5. The genome of the oomycetePeronosclerospora sorghi, a cosmopolitan pathogen of maize and sorghum, is inflated with dispersed pseudogenes
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Kyle Fletcher, Frank Martin, Thomas Isakeit, Keri Cavanaugh, Clint Magill, Richard Michelmore, and Jamann, T
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Peronospora ,mitogenome ,Prevention ,synteny ,Human Genome ,RNAseq ,Zea mays ,Oomycetes ,centromere ,whole-genome sequencing ,Genetics ,chromosome ,Edible Grain ,Molecular Biology ,Sorghum ,Pseudogenes ,Genetics (clinical) ,Plant Diseases ,Biotechnology - Abstract
Several species in the oomycete genus Peronosclerospora cause downy mildew on maize and can result in significant yield losses in Asia. Biosurveillance of these pathogens is a high priority to prevent epidemics on maize in the US and consequent damage to the US economy. The unresolved taxonomy and dearth of molecular resources for Peronosclerospora spp. hinder these efforts. P. sorghi is a pathogen of sorghum and maize with a global distribution, for which limited diversity has been detected in the southern USA. We characterized the genome, transcriptome, and mitogenome of an isolate, representing the US pathotype 6. The highly homozygous genome was assembled using 10x Genomics linked reads and scaffolded using Hi-C into 13 chromosomes. The total assembled length was 319.6 Mb—larger than any other oomycete previously assembled. The mitogenome was 38 kb, similar in size to other oomycetes, although it had a unique gene order. Nearly 20,000 genes were annotated in the nuclear genome, more than described for other downy mildew causing oomycetes. The 13 chromosomes of P. sorghi were highly syntenic with the 17 chromosomes of Peronospora effusa with conserved centromeric regions and distinct chromosomal fusions. The increased assembly size and gene count of P. sorghi is due to extensive retrotransposition, resulting in putative pseudogenization. Ancestral genes had higher transcript abundance and were enriched for differential expression. This study provides foundational resources for analysis of Peronosclerospora and comparisons to other oomycete genera. Further genomic studies of global Peronosclerospora spp. will determine the suitability of the mitogenome, ancestral genes, and putative pseudogenes for marker development and taxonomic relationships.
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- 2023
6. Pseudogene SNRPFP1 derived long non-coding RNA facilitates hepatocellular carcinoma progress in vitro by sponging tumor-suppressive miR-126-5p
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Nan, Wang, Simin, Guo, Fengjie, Hao, Yifan, Zhang, Yongjun, Chen, Xiaochun, Fei, and Junqing, Wang
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Gene Expression Regulation, Neoplastic ,MicroRNAs ,Carcinoma, Hepatocellular ,Multidisciplinary ,Cell Movement ,Cell Line, Tumor ,Liver Neoplasms ,Humans ,RNA, Long Noncoding ,Pseudogenes ,Cell Proliferation - Abstract
Pseudogene-derived transcripts, especially those barely transcribed in normal tissues, have been regarded as a kind of non-coding RNAs, and present potential functions in tumorigenicity and tumor development in human beings. However, their exact effects on hepatocellular carcinoma (HCC) remain largely unknown. On basis of our previous research and the constructed online database for the non-coding RNAs related to HCC, a series of pseudogene transcripts have been discovered, and SNRPFP1, the homologous pseudogene of SNRPF, was found to produce an anomalously high expression long non-coding RNA in HCC. In this study, we validated the expression of the SNRPFP1 transcript in both HCC tissues and cell lines. The adverse correlation between SNRPFP1 expression and patients’ outcomes was observed. And depletion of SNRPF1 in HCC cells significantly suppressed cell proliferation and apoptosis resistance. Meanwhile, the motility of HCC cells was potently impaired. Interestingly, miR-126-5p, one of the tumor-suppressive genes commonly decreased in HCC, was found negatively expressed and correlated with SNRPF1, and a specific region of SNRPF1 transcript is directly binding to miR-126-5p in a molecular sponge way. The rescue experiment by knock-out miR-126-5p significantly reversed the cell growth suppression and a higher ratio of cell apoptosis induced by SNRPF1 depletion. Lastly, we concluded that SNRPF1 is a pseudogene active in HCC, and its abnormally over-expressed transcript is a strong promoter of HCC cell progress in vitro by sponging miR-126-5p. We believe that the findings in this study provide new strategies for HCC prevention and therapeutic treatment.
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- 2022
7. Dysregulated pseudogene <scp>BNIP3P1</scp> inhibited cell proliferation and promoted cell apoptosis in preeclampsia by acting as a competing endogenous <scp>RNA</scp> for <scp>BNIP3</scp>
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Haoyu Zheng, Mengna Shi, Zhenjing Chi, Haidong Wang, Hairong Wang, and Dong Xu
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Health, Toxicology and Mutagenesis ,Membrane Proteins ,Apoptosis ,General Medicine ,Management, Monitoring, Policy and Law ,Toxicology ,MicroRNAs ,Pre-Eclampsia ,Cell Movement ,Pregnancy ,Proto-Oncogene Proteins ,Humans ,Female ,RNA, Long Noncoding ,Pseudogenes ,Cell Proliferation - Abstract
Long noncoding RNAs (lncRNAs) have been reported as critical modulators in many diseases including preeclampsia. Since the association between lncRNA BNIP3P1 and its cognate gene BNIP3 in preeclampsia has been revealed previously, this study aimed to further explore the function and mechanism of BNIP3P1 in preeclampsia. EdU and TUNEL assays revealed that BNIP3P1 or BNIP3 overexpression inhibited trophoblast cell proliferation and enhanced cell apoptosis in preeclampsia. As suggested by western blot analysis, the protein levels of apoptotic markers in the cells were affected by BNIP3P1 or BNIP3 overexpression. The binding between miR-128-3p and BNIP3P1 (or BNIP3) was explored by luciferase reporter assays. Mechanistically, BNIP3P1 bound to miR-128-3p to upregulate BNIP3 expression by acting as a competing endogenous RNA (ceRNA). Importantly, BNIP3P1 was found to inactivate the mTOR signaling pathway. In conclusion, BNIP3P1 inhibited trophoblast cell proliferation and enhanced cell apoptosis in preeclampsia by targeting the miR-128-3p/BNIP3/mTOR signaling pathway.
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- 2022
8. Long non-coding RNA TPTEP1 exerts inhibitory effects on hepatocellular carcinoma by impairing microRNA-454-3p-mediated DLG5 downregulation
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Yuandi Dong, Haishi Liu, Jianmin Sun, Qingshan Wang, and Haiyang Wang
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Carcinoma, Hepatocellular ,Carcinogenesis ,Down-Regulation ,medicine.disease_cause ,Mice ,Downregulation and upregulation ,Cell Movement ,microRNA ,Biomarkers, Tumor ,medicine ,Animals ,Humans ,Neoplasm Invasiveness ,Cell Proliferation ,Hepatology ,Akt/PKB signaling pathway ,Cell growth ,business.industry ,Tumor Suppressor Proteins ,Liver Neoplasms ,PTEN Phosphohydrolase ,Gastroenterology ,Membrane Proteins ,medicine.disease ,digestive system diseases ,Long non-coding RNA ,Up-Regulation ,Disease Models, Animal ,MicroRNAs ,Protein kinase B signaling ,Hepatocellular carcinoma ,Cancer research ,RNA, Long Noncoding ,business ,Proto-Oncogene Proteins c-akt ,Pseudogenes ,Signal Transduction - Abstract
Background Hepatocellular carcinoma (HCC) is usually diagnosed at late stages, making it the second cause of malignancy-related death across the world. Long noncoding RNAs (lncRNAs) are of significance to tumorigenesis, highly suggestive of their functional roles as novel biomarkers for cancer therapy. The current study investigated the specific role of lncRNA TPTE pseudogene 1 (TPTEP1) in HCC. Methods Expression of lncRNA TPTEP1, microRNA-454-3p (miR-454-3p) and discs large homolog 5 (DLG5) was determined in tissues samples from the recruited patients with HCC. Cell proliferation, migration and invasion assays were performed to determine effects of lncRNA TPTEP1, miR-454-3p and DLG5 on the malignant phenotype of tumor cells. Finally, the mouse HCC model was also established to disclose the tumor suppressor effects of lncRNA TPTEP1 in vivo. Results LncRNA TPTEP1 was downregulated both in HCC cells and tissues, and played a negative regulatory role in HCC cell proliferation, migration and invasion. Mechanistically, lncRNA TPTEP1 competitively bound to miR-454-3p, thereby upregulating its endogenous target DLG5. Moreover, lncRNA TPTEP1 hindered activation of the protein kinase B signaling pathway, causing inhibited malignant phenotypes of HCC cells. Also, lncRNA TPTEP1 suppressed tumor growth and extrahepatic metastasis (lung) via miR-454-3p/DLG5 axis. Conclusion Taken together, this research revealed a concrete mechanism of lncRNA TPTEP1 in HCC.
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- 2022
9. Functions of lncRNA DUXAP8 in non-small cell lung cancer
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Cui Wu, Wu Song, Zhongnan Wang, and Bingmei Wang
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Gene Expression Regulation, Neoplastic ,MicroRNAs ,Lung Neoplasms ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,Genes, Homeobox ,Genetics ,Humans ,RNA, Long Noncoding ,General Medicine ,Molecular Biology ,Pseudogenes ,Cell Proliferation - Abstract
Non-small cell lung cancer (NSCLC) poses a serious threat to public health due to its significant morbidity and mortality rates. The processes of NSCLC formation and development are quite complex and involve numerous regulatory biomolecules. Long non-coding RNAs (lncRNAs) have attracted attention since they have been found to play critical roles in the tumorigenesis of various human malignancies. Recently, double homeobox A pseudogene 8 (DUXAP8) was identified as an oncogenic lncRNA that is overexpressed in different tumor types. In NSCLC, high expression of DUXAP8 is associated with poor prognosis in patients. The regulatory mechanism underlying the oncogenic effects of DUXAP8 can be divided into transcriptional level and post-transcriptional level. DUXAP8 promotes proliferation, epithelial-mesenchymal transition, and aerobic glycolysis in NSCLC cells. Moreover, DUXAP8 shows potential for the diagnosis and treatment of NSCLC. Herein, we review the molecular mechanisms underlying the DUXAP8-mediated phenotypes of NSCLC as well as its potential clinical applications.
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- 2022
10. N6-methyladenosine-associated prognostic pseudogenes contribute to predicting immunotherapy benefits and therapeutic agents in head and neck squamous cell carcinoma
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Liqiang Tan, Yali Qin, Ruiling Xie, Tianliang Xia, Xiaotong Duan, Lan Peng, Rui You, Youping Liu, Xiong Zou, Mengxia Zhang, Mei Lin, and Mingyuan Chen
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MicroRNAs ,Adenosine ,Head and Neck Neoplasms ,Squamous Cell Carcinoma of Head and Neck ,Humans ,Immunologic Factors ,Medicine (miscellaneous) ,Immunotherapy ,Prognosis ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Pseudogenes - Published
- 2022
11. HLA-H*02:07 Is a Membrane-Bound Ligand of Denisovan Origin That Protects against Lysis by Activated Immune Effectors
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Lucas Hubert, Julien Paganini, Christophe Picard, Jacques Chiaroni, Laurent Abi-Rached, Pierre Pontarotti, Julie Di Cristofaro, Aix Marseille Université (AMU), Anthropologie bio-culturelle, Droit, Ethique et Santé (ADES), Aix Marseille Université (AMU)-EFS ALPES MEDITERRANEE-Centre National de la Recherche Scientifique (CNRS), Etablissement Français du Sang Provence-Alpes Côte-d'Azur et Corse (EFS), XEGEN, 15 rue de la République, 13420, Gémenos, Assistance Publique - Hôpitaux de Marseille (APHM), Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), and Centre National de la Recherche Scientifique (CNRS)
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Cytotoxicity, Immunologic ,Genotype ,[SDV]Life Sciences [q-bio] ,Immunology ,Lymphocyte Activation ,White People ,HLA-A11 Antigen ,Evolution, Molecular ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Gene Frequency ,Immune Tolerance ,Homeostasis ,Humans ,Immunology and Allergy ,Hemochromatosis Protein ,Alleles ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,0303 health sciences ,Cell Membrane ,3. Good health ,Killer Cells, Natural ,Haplotypes ,030220 oncology & carcinogenesis ,K562 Cells ,Pseudogenes - Abstract
The biological relevance of genes initially categorized as “pseudogenes” is slowly emerging, notably in innate immunity. In the HLA region on chromosome 6, HLA-H is one such pseudogene; yet, it is transcribed, and its variation is associated with immune properties. Furthermore, two HLA-H alleles, H*02:07 and H*02:14, putatively encode a complete, membrane-bound HLA protein. Here we thus hypothesized that HLA-H contributes to immune homeostasis similarly to tolerogenic molecules HLA-G, -E, and -F. We tested if HLA-H*02:07 encodes a membrane-bound protein that can inhibit the cytotoxicity of effector cells. We used an HLA-null human erythroblast cell line transduced with HLA-H*02:07 cDNA to demonstrate that HLA-H*02:07 encodes a membrane-bound protein. Additionally, using a cytotoxicity assay, our results support that K562 HLA-H*02:07 inhibits human effector IL-2–activated PBMCs and human IL-2–independent NK92-MI cell line activity. Finally, through in silico genotyping of the Denisovan genome and haplotypic association with Denisovan-derived HLA-A*11, we also show that H*02:07 is of archaic origin. Hence, admixture with archaic humans brought a functional HLA-H allele into modern European and Asian populations.
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- 2022
12. The extracellular vesicular pseudogene LGMNP1 induces M2-like macrophage polarization by upregulating LGMN and serves as a novel promising predictive biomarker for ovarian endometriosis recurrence
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S G Sun, J J Guo, X Y Qu, X Y Tang, Y Y Lin, K Q Hua, and J J Qiu
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China ,Adolescent ,Macrophages ,Rehabilitation ,Endometriosis ,Obstetrics and Gynecology ,Cysteine Endopeptidases ,Endometrium ,Extracellular Vesicles ,Mice ,Reproductive Medicine ,Animals ,Humans ,Female ,RNA, Messenger ,Stromal Cells ,Biomarkers ,Pseudogenes ,Retrospective Studies - Abstract
STUDY QUESTION How does ectopic endometrial stromal cell (Ecto-ESC)-derived extracellular vesicular Legumain pseudogene 1 (EV-LGMNP1), a newly identified pseudogene of Legumain (LGMN), contribute to M2-phenotype macrophage polarization, and does it predict recurrence in patients with ovarian endometriosis (EMs)? SUMMARY ANSWER EV-LGMNP1, which is abundant in Ecto-ESCs and serum from ovarian EMs, can direct macrophages towards an M2 phenotype by upregulating LGMN expression and is a promising biomarker for predicting ovarian EMs recurrence. WHAT IS KNOWN ALREADY Extracellular vesicles (EVs) can mediate cell-to-cell crosstalk to promote disease progression via cargo molecule transport. Recently, LGMNP1, a newly identified pseudogene of LGMN, has been reported to promote cancer progression by upregulating LGMN. LGMN is a well-studied protein that can induce M2-like polarization. STUDY DESIGN, SIZE, DURATION An in vitro study was conducted with Ecto-ESCs isolated from ectopic endometrial samples, collected from two patients with ovarian EMs (diagnosed by laparoscopy and histological analysis). A clinical retrospective cohort study of 52 ovarian EMs patients and 21 controls with available preoperative serum samples was carried out (2013–2017). The follow-up period ended either at the time of recurrence or on 31 December 2018. PARTICIPANTS/MATERIALS, SETTING, METHODS Ecto-ESC-derived EVs (EV/Ecto-ESCs) were characterized by nanoparticle tracking analysis, transmission electron microscopy and western blotting. EV internalization by THP-1 cells, which are the most widely used primary human macrophages model, was detected by fluorescence labelling. After EV treatment, THP-1 cell polarization was detected by quantitative real-time PCR (qRT-PCR) and western blot analyses of CD86 (M1-related marker) and CD206 (M2-related marker). LGMNP1 mRNA expression level in EVs from both primary ectopic endometrioc stromal cells and serum was examined using qRT-PCR. Additionally, the expression of LGMN, the downstream target gene of LGMNP1, in THP-1 cells was evaluated using qRT-PCR and western blotting. Kaplan–Meier and multivariate Cox regression analyses were applied to evaluate the independent predictive factors of EMs recurrence-free survival. A novel nomogram model based on serum EV-LGMNP1 was then formulated to predict EMs recurrence. MAIN RESULTS AND THE ROLE OF CHANCE In vitro assays demonstrated that EV/Ecto-ESCs drove macrophages towards an M2-like phenotype. Moreover, LGMNP1 contributed to EV/Ecto-ESC-induced M2 macrophage polarization by upregulating LGMN mRNA expression levels. Clinically, serum EV-LGMNP1 was more highly expressed in recurrent EMs patients than in controls and EMs patients without recurrence. Survival analysis and our novel nomogram reconfirmed that serum EV-LGMNP1 was a novel promising and meaningful non-invasive biomarker for predicting EMs recurrence. LARGE SCALE DATA N/A. LIMITATIONS, REASONS FOR CAUTION In vitro experiments were only performed on samples from two patients with ovarian endometriosis, and a larger sample size is needed. ESCs isolated from the eutopic endometrium of EMs and non-EMs patients should be studied in the future. Additionally, in vitro experiments should be performed using endometrial epithelium cells and further in vivo experiments, such as using mice endometriotic models to investigate whether EV/Ecto could induce M2 macrophage polarization, should be conducted. Moreover, multicentre, large-sample data are needed to validate our predictive nomogram model. WIDER IMPLICATIONS OF THE FINDINGS Our study provides novel insights into the mechanism of M2 polarization involved in ovarian EMs progression mediated by an ‘EV-shuttled pseudogene LGMNP1’ mode. In addition, serum EV-LGMNP1 may serve as a novel non-invasive biomarker for predicting recurrence, providing a new therapeutic target for ovarian EMs. STUDY FUNDING/COMPETING INTEREST(S) This project was supported by funding from the National Natural Science Foundation of China (81971361), the Natural Science Foundation of Shanghai Science and Technology (19ZR1406900), the Shanghai ‘Rising Stars of Medical Talent’ Youth Development Program (AB83030002019004), the Clinical Research Plan of SHDC (SHDC2020CR4087), the Shanghai Municipal Health Commission (202040498), the Research and Innovation Project of the Shanghai Municipal Education Commission (2019-01-07-00-07-E00050) and the Clinical Research Plan of SHDC (SHDC2020CR1045B). There are no competing interests to declare.
- Published
- 2021
13. The Pseudogene BMEA_B0173 Deficiency in Brucella melitensis Contributes to M-epitope Formation and Potentiates Virulence in a Mice Infection Model
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Ge, Zhang, Hao, Dong, Yu, Feng, Hui, Jiang, Tonglei, Wu, Jiali, Sun, Xin, Wang, Minghe, Liu, Xiaowei, Peng, Yinghui, Zhang, Xiaoqian, Zhang, Liangquan, Zhu, Jiabo, Ding, and Xingjia, Shen
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Mice ,Epitopes ,Disease Models, Animal ,Virulence ,Bacterial Proteins ,Brucella melitensis ,Animals ,General Medicine ,Applied Microbiology and Biotechnology ,Microbiology ,Pseudogenes ,Brucellosis - Abstract
It is widely acknowledged that pseudogenes play important roles in bacterial diversification and evolution and participate in gene regulation and RNA interference (RNAi). However, the function of most pseudogenes in Brucella spp remains poorly understood, warranting further studies.To comprehensively analyze the function of the pseudogenes BMEA_B0173 in Brucella melitensis strain 63/9, a BMEA_B0173 in-frame deleted mutant strain was constructed. Then, the phenotypes of the mutant strain, such as growth characteristics and bacterial virulence, were assessed in mice infection models. Finally, iTRAQ analysis was performed to investigate the gene expression profile affected by the pseudogenes BMEA_B0173. In this study, we found that BMEA_B0173 deletion exhibited increased agglutination with M monospecific sera. In a mouse model of chronic infection, the BMEA_B0173 deletion strain displayed increased colonization in the spleen compared to the wild-type pathogen. The iTRAQ assay revealed that 252 proteins were differentially expressed between the BMEA_B0173 deletion and the wild-type strains. In addition, deletion of BMEA_B0173 significantly increased the expression of proteins involved in the denitrification pathway, iron metabolism, and several transcriptional regulators, which might cause increased virulence of the mutant strain. In conclusion, this study preliminary uncovered the function of the pseudogene BMEA_B0173 in Brucella melitensis 63/9 and provided novel insights for studying the pathogenesis of Brucella strains.
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- 2022
14. The pseudogene DUXAP10 contributes to gefitinib resistance in NSCLC by repressing OAS2 expression
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Shengnan, Ren, Ya, Zhu, Siying, Wang, Qinqiu, Zhang, Niu, Zhang, Xiaoteng, Zou, Chenchen, Wei, and Zhaoxia, Wang
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Lung Neoplasms ,Biophysics ,Gefitinib ,Antineoplastic Agents ,General Medicine ,Biochemistry ,Ligases ,ErbB Receptors ,Drug Resistance, Neoplasm ,Cell Line, Tumor ,Carcinoma, Non-Small-Cell Lung ,Humans ,Protein Kinase Inhibitors ,Pseudogenes - Abstract
Gefitinib, an epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI),is the currently recommended first-line therapy for advanced EGFR-mutant lung cancer, and understanding the mechanism of resistance is the key to formulating therapeutic strategies for EGFR-TKIs. In this study, we evaluate the expression patterns and potential biological functions of the pseudogene DUXAP10 in gefitinib resistance. We find that pseudogene DUXAP10 expression is significantly upregulated in NSCLC gefitinib-resistant cells and tissues. Gain and loss of function assays reveal that knockdown of DUXAP10 by siRNA reverses gefitinib resistance both
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- 2022
15. Pseudogenes and the associated ceRNA network as potential prognostic biomarkers for colorectal cancer
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Zhuoqi, Li, Jing, Zhou, Liankun, Gu, and Baozhen, Zhang
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Gene Expression Regulation, Neoplastic ,MicroRNAs ,Multidisciplinary ,Colonic Neoplasms ,Humans ,RNA, Long Noncoding ,Gene Regulatory Networks ,RNA, Messenger ,Adenocarcinoma ,Prognosis ,Pseudogenes ,Biomarkers - Abstract
Colorectal cancer (CRC) is one of the most common and malignant carcinomas. Many long noncoding RNAs (lncRNAs) have been reported to play important roles in the tumorigenesis of CRC by influencing the expression of some mRNAs via competing endogenous RNA (ceRNA) networks and interacting with miRNAs. Pseudogene is one kind of lncRNA and can act as RNA sponges for miRNAs and regulate gene expression via ceRNA networks. However, there are few studies about pseudogenes in CRC. In this study, 31 differentially expressed (DE) pseudogenes, 17 DE miRNAs and 152 DE mRNAs were identified by analyzing the expression profiles of colon adenocarcinoma obtained from The Cancer Genome Atlas. A ceRNA network was constructed based on these RNAs. Kaplan–Meier analysis showed that 7 pseudogenes, 4 miRNAs and 30 mRNAs were significantly associated with overall survival. Then multivariate Cox regression analysis of the ceRNA-related DE pseudogenes was performed and a 5-pseudogene signature with the greatest prognostic value for CRC was identified. Moreover, the results were validated by the Gene Expression Omnibus database, and quantitative real-time PCR in 113 pairs of CRC tissues and colon cancer cell lines. This study provides a pseudogene-associated ceRNA network, 7 prognostic pseudogene biomarkers, and a 5-pseudogene prognostic risk signature that may be useful for predicting the survival of CRC patients.
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- 2022
16. Association of Inherited Copy Number Variation in
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Juliana, Carron, Caroline, Torricelli, Janet Keller, Silva, Yichuan, Liu, Renata, Pellegrino, Carmen Silvia Passos, Lima, and Gustavo Jacob, Lourenço
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MicroRNAs ,Oropharyngeal Neoplasms ,ADAM Proteins ,DNA Copy Number Variations ,Squamous Cell Carcinoma of Head and Neck ,Head and Neck Neoplasms ,Humans ,Membrane Proteins ,Pseudogenes - Abstract
Inherited copy number variations (CNVs) can provide valuable information for cancer susceptibility and prognosis. However, their association with oropharynx squamous cell carcinoma (OPSCC) is still poorly studied. Using microarrays analysis, we identified three inherited CNVs associated with OPSCC risk, of which one was validated in 152 OPSCC patients and 155 controls and related to pseudogene-microRNA-mRNA interaction. Individuals with three or more copies of
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- 2022
17. The diagnostic and prognostic value of pseudogene SIGLEC17P in lung adenocarcinoma and a preliminary functional study
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Xiuqing Shen, Yanfen Zeng, Caihong Yang, Lili Jiang, Shaoting Chen, Falin Chen, and Pengju Cao
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Gene Expression Regulation, Neoplastic ,MicroRNAs ,Lung Neoplasms ,Cell Movement ,Cell Line, Tumor ,Humans ,Cell Biology ,General Medicine ,Adenocarcinoma ,Prognosis ,Lung ,Pseudogenes ,Cell Proliferation - Abstract
Among malignant tumors, lung adenocarcinoma (LUAD) is the leading cause of death worldwide. This study explored the diagnostic, prognostic value, and preliminary functional verification of sialic acid binding Ig like lectin 17, pseudogene (SIGLEC17P) in LUAD. Prognostic lncRNAs for LUAD were identified by The Cancer Genome Atlas and quantitative real-time PCR (qRT-PCR) was used to detect the expression of SIGLEC17P in LUAD and paracarcinoma tissues. Subsequently, lentiviral vectors were used to overexpress SIGLEC17P in A549 and H1299 cells. The effects of SIGLEC17P overexpression on the proliferation, migration, and invasiveness of LUAD cells (A549 and H1299) were evaluated by Cell Counting Kit-8, wound healing, and transwell migration assays, respectively. Bioinformatics analyses were performed to reveal the potential pathways in which SIGLEC17P is involved in LUAD. qRT-PCR results revealed low SIGLEC17P expression in LUAD tissues and a significant association with the N stage, T stage, and tumor node metastasis stage. Furthermore, the receiver operating characteristic curve demonstrated a reliable diagnostic value. The proliferation, migration, and invasion of LUAD cells were inhibited by overexpression of SIGLEC17P. Bioinformatics analyses suggested that SIGLEC17P might exert antioncogenic effects in LUAD through the mir-20-3p/ADH1B or mir-4476-5p/DPYSL axis. In summary, our results revealed that SIGLEC17P acts as a prognostic biomarker, independent prognostic factor, and potential therapeutic target for patients with LUAD.
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- 2022
18. Lack of CpG islands in human unitary pseudogenes and its implication
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Ammad Aslam Khan, Ayesha Liaqat, Bisma Asghar, Farah Babar, Muhammad Awais Shafqat, Anees Fatima, Maheen Fatima, Nimra Ilyas, Muhammad Aslam Gondal, and Muhammad Shahryar Ali
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Mammals ,Genome ,Pseudogene ,Context (language use) ,Promoter ,DNA Methylation ,Biology ,Human genetics ,Housekeeping gene ,CpG site ,Evolutionary biology ,Genetics ,Animals ,Humans ,CpG Islands ,Promoter Regions, Genetic ,Gene ,Pseudogenes - Abstract
CpG islands (CGIs) are aggregation of CpG dinucleotides in the promoters of mammalian genes. These CGIs are present in almost all the housekeeping genes and some tissue-specific genes in the mammalian genome. Extensive research has been done on the prevalence and role of CGIs in protein-coding genes. However, little is known about CGIs in pseudogenes. In the current research project, we focused on CGIs in three main classes of pseudogenes e.g., duplicated pseudogenes (DPGs), processed pseudogenes (PPGs), and unitary pseudogenes (UPGs). We discovered a predominant absence of CGIs in the promoters of all three pseudogenes. We also compared the CGI profile of these pseudogenes with their parent genes and found that unitary pseudogenes (UPGs) differ from the DPGs and PPGs in the sense that in the latter, lack of CGIs is a consequential event while in UPGs, this lack of CGIs in their promoters is not a result of pseudogenization process. We also discussed the implication of the results obtained from this comparison. To our knowledge, this is the first-ever study highlighting this aspect of UPGs throwing new insights into the evolution of genome in general and especially in the context of pseudogenes.
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- 2021
19. Construction of a lncRNA/pseudogene-hsa-miR-30d-5p-GJA1 regulatory network related to metastasis of pancreatic cancer
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Huilin Zheng, Weiyang Lou, Ke Xue, Bisha Ding, and Jinxiao Yu
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0106 biological sciences ,Pseudogene ,Cell ,Biology ,01 natural sciences ,Metastasis ,03 medical and health sciences ,Pancreatic cancer ,microRNA ,Gene expression ,Genetics ,medicine ,Humans ,Survival analysis ,030304 developmental biology ,0303 health sciences ,CTNND1 ,medicine.disease ,Gene Expression Regulation, Neoplastic ,Pancreatic Neoplasms ,MicroRNAs ,medicine.anatomical_structure ,Connexin 43 ,Cancer research ,RNA, Long Noncoding ,Transcriptome ,Pseudogenes ,010606 plant biology & botany - Abstract
Pancreatic cancer, the most lethal malignant tumor, is notorious for its poor prognosis and metastatic potential. Non-coding RNAs (ncRNAs) are reported to play key roles in cancer metastasis. In this study, miRNA and gene expression profiles between metastatic pancreatic cancer cell M8 and its parental cell BxPC.3 were determined. Using differential expression analysis, survival analysis, target gene prediction, pathway enrichment analysis, intersection analysis and correlation analysis, hsa-miR-30d-5p/GJA1 axis was identified as the most potential pathway involved in metastasis of pancreatic cancer. Subsequently, two upstream lncRNAs (HELLPAR and OIP-AS1) and four upstream pseudogenes (AC093616.1, AC009951.1, TMEM183B and PABPC1P4) of hsa-miR-30d-5p/GJA1 axis were predicted and were then identified via assessment of RNA-RNA expression relationship. Furthermore, CTNNA1, CTNNB1 and CTNND1 were regarded as three crucial molecules to be participated in hsa-miR-30d-5p/GJA1-mediated metastatic potential in pancreatic cancer. In conclusion, we established a novel lncRNA/pseudogene-hsa-miR-30d-5p-GJA1 regulatory network linked to metastasis of pancreatic cancer.
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- 2021
20. X-linked recessive ichthyosis in 8 Tunisian patients: awareness of misdiagnosis due to the technical trap of the STS pseudogene
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Hamza Chouk, Sarra Saad, Sarra Dimassi, Nadia Ghariani Fetoui, Ayda Bennour, Rima Gammoudi, Haifa Elmabrouk, Ali Saad, Mohamed Denguezli, and Dorra H’mida
- Subjects
Male ,Heterozygote ,Ichthyosis, X-Linked ,Codon, Nonsense ,Genetics ,Humans ,Steryl-Sulfatase ,Diagnostic Errors ,Pseudogenes ,Genetics (clinical) - Abstract
Introduction X-linked recessive ichthyosis (XLI) is a genodermatosis, caused by a deficiency of the steroid sulphatase enzyme encoded by the STS gene (OMIM # 300,747). Adopted XLI molecular diagnosis approaches differ from one laboratory to another depending on available technical facilities. Our work aims to figure out a sound diagnostic strategy for XLI. Patients and methods We collected 8 patients with XLI, all males, from 3 unrelated Tunisian families from central Tunisia. Genetic diagnosis was conducted through a large panel of genetic techniques including: Sanger Sequencing, haplotype analysis of STR markers, MLPA analysis, FISH and array CGH. Results Direct Sanger sequencing of the STS gene showed the same deletion of 13 base pairs within the exon 4 in all patients resulting in a premature stop codon. However, all patients’ mothers were not carriers of this variant and no common haplotype flanking STS gene was shared between affected patients. Sequence alignment with reference human genome revealed an unprocessed pseudogene of the STS gene located on the Y chromosome, on which the 13 bp deletion was actually located. STS MLPA analysis identified a deletion of the entire STS gene on X chromosome for all affected patients. This deletion was confirmed by FISH and delineated by array CGH. Conclusion All our patients shared a deletion of the entire STS gene revealed by MLPA, confirmed by FISH and improved by array CGH. Geneticists must be aware of the presence of pseudogenes that can lead to XLI genetic misdiagnosis.
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- 2022
21. Pseudofinder: Detection of Pseudogenes in Prokaryotic Genomes
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Arkadiy I. Garber, Filip Husnik, John P. McCutcheon, Mitch J Syberg-Olsen, and Patrick J. Keeling
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Bacteria ,archaea ,Pseudogene ,pseudogene ,Computational biology ,prediction ,Biology ,ENCODE ,Genome ,Population bottleneck ,annotation ,Prokaryotic Cells ,Genome, Archaeal ,Genetics ,dN/dS ,Identification (biology) ,Evolutionary dynamics ,Molecular Biology ,Gene ,genome ,Ecology, Evolution, Behavior and Systematics ,Selection (genetic algorithm) ,Pseudogenes ,Software - Abstract
Prokaryotic genomes are generally gene dense and encode relatively few pseudogenes, or nonfunctional/inactivated remnants of genes. However, in certain contexts, such as recent ecological shifts or extreme population bottlenecks (such as those experienced by symbionts and pathogens), pseudogenes can quickly accumulate and form a substantial fraction of the genome. Identification of pseudogenes is, thus, a critical step for understanding the evolutionary forces acting upon, and the functional potential encoded within, prokaryotic genomes. Here, we present Pseudofinder, an open-source software dedicated to pseudogene identification and analysis. With Pseudofinder’s multi-pronged, reference-based approach, we demonstrate its capacity to detect a wide variety of pseudogenes, including those that are highly degraded and typically missed by gene-calling pipelines, as well newly formed pseudogenes, which can have only one or a few inactivating mutations. Additionally, Pseudofinder can detect intact genes undergoing relaxed selection, which may indicate incipient pseudogene formation. Implementation of Pseudofinder in annotation pipelines will not only clarify the functional potential of sequenced microbes, but will also generate novel insights and hypotheses regarding the evolutionary dynamics of bacterial and archaeal genomes.
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- 2022
22. Differential expression of the three independent CaM genes coding for an identical protein: Potential relevance of distinct mRNA stability by different codon usage
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Mads Munk, Eduardo Villalobo, Antonio Villalobo, Martin W. Berchtold, Universidad de Sevilla. Departamento de Microbiología, Danish Research Council, and Ministerio de Ciencia e Innovación (MICIN). España
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Mammals ,Physiology ,RNA Stability ,Cell Biology ,Epigenetic control ,MicroRNAs ,Calmodulin ,Mutation-mediated diseases ,Animals ,Humans ,miRNA control ,Codon usage ,RNA, Messenger ,Calmodulin genes ,Codon ,Codon Usage ,Molecular Biology ,Phylogeny ,Pseudogenes - Abstract
The Ca2+-sensor protein calmodulin (CaM) is a major regulator of multiple cell functions. A unique and puzzling feature of human, and all so far investigated mammals, is the presence of three distinct CaM genes on different chromosomes, which code for identical proteins. How this case of apparent genetic redundancy evolved and why it could be to the advantage of the mammalian organisms is not well established. With a main focus on humans, this article aims to review existing literature addressing how the genes nonetheless differ in function. Clearly, the three CaM genes are differentially expressed in different tissues, during development, in response to different stimuli, and other factors including environmental conditions. As shown in hippocampal neurons, different mRNAs from the CAM genes may even localize differently within the same cell. Regulation of CaM gene expression is achieved by a variety of regulatory elements present in the three genes, including different promotor/insulator elements and 3′- and 5′-noncoding regions differing in length and sequence, as well as regulation by epigenetic factors and miRNAs. Here, we hypothesize that predicted differences in mRNA stability and translational efficiency due to divergent codon usage could play an additional regulatory role as the three genes differ markedly in their use of synonymous codons. CALM3, predicted to produce a relatively stable mRNA may be important where the transcription level is low or transiently absent, e.g. during spermatogenesis. In contrast, CALM2 with a predicted much shorter mRNA half-life, may provide better temporal control of CaM levels. Deciphering the underlying mechanisms responsible for all this complexity may help to understand why this unique multigenic arrangement may be an advantage for the optimal spatio-temporal expression of CaM in higher eukaryotes. Finally, we discuss the expression of the CaM genes in selected human pathologies, and how mutations in these genes are responsible for the appearance of serious congenital syndromes, mainly affecting the heart, and although less known, possibly also affecting the functionality of the central nervous system and other organs. Danish Research Council DFF-4004-00560 Ministerio de Ciencia e Innovación PID2019-103850GB-I00
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- 2022
23. Long Non-Coding RNA DUXAP8 Acts as an Oncogene in Sinonasal Squamous Cell Carcinoma Through miR-584-5p/FNDC3B Pathway
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Xuan Chen, Guidi Li, Guanzhong Zhong, Junyong Chen, Lijun Feng, Tao Zhang, and Zhi Tang
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Genes, Homeobox ,General Medicine ,Oncogenes ,Fibronectins ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Otorhinolaryngology ,Cell Movement ,DNA Nucleotidylexotransferase ,Cell Line, Tumor ,Carcinoma, Squamous Cell ,Immunology and Allergy ,Humans ,RNA, Long Noncoding ,Pseudogenes ,Cell Proliferation - Abstract
Sinonasal squamous cell carcinoma (SNSCC) is one of the least frequent carcinomas in the head and neck and accounts for 60% to 75% of sinonasal malignancies. The role of long non-coding RNAs (lncRNAs) in cancer development has drawn great attention over the years. The current study intended to assess the role and specific mechanism of lncRNA double homeobox A pseudogene 8 (DUXAP8) in SNSCC. Quantitative real-time PCR (qRT-PCR) analysis was implemented to assess the expression level of DUXAP8, microRNA-584-5p (miR-584-5p), and fibronectin type III domain containing 3B (FNDC3B). Proliferation assays included colony formation assay, Cell Counting Kit-8 (CCK-8) assay, and 5-ethynyl-2′-deoxyuridine (EdU) assay. Transwell assays were implemented to monitor cell migration and invasion. Cell apoptosis was evaluated via terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) and JC-1 experiments. Mechanism experiments included RNA pull-down assay, RNA binding protein immunoprecipitation (RIP) assay, and luciferase reporter assay. DUXAP8 is overexpressed in SNSCC cells. Functionally, DUXAP8 silencing suppresses the malignant progression of SNSCC. Furthermore, DUXAP8 up-regulates the expression of FNDC3B via sponging miR-584-5p. Rescue experiments demonstrated that DUXAP8 mediates the progression of SNSCC via up-regulating FNDC3B expression. In conclusion, DUXAP8 acts as an oncogene in SNSCC, which may be a new molecular marker for SNSCC.
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- 2022
24. Gauging the trends of pseudogenes in plants
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Kashmir Singh, Neetu Goyal, Shivalika Pathania, Jagdeep Kaur, and Naina Garewal
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0106 biological sciences ,Regulation of gene expression ,0303 health sciences ,Genome ,Pseudogene ,General Medicine ,Genome project ,Biology ,Biological Evolution ,01 natural sciences ,Applied Microbiology and Biotechnology ,Mice ,03 medical and health sciences ,Order (biology) ,Gene Expression Regulation ,Evolutionary biology ,010608 biotechnology ,Animals ,Functional significance ,Identification (biology) ,Gene ,Pseudogenes ,030304 developmental biology ,Biotechnology - Abstract
Pseudogenes, the debilitated parts of ancient genes, were previously scrapped off as junk or discarded genes with no functional significance. Pseudogenes have come under scrutiny for their functionality, since recent studies have unveiled their importance in the regulation of their corresponding parent genes and various biological mechanisms. Despite the enormous occurrence of pseudogenes in plants, the lack of experimental validation has contributed toward their unresolved roles in gene regulation. Contrarily, most of the studies associated with gene regulation have been mainly reported for humans, mice, and other mammalian genomes. Consequently, in order to present a cumulative report on plant-based pseudogenes research, an attempt has been made to assemble multiple studies presenting the pseudogene classification, the prediction and the determination of comparative accuracies of various computational pipelines, and recent trends in analyzing their biological functions, and regulatory mechanisms. This review represents the classical, as well as the recent advances on pseudogene identification and their potential roles in transcriptional regulation, which could possibly invigorate the quality of genome annotation, evolutionary analysis, and complexity surrounding the regulatory pathways in plants. Thus, when the ambiguous boundary girdling the pseudogenes eventually recedes on account of their explicit orchestration role, research in flora would no longer saunter compared to that on fauna.
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- 2021
25. The lncRNA PTTG3P promotes the progression of CRPC via upregulating PTTG1
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Ming Jia, Li Weibing, Meng Mingsen, Huang Shengquan, Deng Guoxian, Fang Qiang, Qianjin Liao, and Ji Huixiang
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Male ,0301 basic medicine ,Cancer Research ,Antineoplastic Agents, Hormonal ,Mice, Nude ,Adenocarcinoma ,Biology ,urologic and male genital diseases ,Binding, Competitive ,Tosyl Compounds ,Androgen deprivation therapy ,03 medical and health sciences ,Prostate cancer ,0302 clinical medicine ,Downregulation and upregulation ,Western blot ,Prostate ,Cell Line, Tumor ,Nitriles ,LNCaP ,medicine ,Animals ,Humans ,Anilides ,Radiology, Nuclear Medicine and imaging ,RNA, Messenger ,RNA, Neoplasm ,Tumor Stem Cell Assay ,medicine.diagnostic_test ,Competing endogenous RNA ,Androgen Antagonists ,Hematology ,General Medicine ,medicine.disease ,Up-Regulation ,Securin ,MicroRNAs ,Prostatic Neoplasms, Castration-Resistant ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Drug Resistance, Neoplasm ,Cell culture ,030220 oncology & carcinogenesis ,Cancer research ,Heterografts ,RNA, Long Noncoding ,Neoplasm Transplantation ,Pseudogenes - Abstract
Summary Background Overexpression of certain long non-coding RNAs (lncRNAs) promotes the progression of castration-resistant prostate cancer (CRPC). The significance and potential role of the lncRNA designated pituitary tumour-transforming 3, pseudogene (PTTG3P) in CRPC is unknown. Methods We detected PTTG3P expression by qPCR. Upregulated PTTG3P expression was performed to explore the role of PTTG3P in PCa cells resistant to ADT (androgen deprivation therapy). The relationship among PTTG3P, mir-146a-3p and PTTG1 were validated by qPCR, western blot and luciferase assay. Results PTTG3P levels were significantly increased in the androgen-independent PC cell lines, as well as in CRPC tissues compared with those of the androgen-dependent prostate cancer cell line LNCaP and tumour tissues of patients with hormone-naive prostate cancers. Enforced expression of PTTG3P in androgen-deprived LNCaP cells significantly enhanced survival, clonogenicity, and tumorigenicity. Further, PTTG3P acted as a competing endogenous RNA (ceRNA, natural miRNA sponge) to upregulate PTTG1 expression by competing for mir-146a-3p in the progression to CRPC. Conclusion Our findings suggest that PTTG3P promotes the resistance of prostate cancer cells to androgen-deprivation therapy via upregulating PTTG1. PTTG3P may therefore represent a potential target for therapy of CRPC.
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- 2021
26. Higher Rates of Processed Pseudogene Acquisition in Humans and Three Great Apes Revealed by Long-Read Assemblies
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Heng Li and Xiaowen Feng
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Pseudogene ,Gorilla ,Retrotransposon ,Genome ,Germline ,03 medical and health sciences ,Exon ,0302 clinical medicine ,biology.animal ,Genetics ,Animals ,Humans ,Molecular Biology ,Gene ,Discoveries ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,0303 health sciences ,Polymorphism, Genetic ,biology ,Hominidae ,Alternative Splicing ,Long Interspersed Nucleotide Elements ,Genetic Techniques ,Evolutionary biology ,Pseudogenes ,030217 neurology & neurosurgery ,Reference genome - Abstract
LINE-1-mediated retrotransposition of protein-coding mRNAs is an active process in modern humans for both germline and somatic genomes. Prior works that surveyed human data mostly relied on detecting discordant mappings of paired-end short reads, or exon junctions contained in short reads. Moreover, there have been few genome-wide comparisons between gene retrocopies in great apes and humans. In this study, we introduced a more sensitive and accurate method to identify processed pseudogenes. Our method utilizes long-read assemblies, and more importantly, is able to provide full-length retrocopy sequences as well as flanking regions which are missed by short-read based methods. From 22 human individuals, we pinpointed 40 processed pseudogenes that are not present in the human reference genome GRCh38 and identified 17 pseudogenes that are in GRCh38 but absent from some input individuals. This represents a significantly higher discovery rate than previous reports (39 pseudogenes not in the reference genome out of 939 individuals). We also provided an overview of lineage-specific retrocopies in chimpanzee, gorilla, and orangutan genomes.
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- 2021
27. Mining Proteomics Datasets to Uncover Functional Pseudogenes
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Anna, Meller and François-Michel, Boisvert
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Proteomics ,Proteome ,Genome, Human ,Humans ,Molecular Sequence Annotation ,Pseudogenes - Abstract
Analysis of the proteome, combined with the human genome sequence, improved gene annotation and confirmed that some genes are actually expressed as proteins, including pseudogenes, alternative reading frames, and additional protein isoforms. Although sequencing a genome is a challenge in itself, the annotation of all genes encoding proteins is a bigger one. Here, we describe an in silico approach to identify evidence of pseudogene expression, as well as an experimental approach for the validation of the protein encoded by pseudogenes, including the steps necessary to quantify these proteins. This technique enables a comprehensive analysis for the expression of genes into proteins that were not suspected of existing.
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- 2022
28. Pseudogene UBE2MP1 derived transcript enhances
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Fengjie, Hao, Nan, Wang, Honglian, Gui, Yifan, Zhang, Zhiyuan, Wu, and Junqing, Wang
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Gene Expression Regulation, Neoplastic ,MicroRNAs ,Carcinoma, Hepatocellular ,Liver Neoplasms ,Ubiquitin-Conjugating Enzymes ,Humans ,RNA, Long Noncoding ,Apoptosis ,Pseudogenes ,RGS Proteins ,Cell Proliferation ,Cell Line - Abstract
Pseudogenes are barely transcribed at normal, while the anomalous transcripts of them are mostly regarded as long non-coding RNAs (lncRNAs), which play potential functions in human tumorigenicity and development. The exact effects of pseudogene-derived transcripts on hepatocellular carcinoma (HCC) are ambiguous. According to our previous research and constructed database on the HCC-related lncRNAs, we noticed that UBE2MP1 was transcriptionally activated in HCC as a pseudogene from the ubiquitin-conjugating enzyme member UBE2M. In this study, we validated the high expression of the UBE2MP1 transcript in HCC and its adverse correlation with dismal outcomes for the patients. UBE2MP1 depletion at the transcript level significantly impaired cell proliferation and apoptosis resistance in HCC cell lines. Notably, we discovered that the UBE2MP1 transcript shared a specific sequence, binding to the miR-145-5p seed region with a typical ceRNA effect. Simultaneously, we verified an axis of miR-145-5p/RGS3 in HCC cells, which promoted cell proliferation and apoptosis resistance with significance. And modulation of UE2MP1 could remarkably affect RGS3 expression and consequentially influence HCC cell growth
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- 2022
29. ABCC6P1 pseudogene induces ABCC6 upregulation and multidrug resistance in breast cancer
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Mahnaz Hashemi and Masoud Golalipour
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Paclitaxel ,Breast Neoplasms ,General Medicine ,Drug Resistance, Multiple ,Up-Regulation ,Doxorubicin ,Drug Resistance, Neoplasm ,Cell Line, Tumor ,Genetics ,Humans ,Female ,Fluorouracil ,Cisplatin ,Multidrug Resistance-Associated Proteins ,Molecular Biology ,Pseudogenes - Abstract
The elevated drug efflux by ABC transports has been considered the primary mechanism of drug resistance in cancer. Recently, non-coding RNAs, such as pseudogenes, have been proposed to be involved in transporter-mediated drug resistance in cancer. The human genome has 22 ABC transporter pseudogenes. Among these pseudogenes, ABCC6P1 has co-expression with its ancestral gene in various human tissues. In the present study, we assessed the effect of ABCC6P1 pseudogene overexpression on ABCC6 expression and drug resistance.The ABCC6P1 was transfected into the MCF-7 and MDA-MB-231 cells. In ABCC6P1-overexpressing cells, the ABCC6 level significantly increased. The results of cell treatment with doxorubicin, 5-fluorouracil, cisplatin, and paclitaxel showed that the survival of ABCC6P1-overexpressing cells was higher than normal cells. Furthermore, uptake of doxorubicin was lower in ABCC6P1-overexpressing cells.In conclusion, our results show that overexpression of ABCC6P1 pseudogene induces the drug resistance phenotype, possibly through activation of the ancestral gene.
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- 2022
30. 'Pseudo-pseudogenes' in bacterial genomes: Proteogenomics reveals a wide but low protein expression of pseudogenes in Salmonella enterica
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Ye Feng, Zeyu Wang, Kun-Yi Chien, Hsiu-Ling Chen, Yi-Hua Liang, Xiaoting Hua, and Cheng-Hsun Chiu
- Subjects
Bacterial Proteins ,Salmonella paratyphi A ,Genetics ,Codon, Terminator ,Gene Expression ,Salmonella typhi ,Genome, Bacterial ,Pseudogenes ,Proteogenomics - Abstract
Pseudogenes (genes disrupted by frameshift or in-frame stop codons) are ubiquitously present in the bacterial genome and considered as nonfunctional fossil. Here, we used RNA-seq and mass-spectrometry technologies to measure the transcriptomes and proteomes of Salmonella enterica serovars Paratyphi A and Typhi. All pseudogenes’ mRNA sequences remained disrupted, and were present at comparable levels to their intact homologs. At the protein level, however, 101 out of 161 pseudogenes suggested successful translation, with their low expression regardless of growth conditions, genetic background and pseudogenization causes. The majority of frameshifting detected was compensatory for -1 frameshift mutations. Readthrough of in-frame stop codons primarily involved UAG; and cytosine was the most frequent base adjacent to the codon. Using a fluorescence reporter system, fifteen pseudogenes were confirmed to express successfully in vivo in Escherichia coli. Expression of the intact copy of the fifteen pseudogenes in S. Typhi affected bacterial pathogenesis as revealed in human macrophage and epithelial cell infection models. The above findings suggest the need to revisit the nonstandard translation mechanism as well as the biological role of pseudogenes in the bacterial genome.
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- 2022
31. Exploration of shared TF-miRNA‒mRNA and mRNA-RBP-pseudogene networks in type 2 diabetes mellitus and breast cancer
- Author
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Tong, Wu, Wenze, Gu, Libing, Hong, Yuchen, Cao, Hejia, Zhao, Xi, Guo, Xiongyi, Yang, Guoguo, Yi, and Min, Fu
- Subjects
Immunology ,RNA-Binding Proteins ,Breast Neoplasms ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Diabetes Mellitus, Type 2 ,Immunology and Allergy ,Cytochromes ,Humans ,Female ,Gene Regulatory Networks ,RNA, Messenger ,S-Phase Kinase-Associated Proteins ,Pseudogenes ,Transcription Factors - Abstract
Type 2 diabetes mellitus (T2DM) has been confirmed to be closely associated with breast cancer (BC). However, the shared mechanisms between these diseases remain unclear. By comparing different datasets, we identified shared differentially expressed (DE) RNAs in T2DM and BC, including 427 mRNAs and 6 miRNAs from the GEO(Gene Expression Omnibus) database. We used databases to predict interactions to construct two critical networks. The transcription factor (TF)-miRNA‒mRNA network contained 236 TFs, while the RNA binding protein (RBP)-pseudogene-mRNA network showed that the pseudogene S-phase kinase associated protein 1 pseudogene 1 (SKP1P1) might play a key role in regulating gene expression. The shared mRNAs between T2DM and BC were enriched in cytochrome (CYP) pathways, and further analysis of CPEB1 and COLEC12 expression in cell lines, single cells and other cancers showed that they were strongly correlated with the survival and prognosis of patients with BC. This result suggested that patients with T2DM presenting the downregulation of CPEB1 and COLEC12 might have a higher risk of developing BC. Overall, our work revealed that high expression of CYPs in patients with T2DM might be a susceptibility factor for BC and identified novel gene candidates and immune features that are promising targets for immunotherapy in patients with BC.
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- 2022
32. Mitochondrial Pseudogenes Suggest Repeated Inter-Species Hybridization among Direct Human Ancestors
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Konstantin Popadin, Konstantin Gunbin, Leonid Peshkin, Sofia Annis, Zoe Fleischmann, Melissa Franco, Yevgenya Kraytsberg, Natalya Markuzon, Rebecca R. Ackermann, Konstantin Khrapko, Department of Archaeology, and Faculty of Science
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natural hybridization ,mtdna ,human evolution ,hybridization ,mtDNA ,NUMT ,Hominidae ,adaptation ,primate ,complex speciation ,DNA, Mitochondrial ,diversity ,Mitochondria ,Evolution, Molecular ,numt ,evolution ,genetic-evidence ,origin ,Genetics ,admixture ,Animals ,Humans ,Hybridization, Genetic ,phylogenetic-relationships ,Genetics (clinical) ,Pseudogenes - Abstract
The hypothesis that the evolution of humans involves hybridization between diverged species has been actively debated in recent years. We present the following novel evidence in support of this hypothesis: the analysis of nuclear pseudogenes of mtDNA (“NUMTs”). NUMTs are considered “mtDNA fossils” as they preserve sequences of ancient mtDNA and thus carry unique information about ancestral populations. Our comparison of a NUMT sequence shared by humans, chimpanzees, and gorillas with their mtDNAs implies that, around the time of divergence between humans and chimpanzees, our evolutionary history involved the interbreeding of individuals whose mtDNA had diverged as much as ~4.5 Myr prior. This large divergence suggests a distant interspecies hybridization. Additionally, analysis of two other NUMTs suggests that such events occur repeatedly. Our findings suggest a complex pattern of speciation in primate/human ancestors and provide one potential explanation for the mosaic nature of fossil morphology found at the emergence of the hominin lineage. A preliminary version of this manuscript was uploaded to the preprint server BioRxiv in 2017 (10.1101/134502).
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- 2022
33. Not a piece of junk anymore: Pseudogene T04B2.1 performs non-conventional regulatory role and modulates aggregation of α- synuclein and β-amyloid proteins in C. elegans
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Aamir Nazir, Kottapalli Srividya, and Shikha Shukla
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0301 basic medicine ,Pseudogene ,Biophysics ,Piwi-interacting RNA ,Computational biology ,Biology ,Biochemistry ,Transcriptome ,03 medical and health sciences ,0302 clinical medicine ,RNA interference ,microRNA ,Animals ,Gene silencing ,Gene Silencing ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,Molecular Biology ,Gene knockdown ,Amyloid beta-Peptides ,Gene Expression Profiling ,Neurodegenerative Diseases ,Cell Biology ,Noncoding DNA ,Gene Ontology ,030104 developmental biology ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,alpha-Synuclein ,Pseudogenes - Abstract
The conventional notions of pseudogenes being ‘junk DNA’ have largely been offset as research studies have established their role in multiple biological processes. Our studies towards identification of genetic modulators employing C. elegans model, that associate reproductive health and age-related neurodegenerative diseases, led us to identification and functional characterization of a pseudogene T04B2.1, which when knocked down, exacerbates the aggregation of α-Synuclein and β-Amyloid proteins, induces lipid deposition and alters morphometric endpoints in worms. Whole transcriptome analysis of worms under knockdown condition of T04B2.1 revealed an altered expression of 187 sequences, most of these being non-coding RNAs, miRNAs and piRNAs modulating the RNAi regulatory processes. Our gene ontology and pathway enrichment analysis demonstrated the role of T04B2.1 in protein quality control, metabolic pathways and development. We further performed a signature motif search and successfully identified a common motif that is present between all piRNA and miRNA molecules, which are significantly altered upon T04B2.1 silencing. This study unveils the non-conventional regulatory role of pseudogene T04B2.1 with respect to effects associated with neurodegenerative diseases and encourages further studies to decipher the regulatory mechanism governed by pseudogenes.
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- 2021
34. TET3 dioxygenase modulates gene conversion at the avian immunoglobulin variable region via demethylation of non‐CpG sites in pseudogene templates
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Hidetaka Seo, Kunihiro Ohta, and Natsuki Takamura
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Pseudogene ,TET protein ,pseudogene ,Immunoglobulin Variable Region ,Somatic hypermutation ,Biology ,Cell Line ,Dioxygenases ,Cytosine ,03 medical and health sciences ,Cytidine Deaminase ,Genetics ,Animals ,Gene conversion ,Epigenetics ,Cell Proliferation ,030304 developmental biology ,0303 health sciences ,Genome ,gene conversion ,Original Articles ,Cell Biology ,Methylation ,Cytidine deaminase ,non‐CpG methylation ,DNA Methylation ,Cell biology ,DNA Demethylation ,DNA demethylation ,Gene Expression Regulation ,DNA methylation ,Original Article ,CpG Islands ,Immunoglobulin Light Chains ,immunoglobulin ,Chickens ,Pseudogenes - Abstract
Diversification of the avian primary immunoglobulin (Ig) repertoire is achieved in developing B cells by somatic hypermutation (SHM) and gene conversion (GCV). GCV is a type of homologous recombination that unidirectionally transfers segments of Ig pseudogenes to Ig variable domains. It is regulated by epigenetic mechanisms like histone modifications, but the role of DNA methylation remains unclear. Here, we demonstrate that the chicken B‐cell line DT40 lacking TET3, a member of the TET (Ten‐eleven translocation) family dioxygenases that facilitate DNA demethylation, exhibited a marked reduction in GCV activity in Ig variable regions. This was accompanied by a drop in the bulk levels of 5‐hydroxymethylcytosine, an oxidized derivative of 5‐methylcytosine, whereas TET1‐deficient or TET2‐deficient DT40 strains did not exhibit such effects. Deletion of TET3 caused little effects on the expression of proteins required for SHM and GCV, but induced hypermethylation in some Ig pseudogene templates. Notably, the enhanced methylation occurred preferably on non‐CpG cytosines. Disruption of both TET1 and TET3 significantly inhibited the expression of activation‐induced cytidine deaminase (AID), an essential player in Ig diversification. These results uncover unique roles of TET proteins in avian Ig diversification, highlighting the potential importance of TET3 in maintaining hypomethylation In Ig pseudogenes., We demonstrated the chicken B‐cell line DT40 lacking TET3, a member of the TET family proteins that facilitate DNA demethylation, exhibited a marked reduction in immunoglobulin (Ig) gene diversification. This was accompanied by enhanced methylation on non‐CpG cytosines in Ig pseudogene templates. These results uncover unique roles of TET3 in avian Ig diversification likely via maintaining hypomethylation in Ig pseudogenes.
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- 2021
35. Patterns and tempo of PCSK9 pseudogenizations suggest an ancient divergence in mammalian cholesterol homeostasis mechanisms
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Barbara van Asch and Luis Teixeira da Costa
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0106 biological sciences ,0301 basic medicine ,Gene loss ,Swine ,Lineage (evolution) ,Plant Science ,010603 evolutionary biology ,01 natural sciences ,PCSK9 ,Evolution, Molecular ,03 medical and health sciences ,Euarchontoglires ,Molecular evolution ,Genetics ,Cholesterol homeostasis ,Animals ,Humans ,Phylogeny ,Original Paper ,biology ,Eutheria ,Genetic Variation ,Human disease ,General Medicine ,biology.organism_classification ,Proprotein convertase ,Null allele ,Laurasiatheria ,Animal models ,Cholesterol ,030104 developmental biology ,Receptors, LDL ,Evolutionary biology ,Insect Science ,Kexin ,Animal Science and Zoology ,Proprotein Convertase 9 ,Pseudogenes - Abstract
Proprotein convertase subtilisin/kexin type 9 (PCSK9) plays a central role in cholesterol homeostasis in humans as a major regulator of LDLR levels. PCSK9 is an intriguing protease in that it does not act by proteolysis but by preventing LDLR recirculation from endosomes to the plasma membrane. This, and the inexistence of any other proteolytic substrate but itself could suggest that PCSK9 is an exquisite example of evolutionary fine-tuning. However, the gene has been lost in several mammalian species, and null alleles are present (albeit at low frequencies) in some human populations without apparently deleterious health effects, raising the possibility that the PCSK9 may have become dispensable in the mammalian lineage. To address this issue, we systematically recovered, assembled, corrected, annotated and analysed publicly available PCSK9 sequences for 420 eutherian species to determine the distribution, frequencies, mechanisms and timing of PCSK9 pseudogenization events, as well as the evolutionary pressures underlying the preservation or loss of the gene. We found a dramatic difference in the patterns of PCSK9 retention and loss between Euarchontoglires—where there is strong pressure for gene preservation—and Laurasiatheria, where multiple independent events have led to PCSK9 loss in most species. These results suggest that there is a fundamental difference in the regulation of cholesterol metabolism between Euarchontoglires and Laurasiatheria, which in turn has important implications for the use of Laurasiatheria species (e.g. pigs) as animal models of human cholesterol-related diseases.
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- 2021
36. Behind the Scene: Surprises and Snags of Pseudogenes
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Mario J. Simirgiotis, Tanveer Majeed, Musarrat Abbas Khan, Hafiz Ishfaq Ahmad, M Mahnoor, Hebatallah A. Nasser, Abdul Jabbar, Shakeel Ahmed, Muhammad Imran, and Mahmoud M. Tolba
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Pseudogene ,RNA ,Tumor therapy ,Genomics ,Genetic Therapy ,Computational biology ,Biology ,Biological Evolution ,Noncoding DNA ,Genetic therapy ,Neoplasms ,microRNA ,Genetics ,Humans ,RNA Interference ,RNA, Small Interfering ,Molecular Biology ,Gene ,Pseudogenes - Abstract
The junk DNA "pseudogenes," known as genomic fossils, are characterized by their ubiquitousness and abundance within the genomic structure. These genomics sets are recognized by the potential activity of meta-regulating the parent genes; these are transcribed into interfering RNA, consequently acting on miRNA concentration, thereby shedding light on the crosstalk of the pseudogenes' miRNA, siRNA, lncRNA/tumor therapy co-relationship. Moreover, an upcoming visualization regarding pseudogenes is under investigation, which describes the potentiality of pseudogenes as a fundamental component of cancerous evolutionary processing tools. Accordingly, here is a systematic review covering pseudobirth, pseudosignatures, and functional properties of pseudogenes, concluding that these pseudogenes are hypothetically predictive tumor therapies.
- Published
- 2021
37. Stepwise evolution of Salmonella Typhimurium ST313 causing bloodstream infection in Africa
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Rebecca J. Bennett, Melita A. Gordon, Rebecca J. Bengtsson, Mike P. Mautanga, Neil Hall, Jessica A. Bevington, Xiaojun Zhu, Alexandra Beliavskaia, P. Malaka De Silva, Arthur Bowers-Barnard, Kate S. Baker, Benjamin Kumwenda, Jay C. D. Hinton, Hermione J. Webster, François-Xavier Weill, George E. Stenhouse, Blanca M. Perez-Sepulveda, Happy C. Banda, Nicholas A. Feasey, Will P. M. Rowe, Reenesh Prakash, Ella V. Rodwell, Alexander V. Predeus, Caisey V. Pulford, Nicolas Wenner, Amy Dumigan, José A. Bengoechea, Alistair C. Darby, Rocío Canals, Alicia Tran-Dien, and Lovemore Alufandika
- Subjects
Salmonella typhimurium ,Salmonella ,Lineage (evolution) ,Bacteremia ,Genome informatics ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Genome ,Genotype ,Phylogeny ,Genetics ,0303 health sciences ,Virulence ,Phylogenetic tree ,Phylogenetics ,Phenotype ,Salmonella enterica ,Salmonella Infections ,Pseudogenes ,Plasmids ,Microbiology (medical) ,Immunology ,Biology ,Microbiology ,Article ,Evolution, Molecular ,03 medical and health sciences ,Antibiotic resistance ,SDG 3 - Good Health and Well-being ,Sepsis ,Drug Resistance, Bacterial ,medicine ,Humans ,030304 developmental biology ,030306 microbiology ,Genetic Variation ,Cell Biology ,biology.organism_classification ,Computational biology and bioinformatics ,Africa ,Molecular evolution ,Genome, Bacterial - Abstract
Bloodstream infections caused by nontyphoidal Salmonella are a major public health concern in Africa, causing ~49,600 deaths every year. The most common Salmonella enterica pathovariant associated with invasive nontyphoidal Salmonella disease is Salmonella Typhimurium sequence type (ST)313. It has been proposed that antimicrobial resistance and genome degradation has contributed to the success of ST313 lineages in Africa, but the evolutionary trajectory of such changes was unclear. Here, to define the evolutionary dynamics of ST313, we sub-sampled from two comprehensive collections of Salmonella isolates from African patients with bloodstream infections, spanning 1966 to 2018. The resulting 680 genome sequences led to the discovery of a pan-susceptible ST313 lineage (ST313 L3), which emerged in Malawi in 2016 and is closely related to ST313 variants that cause gastrointestinal disease in the United Kingdom and Brazil. Genomic analysis revealed degradation events in important virulence genes in ST313 L3, which had not occurred in other ST313 lineages. Despite arising only recently in the clinic, ST313 L3 is a phylogenetic intermediate between ST313 L1 and L2, with a characteristic accessory genome. Our in-depth genotypic and phenotypic characterization identifies the crucial loss-of-function genetic events that occurred during the stepwise evolution of invasive S. Typhimurium across Africa., Stepwise evolution of invasive Salmonella Typhimurium in Africa is defined using genotypic and phenotypic analyses of isolates collected over a 50-yr period.
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- 2020
38. GENCODE 2021
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Fabio C. P. Navarro, Jonathan M. Mudge, S. Mohanan, Adam Frankish, Joel Armstrong, Tiago Grego, Irwin Jungreis, Roderic Guigó, Jinrui Xu, Benedict Paten, Cristina Sisu, Daniel R. Zerbino, Julien Lagarde, Mark Diekhans, José M. González, Michael L. Tress, E. Stapleton, Osagie G. Izuogu, Mark Gerstein, Ian T. Fiddes, Toby Hunt, Sarah Donaldson, Marie Marthe Suner, Fernando Pozo, Andrew D. Yates, S. Carbonell Sala, T. Di Domenico, Matthew Hardy, Barbara Uszczynska-Ratajczak, Fiona Cunningham, Andrew Berry, Anne Parker, Laura Martinez, Alexandra Bignell, Bianca M. Schmitt, Yan Zhang, Jane E. Loveland, Baikang Pei, Jyoti S. Choudhary, F. C. Riera, Paul R. Muir, C. Garcia Giron, Tim Hubbard, Fergal J. Martin, Rory Johnson, Magali Ruffier, If Barnes, James C. Wright, I. Sycheva, Manolis Kellis, Carles Boix, Thibaut Hourlier, Paul Flicek, Maxim Y Wolf, Y. T. Yang, and Kerstin Howe
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Transcription, Genetic ,AcademicSubjects/SCI00010 ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,610 Medicine & health ,Computational biology ,Genome browser ,Biology ,Genome ,Mice ,03 medical and health sciences ,Annotation ,0302 clinical medicine ,Databases, Genetic ,Genetics ,Database Issue ,Animals ,Humans ,Ensembl ,Epidemics ,GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries) ,030304 developmental biology ,Internet ,0303 health sciences ,SARS-CoV-2 ,GENCODE ,COVID-19 ,Computational Biology ,Molecular Sequence Annotation ,Genomics ,ComputingMethodologies_PATTERNRECOGNITION ,Genome Biology ,RNA, Long Noncoding ,Pseudogenes ,030217 neurology & neurosurgery ,Reference genome - Abstract
© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. The GENCODE project annotates human and mouse genes and transcripts supported by experimental data with high accuracy, providing a foundational resource that supports genome biology and clinical genomics. GENCODE annotation processes make use of primary data and bioinformatic tools and analysis generated both within the consortium and externally to support the creation of transcript structures and the determination of their function. Here, we present improvements to our annotation infrastructure, bioinformatics tools, and analysis, and the advances they support in the annotation of the human and mouse genomes including: the completion of first pass manual annotation for the mouse reference genome; targeted improvements to the annotation of genes associated with SARS-CoV-2 infection; collaborative projects to achieve convergence across reference annotation databases for the annotation of human and mouse protein-coding genes; and the first GENCODE manually supervised automated annotation of lncRNAs. Our annotation is accessible via Ensembl, the UCSC Genome Browser and https://www.gencodegenes.org. National Human Genome Research Institute of the National Institutes of Health [U41HG007234]; the content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health; Wellcome Trust [WT108749/Z/15/Z, WT200990/Z/16/Z]; European Molecular Biology Laboratory; Swiss National Science Foundation through the National Center of Competence in Research ‘RNA & Disease’ (to R.J.); Medical Faculty of the University of Bern (to R.J). Funding for open access charge: National Institutes of Health.
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- 2020
39. Multiple origins of obligate nematode and insect symbionts by a clade of bacteria closely related to plant pathogens
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Brent E. Gowen, John Jaenike, Vincent G. Martinson, Ryan M.R. Gawryluk, Caitlin I. Curtis, and Steve J. Perlman
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Sodalis ,food.ingredient ,media_common.quotation_subject ,Pectobacterium ,Insect ,Biology ,Rhabditida ,03 medical and health sciences ,food ,Enterobacteriaceae ,Symbiosis ,Animals ,Clade ,Phylogeny ,030304 developmental biology ,media_common ,0303 health sciences ,Multidisciplinary ,Obligate ,030306 microbiology ,Ecology ,fungi ,Genomics ,Biological Sciences ,biology.organism_classification ,Nematode ,Candidatus ,Drosophila ,Genome, Bacterial ,Pseudogenes ,Bacteria - Abstract
Obligate symbioses involving intracellular bacteria have transformed eukaryotic life, from providing aerobic respiration and photosynthesis to enabling colonization of previously inaccessible niches, such as feeding on xylem and phloem, and surviving in deep-sea hydrothermal vents. A major challenge in the study of obligate symbioses is to understand how they arise. Because the best studied obligate symbioses are ancient, it is especially challenging to identify early or intermediate stages. Here we report the discovery of a nascent obligate symbiosis in Howardula aoronymphium, a well-studied nematode parasite of Drosophila flies. We have found that H. aoronymphium and its sister species harbor a maternally inherited intracellular bacterial symbiont. We never find the symbiont in nematode-free flies, and virtually all nematodes in the field and the laboratory are infected. Treating nematodes with antibiotics causes a severe reduction in fly infection success. The association is recent, as more distantly related insect-parasitic tylenchid nematodes do not host these endosymbionts. We also report that the Howardula nematode symbiont is a member of a widespread monophyletic group of invertebrate host-associated microbes that has independently given rise to at least four obligate symbioses, one in nematodes and three in insects, and that is sister to Pectobacterium, a lineage of plant pathogenic bacteria. Comparative genomic analysis of this group, which we name Candidatus Symbiopectobacterium, shows signatures of genome erosion characteristic of early stages of symbiosis, with the Howardula symbiont’s genome containing over a thousand predicted pseudogenes, comprising a third of its genome.
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- 2020
40. The LGMN pseudogene promotes tumor progression by acting as a miR-495-3p sponge in glioblastoma
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Xiaojie Lu, Shuai Zhang, Renhao Yang, Renhua Huang, Jin Lan, Zhiqiang Li, Xiwen Song, Binghong Chen, Yingying Lin, Lilin Cheng, Keman Liao, Tianwei Wang, Zhongrun Qian, Yongming Qiu, and Lin Kong
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0301 basic medicine ,Cancer Research ,Pseudogene ,Mice, Nude ,Legumain ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,microRNA ,Animals ,Humans ,Gene silencing ,biology ,Brain Neoplasms ,Competing endogenous RNA ,RNA ,Gene Expression Regulation, Neoplastic ,Cysteine Endopeptidases ,MicroRNAs ,030104 developmental biology ,Oncology ,Tumor progression ,030220 oncology & carcinogenesis ,Disease Progression ,biology.protein ,Cancer research ,Heterografts ,RNA, Long Noncoding ,Glioblastoma ,Pseudogenes - Abstract
Pseudogenes, which are long noncoding RNAs that originate from protein-coding genes, have been suggested to play important roles in disease. Although studies have revealed high expression of legumain (LGMN) in many types of tumors, the regulation of LGMN remains largely unknown. Here, we found that a novel LGMN pseudogene (LGMNP1) was upregulated in glioblastoma (GBM) tissues and high LGMNP1 expression in GBM cells enhanced proliferation and invasion. Biochemical analysis showed that cytoplasmic LGMNP1 functionally targeted miR-495-3p in a manner involving an RNA-induced silencing complex. Dual-luciferase reporter assays demonstrated that LGMN was a target of miR-495-3p, and LGMN was upregulated and positively correlated with LGMNP1 in GBM. Moreover, miR-495-3p was downregulated and negatively correlated with LGMNP1 in GBM tissues. Notably, the tumor-promoting effects of LGMNP1 upregulation could be alleviated by miR-495-3p mimics. Furthermore, GBM cells overexpressing LGMNP1 exhibited more aggressive tumor progression and elevated LGMN expression in vivo. Thus, our data illustrate that LGMNP1 exerts its oncogenic activity, at least in part, as a competitive endogenous RNA (ceRNA) that elevates LGMN expression by sponging miR-495-3p. CeRNA-mediated miRNA sequestration might be a novel therapeutic strategy in GBM.
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- 2020
41. lncRNA TPTEP1 inhibits stemness and radioresistance of glioma through miR‑106a‑5p‑mediated P38 MAPK signaling
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Rong‑Mou Zhang, Mei‑Li Yang, Ting Tang, and Ling‑Xing Wang
- Subjects
0301 basic medicine ,Cancer Research ,Cell ,Apoptosis ,Radiation Tolerance ,p38 Mitogen-Activated Protein Kinases ,Biochemistry ,MAPK14 ,0302 clinical medicine ,glioma ,Brain Neoplasms ,Articles ,Cell cycle ,Prognosis ,Gene Expression Regulation, Neoplastic ,microRNA-106a-5p ,radioresistance ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Disease Progression ,Neoplastic Stem Cells ,Molecular Medicine ,RNA, Long Noncoding ,Pseudogenes ,Signal Transduction ,MAP Kinase Signaling System ,Biology ,stemness ,03 medical and health sciences ,Cancer stem cell ,Cell Line, Tumor ,Glioma ,Radioresistance ,microRNA ,Genetics ,medicine ,Humans ,neoplasms ,Molecular Biology ,Cell Proliferation ,Oncogene ,PTEN Phosphohydrolase ,Membrane Proteins ,medicine.disease ,Molecular medicine ,nervous system diseases ,MicroRNAs ,transmembrane phosphatase with tensin homology pseudogene 1 ,030104 developmental biology ,Cancer research ,Neoplasm Recurrence, Local - Abstract
Glioma is diagnosed as the most common intracranial malignant tumor. Cancer stem cells determine stemness and radioresistance, and may facilitate glioma recurrence. The present study aimed to investigate whether the long non-coding RNA (lncRNA) transmembrane phosphatase with tensin homology pseudogene 1 (TPTEP1) regulated cell stemness and radioresistance of glioma, and determine the underlying molecular mechanism of TPTEP1 in the modulation of glioma progression. Cell and molecular biology techniques were applied for investigating the role of TPTEP1 in glioma cell lines, animal model, and clinical samples. The results demonstrated that TPTEP1 attenuated stemness and radioresistance of glioma both in vitro and in vivo. In addition, TPTEP1 augmented MAPK14 expression by competitively interacting with microRNA (miR)-106a-5p, thus activating the P38 MAPK signaling pathway, and suppressing glioma stemness and radioresistance. TPTEP1 functionally bound to miR-106a-5p, which formed a reciprocal regulatory loop to stimulate the P38 MAPK signaling pathway. Low TPTEP1 expression levels were detected in high-grade glioma tissues compared with low-grade glioma tissues, and were positively associated with poor prognosis of patients with glioma. Furthermore, analysis using data from The Cancer Genome Atlas database confirmed the molecular mechanism and biological significance of dysregulation of TPTEP1 in glioma progression. Taken together, the results of the present study suggest that TPTEP1 may be applied as a diagnostic and prognostic indicator for glioma, and may be an alternative target for the treatment of glioma.
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- 2020
42. The development and controversy of competitive endogenous RNA hypothesis in non-coding genes
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Hongcheng Liu, Yuchen Wei, Weimin Lin, Yonghang Tang, Lifan Zhang, Jie Chen, and Wei Wei
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0301 basic medicine ,Pseudogene ,Clinical Biochemistry ,Regulator ,Apoptosis ,Computational biology ,Biology ,Epigenesis, Genetic ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Species Specificity ,Neoplasms ,microRNA ,Animals ,Humans ,Gene Regulatory Networks ,Epigenetics ,Caenorhabditis elegans ,3' Untranslated Regions ,Molecular Biology ,Gene ,Models, Genetic ,Competing endogenous RNA ,Mechanism (biology) ,RNA ,RNA, Circular ,Cell Biology ,General Medicine ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,030220 oncology & carcinogenesis ,RNA, Long Noncoding ,Transcriptome ,Pseudogenes - Abstract
As a momentous post-transcriptional regulator, microRNAs (miRNAs) are attracting more and more attention. The classical miRNAs regulated mechanism shows it binds to the targets' 3'UTR thus play the role in post-transcription. Meanwhile, single miRNA can target multiple genes, so those should compete to bind that miRNA. Vice versa, single gene can sponge mass of miRNAs as well. Thus the competitive endogenous RNAs (ceRNAs) hypothesis was put forward in 2011. The ceRNA hypothesis has made huge achievements, in particular in non-coding genes, which including long non-coding RNAs (lncRNAs), circle RNAs (circRNAs) and pseudogenes, even viral transcripts. It also contributed greatly to epigenetics development. However, an increasing number of controversies have occurred with applause. Based on this situation, this review introduces something in detail about the ceRNAs hypothesis achieved in lncRNAs, circRNAs, pseudogenes and viral transcripts, respectively. Meanwhile, it also covers controversy of the ceRNAs hypothesis.
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- 2020
43. Resolving misalignment interference for NGS-based clinical diagnostics
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Hanlin Gao, Alan W Zhong, Che-Yu Lee, and Hai-Yun Yen
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0303 health sciences ,education.field_of_study ,Pseudogene ,030305 genetics & heredity ,Population ,Genetic Diseases, Inborn ,High-Throughput Nucleotide Sequencing ,Read depth ,Computational biology ,Biology ,Polymerase Chain Reaction ,Polymorphism, Single Nucleotide ,Sequence identity ,03 medical and health sciences ,Human disease ,Genetics ,Humans ,Multiplex ligation-dependent probe amplification ,Carrier screening ,education ,Algorithms ,Pseudogenes ,Genetics (clinical) ,Analysis method ,030304 developmental biology - Abstract
Next-generation sequencing (NGS) is an incredibly useful tool for genetic disease diagnosis. However, the most commonly used bioinformatics methods for analyzing sequence reads insufficiently discriminate genomic regions with extensive sequence identity, such as gene families and pseudogenes, complicating diagnostics. This problem has been recognized for specific genes, including many involved in human disease, and diagnostic labs must perform additional costly steps to guarantee accurate diagnosis in these cases. Here we report a new data analysis method based on the comparison of read depth between highly homologous regions to identify misalignment. Analyzing six clinically important genes-CYP21A2, GBA, HBA1/2, PMS2, and SMN1-each exhibiting misalignment issues related to homology, we show that our technique can correctly identify potential misalignment events and be used to make appropriate calls. Combined with long-range PCR and/or MLPA orthogonal testing, our clinical laboratory can improve variant calling with minimal additional cost. We propose an accurate and cost-efficient NGS testing procedure that will benefit disease diagnostics, carrier screening, and research-based population studies.
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- 2020
44. Identification of Candidate lncRNA and Pseudogene Biomarkers Associated with Carbon-Nanotube-Induced Malignant Transformation of Lung Cells and Prediction of Potential Preventive Drugs
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Guangtao Chang, Dongli Xie, Jianchen Hu, Tong Wu, Kangli Cao, and Xiaogang Luo
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Gene Expression Regulation, Neoplastic ,MicroRNAs ,Lung Neoplasms ,carbon nanotubes ,malignant transformation ,lung cancer ,non-coding RNAs ,Nanotubes, Carbon ,Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,Humans ,Nuclear Proteins ,RNA, Long Noncoding ,Lung ,Biomarkers ,Pseudogenes - Abstract
Mounting evidence has linked carbon nanotube (CNT) exposure with malignant transformation of lungs. Long non-coding RNAs (lncRNAs) and pseudogenes are important regulators to mediate the pathogenesis of diseases, representing potential biomarkers for surveillance of lung carcinogenesis in workers exposed to CNTs and possible targets to develop preventive strategies. The aim of this study was to screen crucial lncRNAs and pseudogenes and predict preventive drugs. GSE41178 (small airway epithelial cells exposed to single- or multi-walled CNTs or dispersant control) and GSE56104 (lung epithelial cells exposed to single-walled CNTs or dispersant control) datasets were downloaded from the Gene Expression Omnibus database. Weighted correlation network analysis was performed for these two datasets, and the turquoise module was preserved and associated with CNT-induced malignant phenotypes. In total, 24 lncRNAs and 112 pseudogenes in this module were identified as differentially expressed in CNT-exposed cells compared with controls. Four lncRNAs (MEG3, ARHGAP5-AS1, LINC00174 and PVT1) and five pseudogenes (MT1JP, MT1L, RPL23AP64, ZNF826P and TMEM198B) were predicted to function by competing endogenous RNA (MEG3/RPL23AP64-hsa-miR-942-5p-CPEB2/PHF21A/BAMBI; ZNF826P-hsa-miR-23a-3p-SYNGAP1, TMEM198B-hsa-miR-15b-5p-SYNGAP1/CLU; PVT1-hsa-miR-423-5p-PSME3) or co-expression (MEG3/MT1L/ZNF826P/MT1JP-ATM; ARHGAP5-AS1-TMED10, LINC00174-NEDD4L, ARHGAP5-AS1/PVT1-NIP7; MT1L/MT1JP-SYNGAP1; MT1L/MT1JP-CLU) mechanisms. The expression levels and prognosis of all genes in the above interaction pairs were validated using lung cancer patient samples. The receiver operating characteristic curve analysis showed the combination of four lncRNAs, five pseudogenes or lncRNAs + pseudogenes were all effective for predicting lung cancer (accuracy >0.8). The comparative toxicogenomics database suggested schizandrin A, folic acid, zinc or gamma-linolenic acid may be preventive drugs by reversing the expression levels of lncRNAs or pseudogenes. In conclusion, this study highlights lncRNAs and pseudogenes as candidate diagnostic biomarkers and drug targets for CNT-induced lung cancer.
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- 2022
45. Gene Flow Patterns of the Aedes aegypti (Diptera: Culicidae) Mosquito in Colombia: a Continental Comparison Suggests Multiple Invasion Routes and Gene Exchange
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Bryan Steven Valencia-Marín, Oscar Alexander Aguirre-Obando, and Mário Antônio Navarro-Silva
- Subjects
Arbovirus ,General Engineering ,mitochondrial DNA ,Americas ,Mosquitoes ,Pseudogenes - Abstract
In Colombia Aedes aegypti is present in 80% of the country up to 2,300 m; however, little is known of its genetic relations within a country context and, hence, within a broader context, for example, America. The aforementioned, herein, analyzed the gene flow within a context of the Americas, its directionality and genetic diversity of the mitochondrial lineages in the A. aegypti populations for Colombia. This called for the use of the sequences for A. aegypti available of the mitochondrial ND4 gene in the GenBank for Colombia and the American continent. No presence was found of nuclear mitochondrial pseudogenes (NUMTs) for Colombia. It is estimated that in Colombia the gene flow of the A. aegypti populations is occurring from the southeast and northeast toward the center of the country. In comparison with the mitochondrial sequences for America, the vector’s haplotypes in Colombia suggest connections between the populations of mosquitoes from the south with those from the north of the continent. The gene flow model at continental scale suggests bidirectional connections between the populations from the north of the continent with those from the south, while at South American scale it proposes the gene flow in all the directions with respect to the Colombian. The Colombian A. aegypti vector monitoring and control strategies must pay special attention to the vector’s points of entry into Colombia related with Peru, Venezuela, Brazil, Mexico, and North America to avoid the entry of populations with characteristics like resistance to insecticides or vector competition.
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- 2022
46. Global phylogeography of the flood mosquito, Aedes vexans (Diptera: Culicidae), from mitochondrial DNA
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José Heriberto Vargas-Espinosa and Oscar Alexander Aguirre-Obando
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Cytochrome oxidase subunit I ,genetic structuring ,haplotypes ,pseudogenes ,vector control ,Animal Science and Zoology ,genetic diversity ,mitochondrial DNA ,gene flow - Abstract
This contribution endeavored to investigate the genetic structure and gene flow of the flood mosquito, Aedes vexans (Meigen, 1830). Using partial sequences of the mitochondrial COI gene, available from BOLD Systems and GenBank, the Haplotypic (Hd) and nucleotide (π) gene diversity, genetic structuring and gene flow of A. vexans at the global, continental, and country levels were calculated. In total, 1,184 sequences were obtained, distributed among America (88.60%; represented by EUA and Canada), Europe (7.35%), Asia (3.89%), and Africa (0.17%). From these, 395 haplotypes (H) without presence of pseudogenes (NUMTs) were detected. The cluster analyses grouped the haplotypes into six clades. Clade I includes haplotypes from countries in America and Europe, while clades II and III include haplotypes exclusively from Asia and Europe; clade IV grouped only one haplotype from Africa and clade V grouped haplotypes from America and Africa. The global Hd and π were 0.92 and 0.01, respectively. In addition, there is evidence of genetic structuring among continents (7.07%), countries (1.62%), and within countries (91.30%; FST = 0.08, p < 0.05) and no isolation by distance was detected (r = 0.003, p > 0.05). The genetic diversity of A. vexans was found to be greater in North America than in other continents. Although this provisional conclusion might be influenced by a sample bias, since 88.60% of the sequences are from America, is also plausible to consider that America corresponds to the ancestral distribution area of the flood mosquito. This hypothesis needs further testing, using a more comprehensive sample from other continents. Additionally, the six clusters found and their geographical distribution do not support previous proposals of splitting the genus into three subspecies confined to certain geographical areas.
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- 2022
47. Nuclear ribosomal DNA secondary structures and statistical approach for the phylogeny of Ampelomyces
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Prahl, Rosa
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Computational ecology and phylogenetics ,Ampelomyces ,Ampelomyces humuli ,biocontrol ,environmental DNA ,evolution ,ITS1 and ITS2 secondary structures ,phylogeny ,plant protection ,powdery mildews ,pseudogenes ,ribosomal biogenesis - Abstract
Fungi belonging to the genus Ampelomyces are intracellular mycoparasites of the Erysiphales, which cause powdery mildew diseases in economically important crops. As Ampelomyces spp. are parasites of other parasites, they are named hyperparasites. Several Ampelomyces-based biocontrol agents have been developed but its effectivity against different powdery mildew fungi can vary, causing to still rely on the use of harmful pesticides. Accurate identification of these hyperparasites is essential to discovery newly highly virulent isolates for using in plant protection. In addition, further research is needed in Ampelomyces to understand, for example, their underlying mechanisms of virulence. One of the problems in studying Ampelomyces is their unresolved taxonomy, which has been mainly determined by phylogenetic analyses based on the nuclear ribosomal DNA internal transcribed spacer region (nrDNA ITS). The ITS region of the 18S-28S nuclear ribosomal DNA is the preferred fungal barcode and it consists of the ITS spacers 1 (ITS1) and 2 (ITS2), and the 5.8S gene. Historically, Ampelomyces were confused with other closely related pycnidial fungi. Today, many nucleotide sequences are deposited in the GenBank database under the generic name of Ampelomyces, but it is unknown if these sequences belong to the type specimen of Ampelomyces, Ampelomyces quisqualis Ces.. As the hyperparasites asexual structures vary in dimensions and apparently are not linked to a particular mycoparasitic lineage, their identification rely on the use of molecular barcodes. At least four distinct lineages have been identified based on the ITS sequences and actin 1 gene fragments (actin1). These DNA markers are insufficient for species delimitation in this genus, and thus, other identifiers are sought to assist in this purpose. This PhD thesis presents a comprehensive research and analysis conducted to optimise Ampelomyces identification. As the early works are showing the ITS spacers and secondary structures (S2s) are important molecular tools in phylogenetic studies. This PhD thesis has conducted an in-depth evaluation of the utility of ITS S2s in Ampelomyces phylogeny. The results of this thesis showed that: 1) putative Ampelomyces ITS sequences derived from environmental DNA (eDNA) and deposited in the GenBank do not belong to this genus. Statistical tests revealed that there were significant differences in the sequence lengths of ITS region as well as A/T nucleotide content values between the 'true' Ampelomyces spp. and those from putative Ampelomyces; 2) nrDNA ITS S2s significantly improves likelihood-based estimates of phylogeny; 3) the statistical test, Kruskal-Wallis, supports that the ITS spacers are under functional constraints of their S2s and indicating are not evolving neutrally as usually assumed for the nrDNA gene repeats; 4) variations in the conserved ITS2proximal stem of two Ampelomyces ITS sequences reveals the presence of pseudogenes; 5) the simultaneous alignment of ITS spacers and S2s reflects the Ampelomyces phylogeny and reveals strong phylogenetic signal in structure variation across lineages; and 6) DNA repeats of Ampelomyces ITS1 sequences were different from those of putative Ampelomyces. Altogether, these findings are important for resolving the taxonomic classification of Ampelomyces and understanding their evolutionary history. This computational work has also resolved (1) the previous difficulties in selecting a new DNA barcode with more phylogenetic resolution power than that of the ITS by using nrDNA ITS sequences and S2s based phylogeny and (2) some of the problems associated with selecting the 'true' Ampelomyces sequences without collecting and sequencing all previous ITS sequences deposited in the GenBank database. These findings will lead to accurately identify fungi in the future, improve DNA sequence data in databases as well as resolve the misidentification of Ampelomyces spp. sensu stricto.
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- 2022
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48. The double homeobox a pseudogene 8 accelerates cell proliferation, migration, and invasion in colon cancer
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Mao, Shengxun, Mo, Zhaohong, Wu, Runxin, Lai, Bin, Zhou, Zhiyong, Song, Yi, Ouyang, Xi, and Zhu, Xingen
- Subjects
Genes, Homeobox ,Bioengineering ,General Medicine ,Applied Microbiology and Biotechnology ,Gene Expression Regulation, Neoplastic ,Mice ,MicroRNAs ,Cell Movement ,Cell Line, Tumor ,Colonic Neoplasms ,Animals ,Humans ,RNA, Long Noncoding ,Pseudogenes ,Cell Proliferation ,Biotechnology - Abstract
Double homeobox A pseudogene 8 (DUXAP8) is a known tumor promoter in several malignancies. Nonetheless, its function in colon cancer (CC) is indefinite. Herein, we explored the significance of DUXAP8 and its underlying mechanism in CC. Our data indicated that DUXAP8 was upregulated in CC, and it was related to advanced stages and lymph node metastases. Based on our Kaplan-Meier survival analysis, elevated DUXAP8 expression resulted in shorter patient overall survival (OS). Conversely, DUXAP8 silencing strongly suppressed cellular proliferation, migration and invasion in vitro. Based on our western blot analysis, DUXAP8 deficiency strongly inhibited the epithelial���mesenchymal transition (EMT) in vitro. Alternately, DUXAP8 overexpression accelerated cellular proliferation migration and invasion in CC. Finally, silencing DUXAP8 prevented tumorigenesis in a mouse xenograft model in vivo. Collectively, our results demonstrated that DUXAP8 regulates the occurrence and advancement of CC, and may serve as a regulatory hub for this disease.
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- 2022
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49. Identification of processed pseudogenes in the genome of Thoroughbred horses: Possibility of gene-doping detection considering the presence of pseudogenes
- Author
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Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei‐ichi Hirota, Kanichi Kusano, and Shun‐ichi Nagata
- Subjects
Doping in Sports ,Genome ,Genetics ,Animals ,Animal Science and Zoology ,General Medicine ,Horses ,Introns ,Pseudogenes ,DNA Primers - Abstract
Processed pseudogenes, also known as retrocopy genes, are copies of messenger RNAs that have been reverse transcribed into DNA and inserted into the genome. In this study, we identified 62 processed pseudogene candidates as intron-less genes from whole-genome sequencing (WGS) data of Thoroughbred horses using delly structural variation software. The 62 processed pseudogene candidates were confirmed by PCR amplification of intron-less products. A total of 11 processed pseudogenes were confirmed in the genome of all 23 analysed horses, whereas three processed pseudogenes with structures of ATP11B, DPH3 and RPL17 were detected in only one of 115 horses by PCR amplification of intron-less products. Currently, most of the gene doping tests proposed in human and horse sports are adapted PCR-based methods using hydrolysis probes to detect exon/exon junctions in transgenes because the operation is simple and economical. However, when the pseudogene is present in the host genome, the PCR-based methods may have a potential risk of detecting false positives. In this study, because processed pseudogenes that exist less frequently in the horse genome may affect PCR-based transgene detection in gene-doping tests, we propose and demonstrate that PCR amplification and sequencing using primers designed on transgene and promotors and/or polyadenylation signal for gene expression are useful for gene-doping detection as an additional confirmatory test to prevent false positives.
- Published
- 2021
50. Characterization of HMGA1P6 transgenic mouse embryonic fibroblasts
- Author
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Marco De Martino, Paolo Chieffi, Francesco Esposito, Claudio Arra, Giuseppe De Palma, Alfredo Fusco, De Martino, M., Palma, G., Arra, C., Chieffi, P., Fusco, A., and Esposito, F.
- Subjects
0301 basic medicine ,Senescence ,Genetically modified mouse ,HMGA1 ,senescence ,Pseudogene ,pseudogene ,HMGA1P6 ,03 medical and health sciences ,0302 clinical medicine ,CeRNA ,medicine ,Molecular Biology ,pseudogenes ,biology ,Competing endogenous RNA ,Cancer ,Cell Biology ,medicine.disease ,Embryonic stem cell ,Cell biology ,030104 developmental biology ,030220 oncology & carcinogenesis ,biology.protein ,Developmental Biology - Abstract
Latest studies have shown that deregulated pseudogene transcripts contribute to cancer working as competing endogenous RNAs. Our research group has recently demonstrated that the overexpression of two HMGA1 pseudogenes, HMGA1P6 and HMGA1P7, has a critical role in cancer progression. These pseudogenes work sustaining the expression of HMGA1 and other cancer-related genes. We generated a mouse model overexpressing HMGA1P6 to better study the HMGA1-pseudogene function in a more physiological context. Here, we show the proliferation rate and the susceptibility to senescence of mouse embryonic fibroblasts obtained from HMGA1P6-overexpressing mice to better characterize the HMGA1-pseudogene function. Indeed, our study reports that mouse embryonic fibroblasts (MEFs) derived from HMGA1P6 mice express higher HMGA1 mRNA and protein levels. Moreover, these cells grow faster and senesce later than wild-type sustaining the oncogenic role of ceRNA crosstalk mediated by HMGA1Ps.
- Published
- 2020
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