1. Reduction trend of mcr-1 circulation in Emilia-Romagna Region, Italy
- Author
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Simone Ambretti, Chiara Bracchi, Vittorio Sambri, Carlo Biagetti, Adriana Calderaro, Claudia Venturelli, Giuseppe Diegoli, Ilaria Menozzi, Maria Federica Pedna, Martina Tambassi, Rossella Buttazzi, Carlo Gagliotti, Stefano Pongolini, Mario Sarti, Enrico Ricchizzi, Marianna Meschiari, Maria Luisa Moro, Erika Scaltriti, Massimo Confalonieri, Agostino Barozzi, Marina Morganti, Laura Soliani, Roberta Schiavo, Edoardo Carretto, Luca Bolzoni, Fabio Tumietto, Chiara Casadio, Gagliotti C., Bolzoni L., Carretto E., Sarti M., Ricchizzi E., Ambretti S., Barozzi A., Bracchi C., Confalonieri M., Menozzi I., Morganti M., Pedna M.F., Sambri V., Scaltriti E., Schiavo R., Soliani L., Tambassi M., Venturelli C., Biagetti C., Buttazzi R., Calderaro A., Casadio C., Meschiari M., Tumietto F., Diegoli G., Pongolini S., and Moro M.L.
- Subjects
Microbiology (medical) ,Veterinary medicine ,Antimicrobials for veterinary use ,Antibiotic resistance ,Clonal dissemination ,Klebsiella pneumoniae ,Population ,Drug Resistance ,Horizontal transfer ,Bacterial Protein ,Microbial Sensitivity Tests ,Minimum inhibitory concentration ,Enterobacterales ,One Health approach ,Plasmid ,Bacterial Proteins ,Enterobacteriaceae ,Enterobacterale ,Anti-Bacterial Agent ,Drug Resistance, Bacterial ,medicine ,Humans ,Colistin ,Mcr ,Anti-Bacterial Agents ,Enterobacteriaceae Infections ,Ethanolaminephosphotransferase ,Italy ,Phylogeny ,Retrospective Studies ,education ,education.field_of_study ,biology ,Microbial Sensitivity Test ,Bacterial ,General Medicine ,biology.organism_classification ,Enterobacteriaceae Infection ,Infectious Diseases ,Salmonella enterica ,MCR-1 ,Enterobacter cloacae ,hormones, hormone substitutes, and hormone antagonists ,Human ,medicine.drug - Abstract
This study aims to describe trends of mcr-positive Enterobacterales in humans based on laboratory surveillance with a defined catchment population. The data source is the Micro-RER surveillance system, established in Emilia-Romagna region (Italy), to monitor the trend of mcr resistance. Enterobacterales isolates from human clinical samples with minimum inhibitory concentration (MIC) ≥ 2mg/L for colistin were sent to the study reference laboratory for the detection of mcr genes. Isolates prospectively collected in the period 2018–2020 were considered for the assessment of population rates and trends; further analyses were carried out for the evaluation of clonality and horizontal mcr gene transfer. Previous isolates from local laboratory collection were also described. In the period 2018–2020, 1164 isolates were sent to the reference laboratory, and 51 (4.4%) were confirmed as mcr-positive: 50 mcr-1 (42 Escherichia coli, 6 Klebsiella pneumoniae, 2 Salmonella enterica) and 1 mcr-4 (Enterobacter cloacae). The number of mcr-positive isolates dropped from 24 in the first half of 2018 to 3 in the whole of 2020 (trend p value < 0.001). Genomic analyses showed the predominant role of the horizontal transfer of mcr genes through plasmids or dissemination of transposable elements compared to clonal dissemination of mcr-positive microorganisms. The study results demonstrate a substantial decrease in the circulation of mcr-1 plasmid genes in Emilia-Romagna Region.
- Published
- 2021
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