1. Titanium nitride oxide coating on rough titanium stimulates the proliferation of human primary osteoblasts
- Author
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Durual, Stéphane, Pernet, F., Rieder, Philippe, Mekki, Mustapha, Cattani-Lorente, Maria Angeles, and Wiskott, Anselm
- Subjects
Alloys/chemistry ,Coated Materials, Biocompatible/chemistry ,Plasma Gases ,Dental Etching/methods ,Surface Properties ,Osteocalcin ,Cell Count ,Hydrochloric Acid/chemistry ,Plasma Gases/chemistry ,Cell Proliferation/drug effects ,Dental Materials ,Acid Etching, Dental ,Calcitriol ,Coated Materials, Biocompatible ,Alloys ,Aluminum Oxide ,Cell Adhesion ,Humans ,Calcitriol/pharmacology ,Cell Adhesion/physiology ,Cells, Cultured ,Cell Proliferation ,Titanium ,Osteoblasts ,Osteoprotegerin/analysis ,Reverse Transcriptase Polymerase Chain Reaction ,Photoelectron Spectroscopy ,technology, industry, and agriculture ,Osteoprotegerin ,Alkaline Phosphatase/analysis ,Cell Differentiation ,respiratory system ,equipment and supplies ,Alkaline Phosphatase ,ddc:617.6 ,Osteoblasts/drug effects/physiology ,Aluminum Oxide/chemistry ,Osteocalcin/analysis ,Titanium/chemistry ,Calcium Channel Agonists ,Gene Expression Regulation ,Dental Materials/chemistry ,Dental Etching ,Microscopy, Electron, Scanning ,Cell Differentiation/drug effects/physiology ,Hydrochloric Acid ,Acid Etching, Dental/methods ,Calcium Channel Agonists/pharmacology - Abstract
Titanium is widely used in contemporary endosseous implantology and there is considerable thrust to further promote osseointegration by implant surface modifications. The aim of this study was to evaluate the effect of a titanium-nitride-oxide (TiNOx) coating on commercially pure microroughened titanium by assessing the proliferation and differentiation of human primary osteoblasts.Cell proliferation, gene expression, alkaline phosphatase activity, osteoprotegerin and osteocalcin secretion were analyzed for a time course of 3 weeks, with or without additional stimulation by 1.25(OH)(2) vitamin D(3) 100 nM.A 1.5-fold increase in the proliferation rate of cells grown on TiNOx-coated titanium as compared with uncoated surfaces was observed. SEM views indicated that the cells' normal morphology with their numerous extensions was maintained. The differentiation process on the TiNOx surface was only affected to a minor degree and translated into a slight delay in osteoblast maturation when compared to uncoated titanium.Pending confirmation of these results in vivo, TiNOx coatings could potentially accelerate and enhance osseointegration.
- Published
- 2010