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Habaek koreanus gen. n., sp. n., a remarkable new Perlodinae is described from adults of both sexes, collected by Malaise traps in South Korea. On the basis of male characters, it is assigned to the tribe Perlodini, but its tribal classification needs to be clarified by characters of the larva and matured eggs. Affinities of the new genus are discussed, and a key is presented for the males of the Perlodini genera.

Five Filchneria Klapálek, 1908 (Plecoptera, Perlodidae) species known since the last century (F. balcarica Balinsky, F. mesasiatica Zhiltzova, F. irani (Aubert), F. kuenluensis (Šámal), and F. olgae (McLachlan)) were redescribed on types stored in museums in the Netherlands, Switzerland, Russia and newly acquired material. The redescription were in accordance with the requirements of modern taxonomy, illustrated with color photographs of the habitus, head, pronotal patterns, eversible male paraproct lobes, male paraproctal sclerite, aedeagus (as possible), details of chaetotaxy, female subgenital plate, and egg using digital cameras and SEM. The diagnostic features of each species are revealed. The main differences were established in the male paraproctal sclerite shape, in the posterior margin of tergum 10, the female subgenital plate, and the egg structures. After reexamining the syntype series of Filchneria olgae, a lectotype is designated for this species. Filchneria kuenluensis is proposed to transfer to Perlodinella Klapálek, 1912 with the valid name Perlodinella kuenluensis (Šámal, 1935) comb. nov. Filchneria shobhaae is considered as a junior synonym of Zhiltzovaia amabilis (Jewett, 1958) syn. nov.

Perlodinella kuenluensis (Šámal, 1935) comb. nov. Figs. 23–34 Šámal, 1935: 221, figs. 1a, b, Perlodes (Megarcys) kuen-luensis. Dry specimens one male and one female with red labels ‘Typus’ from Coll. Zôlogisch Museum Amsterdam deposited in Naturalis, Leiden, the Netherlands. Claassen, 1940: 113 Perlodes kuen-luensis; Brinck, 1950: 137, figs. 3A, 3C, Perlodes (Perlodinella) kuen-luensis; Ricker, 1952: 140, Skobeleva (Megarcys) kuen-luensis incorrect original spelling; Brinck, 1954: 137, Skobeleva kuenluensis; Illies, 1966: 389, Skobeleva kuenluensis; Zwick, 1973: 229, Filchneria kuenluensis; Li & Murányi, 2015: 51, Filchneria kuenluensis. Diagnosis. Filchneria kuenluensis is a fairly dark species, having an oval narrow pale spot in the interocellar area. The male of F. kuenluensis is distinguished by the shape of the posterior margin of tergum 10 which is strongly elevated and drawn out, appearing as a conical hood, pointed at the apex, and covered with sensilla basiconica dorsally. The paraproct is finger like and paraproctal sclerite bears a hook near to the rounded top. The female is distinguished by a strongly sclerotized triangular bilobed subgenital plate that is expanded posteriorly. The posterior margin of the subgenital plate has a triangular notch in the middle dividing two triangular lobes. The posterolateral edge of each lobe is rounded and slightly drawn downward laterally. Supplementary description. The original description of F. kuenluensis was carried out on male and female syntypes as Perlodes (Megarcys) kuen-luensis, including sizes and color pattern (Šámal 1935). Later, Brink (1950) pointed out briefly the male paraproct structures which were omitted in the original description. A supplementary description of the type material, taking into account its age and state of preservation is given below. Head brown with a dark brown M-line; median interocellar area dark brown with an oval narrow pale spot (Figs. 23, 30). Occipital area is brown. Pronotum dark brown with even darker narrow strip along anterior and posterior margins, rugosities are dark brown (Figs. 23, 30). Lateral arms of meso-furcasternum meet posterior corners of furcal pits (Fig. 28). Male. Syntype (Fig. 27). Abdominal terga 7–9 swollen posterolaterally, posteromedial margins covered with sensilla basiconica and very long brown hairs (Fig. 26). Tergum 10 entire, in lateral view with strongly elevated and drawn out posterior margin, appearing as conical hood, pointed at the apex, and covered with sensilla basiconica dorsally (Figs. 24–26). Paraproct finger-shaped, swollen, narrowed distally, and rounded at the top (Figs. 24, 25). The paraproctal sclerite wide and heavily sclerotized basally, in apical half paraproctal sclerite slender, bears hook directed outward (Fig. 25). Naturally everted aedeagus looks like a membranous tube with a pair of lateral lobes at the base, ventrally, aedeagal apex narrowed, funnel-shaped with cuticular swellings (Fig. 24). Female. Syntype (Fig. 34). Abdominal sterna are pale-brown (Figs. 31, 32). Subgenital plate strongly sclerotized, triangular, bilobed expanding posteriorly, and occupying about half of sternum 8 width, and one third of sternum 9 lengths (Figs. 31, 32). The posterior margin with a triangular notch in the middle divides plate into two triangular lobes. The posterolateral edge of each lobe is rounded and slightly drawn downward laterally (Fig. 32). Sternum 9 bears brown patch on each side. Egg. Trilateral, broadly oval, longitudinal ridges delimit the three sides. Apparently there is a collar (Fig. 33). Other details and the chorion structure are indistinctive. Material examined. Coll. Zôlogisch Museum Amsterdam. Typus. Male. ZMA. INS. 5101771. P. kuen-luensis. Typus. Female. ZMA. INS. 5101021. Perlodes kuen-luensis. Filchneria kuenluensis (Šámal). Between Sanju Basar & Suget-Karaul, 1880–3600 m, 19–31. V.1930. Nederlandsche Karakorum Expeditie, J. A. Sillem leg. Det. Dr. Šámal. Det. P. Zwick 1979 (Naturalis). Distribution. The species is only known from the types collected in Tibet Plateau, Kunlun Mountains, China, Xinjiang, between Sanju Bazar and Suget Karaul (the modern 'Saitula' town). Remarks. The shape of the posterior margin of tergum 10 of F.kuenluensis male resembles that of F.mesasiatica; however, the paraproctal sclerite of F. mesasiatica lacks an apical hook. The male of Zhiltzovaia amabilis (Jewett, 1958) has the paraproct sclerite with a narrow, truncated knob dorsally and larger rounded knob ventrally, while in F. kuenluensis the ventral knob at the base of the cerci was not found (Fig. 24). The male of F. kuenluensis in shape of tergum 10 and the paraproct structure is similar to that of Perlodinella kozlovi Klapálek, 1912 (Fig. 29) and to Perlodinella apicalis Kimmins, 1947 consistent with Brink (1950), who assigned F. kuenluensis on these features to typical Perlodinella (Klapálek 1912). Since, according to recent publication (Huo et al. 2022) a clawlike process in the apical part of paraproctal sclerite is one important diagnostic feature of the Perlodinella, we proposed to transfer Filchneria kuenluensis to Perlodinella with the valid name Perlodinella kuenluensis (Šámal, 1935) comb. nov., Published as part of Teslenko, Valentina A. & Palatov, Dmitry M., 2023, Redescription of a few Filchneria Klapálek, 1908 (Plecoptera, Perlodidae) species on the type and fresh material, pp. 287-312 in Zootaxa 5277 (2) on page 295, DOI: 10.11646/zootaxa.5277.2.3, http://zenodo.org/record/7889837, {"references":["Samal, J. (1935) Plecoptera. In: Visser, P. & Visser-Hooft, J. (Eds.), Wissenschaftliche Ergebnisse der Niederlandischen Expeditionen in den Karakorum und die angrenzenden Gebiete in den Jahren 1922, 1925 u 1929 / 30. Vol. 1. Brockhaus, Leipzig, pp. 221 - 225.","Claassen, P. W. (1940) A Catalogue of the Plecoptera of the World. Agricultural Experiment Station Memoir 232. Cornell University Press, Ithaca, New York, 235 pp.","Brinck, P. (1950) The 3 rd Danish Expedition to Central Asia. Zoological Results, 1, Plecoptera (Insecta) from Afghanistan. Videnskabelige meddelelser Fra Dansk Naturhistoriske forening i Kjobenhavn, 112, 133 - 138.","Ricker, W. E. (1952) Systematic studies on Plecoptera. Indiana University Publications, Science Series 18. Indiana University, Bloomington, Indiana, 200 pp.","Brinck, P. (1954) On the classification of the plecopteran subfamily Perlodinae. Opuscula entomologica, 19, 190 - 201.","Illies, J. (1966) Katalog der rezenten Plecoptera. Das Tierreich, Berlin, 82, 1 - 632.","Zwick, P. (1973) Insecta: Plecoptera. Phylogenetisches System und Katalog. Das Tierreich 94. Walter de Gruyter, Berlin, xxxii + 465 pp.","Li, W. H. & Muranyi, D. (2015) A remarkable new genus of Perlodinae (Plecoptera: Perlodidae) from China, with remarks on the Asian distribution of Perlodinae and questions about its tribal concept. Zoologischer Anzeiger, 259, 41 - 53. https: // doi. org / 10.1016 / j. jcz. 2015.10.003","Jewett, S. (1958) Entomological survey of the Himalaya. Part 23. Stoneflies (Plecoptera) from the north-west (Punjab) Himalaya. Proceedings of the National Academy of Science of India, Allahabad, 28 (4), 320 - 329.","Klapalek, F. (1912) Plecopteres I. Fam. Perlodidae. In: Collections Zoologiques du Baron Edm. Vol. 4. de Selys-Longchamps, Bruxelles, pp. 1 - 66.","Kimmins, D. E. (1947) [1946] New species of Himalayan Plecoptera. Annals and Magazine of Natural history, Series 11, 13 (107), 721 - 740. https: // doi. org / 10.1080 / 00222934608654596","Huo, Q. B., Du, Y. - Z., Zwick, P. & Muranyi, D. (2022) Notes on Perlodinella Klapalek, 1912 (Plecoptera: Perlodidae) with a new species and a new synonym. Zootaxa, 5162 (4), 378 - 396. https: // doi. org / 10.11646 / zootaxa. 5162.4.3"]}

Filchneria irani (Aubert, 1964) Figs. 16–22 Aubert, 1964: 79, figs 32–34 (Perlodes (Skobeleva) irani). Holotype and paratype are stored in ethanol and deposited in MZL; lllies, 1966: 389 (Skobeleva irani); Zwick, 1973: 229 (Filchneria irani); Sartori et al., 1990: 170 (Filchneria irani); Zwick, 1997: 495, fig. 7d (Filchneria irani); Mohammadian, 2008: 113–115, (Perlodes(Skobeleva) irani). Diagnosis. The male of F. irani is distinguished by the shape of the posterior margin of tergum 10, which is slightly elevated and its top smoothly rounded; the paraproctal sclerite is triangular and heavily sclerotized basally and along the outer edge, sclerotization narrowed distally, and indistinct at the apex. The female has a wide and short subgenital plate, its posterior margin slightly serrated; the lobes are poorly expressed, each lateral edge bears a small rounded protrusion directed to the side. Complementary description. Adult habitus. Color pattern of male and female on the head and pronotum is similar(Figs. 16,17, 20).Head is brown with pale spots.Most parts of clypeus are covered with a semicircular brownish spot; anterior edge, paired small spots on lateral clypeal margins near M-line bases, and small butterfly-shaped spot in front of anterior ocellus are pale. M-line brown, interocellar area brown, with a rather narrow longitudinal pale stripe slightly widened posteriorly. Occiput with transverse pale band extends along the epicranial stem and compound eyes (Figs. 16, 17, 20); a narrow and short black band surrounds each compound eye posterolaterally. Pronotum brown with pale median band widened posteriorly, anterior corners bear small pale spots, the pronotal rugosities are dark brown, sometimes indistinct (Figs. 17, 20). Male. Holotype. Brachypterous, wings not exceeding tergum 2 (Fig. 16). Coloration of abdominal terga corresponds to original description: large, yellowish quadrangular spot on sides of brown median line of terga 1–7 (Fig. 16). Dark medial line is obscured on terga 8–9, tergum 10 entirely yellowish. Tergum 7 bears two patches of sensilla basiconica on sides of dark medial line posteriorly; tergum 8 densely covered by sensilla basiconica posteromedially; the sensilla basiconica are also present on tergum 9, but they are solitary and scattered closer to the posterior tergal margin (Fig. 18). Posterior margin of tergum 10 slightly elevated, top smoothly rounded, dorsal surfaces covered with sensilla basiconica in the middle (Figs. 18, 19). Paraproct is triangular, wide at the base and narrowed to the apex. The paraproctal sclerite triangular, heavily sclerotized basally and along outer edge, narrowed to the apex, and indistinct; sclerotization along outer sclerite edge serrated, (Fig. 19); inner edge of paraproct is pale and membranous distally. Female. Paratype. Macropterous. Sternum 8 is dark brown in the anteromedian half, bears a pair of oblique, dark brown lateral sclerites surrounding a subgenital plate anterolaterally (Fig. 21). Subgenital plate wide and short, covering about one third of sternum 9; the posterior margin with a slight notch in the middle; the lobes are poorly expressed, enclosed with tiny, brown setae; each lobe at the lateral edge with a small, rounded, lateral protrusion, posterior half of subgenital plate brownish (Fig. 21). Sternum 9 is pale medially, with pair of rounded dark brown spots laterally extending to curve brown bands directed upward under the subgenital plate (Fig. 21). Sternum 10 pale with two small brown spots laterally. Paraprocts are triangular in shape, rounded at the apex. Egg. According to P. Zwick (1997) the egg measures 430×300 μm. Egg has almost parallel lateral margins before anterior pole, the anterior pole is arcuate, posterior one truncated, pedicel very short, the anchor plate flat, pan-shaped (Fig. 22). The chorion is not distinctive. Material examined. Holotype male, paratype female: Iran, prov. Ostan, Chaîne de l'Elbourz, Polur, 2800 m, 10.V. 1956. F. Schmid leg. (MZL). Distribution. Filchneria irani was collected by F. Schmid in northern Iran (Aubert 1964), in Polur village which is situated on the Haraz River in Mazandaran Province. The Haraz River originates from the east slope of Mount Damavand (5610 m asl), Central Elburz (Alborz) Mountain Range and flows in a relatively narrow valley into the Caspian Sea. Schmid has collected material until altitude 2800 m asl. This is the only record of F. irani, an apparently rare species, which is considered an endemic of the Elburz Mountains (Mohammadian 2008)., Published as part of Teslenko, Valentina A. & Palatov, Dmitry M., 2023, Redescription of a few Filchneria Klapálek, 1908 (Plecoptera, Perlodidae) species on the type and fresh material, pp. 287-312 in Zootaxa 5277 (2) on pages 292-295, DOI: 10.11646/zootaxa.5277.2.3, http://zenodo.org/record/7889837, {"references":["Aubert, J. (1964) Plecopteres du nord de l'Iran. Mitteilungen der Schweizerischen Entomologischen Gesellschaft, 37 (1), 69 - 80.","Zwick, P. (1973) Insecta: Plecoptera. Phylogenetisches System und Katalog. Das Tierreich 94. Walter de Gruyter, Berlin, xxxii + 465 pp.","Sartori, M., Membiela, P., Ravizza, C., Sanchez-Ortega, A. & Zwick, P. (1990) Liste des types deposes au Musee de zoologie, Lausanne. 1. Plecoptera (Insecta). Bulletin de la Societe Vaudoise des Sciences Naturelles, 80 (2), 164 - 190. http: // doi. org / 10.5169 / seals- 279557","Zwick, P. (1997) Rauserella, a new genus of Plecoptera (Perlodidae), with notes on related genera. In: Landolt, P. & Sartori, M. (Eds.), Ephemeroptera & Plecoptera. Biology-Ecology-Systematics. MTL-Mauron + Tinguely & Lachat, S. A. Fribourg, pp. 489 - 496.","Mohammadian, H. (2008) Aquatic Insects of Iran. Plecoptera. Vol. 2. Shabpareh Publications, Tehran, 184 pp. [in Farsi]"]}

Filchneria olgae (McLachlan, 1875) Figs. 49–83 McLachlan, 1875: 49, Table IV, figs. 10 a, b, Dictyopteryx Olgae. Lectotype, male and paralectotypes (1 male 4 females) deposited in ZMMU, Moscow, Russia; Klapálek, 1912: 23, Figs. 19 A, B (Skobeleva Olgae); Ricker, 1952: 140, figs. 86, 88, 89, (Skobeleva olgae); Illies, 1966: 390, Skobeleva olgae; Raušer, 1968: 362, (Skobeleva olgae); Zhiltzova, 1971: 1035, (Dictyopteryx olgae); Zwick, 1973: 228, 230, (Filchneria olgae); Zwick, 1997: 494, figs. 6 (b‒d), Teslenko & Zhiltzova, 2009: 24, 102–104, (Filchneria olgae). Diagnosis. Males of F. olgae have an obtuse angled (angle about 120°) posterior margin of tergum 10. The paraproctal sclerite is short, wide, and strongly sclerotized, resembling a rectangle with a Ⅽ-shaped notch along the inner margin; the apices of the notch are sharp and short. The aedeagus bears unpaired anterodorsal and posteroventral lobes, and two pairs of lateral lobes with one pair of lateral lobes being fingerlike. Females of F. olgae are distinguished by a short, wide subgenital plate with a straight or slightly wavy posterior margin, the lobes being weakly expressed, and the lateral edges are slightly truncated. Eggs of F. olgae bear clear longitudinal and transverse ridges, a stalked collar, and a rim that is slightly triangular with a wavy outer edge, and a smooth longitudinal carina present. The chorion is rough and evenly covered with flat rounded tubercles that have a distinct groove at the base. Micropyle orifices lack lip-like extensions. Complementary description. Adult habitus. Filchneria olgae was firstly described based on male and female by MacLachlan (1875) on the materials by A.P. Fedchenko outstanding explorer of the Central Asia. Type seria of F. olgae specimens is shown below (Fig. 49). The original description was carried out on base of the coloration and shape of the female subgenital plate and, in general, is consistent with the studied specimens (McLachlan 1975). Complementary description is given below based on the type material and relatively fresh specimens. Both sexes may have shortened wings, males may also be brachypterous, females ‒ macropterous; wings are transparent with brown veins; the venation configuration is typical to Filchneria (Figs. 52, 55, 65, 66). The general body color brown, males are dark brown (Figs. 65, 66). Head brown, M-line brown (Figs. 51, 56, 67, 68). The interocellar area carries a small yellow spot slightly widened posteriorly (Figs. 51, 56, 67, 68). A yellow Ushaped band extends across the occiput, forming triangular medial projections along the epicranial stem; a brown posterolateral spot behind each compound eye (Figs. 51, 56, 67, 68). Antennae are brown, palpi pale. The pronotum brown with relatively thin yellow median band slightly widened posteriorly, the pronotal rugosities dark brown forming X-shaped pattern (Figs. 65–68). Legs are brown, with wide brown band on femur and dark brown stripe on tibia basally (Fig. 66). Cerci light brown, each cercal segment darker distally (Fig. 65). Male. Body length 13.3‒16.8 mm (n=5). Wings short, not reaching the posterior margin of tergum 3 (Figs. 52, 66). Terga 4‒9 humped laterally. Abdominal terga 1–6 brown, sometimes longitudinal row of 8–12 very small pale spots visible; the middle pair of spots is more pronounced than the others (Fig. 66). Terga 7–8 with butterfly-shaped pale sport; in tergum 8 a pale spot expanded posteriorly and divided by a median, triangular, and pale spot, widened anteriorly; two submedial swellings densely covered by sensilla basiconica and brownish hairs close to the posterior margin (Figs. 52, 66, 69). Tergum 9 is half hidden under tergum 8, sclerotized laterally; posteromedial half membranous with two swellings, covered with sensilla basiconica and brownish hairs posterolaterally (Fig. 69). Tergum 10 pale in caudal view up curved (Figs. 52–54, 66, 69–71); in dorsal view the posterior margin of tergum 10 with thin brown line, obtuse angled (angle about 120°), bears posteriomedial patch of short and sparse sensilla basiconica distributed to one-thirds of the tergum length in the middle (Figs. 54, 74). The paraproctal sclerite is short, wide, and strongly sclerotized, resembling a rectangle with a Ⅽ-shaped notch along the inner margin; the apices of the notch are sharp and short (Figs. 53, 66, 69–71). In repose paraproctal sclerite surrounds a triangular membranous lobe rounded at the top, coated by fine sensory scales and small thin spinules dorsally and dorsomedially (Figs. 69, 71). Naturally everted aedeagus membranous, short and wide bears unpaired anterodorsal and posteroventral lobes, and two pairs of lateral lobes (Figs. 71, 73). The anterodorsal lobe spherical dorsally; a cuticular triangular swelling adjoins the anterodorsal lobe ventrally with anterolateral fingerlike cuticular lobes directed downward, theirs apices are narrowed (Figs. 71, 72). At the bases of anterolateral lobes there are additional small lateral swellings ventrally which adjacent to unpaired, large, and transverse posteroventral lobe, bearing large posterolateral swellings resemble auricles; in ventral view the lobes are fan-shaped (Fig. 73). Female. Body length 17.8‒21.5 mm (n=5). Sterna 1‒7 yellow with a thin brownish band anteriorly and a pair of small brownish spots laterally (Figs. 75, 76). Sternum 8 pale with a pair of rounded brown spots laterally and a pair of oblique dark brown lateral sclerites surrounding the brownish subgenital plate anterolaterally (Figs. 57, 58, 75, 76). Subgenital plate is short and wide, extending about three-quarters of the sternum 8 widths (along posterior margin) and about a quarter of the sternum 9 lengths, with an almost straight or slightly wavy posterior margin; the lobes are weakly expressed; the lateral edges are slightly truncated (Figs. 58, 75,76). Subgenital plate covered with short brown setae (Figs. 75, 76). Sternum 9 medially pale with a pair of brown rounded spots laterally, turning into curved brown stripes directed upwards under the subgenital plate (Figs. 58, 75, 76). Sternum 10 is usual, pale. Paraprocts are dark brown at the base of cerci; tips are pale. Egg. Medium size, trilateral, with mean dimensions of 416×274 μm (n=11). The transverse and longitudinal ridges are clearly visible, the longitudinal ridges are rather thick and bulged (Figs. 59, 60, 64, 77, 78). The collar is stalked, rim slightly triangular with a wavy outer edge, dorsally (Figs. 61–63, 78, 79, 81). The sides of the collar bear smooth longitudinal carinae (Fig. 81). The anchor plate mushroom-shaped or pan-shaped evenly covered with large globular bodies (Figs. 77, 79, 80). A transverse row of 4‒6 micropyles subequatorial, slightly closely to transverse ridge (Figs. 59, 60, 77, 78); micropyles clearly visible, their orifices without lips, some set close to micropyles mounds (Fig. 82). The chorion surface is rough with numerous flat rounded tubercles, located relatively evenly over the entire surface (Figs. 59, 60, 77, 78, 82). The base of the tubercles has a distinct groove (Fig. 83). Material examined: Lectotype male (present designation), paralectotypes 1 male, 4 females, Uzbekistan, Samarkand city, vicinity, Zeravshan River Basin (authors note), 14 March, 1869, coll. Fedchenko A.P., det. McLachlan. Lectotype: № ZMMU Ple-0001, Zool. Mus. Mosq. Univ. (Mosquae, ROSSIA) (Fig. 50). Uzbekistan, Western Tian-Shan, Talas Alataw, 3 males 3 females, Chirchiq River in the area of construction of the Charvan power station (Syr-Darya River Basin), 28.03.1968, (ZIN). Tajikistan, Pamir-Alay mountain system, 2 males 2 females, Ramit Nature Reserve, Sardan-Miyona River, 17.03.1987, coll. L. Zhiltzova (ZIN). Distribution. Filchneria olgae has an early spring emergence in March. It inhabits mountain watercourses of interfluves of the large Central Asian Amu-Darya and Syr-Darya Rivers at elevations of 700 m and above, the upper limit of the habitat has not been established. In Uzbekistan the species was found in the vicinity of Samarkand city, located in the middle reaches of the Zeravshan River (Amu-Darya R. Basin) and in the Chirchiq River (Syr-Darya R. Basin) in West Tian Shan, Talas Alataw. Also F. olgae was found in Tajikistan (State Natural Reserve "Ramit"), in the Sardai-Miyona River which flows down from the southern slopes of the Gissar Ridge of the Pamir-Alai Mountains, stretching over 200 km from the east to the west direction across Tajikistan and Uzbekistan., Published as part of Teslenko, Valentina A. & Palatov, Dmitry M., 2023, Redescription of a few Filchneria Klapálek, 1908 (Plecoptera, Perlodidae) species on the type and fresh material, pp. 287-312 in Zootaxa 5277 (2) on pages 302-307, DOI: 10.11646/zootaxa.5277.2.3, http://zenodo.org/record/7889837, {"references":["McLachlan, R. (1875) Neuropterans (Neuroptera). Perlidae. In: Fedchenko A. P. \" Travel to Turkestan. Zoogeographical investigations \". 5, 5. Publishing house of the Society of amateurs of the natural history, anthropology, ethnography in Moscow, 19 (1), 49 - 155.","Klapalek, F. (1912) Plecopteres I. Fam. Perlodidae. In: Collections Zoologiques du Baron Edm. Vol. 4. de Selys-Longchamps, Bruxelles, pp. 1 - 66.","Ricker, W. E. (1952) Systematic studies on Plecoptera. Indiana University Publications, Science Series 18. Indiana University, Bloomington, Indiana, 200 pp.","Illies, J. (1966) Katalog der rezenten Plecoptera. Das Tierreich, Berlin, 82, 1 - 632.","Rauser, J. (1968) Plecoptera. Ergebnisse der zoologischen Forschungen von Dr. Z. Kaszab in der Mongolei. Entomologische Abhandlungen, Staatliches Museum fur Tierkunde in Dresden, 34, 329 - 398.","Zhiltzova, L. A. (1971) The genus Filchneria Klap. and its position in the family Perlodidae (Plecoptera). Zoological Journal, 50, 1034 - 1040. [in Russian]","Zwick, P. (1973) Insecta: Plecoptera. Phylogenetisches System und Katalog. Das Tierreich 94. Walter de Gruyter, Berlin, xxxii + 465 pp.","Zwick, P. (1997) Rauserella, a new genus of Plecoptera (Perlodidae), with notes on related genera. In: Landolt, P. & Sartori, M. (Eds.), Ephemeroptera & Plecoptera. Biology-Ecology-Systematics. MTL-Mauron + Tinguely & Lachat, S. A. Fribourg, pp. 489 - 496.","Teslenko, V. A. & Zhiltzova, L. A. (2009) Keys to the stoneflies (Insecta, Plecoptera) of Russia and adjacent countries. Imagines and larvae. Dalnauka, Vladivostok, 382 pp. [in Russian]"]}

Filchneria mesasiatica Zhiltzova, 1971 Figs. 35–48 Zhiltzova, 1971: 1036–1038, fig. 2. Holotype and allotype deposited in ZIN, St. Petersburg, Russia; Zwick, 1973: 229; Zhiltzova, 1995: 9; Zwick, 1997: 494–495, fig. 7b; Teslenko & Zhiltzova, 2009: 23–24, figs. 93–97. Diagnosis. Filchneria mesasiatica is a relatively large species, differing in the strongly raised triangular posterior margin of tergum 10 whose tip is acute, angled (about 80°), and rounded. The paraproctal sclerite is triangular with a wide base that is narrowed distally, and the tip is slightly sclerotized, indistinct, but without a tooth, and the inner edge is slightly concave. The paraproctal membranous lobe is narrow. The ELP enlarges into a membranous lobe with a small papilla atop. The female subgenital plate is species specific, resembling a wrench head. Its form is bilobed, with U-shaped rounded and deep notch, the lobes prolonged, triangular with wide base, apices pointed, directed inward, outer lateral edges obliquely cut. Eggs are relatively large sized, oval, and trilateral; its longitudinal and transverse ridges are slightly bulged and indistinct. The collar is formed by weakly noticeable extensions of the three longitudinal ridges, the edge being irregularly incised. The chorion is rough with numerous raised tubercles, densely deposited over the entire surface. Complementary description. Male. Holotype. Body length is 19.0 mm; brachypterous, wings slightly exceeding tergum 3 (Fig. 36). Abdominal terga 6–8 humped laterally; brown, color pattern indistinct, paired narrow pale patches are obvious on posterolateral margins of each tergum, terga 6‒7 bear medial triangular pale spots narrowed to posterior margins (Fig. 36). Tergum 8 brown, divided by a triangular median pale spot anteriorly; two submedial swellings densely covered by sensilla basiconica and brownish hairs posterolaterally. Tergum 9 has an anteromedial triangular membranous area hidden under tergum 8; sclerotized laterally; posteromedial half slightly sclerotized with two swellings covered by sparse thick sensilla basiconica and brownish hairs posterolaterally (Figs. 36, 37). In the lateral and caudal views posterior margin of tergum 10 is strongly raised, acute angled, tip rounded, directed upward; in dorsal view, the posteromedial margin triangular, covered with a patch of sensilla basiconica in V-shaped direction from tip to lateral edges, occupying one-thirds of the tergum length in the middle (Figs. 36–38, 40). At rest, the paraproct is triangular with convex dorsomedial edge (Figs. 36, 37). The paraproct sclerite is triangular, with wide and sclerotized base, narrowed distally, top slightly sclerotized, indistinct, but without tooth, inner edge concave, membranous, covered with tiny, light brown sensory spines and sparse fine sensory hairs (Figs. 36, 37). Artificially everted eversible paraproct lobe (EPL) enlarges into a membranous lobe with a small papilla atop, and covered by sparse fine sensory hairs (Figs. 38, 39). Female. Allotype. Body length 22.5 mm, macropterous, forewing length 22.0 mm, wingspan 31.0 mm. Sternum 8 pale with a few pairs of brown spots laterally and medially, anterior half brownish, a pair of oblique dark brown lateral sclerites surrounds subgenital plate anterolaterally (Fig. 42). Subgenital plate large, bilobed resembles a wrench, wide at the base, occupies more than half of the sternum 8 widths and of the sternum 9 lengths (Fig. 42). Notch U-shaped, rounded, deep; depth exceeds half of the lobes length; lobes triangular, wide at the bases, prolonged, apices pointed, dark brown, directed inward, outer lateral edges obliquely cut (Fig. 42). Subgenital plate mostly pale covered with sparse, tiny, dark setae, a paired small brown lateral spots at the lobe bases indistinct (Fig. 42). Sternum 9 pale medially with pair of brown rounded spots laterally extending to curve brown bands directed upward under the subgenital plate lobes (Fig. 42). Abdominal sternum 10 is pale. Egg. Allotype. Large, oval, and trilateral, with mean dimensions of 786×504 μm (n=4). The longitudinal ridges are slightly bulged; transverse bridges indistinct (Figs. 43, 44). The collar short, formed by weak, barely noticeable extensions of the three longitudinal ridges, the edges irregularly incised (Fig. 46).The anchor plate mushroom-shaped covered with sparse, large globular bodies, especially at edge (Figs. 44, 45). A transverse row of 4‒6 micropyles subequatorial, their orifices lipped, some set close to micropylar mounds (Figs. 43, 48); the height of the micropyles is equal to or slightly exceeds the height of the chorionic tubercles. The chorion surface is rough with numerous raised tubercles evenly and densely located over the entire surface of the egg (Figs. 43, 46, 48). Material examined. Holotype male. Allotype female. Kyrgyzstan, Tian Shan, Alamedin River at the Strelnikova farm, to the south of Frunze town [now Arshan vill., near Bishkek town, authors comments], 11.05.1967, coll. L. Zhiltzova (ZIN). Additional material: 2 males, 2 females, Kyrgyzstan, Alay Ridge, Kirgiz-ata River, 22.06.1970, coll. L. Zhiltzova (ZIN); 1 female, Kyrgyzstan, Chui Region, Ala-Archa River at the confluence of the Karabulak River, altitude 2.061 m above sea level, a rocky canyon 5 m wide, 0.5 m deep, current speed 0.3–1.0 m/s, water temperature 12 °C, sand near the coast, N 42°36.200’, E 74°28.819’, 28.05.2016, coll. DMP (VLA); Tajikistan. Pamir-Alai mountain system, Gorno-Badakhshan Autonomous Region: 1 male, Shugnan District, Bogevdara R., 1 km above the mouth, near the Bogev vill., 6.06.2012, N 37°33.5184’, E 71°44.1642’, coll. DMP (VLA); 1 male, 1 female, Shugnan District, Gunt River, left tributary near the Kolkhozobol vill., altitude 2.450 m above sea level, 3.06.2012, N 37°33.5184’, E 71°44.1642’, coll. DMP (VLA). Remarks. Paraproctal sclerite of F. mesasiatica is similar in shape to that of F. irani and to F. wusuensis Chen, 2019 described recently from East Tien Shan, China (Chen 2019, Teslenko & Palatov 2021). The female subgenital plates of F. mesasiatica and F. irani are very different, while F. mesasiatica and F. wusuensis females have a similar shape of the notch on the bilobed subgenital plate, with almost straight inner angles. The egg structures of F. wusuensis are poorly visible due to use of a low magnification and lack of scanning electronic imaging, making it impossible to compare with that of F. mesasiatica. Further research will show whether F. wusuensis is a valid species or a junior synonym of F. mesasiatica. Distribution. North and Central Tian Shan and Pamir-Alai mountain system (Kyrgyzstan, Tajikistan). Emergence period is in May-June., Published as part of Teslenko, Valentina A. & Palatov, Dmitry M., 2023, Redescription of a few Filchneria Klapálek, 1908 (Plecoptera, Perlodidae) species on the type and fresh material, pp. 287-312 in Zootaxa 5277 (2) on pages 298-300, DOI: 10.11646/zootaxa.5277.2.3, http://zenodo.org/record/7889837, {"references":["Zhiltzova, L. A. (1971) The genus Filchneria Klap. and its position in the family Perlodidae (Plecoptera). Zoological Journal, 50, 1034 - 1040. [in Russian]","Zwick, P. (1973) Insecta: Plecoptera. Phylogenetisches System und Katalog. Das Tierreich 94. Walter de Gruyter, Berlin, xxxii + 465 pp.","Zhiltzova, L. A. (1995) Katalog tipovykh ekzemplyarov kollektsii Zoologicheskogo Instituta RAN. Vesnyanki (Plecoptera) [Catalogue of type specimens in the collection of the Zoological Institute, Russian Academy of Sciences. Stoneflies (Plecoptera). ZIN RAN Press, St. Petersburg, 38 pp. [in Russian]","Zwick, P. (1997) Rauserella, a new genus of Plecoptera (Perlodidae), with notes on related genera. In: Landolt, P. & Sartori, M. (Eds.), Ephemeroptera & Plecoptera. Biology-Ecology-Systematics. MTL-Mauron + Tinguely & Lachat, S. A. Fribourg, pp. 489 - 496.","Teslenko, V. A. & Zhiltzova, L. A. (2009) Keys to the stoneflies (Insecta, Plecoptera) of Russia and adjacent countries. Imagines and larvae. Dalnauka, Vladivostok, 382 pp. [in Russian]","Chen, Z. T. (2019) A new brachypterous Filchneria and two new generic records for China, Arcynopteryx and Pictetiella (Plecoptera: Perlodidae). Zootaxa, 4679 (3), 511 - 526. https: // doi. org / 10.11646 / zootaxa. 4679.3.5","Teslenko, V. A. & Palatov, D. M. (2021) A poorly known species and new records of Plecoptera from the Eastern Tien Shan, Xinjiang Uygur Autonomous Region, China. Zootaxa, 4950 (1), 123 - 136. https: // doi. org / 10.11646 / zootaxa. 4950.1.6"]}

Zhiltzovaia amabilis (Jewett, 1958) = Filchneria shobhaae (Singh & Ghosh, 1969) syn. nov. Fig. 84 Singh & Ghosh, 1969: 134–135, fig. 1 (Perlodes shobhaae). Holotype female and paratypes 10 females deposited in the National Zoological collection of Zoological Survey of India, Calcutta; Zwick, 1973:230 (Filchneria shobhaae); Chandra et al. 2019: 229 (Filchneria shobhaae). Remarks. The redescription of Zhiltzovaia amabilis (Jewett, 1958), based on type and new material collected from the streams of Pamir (Tajikistan) and the northwestern Himalaya, including northern India (Teslenko & Palatov 2020) agrees well with the original relatively brief description of the female of Filchneria shobhaae by Singh & Ghosh (1969). Taking into account the remarkable shape of the female subgenital plate (Fig. 84), the color pattern of the head, pronotum, legs, cerci, and the proximity of the type localities of F. shobhaae with recent records of Z. amabilis in the basin of the same Alaknanda River, in the state Unttarakhaand, India, and an identical time of emerging, we believe the species are considered conspecific. It also follows that F. shobhaae belongs to the genus Zhiltzovaia Özdikmen, 2008. We propose to transfer Filchneria shobhaae to genus Zhiltzovaia and place F. shobhaae as a junior synonym of Zhiltzovaia amabilis ( Jewett, 1958) syn. nov., Published as part of Teslenko, Valentina A. & Palatov, Dmitry M., 2023, Redescription of a few Filchneria Klapálek, 1908 (Plecoptera, Perlodidae) species on the type and fresh material, pp. 287-312 in Zootaxa 5277 (2) on page 309, DOI: 10.11646/zootaxa.5277.2.3, http://zenodo.org/record/7889837, {"references":["Jewett, S. (1958) Entomological survey of the Himalaya. Part 23. Stoneflies (Plecoptera) from the north-west (Punjab) Himalaya. Proceedings of the National Academy of Science of India, Allahabad, 28 (4), 320 - 329.","Singh, R. K. & Ghosh, S. K. (1969) On a new species of the genus Perlodes (Plecoptera: Perlodidae) from India. Zoologischer Anzeiger, 182 (1 / 2), 134 - 136.","Zwick, P. (1973) Insecta: Plecoptera. Phylogenetisches System und Katalog. Das Tierreich 94. Walter de Gruyter, Berlin, xxxii + 465 pp.","Chandra, K., Gupta, D. & Ishtiaq, A. (2019) A Catalogue of Indian Stoneflies (Insecta: Plecoptera). Zootaxa, 4646 (2), 201 - 235. https: // doi. org / 10.11646 / zootaxa. 4646.2.1","Teslenko, V. A. & Palatov, D. M. (2020) Redescription of the remarkable Zhiltzovaia amabilis (Jewett, 1958) comb. nov. (Plecoptera, Perlodidae) based on types and new material from Himalaya and Pamir Mountain systems. Zootaxa, 4767 (2), 295 - 306. https: // doi. org / 10.11646 / zootaxa. 4767.2.5","Ozdikmen, H. (2008) A new name for the preoccupied stonefly genus Aubertiana Zhiltzova, 1994 (Plecoptera). Munis Entomology & Zoology, 3 (2), 761 - 762."]}

Filchneria balcarica Balinsky, 1950 Figs. 1–15 Balinsky, 1950: 60, 61, 63, figs 1a–c. Type material contains two females and has been deposited in the Zoological Museum, Warsaw, Poland. Illies, 1966: 359; Zhiltzova, 1971: 1038‒1039, figs. 3 (1‒4); Zwick, 1973: 229, 235; Cherchesova, 2006: 6; Teslenko & Zhiltzova, 2009: 23‒24, figs. 98–101. Diagnosis. The male of F. balcarica is distinguished by a slightly elevated posterior margin of the tergum 10 that is truncated and straight dorsomedially, its posterolateral edges rounded; large pyramidal paraprocts; and a heavily sclerotized paraproctal sclerite with membranous protrusions at the apex and along the inner edge. The eversible paraproct lobe (EPL), as everted from live material, looks like two rounded membranous swellings covered with tiny sensory spines. Females of F. balcarica differ in the large, wide, and bilobed subgenital plate with deep, Ushaped notch; lobes are widely spaced, rounded and directed inward. The egg is unique, large, and has parallel lateral margins before the transverse ridge. The posterior pole is truncated and medially depressed. The collar is almost unnoticeable, its anchor plate flat, pan-shaped, and pulled inward. The chorionic surface is rough with numerous, raised, large rounded tubercles, densely arranged on the longitudinal ridges. The micropyles, located near equatorially, have lipped orifices. Complementary description. Male. The original description of the species was carried out on the female (Balinsky 1950), the male was described later (Zhiltzova 1971). The overall coloration is similar to that of the female, the male significantly smaller than female, body length 14.5–19.7 mm in (n=2); brachypterous, wings not exceeding tergum 3 (Figs. 1–4). Abdomen dark brown, terga 1‒7 with an indistinct longitudinal medial brown band delimited by pair of narrow, pale bands laterally (Figs. 1, 5). Terga 5–8 humped laterally. Tergum 8 with a pale membranous longitudinal stripe medially and two submedial swellings densely covered by sensilla basiconica and colorless hairs posterolaterally. Tergum 9 is half hidden under tergum 8; anterior margin and narrow stripe medially is dark, posterior half with pale swelling, covered by sensilla basiconica and colorless hairs posterolaterally (Fig. 5). In caudal view, posterior margin of tergum 10 is weakly elevated, directed forward and upward; in the dorsal view, the posterior margin truncated and straight medially; the posterolateral edges rounded, covered with oval patches of sensilla basiconica which are connected along posteromedial edge, occupying the posterior half of the tergum 10 length (Figs. 5–8). At rest, the paraproct is pyramid-shaped with a rounded tip, inner plane of paraproct weakly sclerotized (Fig. 7). Paraproctal sclerite large, heavily sclerotized dorsally and basally, except apex and inner edge which bears a membranous notch in the middle (Fig. 7). The EPL enlarges into two rounded membranous swellings covered with tiny sensory spines (Figs. 5, 8). Female. Body length 18.2‒19.5; macropterous (Fig. 1), length of wings 19.3‒20.7 mm (n=2). Sterna 6‒7 are pale with pair of rounded brown spots laterally and brown band anteriorly, slightly widened anterolaterally. Sternum 8 pale with pair of rounded brown spots laterally and a pair of oblique dark brown lateral sclerites which surround subgenital plate anterolaterally (Fig. 9); a paired small brown lateral spots at the base of the subgenital plate closely to posterior margin of sternum 8. Subgenital plate bilobed (Fig. 9), wide and large, occupies 4/5 of the sternum 8 width and half of the sternum 9 lengths, or covers sternum 9 completely. Notch of the subgenital plate is deep and wide, U-shaped arcuate with paired obtuse angles along inner edge distally divides the subgenital plate into two lateral lobes rounded posteriorly and directed inward, one female has darkened lobes. Sternum 9 pale medially, under the lobes of the subgenital plate brownish. Abdominal sternum 10 is pale. Egg. Ultrastructure is species specific. Relatively large, trilateral in cross-section with dimensions of 619×442 μm. Flat longitudinal ridges delimit the three sides of the egg; each side has additionally a transverse ridge close to the anterior pole (Figs. 10, 11). Egg has almost parallel lateral margins before the transverse ridge, posterior pole truncated, straight, and medially depressed (Figs. 10, 11). The collar is unnoticeable, pedicel short and thin (Figs. 10, 12). The anchor plate flat, pan-shaped, is pulled inward and covered with single globular bodies except for the center (Figs. 11–13). Large globular bodies gathered on the shoulder surface around outer edge of the anchor plate (Fig. 12). The chorionic surface is rough with numerous, raised, relatively large rounded tubercles, densely arranged on the longitudinal ridges (Figs. 10, 11, 15). A transverse row of six micropyles located almost equatorial; micropyles have a lipped orifices; the micropyles height is equal to or slightly exceeds the height of the chorionic tubercles (Figs. 10, 11, 14). Material examined. 1 male, Georgia, Svanetia, Enguri River, source, 3.08.1957, coll. LA Zhiltzova (ZIN); 1 male, Georgia, Mentrel Ridge, Tekрuri River, altitude 2300 m above sea level, 1.04.1989, coll. A. Koval; 1 female, Abkhazia, Avadkhara River, a left branch near its headwaters, 14.07.1958, collector unknown (ZIN); 1 female, Russia, North Ossetia, Tseiskiy Ridge, Tseidon River, the left source, higher than the glacier, N 42°77.5297’, E 43°85.568’, 24.07.2021, coll. DMP (VLA). Ecology and distribution. Filchneria balcarica was recently found in the headwaters of glacier-fed stream at 2300 m above sea level, the water temperature being 5°C and the velocity ranging from 0.4‒ 1 m /s. Bottom substrates consisted of cobbles and gravel. This relatively rare species is limited to North-West Caucasus and occurs in Georgia, Abkhazia, Kabardino-Balkaria, and the Republic of North Ossetia-Alania. It inhabits various mountain water courses from rivers to small streams. The emergence period is prolonged, occurring from April-August., Published as part of Teslenko, Valentina A. & Palatov, Dmitry M., 2023, Redescription of a few Filchneria Klapálek, 1908 (Plecoptera, Perlodidae) species on the type and fresh material, pp. 287-312 in Zootaxa 5277 (2) on pages 288-292, DOI: 10.11646/zootaxa.5277.2.3, http://zenodo.org/record/7889837, {"references":["Balinsky, B. (1950) On the Plecoptera of the Caucasus. Transactions of the Royal Entomological Society of London, 101 (2), 59 - 87.","Illies, J. (1966) Katalog der rezenten Plecoptera. Das Tierreich, Berlin, 82, 1 - 632.","Zhiltzova, L. A. (1971) The genus Filchneria Klap. and its position in the family Perlodidae (Plecoptera). Zoological Journal, 50, 1034 - 1040. [in Russian]","Zwick, P. (1973) Insecta: Plecoptera. Phylogenetisches System und Katalog. Das Tierreich 94. Walter de Gruyter, Berlin, xxxii + 465 pp.","Cherchesova, S. K. (2006) Stoneflies (Plecoptera) of Caucasus: history of study and research's perspectives. Russian Entomological Journal, 15 (1), 3 - 8.","Teslenko, V. A. & Zhiltzova, L. A. (2009) Keys to the stoneflies (Insecta, Plecoptera) of Russia and adjacent countries. Imagines and larvae. Dalnauka, Vladivostok, 382 pp. [in Russian]"]}

Isoperla dicala Frison, 1942 (Figs. 41–48) Sable Stripetail http//lsid.speciesfile.org/urn:lsid: Plecoptera.speciesfile.org:TaxonName:468699 Isoperla dicala Frison 1942:321. Holotype male (INHS), Free Soil, Great Sable River, Mason Co., Michigan, U.S.A. Isoperla dicala: Hitchcock 1974:197. Isoperla dicala: Poulton & Stewart 1991:48. Isoperla dicala: Szczytko & Kondratieff 2015:86. Distribution. Canada —MB, NB, NS, ON, PQ. U.S.A. —AL*, AR, CT, GA, FL* IA, IN, KY, MA, MD, ME, MI, MN, MO, MS*, NC, NY, OH, PA, SC, TN, VA, WI, WV (DeWalt et al. 2023). (records with * are considered dubious based on the present study) Material examined. U.S.A. — Michigan: Presque Isle Co., Ocqueoc River, Ocqueoc Falls Hwy parking area, 45.39724, -84.01754, 11 June 2019, S. Beaty, A. Beaty, 1M, 2F (NCDWR). Missouri: Shannon Co., Round Springs, Round Springs State Park, [37.28270, -91.40768], 22 June 1974, J. Morse, 1F (NCDWR). Texas Co., Current River, Montauk State Park, [37.45059, -91.68704], 4 June 1976, B. Stark, J. Lackey, 5M, 9F (INHS, Insect Collection 875889). North Carolina: Alleghany Co., Brush Creek, SR 1444, 36.49416, -81.00472, 3 April 2006, T. MacPherson, 2L (NCDWR). Ashe Co., South Fork New River, US 221, 36.47380, -81.33694, 14 May 1990, D. Penrose, 1L, (NCDWR). Buncombe Co., Flat Creek, SR 1741, 35.72833, -82.61528, 24 April 1986, D. Penrose, 5L, (NCDWR); [Swannanoa River], Swannanoa, [35.59789, -82.39984], 19 June 1938, T.H. Frison, T.H. Frison Jr., 1M (INHS, Insect Collection 875897). Burke Co., Linville River, NC 126, 35.79472, -81.89027, 10 April 1990, L. Eaton, D. Penrose, K. Lynch, 1L, (NCDWR). Caldwell Co., Johns River, SR 1356 (Old Johns River Rd.), 35.93416, -81.69138, 6 April 2019, C. Verdone, S. Beaty, V. Holland, 1F (NCDWR). Caldwell Co., Thorps Creek, Mortimer Campground, 35.99054, -81.76096, 13 April 2012, S. Beaty, V. Holland, E. Fleek, 1L, (NCDWR); Wilson Creek, NC 90, 36.01381, -81.76700, 13 April 2012, S. Beaty, V. Holland, E. Fleek 1L (NCDWR); Yadkin River, near SR 1516, 35.99166, -81.55805, 6 June 2006, M. Walters 1L, (NCDWR); Yadkin River, off SR 1372, 36.06895, -81.58553, 23 May 2011, D. Black, S. Beaty, E. Fleek 1L (NCDWR). Henderson Co., North Fork Mills River, SR 1343, 35.39425, -82.61503, 3 May 2011, T. Mormon, M. Walters, D. Black 1L, (NCDWR); South Fork Mills River, SR 1340, 35.37569, -82.61440, 1 June 2009, T. MacPherson, M. Walters, E. Fleek 2L, (NCDWR); same location, 35.37569, -82.61440, 3 May 2011, T. Mormon, M. Walters, D. Black, 4L, (NCDWR); same location, 25 June 2002, T. Mormon, 1L, (NCDWR). Macon Co., Cullasaja River, off Fox Ridge Rd, 35.17172, -83.36720, 28 May 2019, V. Holland, S. Beaty, C. Verdone, 1L (NCDWR). Rockingham Co., Mayo River, Anglin Mill Rd., 36.53530, - 79.99070, 11 May 2018, C. Verdone, 2M, 1F (NCDWR); same location, 22 April 2020, S. Beaty, V. Holland, C. Verdone, 1F, 1E (NCDWR); same location, 2 May (no year given), D. Lenat, 3L (NCDWR). Surry Co., Fisher River, near SR 1346, 36.41803, -80.71752, 4 May 2010, V. Holland, M. Walters, T. Mormon, 2L (NCDWR). Swain Co., Nantahala River, Hwy 19, 35.33184, -83.60671, 27 May 1994, S.W. Szczytko, J.B. Sandberg, B. Stark, 1M (INHS, Insect Collection 875908). Transylvania Co., French Broad River, SR 1129, 35.14861, -82.79972, 9 April 2019, C. Verdone, S. Beaty, V. Holland, E. Fleek, 10L (NCDWR). Watauga Co., Cove Creek, off SR 1121, 36.23944, -81.82138, 15 May 2007, E. Fleek, T. MacPherson, S. Beaty 2L (NCDWR). Yancey Co., Cane River, off SR 1114, 35.90437, -82.33250, 7 May 2008, D. Black, M. Walters, B. Crouch, 5L (NCDWR); Cane River, US 19E, 35.91222, -82.34944, 14 May 2013, D. Lenat, 1L (NCDWR). South Carolina: Oconee Co., Chattooga River, Hwy 28 at Georgia State Line, [34.91958, -83.16882], 24 May 1993, B. Kondratieff, R. Kirchner, 1M (NCDWR). Pickens Co., South Saluda River, 4 mi N Pumpkintown, [35.06719, -82.61793], 22 June 1969, H. Douglass, 1F (INHS, Insect Collection 875884). Tennessee: Blount Co., Abrams Creek, Abrams Creek Campground, Great Smoky Mountains National Park, 35.61030, -83.93270, 10 June 2001, B.D. Heinold, 2F (INHS, Insect Collection 3491). Sevier Co., Little Pigeon River, Gatlinburg, [35.71417, -83.51028], 12 June 1935, H.H. Ross, 1F (INHS, Plecoptera 2957); Gatlinburg, [35.71426, -83.51016], 11 June 1938, T.H. Frison, T.H. Frison Jr., 1F (INHS, Insect Collection 875854); same location, 14 June 1940, [T.H.] Frison, [C.O. Mohr, A.W. Hawkins] 2F, (INHS, Plecoptera 2956); Sevierville, 35.86806, -83.56194, 11 June 1938 T.H. Frison, T.H. Frison Jr., 5F (INHS, Insect Collection 875863); same data, 3F (INHS, Insect Collection 875856); same data, 1M (INHS, Insect Collection 875892). Virginia: Bland Co., Clear Fork, Hwy 61, 0.5 mi W of Clear Fork, 37.20992, -81.20296, 6 June 2016, C. Verdone, B. Kondratieff, 1F (NCDWR); Wolf Creek, Grapefield Rd., Stephen Levitt Property, 37.14702, -81.26314, 4 June 2016, C. Verdone, B. Kondratieff, 3F (NCDWR); same location, 6 June 2016, C. Verdone, B. Kondratieff, 2F (NCDWR); s ame location, 8 June 2016, C. Verdone, B. Kondratieff, 2F (NCDWR); same location, 23 May 2017, C. Verdone, 3F, 2F (NCDWR); same location, 27 May 2017, C. Verdone, D. Fuller, 1F (NCDWR); Wolf Creek, Grapefield Rd., Wolf Creek Picnic Area, 37.18026, -81.19496, 8 June 2016, C. Verdone, B. Kondratieff, 1F (NCDWR); same location, 7 May 2017, C. Verdone, B. Kondratieff, S. Roble, 1M, 2F (NCDWR); Wolf Creek, Hwy 61, War Memorial, 37.24324, -81.10800, 27 May 2016, C. Verdone, B. Kondratieff, 4F (NCDWR); same location, 8 June 2016, C. Verdone, B. Kondratieff, 1F (NCDWR). Botetourt Co., Craig Creek, Craig Creek Rec Area (2 mi SE of Oriskany), 37.61345, -79.95624, 9 June 2016, C. Verdone, B. Kondratieff, 1F (NCDWR). Craig Co., Potts Creek, Rte 18, 37.59814, - 80.22697, 4 May 2019, C. Verdone, D. Fuller, 1M (NCDWR). Floyd Co., Floyd, [36.91124, -80.32005], no date, no collector data, 3F (VMNH). Franklin Co., Otter Creek, Philpott Reservoir, end of Rte 789, 2.4 mi SE of Endicott, [36.86837, -80.11660], 25 August 2000, A.C. Chazal, 2F (VMNH); Pigg River, 0.3 km upstream of Rte 890 bridge SW Pennook, [36.94638, -79.64052], 26 June 2005, R.L. Hoffman, 1M, 2F (VMNH); UT Nicholas Creek, VA 781, 2.5 mi W Ferrum, 36.91615, -80.06237, 6 July 2000, R.L. Hoffman, 1F (VMNH). Giles Co., Sinking Creek, at VA 700 Bridge, 37.31149, -80.51610, 3 July 1992, R.L. Hoffman, 3F (VMNH). Grayson Co., New River, Hwy 58 Boat Access, 36.61340, -81.04464, 29 May 2016, C. Verdone, B. Kondratieff, 1F (NCDWR), same location, 11 May 2017, C. Verdone, B. Kondratieff, 1F (NCDWR). Henry Co., South Mayo River, at VA 695, ca 5 mi SSW of Spencer, [36.55577, -80.02128], 6 June 1990, R.L. Hoffman, 2F (VMNH); Toeclout Branch, at VA 637, 3 mi SE of Ridgeway, [36.54852, -79.84263], 12 May 1990, R.L. Hoffman, 1M, 2F (VMNH). Patrick Co., Dan River, Rte 648, 36.62230, -80.44497, 11 May 2017, C. Verdone, B. Kondratieff, 2M (NCDWR). Pittsylvania Co., Crooked Run, VA 841 6 mi SW Callands, [36.74825, -79.65121], 9 June 2000, R.L. Hoffman, 1F (VMNH); Dan River, VA 860 bridge, ca 1 mi SE of Cascade, [36.54417, -79.66183], 31 May 1989, R.L. Hoffman, 1M (VMNH); Sandy River, Rte 845 bridge 4.5 mi N Brosville, [36.67935, -79.59389], 7 June 1993, R.L. Hoffman, 1F (VMNH). Prince William Co./ Fairfax Co., Bull Run, Manassas National Battle Field, [38.82435, -77.50375], 30 July 1997, C.S. Hobson, 1F (VMNH). Pulaski Co., Little Reed Island Creek, Rte 607 at Boone Furnace, [36.92150, -80.76976], 10 June 1990, R.L. Hoffman, 1F (VMNH). Russell Co., Clinch River, Rte 798 SE of Rte 645 nr Nash Ford, 36.96549, -82.07749, 24 May 2017, C. Verdone, 3M, 4F (NCDWR). Scott Co., Clinch River, Rte 65, Dungannon Boat Access, 36.83108, -82.46252, 6 May 2017, C. Verdone, B. Kondratieff, S. Roble, 3M, 6F (NCDWR). Smyth Co., North Fork Holston River, Carl Slate Memorial Boat Landing, 36.89335, -81.75539, 5 May 2017, C. Verdone, B. Kondratieff, S. Roble, 5M, 5F (NCDWR); South Fork Holston River, jct. Riverside Rd./ Chestnut Ridge Rd., 36.76200, -81.63585, 8 May 2017, C. Verdone, B. Kondratieff, 8M, 3F (NCDWR). Tazewell Co., spring pond on Smith Ridge, 1 mi N of Pounding Mill, [37.09095, -81.71416], 4 June 1999, Rodney Cordle, 1M, 2F (VMNH). Washington Co., Wolf Creek, St. Rte 80 nr Conflu w/ North Fork Holston River, 36.82404, -81.92167, 4 May 2017, C. Verdone, B. Kondratieff, 1M (NCDWR). Adult male. Macropterous; forewing length 7.5–9.3 mm (n = 16), body length 6.9–8.9 (n = 16). Head pale yellow with brown bands connecting lateral ocelli to anterior ocellus. General body color pale yellow in life with few brown markings. Dorsum of Head (Fig. 16). Palpi pale yellow-brown. Labrum with stiff, short setae. Dorsum of head pale yellow with brown bands connecting median ocellus with lateral ocelli; interocellar area pale, open posteriorly; frons generally pale yellow. Antennal scape and pedicel yellow; flagellum brown with basal 7–8 segments pale, antennal length about ½ length of body with 32 segments. Thorax. Pronotum with a wide, pale median stripe which extends to anterior margin (Fig. 16); rugose area laterad of median stripe pale brown; pigment extends anteriorly to pronotal margin; rugose area with short stout brown setae interspersed; posterior edge of pronotum pale; lateral pronotal margins pale. Mesonota and metanota yellow with yellow-brown scutal humps; nota with sparse, short, fine pale setae. Wings hyaline with light brown veins (Fig. 26). Mesobasisterna and metabasisterna pale yellow. Legs. Anterior and posterior faces of femora pale yellow. Tibiae yellow with a thin transverse dark brown band near proximal ¼. Tarsi light brown, segment 3 darker apically. Leg vestiture dense with short dark setae giving legs a darker overall appearance; tibiae with widely spaced subdorsal and ventral rows of larger spines. Abdomen. Terga pale yellow and clothed with short, fine golden setae. Sterna pale yellow. Sterna 6–9 with a posterior fringe of short, brush-like, brown setae. Sternum 8 with a deeply recessed, pale brown vesicle (Fig. 17) 2.1–3.0X (n = 14) longer than wide when measured from apex to anterior sclerite / maximum subapical width; apex slightly expanded, evenly rounded, extending to base of segment 9. Paraprocts triangular, minimally sclerotized, pale yellow; apices narrow and acutely rounded; not recurved over tergum 10 (Fig. 18). Cerci pale yellow; cercal length ½ as long as abdomen, with 12 segments, each segment with one long, light brown ventral seta near posterior margin, basal 4–6 segments with dorsal and ventral tufts of long stiff setae, setae becoming shorter and less numerous on each successive segment. Aedeagus (Figs. 19–25). Male appendage with a short, broad basal stalk, posteroventral lobe, posterior lobe, pair of small caudal lobes, conical apical lobe and paired hemispherical anterior lobes (Figs. 19, 22). Paired caudal lobes (Figs. 19–22), small, often difficult to discern. Conical apical lobe (Figs. 19, 22) often depressed apically, flaplike, appearing triangular in dorsal and ventral aspects (Figs. 20–21); apex broadly rounded. Anterior hemispherical lobes membranous (Figs. 19–22). Aedeagal armature (Figs. 22–25). Basal stalk encircled with short stout spinulae (Fig. 22); posteroventral lobe with sharp spinulae which transitions to dense hair-like spinulae dorsally (Fig. 24). Mediolateral areas of aedeagus also covered in sharp spinulae (Fig. 22). Posterior lobe with small mounds each adorned with a cluster of small sharp spinulae (Fig. 25). Area dorsolateral to paired caudal lobes with linear brushes of hair-like spinulae and sensilla basiconica which transition to small, rounded knobs with apical filaments anteriorly (Fig. 23). Adult female (Fig. 26). Macropterous; forewing length 8.1–10.1 mm (n = 11). Body length 7.9–10.3 (n = 11). General head pattern and body color similar to that of the male. Posterior margin of tergum 7 and posterolateral margins of terga 8–9 bearing brush-like setae. Subgenital plate (Fig. 27). Pale yellow, broadly triangular and produced posteriorly approximately ⅛–¼ the length of sternum 9; plate basally broad, about ¾ the width of segment 8 on most specimens, apex obtuse; plate with scattered short golden setae and a few longer setae interspersed. Ovum (Figs. 28–31). Length 268.7–277.4 μm (n = 2); width 200.3–202.2μm (n = 2). Color pale yellow. General shape oblong; cross section concave; chorion covered with subtle reticulate ridges; collar well-developed (Figs. 28–29). Follicle cell impressions hexagonal or pentagonal, weakly developed and with clusters of globules at their center (Figs. 30–31). Eclosion line weakly developed, micropyles positioned on eclosion line, spaced regularly around egg circumference (Figs. 31). Collar height 28.7–34.3 μm, width 56.2–56.9 μm (n = 2); width either subequal or slightly narrowed from base to apex; densely covered in globules (Figs. 28–29). Mature larva (Figs. 32–35). Pre-emergent larvae 6.7–9.2 mm (n = 17). Body with lightly contrasting body pattern. Dorsum of head (Fig. 33). Mottled brown with pale medial area anterior to median ocellus, a pair of large anterolateral pale spots, a pair of mediolateral pale spots, a small enclosed pale ocellar spot, and pale ocular areas. Anterior frontoclypeus with a dark bar that is obliterated medially by medial extension of pale median area. Pale medial area often shaped like a spinning top; sometimes with a short anteromedial extension and with small anterolateral projections, resembling a duck foot overall. Anterolateral pale spots variable and irregularly shaped, usually distinctly separated from pale medial area. Pale medial spot and anterolateral spot bordered by a dark M-line on posterior margins; pale ocellar spot small, usually round, fully enclosed and centered equidistant from ocelli; mediolateral pale spots subquadrate, extending anteromedially from epicranial suture but not reaching lateral ocelli; closed anterior to suture, confluent with pale ocular area. Pale ocular spots extend posteromedially towards posterior occipital margin; pale area divided by a thin angulate line of brown pigment and spicules. Epicranial stem with a pale oval spot covering posterior 2/3. Brown areas of occipital area with clusters of spicules. Clothing hairs on head lightly colored. Antennae yellow; apical segments slightly darker; scape and pedicel yellow to yellow-brown; flagellum with 30–39 antennomeres (n=13); each segment with an apical circlet of small spines. Maxilla (Figs. 36–37). Lacinia bidentate; receding evenly from base to subapical tooth; a thin, marginal seta ¾ subapical tooth length, proximal to subapical tooth; 5–6 (rarely 7) striated, stout marginal setae below subapical tooth, last few setae progressively smaller; 8–10 thin, unevenly-spaced setae along palm edge and curving dorsad; a submarginal row of 3–4 striated stout setae below apical tooth; ventral face of palm basally with 5–7 setae. Length of striated apical tooth of lacinia between 0.5–0.6X palm length and 0.6–0.7X the palm width. Subapical tooth 0.5–0.6X the length of the apical tooth. Galea 1.0–1.1X the lacinial palm length, with a ventral row of less than 30 setae and tipped with 2–4 apical spinous setae. Maxillary palp setose, 1.4–1.5X length of lacinia; segments 1–4 successively longer, segment 4 slightly longer than 5; segments 1 and 2 with transverse row of apical spinous setae; segment 3 sometimes with one apical spine, segment 5 tipped with 1–2 sensillae. Mandibles (Figs. 38–39). Right mandible (Fig. 38) bicuspid, outer cusp with three teeth, ventral tooth largest and serrated basally, dorsal tooth smallest; a band of unorganized, long spinous setae on ventral surface from base of outer ventral tooth to base of mandible; inner cusp with three teeth, middle tooth longest and largest; a few sharply pointed acanthae from basal tooth on inner cusp to molar ridge (Fig. 38 inset); molar ridge with a dense row of spine-like setae, setae longer and thinner towards base of mandible; dorsal surface of mandible with a band of unorganized setae from inner cusp towards base of mandible. Left mandible (Fig. 39) similar to right except acanthae at base of inner cusp replaced by a dense brush of short spinous setae (Fig. 39 inset). Thoracic nota (Figs 32–33). Pronotum (Figs. 32–33) brown with contrasting pattern. Medial stripe yellow-brown, wider both anteriorly and posteriorly; medially interrupted on both anterior and posterior margins by brown triangular markings. Anterior pronotal flange with alternating stripes of pale and dark pigment; lateral edges pale, with sublateral brown stripes. Pronotal disk with an irregular pale crescent enclosed by brown markings. Posterior pronotal flange similar to anterior flange. Pronotum rimmed with short, closely set, stiff setae, intervening long setae mostly absent except at pronotal corners. Mesonota and metanota with irregular brown markings; wing pads yellow-brown with three sublateral longitudinal brown stripes (Fig. 32). Lateral margins of nota fringed with a short, closely set row of stiff setae. All thoracic nota with sparse, short, pale to brown clothing hairs. Thoracic sterna pale, without distinctive markings; furcal pits and Y-stem slightly darker in most specimens. Clothing hairs on sternum pale, sparse. Legs: Femora yellow to yellow-brown; ventral face mostly pale with a few stout, golden spines; anterior face yellow with an obscure submedial yellow-brown longitudinal band and regularly spaced stout golden setae; dorsally with a sparse fringe of silky setae. Tibiae pale yellow-brown with a broad ventral row of stout setae; anterior face with two longitudinal rows of stout setae separated by a row of long silky setae, setae twice as long as width of tibia. Tarsi pale yellow-brown with a ventral row of stiff setae and a sparse dorsal row of silky setae. Two moderately long claws darker apically, gradually curved. Abdomen (Figs. 32, 34–35). Terga (Figs. 32, 34) with three dark brown longitudinal stripes bordered by pale lines, with intervening stripes wider and paler; in lateral aspect, anterior half of each segment with a pair of small, variably dark dots sometimes overlaid by a coalescing stripe on distal segments. Terga with numerous spicules with dark origins; posterior edges of abdominal segments bearing a transverse row of stout spines, longer spines interspersed among 2–3 shorter spines; Sterna (Fig. 35) pale and with spicules with dark origins typically restricted to segments 7–10; spicules may be present on anterior segments, but rarely have dark origins; posterior edges of sterna bearing a transverse row of long clear spines; female larvae with row incomplete medially on tergum 8. Cerci yellow-brown with 18–22 segments (n = 13), each with an apical circlet of short spines; cerci with sparse dorsal fringe of silky setae on distal half. Diagnosis. Isoperla riverae sp. n., Published as part of Verdone, Chris J., Beaty, Steven R., Holland, Victor B. & Williams, Bronwyn W., 2023, Isoperla riverae, a new stonefly species from the southeast Nearctic, with notes on sympatric species including the larval description of Isoperla lenati Szczytko & Kondratieff, 2015 (Plecoptera: Perlodidae), pp. 437-470 in Zootaxa 5270 (3) on pages 457-468, DOI: 10.11646/zootaxa.5270.3.3, http://zenodo.org/record/7860146, {"references":["Frison, T. H. (1942) Studies of the North American Plecoptera, with special reference to the fauna of Illinois. Bulletin of the Illinois Natural History Survey, 22, 235 - 355. [https: // www. ideals. illinois. edu / items / 44818] https: // doi. org / 10.21900 / j. inhs. v 22.245","Hitchcock, S. W. (1974) Guide to the insects of Connecticut. Part VII. The Plecoptera or stoneflies of Connecticut. State Geological and Natural History Survey of Connecticut Bulletin, 107, 1 - 262. [https: // files. nc. gov / ncdeq / Water % 20 Quality / Environmental % 20 Sciences / BAU / Benthos % 20 Reference / Plecoptera % 20 of % 20 Connecticut % 201974. pdf]","Poulton, B. C. & Stewart, K. W. (1991) The stoneflies of the Ozark and Ouachita Mountains (Plecoptera). Memoirs of the American Entomological Society, 38, 1 - 116. [https: // www. biodiversitylibrary. org / page / 38911829 # page / 11 / mode / 1 up]","Szczytko, S. W. & Kondratieff, B. C. (2015) A review of the eastern Nearctic Isoperlinae (Plecoptera: Perlodidae) with the description of twenty-two new species. Monographs of Illiesia, No. 1, 1 - 289. [http: // illiesia. speciesfile. org / papers / Monographiae-of-Illiesia. pdf]","DeWalt, R. E., Maehr, M. D., Hopkins, H., Neu-Becker, U. & Stueber, G. (2023) Plecoptera Species File Online. Version 5.0 / 5.0. Available from: http: // Plecoptera. SpeciesFile. org / (accessed 26 January 2023)","Beaty, S. R., Holland, V. B. & Lenat, D. R. (2017) Isoperla arcana and Isoperla borisi (Plecoptera: Perlodidae), two new stonefly species from North Carolina, U. S. A. with notes on the distribution of Isoperla powhatan. Illiesia, 13 (14), 140 - 166. [http: // illiesia. speciesfile. org / papers / Illiesia 13 - 14. pdf]","Verdone, C. J. & Kondratieff, B. (2016) A new species Isoperla Banks (Plecoptera: Perlodidae) from the Appalachian Mountains, Virginia & West Virginia, U. S. A. Illiesia, 12 (13), 74 - 85. [http: // illiesia. speciesfile. org / papers / Illiesia 12 - 13. pdf]","Verdone, C. J. & Kondratieff, B. C. (2017) A new species of Isoperla Banks (Plecoptera: Perlodidae) from the southern Appalachians, with notes on the I. montana group. Illiesia, 13 (12), 111 - 126. [http: // illiesia. speciesfile. org / papers / Illiesia 13 - 12. pdf]","Stark, B. P. & Gaufin, A. R. (1978) The stoneflies (Plecoptera) of Florida. Transactions of the American Entomological Society, 104, 391 - 433. [https: // www. jstor. org / stable / 25078229]","Stark, B. P. & Harris, S. C. (1986) Records of stoneflies (Plecoptera) in Alabama. Entomological News, 97, 177 - 182. [https: // www. biodiversitylibrary. org / page / 2723576 # page / 443 /]","Lenat, D. R. (1993) A biotic index for the southeastern United States: derivation and list of tolerance values, with criteria for assigning water quality ratings. Journal of the North American Benthological Society, 12 (3), 279 - 290. https: // doi. org / 10.2307 / 1467463"]}

Isoperla riverae sp. n. Verdone, Beaty, Holland & Williams (Figs. 16–39) Isoperla dicala: Stark & Gaufin, 1978:401 Isoperla dicala: Szczytko & Kondratieff, 2015:88 (in part) Etymology. The new species is named for the senior author’s daughter. May she enjoy the splendor of the natural world and value all its inhabitants. The proposed common name is “River’s Stripetail”. Distribution. U.S.A. —AL, FL, GA, NC, MS, SC, TN, VA (Fig. 15) Material examined. Holotype M: U.S.A. — North Carolina: Moore / Richmond Co., Drowning Creek, SR 1102, 35.08426, -79.58270, 28 April 2019, emerged 6 May 2019, C. Verdone, S. Beaty (NMNH). Paratypes: Georgia: DeKalb Co., Snapfinger Creek, 5 mi SE of Panthersville, [33.66552, -84.21116], 4 June 1945, P.W. Fattig, 9M (INHS, Insect Collection 875867). Gwinnett Co., Ivy Creek, 5 mi NS Buford, [34.04788, -84.03133], 11 June 1945, P.W. Fattig, 2M (INHS, Insect Collection 875931). North Carolina: Caldwell Co., Johns River, Old Johns River Rd., [35.93359, -81.69053], 17 May 2004, B. Kondratieff, D. Lenat, R. F. Kirchner, R. Zuellig, 1M (NCDWR); same location, 20 May 2019, C. Verdone, S. Beaty, V. Holland, 1M (NCDWR). Henderson Co., French Broad River, Hwy 64, [35.32853, -82.57524], 23 May 1990, B. Kondratieff, R. F. Kirchner, 5M, 3F (NMNH). Moore Co., Drowning Creek, SR 1004, 35.06575, -79.54998, 28 April 2022, emerged 4 May 2022, C. Verdone, S. Beaty, V. Holland, 1M (NCDWR). Moore / Richmond Co., Drowning Creek, SR 1102, 35.08426, -79.5827, 19 May 2018, C. Verdone, 2M, 61F (NCDWR); same location, 28 April 2019, emerged 6 May 2019, C. Verdone, S. Beaty, 1M, 1E (NCDWR); same location, 28 April 2019, emerged 6 May 2019, C. Verdone, S. Beaty, 2M (NCDWR); same location, 28 April 2019, emerged 13 May 2019, C. Verdone, S. Beaty, 1M, 1E (NCDWR). same location, 28 April 2019, emerged 13 May 2019, C. Verdone, S. Beaty, 1F (NCDWR). Richmond Co., Naked Creek, SR 1003, 35.08197, -79.58909, 27 April 2012, emerged 30 April 2012, D. Lenat, 1F,1E (NCDWR); same location, 27 April 2012, emerged 5 May 2012, D. Lenat, 2F, 2E (NCDWR); same location, 28 April 2019, C. Verdone. S. Beaty, 1F, 1E, 3L (NCDWR); same location, 30 April 2019, C. Verdone, S. Beaty, 1F, 1E (NCDWR); same location, 9 May 2020, C. Verdone, V. Holland, 1M, 1F (NCDWR). Rockingham Co., Mayo River, SR 1358, 36.53483, -79.99086, 11 May 2018, C. Verdone, S. Beaty, V. Holland, 1M (NCDWR); same location, 15 May 2018, C. Verdone, S. Beaty, V. Holland, L. Housley, 2M (NCDWR); same location, 21 May 2019, V. Holland, 1M (NCDWR). South Carolina: Aiken Co., Upper Three Runs, Savannah River Site Rd. C, [33.28518, -81.69533], 5 May 1984, B. Kondratieff, 29M, 8F (NCDWR). Virginia: Franklin Co., Big Chestnut Creek, Rte 718 E, [36.87630, -79.86254], 21 May 2002, B. Kondratieff, R. F. Kirchner, 2M (NMNH). Additional material examined. U.S.A. — Alabama: Clarke Co., Fishers Creek, nr Whatley, [31.63982, - 87.71937], 8 May 1986, S. Harris, P. O’Neil, 44M, 11F (NCDWR). Cleburne Co., Shoal Creek, Shoal Creek Campground, [33.77071, -85.55572], 25 May 1984, S. Harris, P. Lago, 4M, 2F (NCDWR); South Fork Terrapin Creek, Hwy 55, [33.86017, -85.52440], 25 May 1984, S. Harris, P. Lago, 3M, 1F (NCDWR). Monroe Co., Little River, Hwy 59, [31.29888, -87.71048], 11 May 1982, S. Harris, 1M (NCDWR). Randolph Co., Cutnose Creek, Hwy 48, [33.42541, -85.37436], 20 May 1984, S. Harris, P. Lago, 1M, 2F (NCDWR). Tallapoosa Co., Josie Leg Creek, off Hwy 22, [32.98634, -85.85926], 19 June 1983, S. Harris, P. Lago, 1M (NCDWR). Florida: Escambia Co., Canoe Creek, above Bratt Rd., [30.95664, -87.34758], 24 April 2009, D. Ray, 1M (NCDWR). Walton Co., Alaqua Creek, Base Rd. 201, Eglin Air Force Base, [30.61667, -86.16444], 17 April 2006, R. W. Flowers, M.L. Pescador, A.K. Rasmussen, B.A. Richard, 10M (NCDWR). Georgia: Bartow Co., Clear Creek, 6 mi N Cartersville, [34.27560, -84.87250], 3 June 1939, P.W. Fattig, 1M (INHS, Insect collection 875885); Pettit Creek, 2 mi N Cartersville, [34.22127, -84.80251], 3 June 1939, P.W. Fattig, 2F (INHS, Insect Collection 875855). Fulton Co., Nancy Creek, 8 mi N Atlanta, [33.86331, -84.40475], 26 June 1945, P.W. Fattig, 3F (INHS, Insect Collection 875853). Hall Co., no waterbody data, Gainesville, [34.29778, -83.82417], 9 May 1944, T. H. Frison, H.H. Ross, 1F (INHS, Insect Collection 875862). Screven Co., Brier Creek, 7 mi NE Sylvania, [32.83172, -81.56612], D.C. Scott, 1M (INHS, Insect Collection 875895). Union Co., Wolf Creek, 3.5 mi N Neel [s] Gap, [34.78515, -83.90730], 30 May 1943, P.W. Fattig, 3M (INHS, Insect Collection 875891); same location, 8 June 1945, P.W. Fattig, 5M (INHS, Insect Collection 875875). Mississippi: Clarke Co., Moore Mill Creek, Clarkco State Park, [32.10818, -88.69334], 26 May 1990, Tanner, Parham, 1F (NCDWR). Lamar Co., Sandy Run, Okahola Rd., [31.20737, -89.38775], 13 May 1992, B. Stark, M. Hicks, 2F (NCDWR). Pike Co., Tangipahoa River, 3 mi Magnolia, [31.11275, -90.47943], 25 March 1977, B. Stark, 2M, 1F (NCDWR). Simpson Co., Mill Creek, Bushtowns Shiver Rd., [31.84672, - 89.98869], 12 May 1992, B. Stark, E. Stark, C. Bishop, 1M, 5F (NCDWR); Mill Creek, Hwy 472, [31.86365, - 89.99195], 3 May 1990, B. Stark, 2M, 5F (NCDWR); Strong River, 3 mi W of Pinola, Hwy 28, [31.88622, - 89.99349], 22 June 1988, S. Szczytko, 1M (NCDWR); Westville Creek, Jake Barnes Rd. blw. Hale Branch jct., [31.87234, -89.91891], 13 April 1991, B. Stark, 6M, 3F (NCDWR). Smith Co., Clear Creek, 3 mi SW Mize [31.84949, -89.59955], 24 April 1991, B. Stark, D. Tanner, 12F (NCDWR). North Carolina: Alleghany Co., Glade Creek, SR 1422, nr mouth, 36.50000, -81.03639, 20 June 2008, S. Beaty 15L (NCDWR). Ashe Co., Cranberry Creek, SR 1609, 36.4028, -81.29833, 13 May 1998, D. Lenat, 1L (NCDWR); Peak Creek, Off SR 1599, 350M upstream Ore Knob Branch, 36.42083, -81.31944, 15 April 1991, D. Penrose, 1L (NCDWR); Cranberry Creek, SR 1609, 36.44028, -81.29833, 13 May 1998. D. Lenat, 1L (NCDWR). Buncombe Co., South Hominy Cr, Davis Creek Rd., 35.47831, -82.75003, 2 June 2010, V. Holland, 1L (NCDWR); South Hominy Creek, Dale Pen Rd., 35.48817, -82.74259, 1 June 2010, V. Holland, 1L (NCDWR); [Swannanoa River], Swannanoa, 35.59789, - 82.39984], 19 June 1938, T. H. Frison, T. H. Frison Jr., 2M (INHS, Insect Collection 875897); same data, 2M (INHS, Insect Collection 875851). Burke Co., Black Fox Cr, SR 1917, 35.66417, -81.63278. 19 April 1989, T. McPherson, 15L (NCDWR); Henry Fork, NC 18, 35.67361, -81.53611, 20 April 1988, D. Lenat, 25L (NCDWR); Henry Fork, SR 1803, 35.68944, -81.45028, 1 May 2006, B. Crouch, 15L (NCDWR); Henry Fork SR 1854, 35.69556, -81.42722, 1 May 2006, B. Crouch, 16L (NCDWR); Henry Fork, SR 1916, 35.66167, -81.60361, 2 May 2006, B. Crouch, 10L (NCDWR); Henry Fork, SR 1922, 35.66167, -81.63694, 19 April 1988, T. McPherson, 15L (NCDWR); same location, 2 May 2006, T. Mormon, 11L (NCDWR); Ivy Creek, SR 1919, 35.64111, -81.66167, 19 April 1988, D. Lenat, 1L (NCDWR); Jacob Fork, SR 1924, 35.59056, -81.56722, 2 May 2006, B. Crouch, 6L (NCDWR); Little Fork, FSR 228, 35.91056, -81.81833, 19 May 2015, M. Walters, 1L (NCDWR); Rock Creek, SR 1915, 35.64167, -81.55750, 19 April 1988, D. Lenat, 2L (NCDWR); Steels Creek, at FR 228, [35.912264, -81.838327] 25 June 1988, S. Dunkle, 1M (NCDWR); Upper Creek, FSR 982 upstream of Timbered Br, 35.92000, -81.79306, 13 June 1994, D. Penrose, 1L (NCDWR). Caldwell Co., Abingdon Creek, SR 1315, 35.91344, -81.60104, 18 April 2011, T. Mormon, 1L (NCDWR); Yadkin River, off SR 1372 downstream of Jackson Camp Creek, 36.06895, -81.58553. 23 May 2011, D. Black, E. Fleek, S. Beaty, 2L (NCDWR). Catawba Co., Henry Fork, SR 1124, 35.68417, -81.40278. 1 May 2006, B. Crouch, 16L (NCDWR). Cleveland Co., Brushy Creek, SR 1323, 35.33972, -81.61580, 16 May 1995, N. Guthrie, 1L (NCDWR). Davidson Co., Abbott Creek, SR 1735, 36.00833, -80.0775, 10 May 2006, D. Black, S. Beaty, B. Prusha, 4L (NCDWR); Brushy Fork. SR 1810, 35.92444, -80.18028, 14 May 2003, T. Mormon, 9L (NCDWR). Franklin Co., Sandy Creek, US 401, 36.24806, -78.25833, 3 May 1988, T. McPherson, 3L (NCDWR). Gaston Co., Beaverdam Creek, SR 1609, 35.40444, -81.24528, 5 May 2006, B. Crouch, B. Prusha, M. Walters, 15L (NCDWR); Hoyle Creek, SR 1836, 35.33472, -81.13389, 4 May 2006, B. Crouch, 2L (NCDWR); Long Creek, SR 1405, 35.30194, -81.31333, 4 April 1994, N. Guthrie, 1L (NCDWR); same location, 18 April 1995, D. Penrose, 1L (NCDWR); Long Creek, NC 275, 35.30222, -81.22278, 5 April 1991, L. Eaton, 1L (NCDWR); same location, 1 April 1992, D. Lenat, 1L (NCDWR); same location, 19 April 1995, D. Penrose, 1L (NCDWR); Long Creek, NC 274, 35.30778, -81.30333, 4 April 1994, D. Penrose, 1L (NCDWR); same location, 18 April 1995, D. Penrose, 2L (NCDWR); Long Creek, SR 1448, 35.31222, -81.26472, 4 April 1994, K. Herring, 1L (NCDWR), Long Creek, SR 1446, 35.31639, -81.27194, 5 April 1994, D. Penrose, 2L, (NCDWR); UT Long Creek, SR 1446, 35.31750, - 81.27194, 5 April 1994, N. Guthrie, 1L (NCDWR). Graham Co., Little Snowbird Creek, off SR 1115, 35.28697, -83.87841, 4 June 2014, M. Walters, 1L (NCDWR). Henderson Co., Mills River, SR 1353, 35.38778, -82.54611, 2 May 2011, T. Mormon, 2L (NCDWR); same location, 23 April 2012, E. Fleek, 1L (NCDWR); Mills River, NC 191 /280, 35.39139, -82.56806, 2 June 2009, T. Mormon, E. Fleek, M. Walters, 4L (NCDWR); North Fork Mills River, SR 1343, 35.39425, -82.61503, 3 May 2011, T. Mormon, 1L (NCDWR); South Fork Mills River, SR 1340, 35.37569, -82.6144, 1 June 2009, T. Mormon, E. Fleek, M. Walters, 1L (NCDWR). Iredell Co., Olin Creek, SR 1891, 35.94472, -80.84444, 9 May 2006, D. Black, B. Prusha, S. Beaty, 3L (NCDWR); Patterson Creek, SR 1890, 35.92417, -80.82417, 4 April 2006, E. Fleek, 2L (NCDWR); Rocky Creek, SR 1854, 35.99139, -80.85944, 5 June 2003, D. Lenat, 4L (NCDWR); Third Creek, SR 2318, 35.73889, -80.84444, 21 June 1989, D. Lenat, 1L (NCDWR); Third Creek, SR 1521, 35.80917, -81.00222, 8 May 2006, S. Beaty, D. Black, 1L (NCDWR). Jackson Co., [Savannah Creek] Fort Tatham Campground, [US 441], [35.29354, -83.26746], 8 July 1981, B.C. Kondratieff, 1F (INHS, Insect Collection 875913). Lincoln Co., Howards Creek, SR 1200, 35.49833, -81.34056, 3 May 2006, B. Crouch, 2L (NCDWR); Killian Creek, SR 1511, 35.41583, -81.02889, 8 June 1994, D. Lenat, 2L (NCDWR); Leepers Creek, SR 1354, 35.49861, -81.14619, 8 May 2006, B. Prusha, 4L (NCDWR); Pott Creek, SR 1217, 35.55194, -81.31889, 3 May 2006, T. Mormon, 1L (NCDWR). Macon Co., Burningtown Creek, Tellico Rd, 35.28277, -83.48240, 6 May 2008, T. Mormon, 1L (NCDWR); Caler Fork, off Ruby Mine Rd, 35.26860, -83.38656, 7 May 2008, R. Thorpe, 1L (NCDWR); Coon Creek, US 23/441, 35.23033, -83.34921, 9 May 2008, T. Mormon, 1L (NCDWR); Cowee Creek, Leatherman Gap Rd, 35.27046, -83.39047, 8 May 2008, R. Thorpe, 3L (NCDWR); Cowee Creek, NC 28, 35.2625, -83.40944, 23 May 2007, D. Black, 1L (NCDWR); Downes Branch, SR 1392, 35.21263, -83.49833, 28 April 2011, V. Holland, 1L (NCDWR); Iotla Branch, SR 1387, 35.22425, -83.42923, 7 May 2008, R. Thorpe, 3L (NCDWR); Iotla Creek, SR 1385, 35.21887, -83.42293, 7 May 2008, C. Tyndall, 2L (NCDWR); Iotla Creek, off SR 1378, 35.23473, -83.39824, 24 May 2007, D. Black, 2L (NCDWR); same location, 7 May 2008, T. Mormon, 1L (NCDWR); Mica City Creek, SR 1346, 35.2696, -83.37045, 25 April 2011, V. Holland, 3L (NCDWR); North Fork Coweeta Creek, SR 1114, 35.06525, -83.40253, 17 May 2011, V. Holland, 2L (NCDWR); Rabbit Creek, off SR 1504, 35.20690, -83.34836, 8 May 2008, R. Thorpe, 12L (NCDWR); Rabbit Creek, SR 1504, 35.20837, -83.35209, 8 May 2008, T. Mormon, 3L (NCDWR); Younce Creek, Younce Creek Rd., 35.23775, -83.47404, 26 April 2011, V. Holland, E. Fleek, S. Beaty, 1L (NCDWR). McDowell Co., Catawba River, SR 1273, 35.61944, -82.18833, 18 April 1985, NCDWR, 1L (NCDWR); Catawba River, SR 1234, 35.63694, -82.14389, 18 April 1985, NCDWR, 1L (NCDWR); North Muddy Creek, SR 1760, 35.67528, -81.90639, 17 April 1985, NCDWR, 16L (NCDWR); Youngs Fork, SR 1819, 35.65389, -81.96306, 17 April 1985, NCDWR, 1L (NCDWR). Nash Co., Sandy Creek, SR 1405, 36.12694, -78.02472, 21 April 2003, E. Fleek, 3L (NCDWR); Swift Creek, SR 1310, 36.11167, -77.92111, 24 April 2003, E. Fleek, 2L (NCDWR). Polk Co., Rotten Creek, off Jeep Trail 35.34709, -82.21117, 16 April 2014, V. Holland, 1L (NCDWR); Whiteoak Creek, SR 1 352, 35.26055, -82.00388, 15 May 1995, N. Guthrie, L. Eaton, K. Herring, 1L (NCDWR). Richmond Co., Naked Creek, SR 1003, 35.08194, -79.59028, 6 April 1990, L. Eaton, 2L (NCDWR); Naked Creek, SR 1003, 35.08197, -79.58909, 18 April 2013, D. Lenat, 1L (NCDWR); Rocky Ford Branch, SR 1424, 35.12417, -79.66250, 9 May 1990, D. Lenat, 8L (NCDWR). Rockingham Co., Falls Creek, Mayo River State Park, 36.539178, -79.988084, 1 May 2012, D. Lenat, 1L (NCDWR); Rock House Creek, SR 2127, 36.42083, -79.79055, 12 April 2001, L. Eaton, 1L (NCDWR). Rutherford Co., Second Broad River, above Union Mills, 35.47917, -81.94417, 19 May 1999, K. Herring, 4L (NCDWR); Second Broad River, below Union Mills, 35.47667, -81.94250, 19 May 1999, K. Herring, 1L (NCDWR) Second Broad River, SR 1504, 35.48401, - 81.94921, 18 May 2010, D. Black, S. Beaty, E. Fleek, 1L (NCDWR). Stokes Co., Brushy Fork, SR 1998, 36.32306, -80.30278, 18 May 2004, N. Flint, 1L (NCDWR); East Prong Little Yadkin River, SR 1224, 36.33126, -80.38586, 17 May 1994, N. Guthrie, 4L (NCDWR); Little Yadkin River, SR 1104, 36.29889, -80.41389, 18 May 1994, D. Penrose, 1L (NCDWR); same location 31 May 1989, D. Penrose, 1L (NCDWR); Little Yadkin River, SR 1104, 36.29899, -80.41388, 6 May 1987, D. Penrose, 2L (NCDWR); same location, 31 May 1989, D. Penrose, 1L (NCDWR). Surry Co., Endicott Creek, Raven Knob Rd., 36.47274, -80.85152, 5 May 2010, V. Holland, M. Walters, 2L (NCDWR); Fisher River, nr SR 1346, 36.41806, -80.71752, 4 May 2010, V. Holland, M. Walters, 4L (NCDWR). Transylvania Co., Catheys Creek, SR 1338, 35.20750, -82.78056, 21 May 1987, D. Penrose, 1L (NCDWR). Wilkes Co., Elk Creek, SR 1175, 36.10639, -81.43808, 7 June 2006, M. Walters, 1L (NCDWR); Left Prong Stony Fork, off SR 1155, 36.15065, -81.45392, 20 April 2012, S. Beaty, 1L (NCDWR); North Prong Lewis Fork, nr SR 1300, 36.21309, -81. 35806, 7 May 2010, V. Holland, M. Walters, 2L (NCDWR); Yadkin River, nr Abtco Rd., 36.19694, -81.02917, 7 June 1993, D. Penrose, 1L (NCDWR). Yadkin Co., North Deep Creek, NC 601, 36.16861, -80.68111, 12 April 1993, D. Lenat, 6L (NCDWR). South Carolina: Aiken Co., Tinker Creek, Savannah River Site, SRP B-1, [33.33769, -81.60466], 1 June 1977, Herlong, Prichard, 3F (NCDWR); same data, 40M, 7F (INHS, Insect Collection 875859); same data, 4M, 119F (INHS, Insect Collection 875917); same data, 136F (INHS, Insect Collection 875919); same data 1M, 62F (INHS, Insect Collection 875902); same data, 1M, 165F (INHS, Insect Collection 875845); same data, 1M, 100F (INHS, Insect Collection 875868); same location, 28 June 1977, Herlong, Prichard, 5F (NCDWR); Upper Three Runs, Savannah River Site Rd. C, [33.28518, -81.69533], 5 May 1984, B.C. Kondratieff, 29M, 8F (NCDWR); same location, 27 May 1984, B. Kondratieff, 1M, 27F (NCDWR); same location, 4 June 1984, B. Kondratieff, 14M, 26F (NCDWR); same location, 17 April 1985, B. Kondratieff, 2M, 1E (NCDWR); Upper Three Runs, Savannah River Site Rd. F, [33.23926, -81.74427], 4 May 1977, Herlong, Prichard, 4M, 1F (INHS, Insect Collection 875918); same location, 17 May 1977, Herlong, Prichard, 12M, 5F (INHS, Insect Collection 875916); same location, 1 June 1977, Herlong, Prichard, 3M, 159F (INHS, Insect Collection 875847); same data, 215F (INHS, Insect Collection 875861). Anderson Co., Rock Creek, N of S-4-670, [34.56723, -82.53546], 3–4 June 1980, J. Weaver, 1M (NCDWR). Barnwell Co., Meyers Branch, 600 m upstream from Savannah River Site Rd. A, [33.15049, -81.62530], 3 June 1983, B. Kondratieff, 5F (NCDWR); same location, 3 June 1983, B. Kondratieff, 4F (NCDWR); Steel Creek, Savannah River Site Rd. A, [33.14589, -81.62874], 3 June 1982, B. Kondratieff, 2M, 1F, 2E, (NCDWR); same data, 1M, 4F (NCDWR). Oconee Co., Chauga River, 4 mi S. of Mountain Rest, [34.83317, -83.15801], 10 June 1969, J. Morse, 1M, 2F (INHS, Insect Collection 875857). Pickens Co., Wildcat Creek, 9 km NW Clemson, [34.75589, -82.85776], 25 May 1980, J.S. Weaver III, 1F (INHS, Insect Collection 875870); South Saluda River, 4 mi N Pumpkintown, [35.06719, -82.61793], 27 June 1969, H. Douglas, Fox & Fox, 2M, 1F (INHS, Insect Collection 875858). Spartanburg Co., South Saluda River, 5 mi W Cleveland, [35.06759, - 82.61827], 2 June 1969, J. Morse, G. Townes, 2M (INHS, Insect Collection 875850); same location, 2 July 1969, H. Douglass, G. Townes, 1F (INHS, Inset Collection 875899). Tennessee: Blount Co., Abrams Creek, 100 m upstream Abrams Falls Trailhead, Great Smoky Mountains National Park, 35.59210, -83.85200, 8 June 2001, B.D. Heinold, 5F (INHS, Insect Collection 3383); same location, 6 July 2001, B.D. Heinold, R.E. DeWalt, S.L. Johnson, 1F (INHS, Insect Collection 3457). Virginia: Franklin Co., Big Chestnut Creek, Rte 718, [36.87630. W 79.86254], 21 May 2002, B.C. Kondratieff, R. F. Kirchner, 2M (VMNH); Grassy Fork, 2 mi S of Snow Creek, [36.81097, - 79.74516], 2 June 2000, R. L. Hoffman, 3M, 8F (VMNH); Pigg River, 0.3 km upstream of Rte 890 bridge SW Penhook, [36.94638, -79.64051, 26 June 2005, R. L. Hoffman, 1M, 7F (VMNH); Shooting Creek, Rte 860, 1.4 mi W jct. VA 40, [36.88060, -80.20147], 13 July 1991, R. L. Hoffman, 1F (VMNH). Halifax Co., no waterbody data, Berry Hill (Delorme 27? D5-6) at light in logged floodplain, [no coordinates], 8 June 1993, R. L. Hoffman, C. Hampton, 1F (VMNH); no waterbody data, swamp 2.5 mi E of Riceville, [no coordinates], 21 May 2000, (VMNH) survey, 1M (VMNH). Pittsylvania Co., Banister River, below Mt.Airy Mill, Rte 683, ca 1 mi SW Mt. Airy, [36.93180, -79.20300], 25 June 1989, R. L. Hoffman, 1M (VMNH); Crooked Run, Rte 841, 6 mi SW Callands, [36.74825, - 79.65121] 9 June 2000, R. L. Hoffman 3M, 5F (VMNH); Sandy River, Rte 845 bridge 4.5 mi N Brosville, [36.67935, -79.59389], 24 June 1993, R. L. Hoffman, 1F (VMNH); South Prong Sandy River, NW of Brosville, [36.66365, - 79.61798], 19 June 1990, R. L. Hoffman, 1F (VMNH); [Bawley Branch], jct. 939 & 614 3 mi SE of Sandy River, [36.71343, -79.61181], 2 June 1998, R. L. Hoffman, 1M, 2F (VMNH).

Isoperla lenati Szczytko & Kondratieff, 2015 (Figs. 1–9) Little Stripetail http//lsid.speciesfile.org/urn:lsid: Plecoptera.speciesfile.org:TaxonName:468919 Isoperla lenati Szczytko & Kondratieff 2015:137. Holotype male (USNM), Naked Creek, Richmond Co., North Carolina, U.S.A. Distribution. U.S.A. —FL, GA, NC, SC. (DeWalt et al. 2023; Fig. 15) Material examined. U.S.A. — North Carolina: Hoke Co., Flat Creek, Manchester Rd., 35.18244, -79.17695, 13 March 2019, emerged 21 March 2019, C. Verdone, S. Beaty, L. Housley, 2F, 2E (NCDWR); same location, 13 March 2019, emerged 25 March 2019, C. Verdone, S. Beaty, L. Housley, 1F, 1E (NCDWR); same location, 13 March 2019, emerged 26 March 2019, C. Verdone, S. Beaty, L. Housley, 1M (NCDWR); same location, 13 March 2019, emerged 29 March 2019, C. Verdone, S. Beaty, L. Housley, 1M, 2F, 3E (NCDWR); same location, 13 March 2019, emerged 11 April 2019, C. Verdone, S. Beaty, L. Housley, 2F, 2E (NCDWR); same location, 13 March 2019, C. Verdone, S. Beaty, L. Housley, 1M, 2F, 2E (NCDWR); same data, 1F, 1E (NCDWR); same data, C. Verdone, S. Beaty, L. Housley, 10L (NCDWR); Rockfish Creek, Plank Rd., 35.05861, -79.27907, 23 April 1990, NCDWR, 1L (NCDWR). Hoke / Scotland Co., Drowning Creek, Turnpike Rd., 34.97414, -79.37785, 19 May 2018, C. Verdone, 2F (NCDWR); Lumber River, NC 401, 34.90081, -79.34833, 28 March 2006, D. Lenat, 3L (NCDWR); same location, 25 March 2011, D. Lenat, 2L (NCDWR); same data, 2L (NCDWR); same location, 20 May 2018, C. Verdone, 1M (NCDWR); same location, 17 April 2019, C. Verdone, V. Holland, 2M, 5F (NCDWR); same data, 1M, 1F (NCDWR); same location, 9 May 2020, C. Verdone, V. Holland, 6M, 4F (NCDWR). Moore Co., Little River, Morrison Bridge Rd., 35.19233, -79.18408, 17 April 2019, C. Verdone, V. Holland, 4M, 7F (NCDWR); same data, 1L (NCDWR); unnamed tributary to Drowning Creek, SR 1140, 35.23711, -79.66511, 4 May 2006, E. Fleek, S. Beaty, 3L (NCDWR). Moore / Richmond Co., Drowning Creek, SR 1102, 35.08426, -79.58270, 28 March 2019, S. Beaty, C. Verdone, V. Holland, L. Housley, 1M, 1E (NCDWR); same data, 1M, 1E (NCDWR); same data, 2M (NCDWR); same location, 28 March 2019, emerged 14 April 2019, S. Beaty, C. Verdone, V. Holland, L. Housley, 1M, 1E (NCDWR); same location, 14 April 2019, C. Verdone, D. Fuller, S. Beaty, 2F (NCDWR); same data, 3M (NCDWR); same location, 2015, D. Lenat, 2L (NCDWR). Richmond Co., Big Branch, SR 1487, 35.02833, -79.63833, 2 May 2006, E. Fleek, S. Beaty, 1F, 1L (NCDWR); Naked Creek, SR 1003, 35.08197, -79.58909, 17 April 2019, C. Verdone, V. Holland, 2F (NCDWR); same location, 28 April 2019, S. Beaty, C. Verdone, 3F (NCDWR); same data, 1F (NCDWR).

Isoperla lenati Szczytko & Kondratieff, 2015 (Figs. 1–9) Little Stripetail http//lsid.speciesfile.org/urn:lsid: Plecoptera.speciesfile.org:TaxonName:468919 Isoperla lenati Szczytko & Kondratieff 2015:137. Holotype male (USNM), Naked Creek, Richmond Co., North Carolina, U.S.A. Distribution. U.S.A. —FL, GA, NC, SC. (DeWalt et al. 2023; Fig. 15) Material examined. U.S.A. — North Carolina: Hoke Co., Flat Creek, Manchester Rd., 35.18244, -79.17695, 13 March 2019, emerged 21 March 2019, C. Verdone, S. Beaty, L. Housley, 2F, 2E (NCDWR); same location, 13 March 2019, emerged 25 March 2019, C. Verdone, S. Beaty, L. Housley, 1F, 1E (NCDWR); same location, 13 March 2019, emerged 26 March 2019, C. Verdone, S. Beaty, L. Housley, 1M (NCDWR); same location, 13 March 2019, emerged 29 March 2019, C. Verdone, S. Beaty, L. Housley, 1M, 2F, 3E (NCDWR); same location, 13 March 2019, emerged 11 April 2019, C. Verdone, S. Beaty, L. Housley, 2F, 2E (NCDWR); same location, 13 March 2019, C. Verdone, S. Beaty, L. Housley, 1M, 2F, 2E (NCDWR); same data, 1F, 1E (NCDWR); same data, C. Verdone, S. Beaty, L. Housley, 10L (NCDWR); Rockfish Creek, Plank Rd., 35.05861, -79.27907, 23 April 1990, NCDWR, 1L (NCDWR). Hoke / Scotland Co., Drowning Creek, Turnpike Rd., 34.97414, -79.37785, 19 May 2018, C. Verdone, 2F (NCDWR); Lumber River, NC 401, 34.90081, -79.34833, 28 March 2006, D. Lenat, 3L (NCDWR); same location, 25 March 2011, D. Lenat, 2L (NCDWR); same data, 2L (NCDWR); same location, 20 May 2018, C. Verdone, 1M (NCDWR); same location, 17 April 2019, C. Verdone, V. Holland, 2M, 5F (NCDWR); same data, 1M, 1F (NCDWR); same location, 9 May 2020, C. Verdone, V. Holland, 6M, 4F (NCDWR). Moore Co., Little River, Morrison Bridge Rd., 35.19233, -79.18408, 17 April 2019, C. Verdone, V. Holland, 4M, 7F (NCDWR); same data, 1L (NCDWR); unnamed tributary to Drowning Creek, SR 1140, 35.23711, -79.66511, 4 May 2006, E. Fleek, S. Beaty, 3L (NCDWR). Moore / Richmond Co., Drowning Creek, SR 1102, 35.08426, -79.58270, 28 March 2019, S. Beaty, C. Verdone, V. Holland, L. Housley, 1M, 1E (NCDWR); same data, 1M, 1E (NCDWR); same data, 2M (NCDWR); same location, 28 March 2019, emerged 14 April 2019, S. Beaty, C. Verdone, V. Holland, L. Housley, 1M, 1E (NCDWR); same location, 14 April 2019, C. Verdone, D. Fuller, S. Beaty, 2F (NCDWR); same data, 3M (NCDWR); same location, 2015, D. Lenat, 2L (NCDWR). Richmond Co., Big Branch, SR 1487, 35.02833, -79.63833, 2 May 2006, E. Fleek, S. Beaty, 1F, 1L (NCDWR); Naked Creek, SR 1003, 35.08197, -79.58909, 17 April 2019, C. Verdone, V. Holland, 2F (NCDWR); same location, 28 April 2019, S. Beaty, C. Verdone, 3F (NCDWR); same data, 1F (NCDWR)., Published as part of Verdone, Chris J., Beaty, Steven R., Holland, Victor B. & Williams, Bronwyn W., 2023, Isoperla riverae, a new stonefly species from the southeast Nearctic, with notes on sympatric species including the larval description of Isoperla lenati Szczytko & Kondratieff, 2015 (Plecoptera: Perlodidae), pp. 437-470 in Zootaxa 5270 (3) on page 439, DOI: 10.11646/zootaxa.5270.3.3, http://zenodo.org/record/7860146, {"references":["Szczytko, S. W. & Kondratieff, B. C. (2015) A review of the eastern Nearctic Isoperlinae (Plecoptera: Perlodidae) with the description of twenty-two new species. Monographs of Illiesia, No. 1, 1 - 289. [http: // illiesia. speciesfile. org / papers / Monographiae-of-Illiesia. pdf]","DeWalt, R. E., Maehr, M. D., Hopkins, H., Neu-Becker, U. & Stueber, G. (2023) Plecoptera Species File Online. Version 5.0 / 5.0. Available from: http: // Plecoptera. SpeciesFile. org / (accessed 26 January 2023)"]}

Verdone, Chris J., Beaty, Steven R., Holland, Victor B., Williams, Bronwyn W. (2023): Isoperla riverae, a new stonefly species from the southeast Nearctic, with notes on sympatric species including the larval description of Isoperla lenati Szczytko & Kondratieff, 2015 (Plecoptera: Perlodidae). Zootaxa 5270 (3): 437-470, DOI: 10.11646/zootaxa.5270.3.3, URL: http://dx.doi.org/10.11646/zootaxa.5270.3.3

Isoperla davisi James, 1974 (Figs. 10–14) Alabama Stripetail http//lsid.speciesfile.org/urn:lsid: Plecoptera.speciesfile.org:TaxonName:468717 Isoperla davisi James 1974:996. Holotype male (INHS), Saugahatchee Creek, Tallapoosa Co., Alabama, U.S.A. Isoperla coushatta Szczytko & Stewart 1976:211. Holotype male (INHS), Saddler Creek, Anderson Co., Texas, U.S.A. Syn. Szczytko & Kondratieff, 2015:73. Isoperla coushatta: Szczytko & Stewart 1977:358. Isoperla coushatta: Poulton & Stewart 1991:47. Isoperla davisi: Szczytko & Kondratieff 2015:73. Distribution. USA: AL, AR, DE, FL, LA, MO, MS, NC, OK, SC, TX, VA. (DeWalt et al. 2023) Material examined. U.S.A. — North Carolina: Alamance Co., Haw River, NC 87, 36.18250, -79.50944, 2 May 1985, T. MacPherson, 4L (NCDWR). Bladen Co., Browns Creek, NC 87, 34.61339, -78.58489, 7 February 2008, S. Beaty, M. Walters, 6L (NCDWR). Cleveland Co., Lick Branch, SR 2227, 35.17694, -81.50111, 17 March 1986, D. Penrose, 15L (NCDWR). Edgecombe Co., Whiteoak Swamp, SR 1428, 36.00444, -77.62028, 3 May 1988, T. MacPherson, 1M, 13L (NCDWR); Whiteoak Swamp, NC 43, 36.00444, -77.62028, 5 February 2007, B. Crouch, B. Prusha, M. Walters, 9L (NCDWR). Gaston Co., Beaverdam Creek, SR 1609, 35.40444, -81.24528, 5 May 2006, B. Prusha, M. Walters, 9L (NCDWR). Guilford Co., Hickory Creek, SR 1132, 35.94027, -79.86888, 16 April 2003, K. Herring, B. Crouch, 10L (NCDWR). Iredell Co., Patterson Creek, SR 1890, 35.92417, -80.82417, 4 April 2006, E. Fleek, T. MacPherson, 14L (NCDWR). Moore Co., UT McLendons Creek, SR 1261, 35.34964, -79.47894, 28 March 2019, emerged 11 April 2019, S. Beaty, C. Verdone, V. Holland, L. Housley, 1M, 1E (NCDWR). Moore/ Richmond Co., Drowning Creek, SR 1102, 35.08426, -79.58270, 28 March 2019, emerged 8 April 2019, S. Beaty, C. Verdone, V. Holland, L. Housley, 1M, 1E (NCDWR); same location, 28 March 2019, S. Beaty, C. Verdone, V. Holland, L. Housley, 1M, 1E (NCDWR); same location, 17 April 2020, S. Beaty, V. Holland, 2M, 2F (NCDWR); Nash Co., Swift Creek, SR 1310, 36.11167, -77.92111, 24 April 2003, E. Fleek, T. MacPherson, 11L (NCDWR); Richmond Co., Naked Creek, SR 1003, 35.08197, -79.58909, 12 March 2019, C. Verdone, V. Holland, S. Beaty, 1L (NCDWR); same location, 28 March 2019, emerged 4 April 2019, S. Beaty, C. Verdone, V. Holland, L. Housley, 2M, 2E (NCDWR); same location, 28 March 2019, S. Beaty, C. Verdone, V. Holland, L. Housley, 5L (NCDWR); Stokes Co., Little Yadkin River, SR 1104, 36.29888, -80.41388, 6 May 1987, D. Penrose, 1L (NCDWR); Wake Co., unnamed tributary to Middle Creek, Optimist Farm Rd., 35.65831, -78.73863, 23 April 2019, C. Verdone, D. Fuller, 2M (NCDWR); Yadkin Co., North Deep Creek, SR 1510, 36.12583, -80.59222, 12 April 1993, D. Lenat, K. Herring, T. MacPherson, 11L (NCDWR); North Deep Creek, SR 1503, 36.17194, -80.67500, 12 April 1993, D. Lenat, K. Herring, T. MacPherson, 8L (NCDWR). Adult male, female, and ovum. Described in Szczytko & Kondratieff (2015). Mature larva (Figs. 1–4). Pre-emergent male larvae 4.9–5.5 mm (n = 6), female larvae 5.2–6.1 (n = 12). Body small and slender with contrasting body pattern (Fig. 1). Body usually with inconspicuous clear clothing hairs, some specimens with darker hairs. Dorsum of head (Fig. 2). Ground color pale yellow with mottled brown mask and with a pale medial area anterior to median ocellus, a pair of large anterolateral pale spots, a pair of mediolateral pale spots, a small enclosed pale ocellar spot, and pale ocular areas; pale median area sub-trapezoidal and slightly wider anteriorly with anterior edge usually somewhat straight; fully enclosed interocellar spot triangular to subtriangular and reaching anterior ocellus, removed from lateral ocelli; frontoclypeus with additional semi-quadrate anterolateral pale areas; brown mask composed of an irregular median transverse stripe, lateral arms of stripe directed posteriorly from pale median area with an abrupt anterolateral turn to antennal base, similar to a bent arm; mask with posterior extensions to lateral ocelli; brown bar on anterior frontoclypeus with darker brown tentorial spots or dashes at vertices; area between eyes and lateral ocelli pale, mediolateral tentorial callosities brown, barely visible; occiput with irregular brown areas posterior to epicranial suture and on either side of the epicranial stem, stem overlaid with a triangular pale spot; an oblique brown stripe from the post-occipital margin reaching post ocular margin, spicules scattered along oblique stripe; labrum pale yellow with median area marginally darker, thinly edged with brown. Labrum yellow-brown, slightly darker basomedially. Antennal scape and pedicel yellow to yellow-brown, flagellum yellow with apical segments slightly darker; flagellum with 28–31 antennomeres (n=11); each segment with an apical circlet of small spines. Pale to brown clothing hairs covering head. Maxilla (Figs. 5–6). Lacinia bidentate, receding evenly from base to subapical tooth; a long thin marginal seta between apical and subapical tooth present, and often a second shorter seta present at base of subapical tooth; 6–7 striated, stout, socketed marginal setae below subapical tooth, setae progressively thinner and shorter towards base; anterior marginal setae barely smaller than subapical tooth giving lacinia an overall comb-like appearance; 2–3 thin, unevenly-spaced setae just below last marginal seta on palm edge; a submarginal row of 3 closely set striated, stout, socketed setae below apical tooth, a fourth smaller seta in-line but slightly removed posteriorly; ventral face of palm with 3–4 setae, some bifurcate at tip, most set away from palm edge. Length of striated apical tooth of lacinia approximately 1/3 the palm length (0.38X) and 2/3 the basal palm width (0.64–0.72X). Subapical tooth about 2/3 (0.63–.68X) the length of the apical tooth. Galea about 0.9X lacinial palm length, with a sparse ventral row of thin setae and tipped with 1–3 apical long spines. Maxillary palp stout, about 1.5X (1.53–1.58X) length of lacinia; segments 1–4 successively longer, segment 5 subequal to segment 3; palp segments 1 and 2 with transverse row of spinous setae at apex; segment 3 with 1–2 spines on body of segment and 1–2 apical spines, segment 5 tipped with 1–2 sensillae. Mandibles (Figs. 7–8). Right mandible (Fig. 7) bicuspid, outer cusp with three teeth, ventral tooth largest, dorsal tooth smallest, tooth serrations indistinct or worn; ventral surface of mandible with an unorganized band of long spinous setae from base to outer ventral tooth, setae nearest ventral tooth thicker and with setules and serrations; inner cusp with three teeth, middle tooth longest and largest; a comb-like row of small sharply pointed acanthae at base of basal tooth, number and size of acanthae variable (Fig. 7 inset); molar ridge with a dense row of spine-like setae, setae longer and thinner towards base of mandible; dorsal surface of mandible also with band of unorganized serrated setae from inner cusp towards base of mandible, setae near cusp larger and with setules. Left mandible (Fig. 8) similar to right mandible except comb-like row of sharply pointed acanthae at base of inner cusp replaced by a dense brush of short spinous setae from basal tooth on inner cusp to molar ridge (Fig. 8 inset). Thorax (Figs. 1–2). Pronotum (Figs. 1–2) about twice as wide as long, extensive brown patterning consisting of two brown “O” shaped markings with pale centers, one on each disk, and connected by a narrow anterior transverse band; a brown stripe along the medial suture, widest posteriorly, joins to an anterior transverse brown bar forming a “ T ”; brown pronotal markings encompass many small dark rugosities; a narrow pale stripe on either side of the brown median stripe; pronotal flange edged in brown on anterior and posterior margins, narrowly interrupted medially, widely pale laterally; about 1/3 of anterior pronotal furrow pale; pronotum edged with closely set row of stiff setae, with longer bristles interspersed, the longest on lateral and posterior corners and approximately 1/8 the width of pronotum. Mesonotal and metanotal discs with irregular brown longitudinal markings (Fig. 1); wing pads yellow-brown with one (mesonotum) or two (metanotum) faint longitudinal brown bands, inner stripe of metanotum widest posteriorly; lateral margins of nota fringed with a short, closely set row of stiff bristles, with intervening long bristles truncate, mesonotum additionally with a cluster of up to 10 longer stiff bristles on the anterolateral corners. Thoracic nota with sparse light brown to pale clothing hairs. Lateral aspect of thorax pale yellow with notal trochantins brown to dark brown at apex; procoxal process conspicuous, dark brown. Thoracic sterna pale, without distinctive markings; basisterna with short clear setae; furcal pits conspicuous, Y-stem arms inconspicuous. Entire sternum with pale to light brown clothing hairs. Legs (Fig. 9). Pale yellow to yellow-brown overall. Femoral-tibial ratio 0.9 to 1.0 for each leg (n = 12). Posterior face of femora with a sparse and uneven medial row of stout golden spines; anterior face of femora with golden spines scattered along length, with a row of short spines along posterior edge of sclerite; dorsal edge of femora with a cluster of bristles in apical half, longest bristles about half the width of corresponding femur, many bristles truncate; femora with silky setae very sparse to absent (Fig. 9). Tibiae with two longitudinal dorsal rows of short spines and a sparse dorsal row of long silky setae along length, setae twice as long as width of tibia; tibiae also with two ventral rows of spines. Tarsi pale yellow-brown with a comb-like ventral row of stiff setae and a sparse dorsal row of silky setae. Two claws darker apically, moderately long and gradually curved. Abdomen (Figs. 1, 3–4). Terga yellow-brown with three dark brown longitudinal stripes, two lateral and one median, often bordered by obscure pale lines (Figs. 1, 3); anterior half of each segment with a transverse row of eight small, variably dark dots, obscure in some specimens; posterior edges of each tergal sclerite bearing a transverse row of golden spines interspersed with long truncate to slightly clavate bristles, longest bristles about ¾ length of respective segment; terga also with a few curved erect setae on body of sclerite. Abdominal sterna (Fig. 4) yellow-brown, darker than thoracic sterna; posterior edges of sterna bearing a transverse row of short golden spines, successively longer on distal segments; first and last dot of dorsal transverse row visible in ventral aspect on segments 9 and 10. Clothing hairs clear to light brown. Paraprocts triangular, pointed, 1.3–1.5X as long as wide. Cerci yellow-brown; each cercal segment with an apical circlet of spines, and bearing one long ventral spine about 2/3 length of respective segment and one dorsal spine about ½ length of segment; distal third of cerci with a sparse setal fringe, bearing 1–3 fine dorsal setae. Diagnosis. The larval habitus of I. lenati is defined by the following combination of characters: small body size (4.9–6.1 mm, n = 12); head pattern with a pale medial area anterior to median ocellus with a straight anterior margin, a pair of large anterolateral pale areas, a small enclosed pale ocellar spot, a pair of mediolateral pale spots that are confluent with the lateral ocelli and pale ocular areas; each pronotal disc with a brown “O” marking; femora with a sparse to absent fringe of silky setae and with long truncate bristles in apical half; three dark longitudinal abdominal stripes bordered by pale lines. Additionally, the overall habitus of this species appears quite bristly. Taxonomic notes. The larva of I. lenati is superficially most similar to I. davisi (Figs. 10–14), with which it is sometimes sympatric. Both taxa have striped abdomens (Figs. 3, 13) and the same configuration of markings on the head and pronotum (Figs. 2, 12). The diagnostic characters listed above differ from I. davisi in the following: pale medial area anterior to median ocellus is pointed anteriorly in I. davisi (Fig. 11–12), femora of I. davisi have long silky setae (Fig. 10), and the abdominal stripes have more pronounced dark submedial stripes (Fig. 13)., Published as part of Verdone, Chris J., Beaty, Steven R., Holland, Victor B. & Williams, Bronwyn W., 2023, Isoperla riverae, a new stonefly species from the southeast Nearctic, with notes on sympatric species including the larval description of Isoperla lenati Szczytko & Kondratieff, 2015 (Plecoptera: Perlodidae), pp. 437-470 in Zootaxa 5270 (3) on pages 439-446, DOI: 10.11646/zootaxa.5270.3.3, http://zenodo.org/record/7860146, {"references":["James, A. M. (1974) Four new species of stoneflies in North America (Plecoptera). Annals of the entomological society of America, 67, 964 - 966. https: // doi. org / 10.1093 / aesa / 67.6.964","Szczytko, S. W. & Stewart, K. W. (1976) Three new species of Nearctic Isoperla (Plecoptera). Great Basin Naturalist, 36, 211 - 220. [https: // scholarsarchive. byu. edu / cgi / viewcontent. cgi? article = 1869 & context = gbn] https: // doi. org / 10.5962 / bhl. part. 17201","Szczytko, S. W. & Kondratieff, B. C. (2015) A review of the eastern Nearctic Isoperlinae (Plecoptera: Perlodidae) with the description of twenty-two new species. Monographs of Illiesia, No. 1, 1 - 289. [http: // illiesia. speciesfile. org / papers / Monographiae-of-Illiesia. pdf]","Szczytko, S. W. & Stewart, K. W. (1977) The stoneflies (Plecoptera) of Texas. Transactions of the American Entomological Society, 103, 327 - 378. [https: // www. jstor. org / stable / 25078206]","Poulton, B. C. & Stewart, K. W. (1991) The stoneflies of the Ozark and Ouachita Mountains (Plecoptera). Memoirs of the American Entomological Society, 38, 1 - 116. [https: // www. biodiversitylibrary. org / page / 38911829 # page / 11 / mode / 1 up]","DeWalt, R. E., Maehr, M. D., Hopkins, H., Neu-Becker, U. & Stueber, G. (2023) Plecoptera Species File Online. Version 5.0 / 5.0. Available from: http: // Plecoptera. SpeciesFile. org / (accessed 26 January 2023)"]}

Isoperla riverae sp. n. Verdone, Beaty, Holland & Williams (Figs. 16–39) Isoperla dicala: Stark & Gaufin, 1978:401 Isoperla dicala: Szczytko & Kondratieff, 2015:88 (in part) Etymology. The new species is named for the senior author’s daughter. May she enjoy the splendor of the natural world and value all its inhabitants. The proposed common name is “River’s Stripetail”. Distribution. U.S.A. —AL, FL, GA, NC, MS, SC, TN, VA (Fig. 15) Material examined. Holotype M: U.S.A. — North Carolina: Moore / Richmond Co., Drowning Creek, SR 1102, 35.08426, -79.58270, 28 April 2019, emerged 6 May 2019, C. Verdone, S. Beaty (NMNH). Paratypes: Georgia: DeKalb Co., Snapfinger Creek, 5 mi SE of Panthersville, [33.66552, -84.21116], 4 June 1945, P.W. Fattig, 9M (INHS, Insect Collection 875867). Gwinnett Co., Ivy Creek, 5 mi NS Buford, [34.04788, -84.03133], 11 June 1945, P.W. Fattig, 2M (INHS, Insect Collection 875931). North Carolina: Caldwell Co., Johns River, Old Johns River Rd., [35.93359, -81.69053], 17 May 2004, B. Kondratieff, D. Lenat, R. F. Kirchner, R. Zuellig, 1M (NCDWR); same location, 20 May 2019, C. Verdone, S. Beaty, V. Holland, 1M (NCDWR). Henderson Co., French Broad River, Hwy 64, [35.32853, -82.57524], 23 May 1990, B. Kondratieff, R. F. Kirchner, 5M, 3F (NMNH). Moore Co., Drowning Creek, SR 1004, 35.06575, -79.54998, 28 April 2022, emerged 4 May 2022, C. Verdone, S. Beaty, V. Holland, 1M (NCDWR). Moore / Richmond Co., Drowning Creek, SR 1102, 35.08426, -79.5827, 19 May 2018, C. Verdone, 2M, 61F (NCDWR); same location, 28 April 2019, emerged 6 May 2019, C. Verdone, S. Beaty, 1M, 1E (NCDWR); same location, 28 April 2019, emerged 6 May 2019, C. Verdone, S. Beaty, 2M (NCDWR); same location, 28 April 2019, emerged 13 May 2019, C. Verdone, S. Beaty, 1M, 1E (NCDWR). same location, 28 April 2019, emerged 13 May 2019, C. Verdone, S. Beaty, 1F (NCDWR). Richmond Co., Naked Creek, SR 1003, 35.08197, -79.58909, 27 April 2012, emerged 30 April 2012, D. Lenat, 1F,1E (NCDWR); same location, 27 April 2012, emerged 5 May 2012, D. Lenat, 2F, 2E (NCDWR); same location, 28 April 2019, C. Verdone. S. Beaty, 1F, 1E, 3L (NCDWR); same location, 30 April 2019, C. Verdone, S. Beaty, 1F, 1E (NCDWR); same location, 9 May 2020, C. Verdone, V. Holland, 1M, 1F (NCDWR). Rockingham Co., Mayo River, SR 1358, 36.53483, -79.99086, 11 May 2018, C. Verdone, S. Beaty, V. Holland, 1M (NCDWR); same location, 15 May 2018, C. Verdone, S. Beaty, V. Holland, L. Housley, 2M (NCDWR); same location, 21 May 2019, V. Holland, 1M (NCDWR). South Carolina: Aiken Co., Upper Three Runs, Savannah River Site Rd. C, [33.28518, -81.69533], 5 May 1984, B. Kondratieff, 29M, 8F (NCDWR). Virginia: Franklin Co., Big Chestnut Creek, Rte 718 E, [36.87630, -79.86254], 21 May 2002, B. Kondratieff, R. F. Kirchner, 2M (NMNH). Additional material examined. U.S.A. — Alabama: Clarke Co., Fishers Creek, nr Whatley, [31.63982, - 87.71937], 8 May 1986, S. Harris, P. O’Neil, 44M, 11F (NCDWR). Cleburne Co., Shoal Creek, Shoal Creek Campground, [33.77071, -85.55572], 25 May 1984, S. Harris, P. Lago, 4M, 2F (NCDWR); South Fork Terrapin Creek, Hwy 55, [33.86017, -85.52440], 25 May 1984, S. Harris, P. Lago, 3M, 1F (NCDWR). Monroe Co., Little River, Hwy 59, [31.29888, -87.71048], 11 May 1982, S. Harris, 1M (NCDWR). Randolph Co., Cutnose Creek, Hwy 48, [33.42541, -85.37436], 20 May 1984, S. Harris, P. Lago, 1M, 2F (NCDWR). Tallapoosa Co., Josie Leg Creek, off Hwy 22, [32.98634, -85.85926], 19 June 1983, S. Harris, P. Lago, 1M (NCDWR). Florida: Escambia Co., Canoe Creek, above Bratt Rd., [30.95664, -87.34758], 24 April 2009, D. Ray, 1M (NCDWR). Walton Co., Alaqua Creek, Base Rd. 201, Eglin Air Force Base, [30.61667, -86.16444], 17 April 2006, R. W. Flowers, M.L. Pescador, A.K. Rasmussen, B.A. Richard, 10M (NCDWR). Georgia: Bartow Co., Clear Creek, 6 mi N Cartersville, [34.27560, -84.87250], 3 June 1939, P.W. Fattig, 1M (INHS, Insect collection 875885); Pettit Creek, 2 mi N Cartersville, [34.22127, -84.80251], 3 June 1939, P.W. Fattig, 2F (INHS, Insect Collection 875855). Fulton Co., Nancy Creek, 8 mi N Atlanta, [33.86331, -84.40475], 26 June 1945, P.W. Fattig, 3F (INHS, Insect Collection 875853). Hall Co., no waterbody data, Gainesville, [34.29778, -83.82417], 9 May 1944, T. H. Frison, H.H. Ross, 1F (INHS, Insect Collection 875862). Screven Co., Brier Creek, 7 mi NE Sylvania, [32.83172, -81.56612], D.C. Scott, 1M (INHS, Insect Collection 875895). Union Co., Wolf Creek, 3.5 mi N Neel [s] Gap, [34.78515, -83.90730], 30 May 1943, P.W. Fattig, 3M (INHS, Insect Collection 875891); same location, 8 June 1945, P.W. Fattig, 5M (INHS, Insect Collection 875875). Mississippi: Clarke Co., Moore Mill Creek, Clarkco State Park, [32.10818, -88.69334], 26 May 1990, Tanner, Parham, 1F (NCDWR). Lamar Co., Sandy Run, Okahola Rd., [31.20737, -89.38775], 13 May 1992, B. Stark, M. Hicks, 2F (NCDWR). Pike Co., Tangipahoa River, 3 mi Magnolia, [31.11275, -90.47943], 25 March 1977, B. Stark, 2M, 1F (NCDWR). Simpson Co., Mill Creek, Bushtowns Shiver Rd., [31.84672, - 89.98869], 12 May 1992, B. Stark, E. Stark, C. Bishop, 1M, 5F (NCDWR); Mill Creek, Hwy 472, [31.86365, - 89.99195], 3 May 1990, B. Stark, 2M, 5F (NCDWR); Strong River, 3 mi W of Pinola, Hwy 28, [31.88622, - 89.99349], 22 June 1988, S. Szczytko, 1M (NCDWR); Westville Creek, Jake Barnes Rd. blw. Hale Branch jct., [31.87234, -89.91891], 13 April 1991, B. Stark, 6M, 3F (NCDWR). Smith Co., Clear Creek, 3 mi SW Mize [31.84949, -89.59955], 24 April 1991, B. Stark, D. Tanner, 12F (NCDWR). North Carolina: Alleghany Co., Glade Creek, SR 1422, nr mouth, 36.50000, -81.03639, 20 June 2008, S. Beaty 15L (NCDWR). Ashe Co., Cranberry Creek, SR 1609, 36.4028, -81.29833, 13 May 1998, D. Lenat, 1L (NCDWR); Peak Creek, Off SR 1599, 350M upstream Ore Knob Branch, 36.42083, -81.31944, 15 April 1991, D. Penrose, 1L (NCDWR); Cranberry Creek, SR 1609, 36.44028, -81.29833, 13 May 1998. D. Lenat, 1L (NCDWR). Buncombe Co., South Hominy Cr, Davis Creek Rd., 35.47831, -82.75003, 2 June 2010, V. Holland, 1L (NCDWR); South Hominy Creek, Dale Pen Rd., 35.48817, -82.74259, 1 June 2010, V. Holland, 1L (NCDWR); [Swannanoa River], Swannanoa, 35.59789, - 82.39984], 19 June 1938, T. H. Frison, T. H. Frison Jr., 2M (INHS, Insect Collection 875897); same data, 2M (INHS, Insect Collection 875851). Burke Co., Black Fox Cr, SR 1917, 35.66417, -81.63278. 19 April 1989, T. McPherson, 15L (NCDWR); Henry Fork, NC 18, 35.67361, -81.53611, 20 April 1988, D. Lenat, 25L (NCDWR); Henry Fork, SR 1803, 35.68944, -81.45028, 1 May 2006, B. Crouch, 15L (NCDWR); Henry Fork SR 1854, 35.69556, -81.42722, 1 May 2006, B. Crouch, 16L (NCDWR); Henry Fork, SR 1916, 35.66167, -81.60361, 2 May 2006, B. Crouch, 10L (NCDWR); Henry Fork, SR 1922, 35.66167, -81.63694, 19 April 1988, T. McPherson, 15L (NCDWR); same location, 2 May 2006, T. Mormon, 11L (NCDWR); Ivy Creek, SR 1919, 35.64111, -81.66167, 19 April 1988, D. Lenat, 1L (NCDWR); Jacob Fork, SR 1924, 35.59056, -81.56722, 2 May 2006, B. Crouch, 6L (NCDWR); Little Fork, FSR 228, 35.91056, -81.81833, 19 May 2015, M. Walters, 1L (NCDWR); Rock Creek, SR 1915, 35.64167, -81.55750, 19 April 1988, D. Lenat, 2L (NCDWR); Steels Creek, at FR 228, [35.912264, -81.838327] 25 June 1988, S. Dunkle, 1M (NCDWR); Upper Creek, FSR 982 upstream of Timbered Br, 35.92000, -81.79306, 13 June 1994, D. Penrose, 1L (NCDWR). Caldwell Co., Abingdon Creek, SR 1315, 35.91344, -81.60104, 18 April 2011, T. Mormon, 1L (NCDWR); Yadkin River, off SR 1372 downstream of Jackson Camp Creek, 36.06895, -81.58553. 23 May 2011, D. Black, E. Fleek, S. Beaty, 2L (NCDWR). Catawba Co., Henry Fork, SR 1124, 35.68417, -81.40278. 1 May 2006, B. Crouch, 16L (NCDWR). Cleveland Co., Brushy Creek, SR 1323, 35.33972, -81.61580, 16 May 1995, N. Guthrie, 1L (NCDWR). Davidson Co., Abbott Creek, SR 1735, 36.00833, -80.0775, 10 May 2006, D. Black, S. Beaty, B. Prusha, 4L (NCDWR); Brushy Fork. SR 1810, 35.92444, -80.18028, 14 May 2003, T. Mormon, 9L (NCDWR). Franklin Co., Sandy Creek, US 401, 36.24806, -78.25833, 3 May 1988, T. McPherson, 3L (NCDWR). Gaston Co., Beaverdam Creek, SR 1609, 35.40444, -81.24528, 5 May 2006, B. Crouch, B. Prusha, M. Walters, 15L (NCDWR); Hoyle Creek, SR 1836, 35.33472, -81.13389, 4 May 2006, B. Crouch, 2L (NCDWR); Long Creek, SR 1405, 35.30194, -81.31333, 4 April 1994, N. Guthrie, 1L (NCDWR); same location, 18 April 1995, D. Penrose, 1L (NCDWR); Long Creek, NC 275, 35.30222, -81.22278, 5 April 1991, L. Eaton, 1L (NCDWR); same location, 1 April 1992, D. Lenat, 1L (NCDWR); same location, 19 April 1995, D. Penrose, 1L (NCDWR); Long Creek, NC 274, 35.30778, -81.30333, 4 April 1994, D. Penrose, 1L (NCDWR); same location, 18 April 1995, D. Penrose, 2L (NCDWR); Long Creek, SR 1448, 35.31222, -81.26472, 4 April 1994, K. Herring, 1L (NCDWR), Long Creek, SR 1446, 35.31639, -81.27194, 5 April 1994, D. Penrose, 2L, (NCDWR); UT Long Creek, SR 1446, 35.31750, - 81.27194, 5 April 1994, N. Guthrie, 1L (NCDWR). Graham Co., Little Snowbird Creek, off SR 1115, 35.28697, -83.87841, 4 June 2014, M. Walters, 1L (NCDWR). Henderson Co., Mills River, SR 1353, 35.38778, -82.54611, 2 May 2011, T. Mormon, 2L (NCDWR); same location, 23 April 2012, E. Fleek, 1L (NCDWR); Mills River, NC 191 /280, 35.39139, -82.56806, 2 June 2009, T. Mormon, E. Fleek, M. Walters, 4L (NCDWR); North Fork Mills River, SR 1343, 35.39425, -82.61503, 3 May 2011, T. Mormon, 1L (NCDWR); South Fork Mills River, SR 1340, 35.37569, -82.6144, 1 June 2009, T. Mormon, E. Fleek, M. Walters, 1L (NCDWR). Iredell Co., Olin Creek, SR 1891, 35.94472, -80.84444, 9 May 2006, D. Black, B. Prusha, S. Beaty, 3L (NCDWR); Patterson Creek, SR 1890, 35.92417, -80.82417, 4 April 2006, E. Fleek, 2L (NCDWR); Rocky Creek, SR 1854, 35.99139, -80.85944, 5 June 2003, D. Lenat, 4L (NCDWR); Third Creek, SR 2318, 35.73889, -80.84444, 21 June 1989, D. Lenat, 1L (NCDWR); Third Creek, SR 1521, 35.80917, -81.00222, 8 May 2006, S. Beaty, D. Black, 1L (NCDWR). Jackson Co., [Savannah Creek] Fort Tatham Campground, [US 441], [35.29354, -83.26746], 8 July 1981, B.C. Kondratieff, 1F (INHS, Insect Collection 875913). Lincoln Co., Howards Creek, SR 1200, 35.49833, -81.34056, 3 May 2006, B. Crouch, 2L (NCDWR); Killian Creek, SR 1511, 35.41583, -81.02889, 8 June 1994, D. Lenat, 2L (NCDWR); Leepers Creek, SR 1354, 35.49861, -81.14619, 8 May 2006, B. Prusha, 4L (NCDWR); Pott Creek, SR 1217, 35.55194, -81.31889, 3 May 2006, T. Mormon, 1L (NCDWR). Macon Co., Burningtown Creek, Tellico Rd, 35.28277, -83.48240, 6 May 2008, T. Mormon, 1L (NCDWR); Caler Fork, off Ruby Mine Rd, 35.26860, -83.38656, 7 May 2008, R. Thorpe, 1L (NCDWR); Coon Creek, US 23/441, 35.23033, -83.34921, 9 May 2008, T. Mormon, 1L (NCDWR); Cowee Creek, Leatherman Gap Rd, 35.27046, -83.39047, 8 May 2008, R. Thorpe, 3L (NCDWR); Cowee Creek, NC 28, 35.2625, -83.40944, 23 May 2007, D. Black, 1L (NCDWR); Downes Branch, SR 1392, 35.21263, -83.49833, 28 April 2011, V. Holland, 1L (NCDWR); Iotla Branch, SR 1387, 35.22425, -83.42923, 7 May 2008, R. Thorpe, 3L (NCDWR); Iotla Creek, SR 1385, 35.21887, -83.42293, 7 May 2008, C. Tyndall, 2L (NCDWR); Iotla Creek, off SR 1378, 35.23473, -83.39824, 24 May 2007, D. Black, 2L (NCDWR); same location, 7 May 2008, T. Mormon, 1L (NCDWR); Mica City Creek, SR 1346, 35.2696, -83.37045, 25 April 2011, V. Holland, 3L (NCDWR); North Fork Coweeta Creek, SR 1114, 35.06525, -83.40253, 17 May 2011, V. Holland, 2L (NCDWR); Rabbit Creek, off SR 1504, 35.20690, -83.34836, 8 May 2008, R. Thorpe, 12L (NCDWR); Rabbit Creek, SR 1504, 35.20837, -83.35209, 8 May 2008, T. Mormon, 3L (NCDWR); Younce Creek, Younce Creek Rd., 35.23775, -83.47404, 26 April 2011, V. Holland, E. Fleek, S. Beaty, 1L (NCDWR). McDowell Co., Catawba River, SR 1273, 35.61944, -82.18833, 18 April 1985, NCDWR, 1L (NCDWR); Catawba River, SR 1234, 35.63694, -82.14389, 18 April 1985, NCDWR, 1L (NCDWR); North Muddy Creek, SR 1760, 35.67528, -81.90639, 17 April 1985, NCDWR, 16L (NCDWR); Youngs Fork, SR 1819, 35.65389, -81.96306, 17 April 1985, NCDWR, 1L (NCDWR). Nash Co., Sandy Creek, SR 1405, 36.12694, -78.02472, 21 April 2003, E. Fleek, 3L (NCDWR); Swift Creek, SR 1310, 36.11167, -77.92111, 24 April 2003, E. Fleek, 2L (NCDWR). Polk Co., Rotten Creek, off Jeep Trail 35.34709, -82.21117, 16 April 2014, V. Holland, 1L (NCDWR); Whiteoak Creek, SR 1 352, 35.26055, -82.00388, 15 May 1995, N. Guthrie, L. Eaton, K. Herring, 1L (NCDWR). Richmond Co., Naked Creek, SR 1003, 35.08194, -79.59028, 6 April 1990, L. Eaton, 2L (NCDWR); Naked Creek, SR 1003, 35.08197, -79.58909, 18 April 2013, D. Lenat, 1L (NCDWR); Rocky Ford Branch, SR 1424, 35.12417, -79.66250, 9 May 1990, D. Lenat, 8L (NCDWR). Rockingham Co., Falls Creek, Mayo River State Park, 36.539178, -79.988084, 1 May 2012, D. Lenat, 1L (NCDWR); Rock House Creek, SR 2127, 36.42083, -79.79055, 12 April 2001, L. Eaton, 1L (NCDWR). Rutherford Co., Second Broad River, above Union Mills, 35.47917, -81.94417, 19 May 1999, K. Herring, 4L (NCDWR); Second Broad River, below Union Mills, 35.47667, -81.94250, 19 May 1999, K. Herring, 1L (NCDWR) Second Broad River, SR 1504, 35.48401, - 81.94921, 18 May 2010, D. Black, S. Beaty, E. Fleek, 1L (NCDWR). Stokes Co., Brushy Fork, SR 1998, 36.32306, -80.30278, 18 May 2004, N. Flint, 1L (NCDWR); East Prong Little Yadkin River, SR 1224, 36.33126, -80.38586, 17 May 1994, N. Guthrie, 4L (NCDWR); Little Yadkin River, SR 1104, 36.29889, -80.41389, 18 May 1994, D. Penrose, 1L (NCDWR); same location 31 May 1989, D. Penrose, 1L (NCDWR); Little Yadkin River, SR 1104, 36.29899, -80.41388, 6 May 1987, D. Penrose, 2L (NCDWR); same location, 31 May 1989, D. Penrose, 1L (NCDWR). Surry Co., Endicott Creek, Raven Knob Rd., 36.47274, -80.85152, 5 May 2010, V. Holland, M. Walters, 2L (NCDWR); Fisher River, nr SR 1346, 36.41806, -80.71752, 4 May 2010, V. Holland, M. Walters, 4L (NCDWR). Transylvania Co., Catheys Creek, SR 1338, 35.20750, -82.78056, 21 May 1987, D. Penrose, 1L (NCDWR). Wilkes Co., Elk Creek, SR 1175, 36.10639, -81.43808, 7 June 2006, M. Walters, 1L (NCDWR); Left Prong Stony Fork, off SR 1155, 36.15065, -81.45392, 20 April 2012, S. Beaty, 1L (NCDWR); North Prong Lewis Fork, nr SR 1300, 36.21309, -81. 35806, 7 May 2010, V. Holland, M. Walters, 2L (NCDWR); Yadkin River, nr Abtco Rd., 36.19694, -81.02917, 7 June 1993, D. Penrose, 1L (NCDWR). Yadkin Co., North Deep Creek, NC 601, 36.16861, -80.68111, 12 April 1993, D. Lenat, 6L (NCDWR). South Carolina: Aiken Co., Tinker Creek, Savannah River Site, SRP B-1, [33.33769, -81.60466], 1 June 1977, Herlong, Prichard, 3F (NCDWR); same data, 40M, 7F (INHS, Insect Collection 875859); same data, 4M, 119F (INHS, Insect Collection 875917); same data, 136F (INHS, Insect Collection 875919); same data 1M, 62F (INHS, Insect Collection 875902); same data, 1M, 165F (INHS, Insect Collection 875845); same data, 1M, 100F (INHS, Insect Collection 875868); same location, 28 June 1977, Herlong, Prichard, 5F (NCDWR); Upper Three Runs, Savannah River Site Rd. C, [33.28518, -81.69533], 5 May 1984, B.C. Kondratieff, 29M, 8F (NCDWR); same location, 27 May 1984, B. Kondratieff, 1M, 27F (NCDWR); same location, 4 June 1984, B. Kondratieff, 14M, 26F (NCDWR); same location, 17 April 1985, B. Kondratieff, 2M, 1E (NCDWR); Upper Three Runs, Savannah River Site Rd. F, [33.23926, -81.74427], 4 May 1977, Herlong, Prichard, 4M, 1F (INHS, Insect Collection 875918); same location, 17 May 1977, Herlong, Prichard, 12M, 5F (INHS, Insect Collection 875916); same location, 1 June 1977, Herlong, Prichard, 3M, 159F (INHS, Insect Collection 875847); same data, 215F (INHS, Insect Collection 875861). Anderson Co., Rock Creek, N of S-4-670, [34.56723, -82.53546], 3–4 June 1980, J. Weaver, 1M (NCDWR). Barnwell Co., Meyers Branch, 600 m upstream from Savannah River Site Rd. A, [33.15049, -81.62530], 3 June 1983, B. Kondratieff, 5F (NCDWR); same location, 3 June 1983, B. Kondratieff, 4F (NCDWR); Steel Creek, Savannah River Site Rd. A, [33.14589, -81.62874], 3 June 1982, B. Kondratieff, 2M, 1F, 2E, (NCDWR); same data, 1M, 4F (NCDWR). Oconee Co., Chauga River, 4 mi S. of Mountain Rest, [34.83317, -83.15801], 10 June 1969, J. Morse, 1M, 2F (INHS, Insect Collection 875857). Pickens Co., Wildcat Creek, 9 km NW Clemson, [34.75589, -82.85776], 25 May 1980, J.S. Weaver III, 1F (INHS, Insect Collection 875870); South Saluda River, 4 mi N Pumpkintown, [35.06719, -82.61793], 27 June 1969, H. Douglas, Fox & Fox, 2M, 1F (INHS, Insect Collection 875858). Spartanburg Co., South Saluda River, 5 mi W Cleveland, [35.06759, - 82.61827], 2 June 1969, J. Morse, G. Townes, 2M (INHS, Insect Collection 875850); same location, 2 July 1969, H. Douglass, G. Townes, 1F (INHS, Inset Collection 875899). Tennessee: Blount Co., Abrams Creek, 100 m upstream Abrams Falls Trailhead, Great Smoky Mountains National Park, 35.59210, -83.85200, 8 June 2001, B.D. Heinold, 5F (INHS, Insect Collection 3383); same location, 6 July 2001, B.D. Heinold, R.E. DeWalt, S.L. Johnson, 1F (INHS, Insect Collection 3457). Virginia: Franklin Co., Big Chestnut Creek, Rte 718, [36.87630. W 79.86254], 21 May 2002, B.C. Kondratieff, R. F. Kirchner, 2M (VMNH); Grassy Fork, 2 mi S of Snow Creek, [36.81097, - 79.74516], 2 June 2000, R. L. Hoffman, 3M, 8F (VMNH); Pigg River, 0.3 km upstream of Rte 890 bridge SW Penhook, [36.94638, -79.64051, 26 June 2005, R. L. Hoffman, 1M, 7F (VMNH); Shooting Creek, Rte 860, 1.4 mi W jct. VA 40, [36.88060, -80.20147], 13 July 1991, R. L. Hoffman, 1F (VMNH). Halifax Co., no waterbody data, Berry Hill (Delorme 27? D5-6) at light in logged floodplain, [no coordinates], 8 June 1993, R. L. Hoffman, C. Hampton, 1F (VMNH); no waterbody data, swamp 2.5 mi E of Riceville, [no coordinates], 21 May 2000, (VMNH) survey, 1M (VMNH). Pittsylvania Co., Banister River, below Mt.Airy Mill, Rte 683, ca 1 mi SW Mt. Airy, [36.93180, -79.20300], 25 June 1989, R. L. Hoffman, 1M (VMNH); Crooked Run, Rte 841, 6 mi SW Callands, [36.74825, - 79.65121] 9 June 2000, R. L. Hoffman 3M, 5F (VMNH); Sandy River, Rte 845 bridge 4.5 mi N Brosville, [36.67935, -79.59389], 24 June 1993, R. L. Hoffman, 1F (VMNH); South Prong Sandy River, NW of Brosville, [36.66365, - 79.61798], 19 June 1990, R. L. Hoffman, 1F (VMNH); [Bawley Branch], jct. 939 & 614 3 mi SE of Sandy River, [36.71343, -79.61181], 2 June 1998, R. L. Hoffman, 1M, 2F (VMNH)., Published as part of Verdone, Chris J., Beaty, Steven R., Holland, Victor B. & Williams, Bronwyn W., 2023, Isoperla riverae, a new stonefly species from the southeast Nearctic, with notes on sympatric species including the larval description of Isoperla lenati Szczytko & Kondratieff, 2015 (Plecoptera: Perlodidae), pp. 437-470 in Zootaxa 5270 (3) on pages 446-447, DOI: 10.11646/zootaxa.5270.3.3, http://zenodo.org/record/7860146, {"references":["Stark, B. P. & Gaufin, A. R. (1978) The stoneflies (Plecoptera) of Florida. Transactions of the American Entomological Society, 104, 391 - 433. [https: // www. jstor. org / stable / 25078229]","Szczytko, S. W. & Kondratieff, B. C. (2015) A review of the eastern Nearctic Isoperlinae (Plecoptera: Perlodidae) with the description of twenty-two new species. Monographs of Illiesia, No. 1, 1 - 289. [http: // illiesia. speciesfile. org / papers / Monographiae-of-Illiesia. pdf]"]}

Herhold, Hollister W, Davis, Steven R, Degrey, Samuel P, Grimaldi, David A (2023): COMPARATIVE ANATOMY OF THE INSECT TRACHEAL SYSTEM PART 1: INTRODUCTION, APTERYGOTES, PALEOPTERA, POLYNEOPTERA. Bulletin of the American Museum of Natural History 459 (1): 1-184, DOI: 10.5531/sd.sp.55, URL: http://dx.doi.org/10.5531/sd.sp.55

Key for the males of the Perlodini genera Two genera included in the PSF (DeWalt et al. 2022) as belonging to Perlodini, not treated herein: Hedinia Navás, 1936 is known only from the female holotype, while Protarcys Klapálek, 1912 is under redescription and assessment of its affinity. Recent studies proved that Rauserodes Zwick, 1999 is a junior synonym of Perlodinella Klapálek, 1912 (Huo et al. 2022a), and Sinoperlodes Chen, 2020 is a junior synonym of Filchneria Klapálek, 1908 (Huo et al. 2022b). 1 Tergum X not divided into hemiterga (e.g., figs. 3A, 7A in Huo et al. 2022a)...................................... 2 - Tergum X divided into hemiterga (e.g., Fig. 7).............................................................. 7 2 Paraproct modified into caudally directed, hook-like lobe; tergum X with lightly sclerotized medial field (figs. 132, 135 in Teslenko & Zhiltzova 2009)................................................... Diura Billberg, 1820 (Holarctic) - Paraproct not modified into hook-like lobe; tergum X entirely sclerotized or with lightly sclerotized field of variable extent.................................................................................................... 3 3 Eversible epiproct present (fig. 14E in Huo et al. 2022a)......................... Perlodinella Klapálek, 1912 (China) - Epiproct is lacking.................................................................................... 4 4 Eversible paraproct lobe with distinct sclerotized process (e.g., fig. 12 in Teslenko & Palatov 2020).................... 5 - Eversible paraproct lobe membranous, or only with slight sclerotization (e.g., fig. 9 in Huo et al. 2022b)................ 6 5 Paraproct with truncated knob (fig. 9 in Teslenko & Palatov 2020)............ Zhiltzovaia Özdikmen, 2008 (Central Asia) - Paraproct without knob (figs. 5, 8, 25 in Teslenko 2015)............................................................................................ Megaperlodes Yokoyama, Isobe & Yamamoto, 1990 (Pacific East Palaearctic) 6 At least one of terga 8–10 with sensilla basiconica patches (figs. 3B, 9A in Huo et al. 2022b).............................................................................. Filchneria Klapálek, 1908 (Caucasus and East Palaearctic) - Terga lack sensilla basiconica (figs. 129B–C in Kis 1974)......................... Perlodes Banks, 1903 (Palaearctic) 7 Furcal pit connecting anteriorly to arms of mesosternal ridge (Fig. 1).......................... Habaek gen. n. (Korea) - Furcal pit connecting posteriorly to arms of mesosternal ridge, or the connection is obscure (figs. 36A–D in Ricker 1952).. 8 8 Epiproct tip with arrow-like apex (e.g. figs. 8.33, 8.49 in Kondratieff 2004)....................................... 9 - Epiproct tip different................................................................................. 11 9 Furcasternum with longitudinal medial ridge (fig. 36D in Ricker 1952)............ Isogenoides Klapálek, 1912 (Nearctic) - Furcasternum lacks medial ridge........................................................................ 10 10 Submental gill distinctive (compare Fig. 4)............................... Hydroperla Frison, 1935 (Eastern Nearctic) - Submental gill lacking..................................................... Dictyogenus Klapálek, 1904 (Alps) 11 Epiproct tip modified into a long, asymmetrical curved process (figs. 55–56 in Teslenko & Zhiltzova 2009).................................. Levanidovia Teslenko & Zhiltzova, 1989 (in: Zhiltzova & Teslenko 1989) (Pacific East Palaearctic) - Epiproct tip symmetrical.............................................................................. 12 12 Hemitergum with swollen apex, armed with sensilla basiconica and/or dense, long setae (e.g., figs. 8.9–10 in Kondratieff 2004)............................................................................................. 13 - Hemitergum with widely rounded but not swollen apex, sensilla basiconica is usually lacking....................... 17 13 Lateral stylet of epiproct absent (e.g., figs 8.10, 8.13 in Kondratieff 2004)....................................... 14 - Lateral stylet of epiproct present (e.g., fig. 3C in Zwick 1971)................................................ 15 14 Submental gill distinctive; epiproct tip pointed (figs. 8.10, 8.13 in Kondratieff 2004).................................................................................................. Helopicus Ricker, 1952 (Eastern Nearctic) - Submental gill is lacking; epiproct tip swollen (figs. 1–3 in Stark et al. 1986).......................................................................... Guadalgenus Stark & González del Tánago, 1986 (in: Stark et al. 1986) (Iberia) 15 Epiproct tip with distinctive, hook-like apex (fig. 4 in Kondratieff et al. 2007)..................................................................................................... Chernokrilus Ricker, 1952 (Western Nearctic) - Epiproct tip blunt or with short, acute tip................................................................. 16 16 Hemiterga widely separated in the medial portion of the segment; epiproct tip acute (figs. 64, 67–68 in Teslenko & Zhiltzova 2009)................................................................. Isogenus Newman, 1833 (Palaearctic) - Hemiterga narrowly separated in the medial portion of the segment; epiproct tip blunt (figs. 3A–C in Zwick 1971)............................................................................ Besdolus Ricker, 1952 (West Palaearctic) 17 Lateral stylet of epiproct absent (e.g., figs. 8.68, 8.72–73 in Kondratieff 2004)................................... 18 - Lateral stylet of epiproct present (e.g., figs. 8.58, 8.61 in Kondratieff 2004)...................................... 19 18 Epiproct tip narrow and erect (figs. 8.69, 8.73 in Kondratieff 2004)................ Yugus Ricker, 1952 (Eastern Nearctic) - Epiproct tip membranous and coiled up (fig. 17F in Inada 1996)...............unnamed genus sensu Inada (1996) (Japan) 19 Lateral stylet of epiproct hooked at apex; epiproct tip with reddish setae (figs. 8.58, 8.61 in Kondratieff 2004).............................................................................. Malirekus Ricker, 1952 (Eastern Nearctic) - Lateral stylet of epiproct not hooked; epiproct tip without reddish setae......................................... 20 20 Submental gill absent; lateral stylet truncate (figs. 8.64–65 in Kondratieff 2004)............................................................................................. Oconoperla Stark & Stewart, 1982 (Eastern Nearctic) - Submental gill long; lateral stylet acute ……………………………………………………………..21 21 Head entirely black; epiproct tip with longitudinal dorsal sclerite strip not reaching apex (figs. 9A–B, G in Inada 1996)................................................................................. Tadamus Ricker, 1952 (Japan) - Head with yellow pattern; epiproct tip with longitudinal dorsal sclerite strip reaching apex (figs. 1, 4–6 in Bottorf et al. 1989).......................................................... Susulus Bottorf, Stewart & Knight, 1989 (California), Published as part of Murányi, Dávid & Hwang, Jeong Mi, 2023, A new genus and species of Perlodinae (Plecoptera: Perlodidae) from Korea, pp. 138-150 in Zootaxa 5249 (1) on pages 146-147, DOI: 10.11646/zootaxa.5249.1.8, http://zenodo.org/record/7685440, {"references":["DeWalt, R. E., Maehr, M. D., Hopkins, H., Neu-Becker, U. & Stueber, G. (2022) Plecoptera Species File Online. Version 5.0 / 5.0. Available from: http: // plecoptera. speciesfile. org (accessed 5 January 2022)","Navas, L. (1936) Schwedisch-chinesische wissenschaftliche expedition nach den nord-westlichen Provinzen Chinas. Plecoptera. Arkiv f ˆ r Zoologi, Uppsala, 27 (A 15), 1 - 11.","Klapalek, F. (1912) Plecopteres I. Fam. Perlodidae. Collections Zoologiques du Baron Edm. de Selys Longchamps, 4 (1), 1 - 66.","Zwick, P. (1999) Notes on Plecoptera (22) Rauserodes nom. n., replacement name for Rauserella Zwick (Plecoptera: Perlodidae). Aquatic Insects, 21 (3), 168. https: // doi. org / 10.1076 / aqin. 21.3.168.4518","Huo, Q. B., Du, Y. Z., Zwick, P. & Muranyi, D. (2022 a) Notes on Perlodinella Klapalek, 1912 (Plecoptera: Perlodidae) with a new species and a new synonym. Zootaxa, 5162 (4), 378 - 396. https: // doi. org / 10.11646 / zootaxa. 5162.4.3","Chen, Z. T. (2020) Sinoperlodes, a new genus of subfamily Perlodinae (Plecoptera, Perlodidae) from coastal southeastern China. Zootaxa, 4779 (4), 584 - 594. https: // doi. org / 10.11646 / zootaxa. 4779.4.9","Klapalek, F. (1908) Plecoptera. In: Filchner, W. (Ed.), Wissenschaftliche Ergebnisse der Expedition Filchner nach China und Tibet 1903 - 1905. 10 (1). 1. Abschnitt: Zoologische Sammlungen. 2. Abschitt: Botanische Sammlungen. Ernst Siegfried Mittler un Sohn, Berlin, pp. 59 - 64, pl. IV.","Huo, Q. B., Zhu, B. Q., Rehman, A., Muranyi, D., Du, Y. Z. & Wu, J. (2022 b) New synonym and new species record of Filchneria (Plecoptera: Perlodidae) from China with morphological, phylogenetic and biogeographic study on this genus. Insects, 13 (11), 1044, 1 - 19. https: // doi. org / 10.3390 / insects 13111044","Teslenko, V. A. & Zhiltzova, L. A. (2009) Key to the stoneflies (Insecta, Plecoptera) of Russia and adjacent countries. Imagines and nymphs. Dalnauka, Vladivostok, 382 pp. [in Russian]","Billberg, G. J. (1820) Enumeratio Insectorum in Museo Billberg. Typis Gadelianis, Holmiae, 120 pp. https: // doi. org / 10.5962 / bhl. title. 49763","Teslenko, V. A. & Palatov, D. M. (2020) Redescription of the remarkable Zhiltzovaia amabilis (Jewett, 1958) comb. nov. (Plecoptera, Perlodidae) based on types and new material from Himalaya and Pamir Mountain systems. Zootaxa, 4767 (2), 295 - 306. https: // doi. org / 10.11646 / zootaxa. 4767.2.5","Ozdikmen, H. (2008) A new name for the preoccupied stonefly genus Aubertiana Zhiltzova, 1994 (Plecoptera). Munis Entomology & Zoology, 3 (2), 761 - 762.","Teslenko, V. A. (2015) A new species of Megaperlodes Yokoyama et al. 1990 (Plecoptera, Perlodidae) from the South of the Russian Far East. Zootaxa, 3904 (4), 553 - 562. https: // doi. org / 10.11646 / zootaxa. 3904.4.4","Yokoyama, N., Isobe, Y. & Yamamoto, S. (1990) Distribution of Megaperlodes niger gen. et sp. nov. (Perlodidae) in Yamagata Prefecture. In: Abstracts of Papers from the 55 th Annual Meeting of the Limnological Society of Japan, Yamagata, Japan, 1990, pp. 29 - 30. [in Japanese]","Kis, B. (1974) Plecoptera. Fauna Republicii Socialiste Romania, 8 (7), 1 - 273.","Banks, N. (1903) New name for Dictyopteryx Pictet. Entomological News, 14 (7), 241.","Ricker, W. E. (1952) Systematic studies in Plecoptera. Indiana University Publications Science Series, 18, 1 - 200.","Kondratieff, B. C. (2004) Perlodidae - Perlodinae (The Springflies). In: Stark, B. P. & Armitage, B. J. (Eds.), The stoneflies (Plecoptera) of Eastern North America. Vol. II. Chloroperlidae, Perlidae, and Perlodidae (Perlodinae). Bulletin of the Ohio Biological Survey, New Series, 14 (4), pp. 149 - 180.","Frison, T. H. (1935) The stoneflies, or Plecoptera, of Illinois. Illinois Natural History Survey Bulletin, 20 (4), 281 - 471. https: // doi. org / 10.21900 / j. inhs. v 20.269","Klapalek, F. (1904) Ueber die Europaeischen arten der Fam. Dictyopterygidae. Bulletin international de l'Academie des Sciences de Boheme, 13 (17), 1 - 10.","Zhiltzova, L. A. & Teslenko, V. A. (1989) Opisanie Levanidovia mirabilis gen. et sp. n. i opredelitelnaya tablica lichinok rodov Perlodinae (Plecoptera, Perlodidae) Dalnego Vostoka. In: Levanidova, I. M. & Makarchenko, E. A. (Eds.), Sistematika i ekologiya rechnyh organizmov. DVO an SSSR, Vladivostok, pp. 5 - 14.","Zwick, P. (1971) Die Plecopteren Pictets und Burmeisters, mit Angaben uber weitere Arten (Insecta). Revue Suisse de Zoologie, 78 (4), 1123 - 1194. https: // doi. org / 10.5962 / bhl. part. 97087","Stark, B. P., Gonzalez del Tanago, M. & Szczytko, S. W. (1986) Systematic studies on Western Palaearctic Perlodini (Plecoptera: Perlodidae). Aquatic Insects, 8 (2), 91 - 98. https: // doi. org / 10.1080 / 01650428609361237","Kondratieff, B. C., Baumann, R. W. & Lee, J. J. (2007) The genus Chernokrilus Ricker (Plecoptera: Perlodidae). Illiesia, 3 (16), 163 - 170.","Newman, E. (1833) Entomological notes. The Entomological Magazine, London, 1 (47), 413 - 416.","Inada, K. (1996) Illustrated stonefly adults (Insecta, Plecoptera) of Hyogo Prefecture, Honshu, Japan, Part 1, Peltoperlidae and Perlodidae. Biology of Inland Waters, 11, 45 - 74. [in Japanese]","Stark, B. P. & Stewart, K. W. (1982) Oconoperla, a new genus of North American Perlodinae (Plecoptera: Perlodidae). Proceedings of the Entomological Society of Washington, 84 (4), 746 - 752. https: // doi. org / 10.1093 / aesa / 75.1.84"]}

Habaek gen. n. Diagnosis: Medium sized, dark brown Perlodinae with contrasting pale pattern on wings and rather long appendages; vestigial submental and thoracal gills present, furcal pit anteriorly connected to mesosternal ridge. Wings with enlarged costal field in both sexes, and unbranched Radial vein in the female. Male: sterna lack vesicle, terga lack sensilla basiconica, tergum X widely divided into triangular hemiterga; epiproct with large basal anchor and lateral arm, large paragenital plate and ventral projection, small lateral stylet and densely setose cowl, large and complex epiproct tip that is not connected with wide lever arm. Aedeagus displays a distinct pattern of golden scales. Female: subgenital plate reduced. Type species: Habaek koreanus sp. n., by monotypy. Affinities: The present tribal concept of the subfamily Perlodinae recognizes three tribes, based mainly on larval and egg characters and a few distinct characters of the male terminalia (Stark & Szczytko 1984, 1988). The lack of finger-like male hemiterga preclude the new genus from the tribe Arcynopterygini, while the lack of male sternal vesicle preclude it from Diploperlini. Consequently, we classify it in the Perlodini. However, discovery of the larva and matured egg may require modification of this classification. The spherical shape of immature eggs found in the paratype females already suppose classification in Arcynopterygini, since, with few exceptions, Perlodini species have eggs triangular in cross section, but it cannot be excluded that eggs become triangular when fully matured. The anteriorly connected furcal pit and mesosternal ridge is unique among Perlodini, while usual in Arcynopterigini (Arcynopteryx, Frisonia Ricker, 1943, Megarcys, Neofilchneria Zwick, 1973, Pseudomegarcys Kohno, 1946, Skwala Ricker, 1952) and rare in Diploperlini (Osobenus Ricker, 1952 and Stavsolus). Probably more important, and supportive of the new genus being classified in Perlodini, is the presence of fully divided but widely rounded hemiterga that lacks sensilla basiconica. This type of hemiterga occur only in the Perlodini: Tadamus Ricker, 1952 and an unnamed genus sensu Inada (1996) and Shimizu et al. (2005) from Japan, Malirekus Ricker, 1952, Oconoperla Stark & Stewart, 1982 and Yugus Ricker, 1952 from the eastern Nearctic, and Susulus Bottorf, Stewart & Knight, 1989 from California. The arrow-like apex of the epiproct tip is another character shared in only a few Perlodini genera (Dictyogenus Klapálek, 1904, Hydroperla Frison, 1935 and Isogenoides Klapálek, 1912) and not known in the Arcynopterygini and Diploperlini. The extreme reduction of the female subgenital plate is unique among the Perlodinae, though poorly produced subgenital plate is characteristic for the Pacific East Asian Megaperlodes and the Californian Salmoperla Baumann & Lauck, 1987. The presence of thoracic gills is characteristic for the Arcynopterygini. The new genus is the only Perlodini where thoracic gills are documented; however, gill vestiges are small and can be easily overlooked. Submental gills may both be present or lacking in all three tribes. In the Perlodini, more or less developed submental gills are present in Helopicus Ricker, 1952, Hydroperla, Isogenoides, Malirekus, Susulus, Tadamus, Yugus and the new genus. There are four genera of Perlodidae that are characterized by an entirely dark head and pronotum: Hemimelaena Klapálek, 1907, Levanidovia, Tadamus Ricker, 1952 and the new genus. Surprisingly, three of these are restricted to the southern temperate Pacific area of Asia and classified in the Perlodini, though they are apparently not closely related. Contrasting dark and pale wing pattern is rare among the Asian Perlodidae; besides the new genus, patterned wings are known for Megaperlodes tiunovi Teslenko, 2015 and Filchneria zhouchangfai (Chen, 2020), both of which belong to the Perlodes genus group sensu Zwick (1997). The enlarged costal field and unbranched Radial vein of both fore- and hindwings are unique among Perlodidae, and can be regarded as apomorphies. The enlarged costal area (and unusually large, rounded anal field of hindwing) is reminiscent of the Antarctoperlarian family Eustheniidae, but it surely does not reflect on phylogenetic relations with that basal family. The unbranched Radial vein of the female is unique among the Plecoptera. Etymology: The new genus is named after the Korean water god Habaek. Used as a noun, gender masculine., Published as part of Murányi, Dávid & Hwang, Jeong Mi, 2023, A new genus and species of Perlodinae (Plecoptera: Perlodidae) from Korea, pp. 138-150 in Zootaxa 5249 (1) on page 139, DOI: 10.11646/zootaxa.5249.1.8, http://zenodo.org/record/7685440, {"references":["Stark, B. P. & Szczytko, S. W. (1984) Egg morphology and classification of Perlodinae (Plecoptera: Perlodidae). Annales de Limnologie, 20 (1 - 2), 99 - 104. https: // doi. org / 10.1051 / limn / 1984029","Stark, B. P. & Szczytko, S. W. (1988) Egg morphology and phylogeny in Arcynopterygini (Plecoptera: Perlodidae). Journal of the Kansas Entomological Society, 61 (2), 143 - 160.","Ricker, W. E. (1943) Stoneflies of Southwestern British Columbia. Indiana University Publications Science Series, 12, 1 - 145.","Zwick, P. (1973) Insecta Plecoptera. Phylogenetisches System und Katalog. Das Tierreich, Berlin, 94, 1 - 465.","Kohno, M. (1946) Notes on two new genus of Plecoptera from Japan. Collecting and Breeding, 8 (3 - 5), 58 - 65. [in Japanese]","Ricker, W. E. (1952) Systematic studies in Plecoptera. Indiana University Publications Science Series, 18, 1 - 200.","Inada, K. (1996) Illustrated stonefly adults (Insecta, Plecoptera) of Hyogo Prefecture, Honshu, Japan, Part 1, Peltoperlidae and Perlodidae. Biology of Inland Waters, 11, 45 - 74. [in Japanese]","Shimizu, T., Inada, R. & Uchida, S. (2005) Plecoptera. In: Kawai, T. & Tanida, K. (Eds.), Aquatic insects of Japan, Manual with keys and illustrations. Tokai University Press, Kanagawa, pp. 236 - 290. [in Japanese]","Stark, B. P. & Stewart, K. W. (1982) Oconoperla, a new genus of North American Perlodinae (Plecoptera: Perlodidae). Proceedings of the Entomological Society of Washington, 84 (4), 746 - 752. https: // doi. org / 10.1093 / aesa / 75.1.84","Klapalek, F. (1904) Ueber die Europaeischen arten der Fam. Dictyopterygidae. Bulletin international de l'Academie des Sciences de Boheme, 13 (17), 1 - 10.","Frison, T. H. (1935) The stoneflies, or Plecoptera, of Illinois. Illinois Natural History Survey Bulletin, 20 (4), 281 - 471. https: // doi. org / 10.21900 / j. inhs. v 20.269","Klapalek, F. (1912) Plecopteres I. Fam. Perlodidae. Collections Zoologiques du Baron Edm. de Selys Longchamps, 4 (1), 1 - 66.","Baumann, R. W. & Lauck, D. R. (1987) Salmoperla, a new stonefly genus from Northern California (Plecoptera: Perlodidae). Proceedings of the Entomological Society of Washington, 89 (4), 825 - 830.","Klapalek, F. (1907) Evropsk druhy rodu Perla Geoffr. Vestnik Cesk akademie cisare Frantiska Josefa, 2 (16), 1 - 25.","Teslenko, V. A. (2015) A new species of Megaperlodes Yokoyama et al. 1990 (Plecoptera, Perlodidae) from the South of the Russian Far East. Zootaxa, 3904 (4), 553 - 562. https: // doi. org / 10.11646 / zootaxa. 3904.4.4","Chen, Z. T. (2020) Sinoperlodes, a new genus of subfamily Perlodinae (Plecoptera, Perlodidae) from coastal southeastern China. Zootaxa, 4779 (4), 584 - 594. https: // doi. org / 10.11646 / zootaxa. 4779.4.9","Zwick, P. (1997) Rauserella, a new genus of Plecoptera (Perlodidae), with notes on related genera. In: Landolt, P. & Sartori, M. (Eds.), Ephemeroptera & Plecoptera, Biology-Ecology-Systematics. MTL-Mauron + Tinguely & Lachat SA, Fribourg, pp. 489 - 496."]}

Habaek koreanus sp. n. (Figs. 1–22) Diagnosis: As for the genus. Type material. Holotype male (EMKU): SOUTH KOREA, Gangwon-do, Inje-gun, Girin-myeon, Bangdongri, Bangtaesan Natural Recreation Forest, 37°54′29.57″ N 128°24′25.14″ E, alt. 690 m, 11.V.–21.VI.2019, Malaise trap, leg. Daseul Ham, Sunghwan Park. Paratypes: same data as holotype: 1 female (EMKU); Gangwon-do, Pyeongchang-gun, Jinbu-myeon, Odaesan, 37°47′5.67″ N, 128°34′16.97″ E, alt. 830 m, 1.VI.–14.VII.2019: 1 male (NIBR, NIBRIN0000886471); Gangwon-do, Pyeongchang-gun, Jinbu-myeon, Bukdae Mireukam Temple, Odaesan, 37°48′12.82″ N, 128°34′03.02″ E, alt. 1296 m, 1.V.–29.V.2020, Malaise trap, leg. Jeong Mi Hwang, Ji Hyoun Kang, Daseul Ham, Sunghwan Park: 2 males, 2 females (EMKU); same locality, 23.VI.–28.VII.2020, Malaise trap, leg. Jeong Mi Hwang, Ji Hyoun Kang, Daseul Ham, Sunghwan Park: 12 males, 9 females (EMKU), 1 male, 1 female (EKCU); Gangwon-do, Pyeongchang-gun, Jinbu-myeon, Odaesan, 37°47′34.58″ N, 128°33′37.97″ E, alt. 929 m, 29.V.–23.VI.2020, Malaise trap, leg. Jeong Mi Hwang, Ji Hyoun Kang, Daseul Ham, Sunghwan Park: 2 females (EMKU); Gangwon-do, Pyeongchang-gun, Jinbu-myeon, Odaesan, 37°47′35.47″ N, 128°33′43.19″ E, alt. 941 m, 30.V.–23.VI.2020, Malaise trap, leg. Jeong Mi Hwang, Ji Hyoun Kang, Daseul Ham, Sunghwan Park: 1 male (EMKU), 1 male, 1 female (EKCU); Gangwon-do, Pyeongchang-gun, Jinbu-myeon, Odaesan, 37°45′19.09″ N, 128°34′22.24″ E, alt. 822 m, 29.V.–23.VI.2020, Malaise trap, leg. Jeong Mi Hwang, Ji Hyoun Kang, Daseul Ham, Sunghwan Park: 1 male (EMKU); Gangwon-do, Pyeongchang-gun, Jinbu-myeon, Odaesan, 37°47′4.20″ N, 128°33′42.31″ E, alt. 822 m, 29.V.–23.VI.2020, Malaise trap, leg. Jeong Mi Hwang, Ji Hyoun Kang, Daseul Ham, Sunghwan Park: 1 male, 3 females (EMKU); Gangwon-do, Gariwangsan Natural Recreation Forest in Hoedong-ri, Jeongseon-eup, Jeongseon-gun, 37°25′4.80″ N 128°31′27.51″ E, alt. 697m, 26.V.–20.VI.2020, leg. Daseul Ham, Sunghwan Park: 2 females (EMKU). Description: Medium sized Perlodinae, males brachypterous, females slightly brachypterous (Figs. 17–18). Forewing length: holotype male 7.5 mm, male paratypes 6.5–7.5 mm, female paratypes 10.0–11.0 mm; body length: holotype male 14.0 mm, male paratypes 12.0– 12.5 mm, female paratypes 15.0– 15.5 mm. Sexual dimorphism regards size, terminalia and different wing length: female wings terminate above tip of abdomen while male wings do not cover terminal segments; shortened male wing has less crossveins but branching Radial vein. Setation generally short and dense. General colour dark brown, contrasting pale pattern on wings and appendages. Head and pronotum entirely dark brown; tentorial callosities and M-line hardly visible, occiput with well developed but not contrasting rugosities (Fig. 11). Eyes and ocelli black and small, ocelli located far from each other, in a regular triangle.Antenna as long as the length of body, with more than 45 slightly clubbed antennomeres. Scape bicolored: base and inner edge dark brown, apex and outer edge light brown; pedicel and the next few antennomeres are light brown, gradually darkened towards dark brown two thirds of the antennae. Palpi brown, mouthparts mostly dark brown but glossa, paraglossa and apex of labrum contrastingly pale; vestigial submental gills present (Fig. 4). Pronotum rectangular, about as wide as long, edges rounded; rugosities numerous and small. Meso- and metanotum dark brown with whitish membranes around the wing base. Forewing with contrasting pattern: costal area and anal field shiny dark brown with metallic tint, the rest of the remigium off-white; venation brown, darker in the costal area. Hindwing is less contrasting, mostly brown with dark brown costal area (Figs. 2–3). Ventral surface of thorax contrasting: membranous areas white, while sclerotized plates brown to dark brown. Legs long and slender; tibiae distinctly longer than femora, and even more slender; claws symmetrical, arolium large. Femora dark brown with contrasting pale apical ring. Basal third of tibiae dark brown, then gradually lightened towards pale brown apical third. Tarsi light brown to brown but apical half of metatarsi dark brown. Ventral sclerites of thorax (Fig. 1): Prothorax: cervical sclerites large, with bud-like gill vestiges; rounded presternum fused with small basisternum,both with a longitudinal median ridge;precoxal bridge vestigial,eutrochantin well developed; furcasternum large and triangular, fused with basisternum, postcoxal bridge and medially ridged postfurcasternum; the furcasternal area similar to the mesothoracic furcasternum–furcasternal arm–furcasternal pit complex. Mesothorax: anterior thoracal bud-like gill vestiges present; spinasternum vestigial, presternum large and triangular, fused with the small, stripe-like basisternum; trochantin stripe-like; furcasternum large and triangular, fused with basisternum; furcal pit (furcasternal arm) connecting anteriorly to arm of mesosternal ridge that continued in a long stem (furcasternal pit); areas between furcal pit and stem sclerotized but hardly delimited. Metathorax: anterior thoracal bud-like gill vestiges present; spinasternum vestigial, presternum large and triangular, fused with the small, stripe-like basisternum; trochantin stripe-like; furcasternum large and quadrangular, fused with basisternum; furcal pit connecting to lateral margin of furcasternum; sternum I fused with furcasternum. Wing venation (Figs. 2–3, 5–6): Forewing: Costal field enlarged, with many irregular, often forked or interrupted crossveins between Costa and irregular Subcosta, humeral crossvein not clearly distinguishable. Subcosta joins the unbranched Radial vein before single radiomedial crossvein in the female, well after the branching of Radial vien in the male; there are one or two crossveins between Subcosta and Radial vein, further two between Costa and Radial vein, stigma is lacking. Medial vein originates independently from Radial vein but its base is obscure, the two veins are distinctly separated well before arculus; Medial vein branches into an irregular network shortly after arculus in the female, with several crossveins, and four longitudinal veins reaching wing margin. There are 3–5 mediocubital crossveins besides arculus. Cu1 and Cu2 branching before arculus, cubital cell quadrangular, there are 8–9 cubital crossveins in the female, 3–4 in the male; Cu1 ends about the position of Radial vein ending, last 2–3 crossveins end directly in wing margin, Cu2 ends about the position of Subcosta ending. Cubital vein and A1 basally fused, branches at the anal crossvein, cubitoanal crossvein distinct in the female but lacking in the male; A1 strongly curved after the crossveins, ends about half of wing length. There are three further anal veins besides A1; A2 and A3 can be connected with a crossvein, A4 is very short. Hindwing: Enlarged costal field, Costa, Subcosta and Radial vein network is generally similar to forewing, but humeral crossvein can be identified more distinctly. Origin of Medial vein separated from Radial vein, similarly to forewing, but irregular network of its branching is with less crossveins. There are two mediocubital crossveins besides arculus. Cu1 and Cu2 branching before arculus, cubital cell quadrangular but short, there are 5–6 cubital crossveins. Cubitoanal crossvein short and connected to a basal anal cell, there is an additional crossvein between Cu2 and A1 well after half wing length. Anal field very large and as long as the remigium, the fold of the wing extending between the parallel Cu2 and A1. Additional A veins are 7–8, branching irregularly, the last Anal vein gives irregular crossveins towards wing margin. Male abdomen (Figs. 7–8, 19–20): Segments I–II are fully divided into terga and sterna by pleura, segment III with shallow posterior notch of pleura, rest of the segments are entire. Terga I–III with wide, lightly sclerotized medial field, tergum IV with small medial field, terga V–IX entirely sclerotized and unmodified, dark brown; terga I–IX with entire antecosta, lack sensilla basiconica. Tergum X full divided into hemiterga; mostly dark brown, apical portions lighter, lack sensilla basiconica. Hemiterga triangular with rounded apex, the apical halves are converging in dorsal view, and elevated in lateral view; the wide basal anchor of epiproct divides hemiterga in the basal third by more than half of segment width, apices of hemiterga divided by a gap as wide as one fifth of the segment width. Sterna I–VIII brown, gradually darkening towards the apical segments, lacks vesicle. Sternum IX forms a well developed, rounded subgenital plate, covering sternum X and most of paraproct; sternum X widely divided medially. Paraproct dark brown, triangular with narrowed apical half, ending in a blunt apex; eversible portion is lacking. Cercus as long as the length of body, with 22–24 cylindrical segments, apical ones are rather elongated; basal 4–5 cercomeres yellowish, the rest is gradually darkened towards dark brown apical half of the cercus, but each cercomere with pale apical ring. Epiproct (Figs. 7–8, 12–14, 19–20): Basal anchor large and wide, connected to large, posteriorly directed lateral arms with raised tip, stretching basal membranes.The lever arm long and curved, rather wide in dorsal view, separated from the complex epiproct tip. The epiproct tip is shaped like an elongated 8 in dorsal, straight stripe-like in caudal, and half moon shaped in lateral view; most of the surface is lightly coloured but with a wide, lateral dark stripe and a dark dorsal ridge; the apex is arrow-shaped, tip slightly down curved in lateral view, and basally connected to rounded membranous portion. Paragenital plate large and bears ventral projection; stripe-like and slightly curved in dorsal view, dorsal portion darker in lateral view and with down curved apical portion, ventral projection has caudal edge perpendicular to the dorsal portion. Lateral stylet small, not fused and hidden in the cowl in resting condition of the epiproct; curved and stripe-like, apex with a curved, acute tooth. The cowl is densely covered by setae on most of its surface, with the exception of the dorsal surface basally to lateral stylets; bears a distinct, wide field of dense, tiny golden spikes between the stretched lateral stylets, seen only in fully everted position. In everted condition of the epiproct, the lateral arms of basal anchor raised dorsad, as well the rather enlarged cowl and epiproct tip that is fully exposed. Paragenital plates only slightly change posture, holding tight the base of the cowl. The lateral stylets are turning outwards by the base of the epiproct tip, stretching the apex of the cowl into two small, apicolateral lobes, and exposing the field of golden spines in front of the epiproct tip. The everted cowl has no other lobes, its shape is blunt conical in lateral, and rectangular in caudal view. Aedeagus (Figs. 15–16): Shape and lobes of the fully everted aedeagus remained unknown, but surface armatures and certain lobes around the armatures were clearly seen after dissection. On the ventral surface, golden brown scales are forming a large T-shaped loop; the scales are irregular small plaques with raised flanges. Adjacent to the upper part of the T, a pair of small lobules present, while the lower stem of the T is connected to a larger lobe forming an upside down C, bearing a small patch of scales beneath the stem of the T. Both the paired lobules and the lower lobe bear minute colourless spicules, scarce spinules distributed also on the surface beneath lower lobe; the surface lateral and above to the T is rarely wrinkled. The dorsal surface is armed with three small patches of golden scales similar to the ventral surface but no spicules; the upper two, elongated patches are positioned on inverting lobes, while the third, rounded patch is positioned medially on a rounded lower lobe. Female abdomen (Figs. 9–10): Segments I–II are fully divided into terga and sterna by pleura, segment III with shallow posterior notch of pleura, rest of the segments are entire. Terga unmodified, uniformly brown but apical two segments are darker, all with entire antecosta. Sterna I–VII brown, gradually darkening towards the apical segments. Subgenital plate reduced to a very slightly protruded, widely rounded posterior margin of sternum VIII, slightly bulging in lateral view; sternum VIII dark brown, with an inconspicuous longitudinal pale stripe medially. Sterna IX–X unmodified, dark brown but sternum IX with a mediobasal paler area. Paraproct dark brown with paler tip, elongated and narrow triangular, ending in a blunt apex. Cercus nearly as long as the length of body, with 24 cylindrical segments, apical ones are rather elongated; basal 3–4 cercomeres yellowish, the rest is gradually darkened towards dark brown apical two thirds of the cercus, but each cercomeres with narrow, pale apical ring. Egg: No fully matured eggs are available, but one of the females contains several dozens of nearly matured eggs. These are spherical, elongated oval with 0.3–0.4 mm length and 0.2–0.3 mm diameter. The chorion is golden light brown, apparently with dense punctuations of FCIs. No traces of collar or rims can be recognized. Larva: unknown. Affinities: As for the genus. Ecology and distribution: The species was caught by Malaise traps during May, June and July. The traps were set at elevations ranging from 700 to 1300 m, along medium sized forest streams with fast flow and mixed rocky and sandy substrate (Fig. 21). The known localities are located in the Thebaek ranges, in three counties of Gangwon province (Gangwon-do): Inje-gun, Pyeongchang-gun, Jeongseon-gun (Fig. 22). Etymology: The name koreanus refers to the Korean Peninsula where this remarkable stonefly was found. Used as a noun adjective, gender masculine., Published as part of Murányi, Dávid & Hwang, Jeong Mi, 2023, A new genus and species of Perlodinae (Plecoptera: Perlodidae) from Korea, pp. 138-150 in Zootaxa 5249 (1) on pages 140-146, DOI: 10.11646/zootaxa.5249.1.8, http://zenodo.org/record/7685440

The current study aimed to examine the responses of Ephemeroptera, Plecoptera and Trichoptera (EPT) taxa to the major physicochemical (temperature, pH, electrical conductivity, dissolved oxygen, biological oxygen demand, chemical oxygen demand) and hydro-morphological (stream width, water depth and current speed) factors affecting their abundance, richness and distribution. Immature insects of Ephemeroptera, Plecoptera and Trichoptera were sampled as well as water during eight seasonal surveys conducted at 19 stations in Martil basin from spring 2016 to spring 2018. In total, 10,370 individuals belonging to 22 families, 36 genera and 48 species were collected during the study period. Baetidae, Perlodidae and Hydropsychidae were the most abundant and diversified families, respectively for each order. Significant biotypological differences of the EPT assemblages were observed among stream types relating to their spatial distribution, hydrological features and pollution levels. However, species richness and abundance were positively correlated with DO, pH and altitude and negatively correlated with temperature and the other parameters measured. The taxonomic list compiled following our recent investigations includes six new species for the Martil basin enabling them to expand their range in northern Morocco. Furthermore, the biogeographical analysis shows that EPT taxa from Martil basin are essentially composed of Mediterranean elements, with a high proportion of Ibero-Maghrebian endemic species. Moreover, this study provides a basis for further studies to analyze the distribution patterns of EPT taxa through multi-dimensional approaches.

An investigation report of stonefly fauna in Benxi Manchu Autonomous County, Liaoning Province, northeast China (used to be called Manchuria, now includes Liaoning, Jilin, Heilongjiang Provinces and parts of Inner Mongolia, which are adjacent to the Russian Far East and the Korean Peninsula). Materials were studied with field observation in 2018 and 2019. This paper records five families, nine genera and 14 species of stoneflies from Taizi River, Liaoning Province. Nine species have been recorded for the first time in China and the biology of several common species is also reported for the first time.

Perlodinella microlobata Wu, 1938 http://lsid.speciesfile.org/urn:lsid: Plecoptera.speciesfile.org:TaxonName:737 Perlodinella microlobata: Wu, 1938, 55; Yang & Li, 2018, 52. Adult habitus: See Wu (1938). The original illustrations of terminalia of type materials are shown in Fig. 1. Supplementary description: In the specimen from Qinghai, male coloration varies from dark brown to black (Fig. 2). Head mostly black, yellow spots only present between the hind ocelli and median occiput (Fig. 3A). Pronotum with a yellow median band (Fig. 3A). Posterior margin of male tergum 9 with a median swelling covered by a horizontal oval patch of sensilla basiconica; tergum 10 caudally constricted and extended upwards, apex bearing a triangular patch of sensilla (Fig. 4A–B). Paraproct subtriangular, inner base with darker spot; apex membrane but without obvious apical sclerite (Fig. 4C). Epiproct membranous, with a few sparse dorsal spines and dense ventral asperities (Figs. 3B–C, 5A–C). Penis membranous, bearing dense basal and subterminal spines (Fig. 5D). For the specimen from Sichuan, the male coloration is brownish (Fig. 6) and the pattern of head is similar with the male from Qinghai (Fig 7A). The posterior margin of tergum 9 and median sternum 9 have paler coloration (Figs. 7B–C, 8A–B). Paraprocts are darker on the base (Fig. 8C). Because these materials have been soaked in 75% alcohol for more than 10 years, the body color is much lighter than the fresh specimen from Qinghai. Females from Sichuan have coloration patterns that are similar to males (Figs. 9, 10A). The subgenital plates is sclerotized medially (Fig. 10B–C), with an outline same to the illustrations of Wu (1938) (Fig. 1). Nymph: Unknown. Mature egg: General color brown. Wide and trilateral, with three longitudinal ridges; surface except the ridges covered by dense asperities; each side of the egg with 3–6 short transverse rows of micropyles (Fig. 11A, C). Collar is a short brown ring, cylindrical with a broad base. Anchor flat, without massive stem, base evenly covered with large globular bodies (Fig. 11A–B). Material examined: 1 male, China, Qinghai Province, Haixi Mongol and Tibetan Autonomous Prefecture, Tianjun County, 2021-VII-13, 37.568689°N, 98.657256°E, 3545 m, leg. Huo Qing-Bo (ICYZU). 1 male, 3 females, China, Sichuan Province, Garze Tibetan Autonomous Prefecture, Shiqu County, 2009-VI-29, 32.838611°N, 98.376944°E, 4200 m, leg. Qian Yu-Han (ICYZU). Distribution: China (Gansu, Qinghai, Sichuan, Shanxi, Liaoning) (Wu 1938, Yang & Li 2018). Remarks: In Tianjun County, the sole male of P. microlobata was collected with several P. kozlovi Klapálek, 1912 and Tibetisoperla wangluyui together in the same environment. Perlodinella microlobata may emerge much later than P. kozlovi in general. In addition, the specimens of P. microlobata from Shanxi and Liaoning mentioned by Wu (1938) were all females, without collecting any associated males. Considering that the female features may be variable (Huo et al. 2020, 2022), the validity of the distribution record in Shanxi and Liaoning is doubtful and needs further study., Published as part of Huo, Qing-Bo, Rehman, Abdur, Du, Yu-Zhou & Chen, Zhen-Ning, 2022, Rediscovery of Perlodinella microlobata Wu, 1938, with notes on Tibetisoperla sclerotica Yan, Chen, Bozdogan & Li, 2022 (Plecoptera: Perlodidae), pp. 26-34 in Zootaxa 5205 (1) on pages 27-32, DOI: 10.11646/zootaxa.5205.1.2, http://zenodo.org/record/7285772, {"references":["Wu, C. F. (1938) The stoneflies of China (Order Plecoptera). Bulletin of the Peking Society of Natural History, 13 (1), 53 - 87.","Yang, D. & Li, W. H. (2018) Species catalogue of China. Vol. 2. Animals, Insecta (III), Plecoptera. Science Press, Beijing, 71 pp.","Klapalek, F. (1912) Plecopteres I. Fam. Perlodidae. Collections Zoologiques du Baron Edm. de Selys-Longchamps, 4 (1), 1 - 66.","Huo, Q. B., Chen, Z. N., Kong, X. B. & Du, Y. Z. (2020) New records and a confirmation of three perlodid species in China, with additional notes and images of Rauserodes epiproctalis (Zwick, 1997) (Plecoptera: Perlodidae). Zootaxa, 4808 (3), 455 - 474. https: // doi. org / 10.11646 / zootaxa. 4808.3.3"]}

Huo, Qing-Bo, Rehman, Abdur, Du, Yu-Zhou, Chen, Zhen-Ning (2022): Rediscovery of Perlodinella microlobata Wu, 1938, with notes on Tibetisoperla sclerotica Yan, Chen, Bozdogan & Li, 2022 (Plecoptera: Perlodidae). Zootaxa 5205 (1): 26-34, DOI: https://doi.org/10.11646/zootaxa.5205.1.2

A remarkable new species of Isoperla Banks, 1906, Isoperla chongxui sp. nov. from Henan Province of China is described and illustrated. The new species is characterized by the conspicuous abdominal tergal processes in males. Comparisons are made between the new species and its congeners. New descriptions and illustrations are provided for Isoperla kozlovi Zhiltzova, 1972 from northeastern China. Isoperla fengi Wu & Claassen, 1934 and Isoperla curvispina (Wu, 1938) are considered nomen dubia due to lost types and poor original descriptions.

A new brachypterous species of Perlodidae, Filchneria urumqiensis sp. n., from Xinjiang Uygur Autonomous Region of northwestern China is described and illustrated based on male, female and eggs. Mo...

Perlodinella Klapálek, 1912 is a small stonefly genus in the Palearctic areas of China and its biodiversity is underestimated. This paper reports a new species of Perlodinella, Perlodinella shennongjia sp. nov. in the Dajiuhu National Wetland Park of Shennongjia Forestry District, Hubei Province, central China. The description and illustrations of the new species are provided, based on male adults, female adults and eggs. The new species can be distinguished from its congeners by the characters of male and female genitalia and the egg structure.

Perlodinella epiproctalis (Zwick, 1997) (comb. nov.) Rauserella epiproctalis: Zwick, 1997: 489. Rauserodes epiproctalis: Zwick, 1999: 168; Yang & Li, 2018: 52; Huo, Chen, Kong & Du, 2020: 455. Adult habitus: See Zwick (1997, 1999) and Huo et al. (2020). Nymph: Mature male body length 17–20 mm (n = 3). Dorsum gray to olive green with some pale patterning; ventrally paler grey (Figs. 24–25). Body covered by short colorless hairs. Head with a large pale spot in front of the anterior ocellus extending onto the clypeus; M-line indistinct; interocellar area brown with small pentagonal, central pale spot, closed posteriorly; occiput with a pair of oval pale spots around the epicranial stem bordered by sinuate brownish rows behind each eye (Fig. 24A). Submental gills are short (Fig. 24B). Mandible bidentate, with 2 apical teeth and 3 subapical teeth; a patch of 15–18 hairs present ventral to the subapical teeth (Fig. 25A). Lacinia bidentate, apically narrow, basal half expanded; 12–14 submarginal setae present; 2–3 setae present ventral to the base of the subapical tooth and 2–3 setae at the juncture of the apical teeth (Fig. 25B). Paraglossae flat and fingerlike; glossa wide and semicircular, apex slightly truncated, with thick fine hairs (Fig. 25C). Thoracic segments rectangular with wide, pale, median stripes. Male wingpads very short. Legs with few scattered brown spinules on surface (Fig. 24A). Abdominal terga with two rows of large, oval, pale paramedial spots; spots are indistinct on tergum 10 (Fig. 24C–D). All abdominal segments have a few short, stout spinules that become more numerous on the sterna 6–10. Epiproct is already visible on mature nymphs (Fig. 25D). Paraprocts short, apex rounded (Fig. 24C–D). Cerci with dorsal fringe of fine, silky, colorless hairs; each cercal segment with an apical whorl of short brown setae (Fig. 25E). Mature female body length 22–25 mm (n = 5). Coloration and morphology similar to male (Fig. 26). Wingpads length regular (Fig. 26A). A horizontal sclerite is present on the posterior margin of sternum 8 of female (Fig. 26D). Egg: General color dark brown to black. Subglobular, with three obscure longitudinal ridges. Collar slender and more sclerotized, cylindrical with a broad base. Anchor mushroom-shaped, evenly covered with small globular bodies. Each side of the egg with 5-6 short transverse row of micropyles (Fig. 27). Material examined: The same collection sites were recorded in Huo et al. (2020); 8 males, 4 females, 10 nymphs, China, Qinghai Province, Haibei Tibetan Autonomous Prefecture, Menyuan Hui Autonomous County, Laohugou, 3549 m, 101.582778°E, 37.585556°N, 2021-VII-4, leg. Huo Qing-Bo, Zang Hao-Ming, Shen Rong-Rong; 11 males, 9 females, 8 nymphs, Qinghai Province, Qilian County, Longkong, 3425 m, 100.680833°E, 38.067500N, 2021-VII-5, leg. Huo Qing-Bo, Zang Hao-Ming. Distribution: China (Qinghai). Remark: Perlodinella epiproctalis is widespread in most of the basin of Datong River, western Qilian Mountains (Qilian, Menyuan and Datong counties), and is also the only Perlodinella species known to emerge in this area during July and August., Published as part of Huo, Qing-Bo, Du, Yu-Zhou, Zwick, Peter & Murányi, Dávid, 2022, Notes on Perlodinella Klapálek, 1912 (Plecoptera: Perlodidae) with a new species and a new synonym, pp. 378-396 in Zootaxa 5162 (4) on pages 390-391, DOI: 10.11646/zootaxa.5162.4.3, http://zenodo.org/record/6810221, {"references":["Zwick, P. (1997) Rauserella, a new genus of Plecoptera (Perlodidae), with notes on related genera. In: Landolt, P. & Sartori, M. (Eds.), Ephemeroptera & Plecoptera, Biology - Ecology-Systematics. MTL-Mauron + Tinguel Lachat SA, Fribourg, pp. 489 - 496.","Zwick, P. (1999) Notes on Plecoptera (22) Rauserodes nom. n. replacement name for Rauserella Zwick (Plecoptera: Perlodidae). Aquatic Insects, 21 (3), 168. https: // doi. org / 10.1076 / aqin. 21.3.168.4518","Yang, D. & Li, W. H. (2018) Species Catalogue of China. Vol. 2. Animals, Insecta (III), Plecoptera. Science Press, Beijing, 71 pp.","Huo, Q. B., Chen, Z. N., Kong, X. B. & Du, Y. Z. (2020) New records and a confirmation of three perlodid species in China, with additional notes and images of Rauserodes epiproctalis (Zwick, 1997) (Plecoptera: Perlodidae). Zootaxa, 4808 (3), 455 - 474. https: // doi. org / 10.11646 / zootaxa. 4808.3.3"]}

Genus Perlodinella Klapálek, 1912 Perlodinella: Klapálek, 1912: 28; Wu, 1938: 28; 1973: 98; Illies, 1966: 388; Li & Murányi, 2015: 48; Yang & Li, 2018: 52; Chen, 2019: 297; Huo, Chen, Kong & Du, 2020: 455. Rauserella: Zwick, 1997: 489. Rauserodes: Zwick, 1999:168. (syn. nov.) Revised diagnosis: Body color and wings possibly are sexually dimorphic. Male tergum 10 narrow, greatly raised (Klapálek, 1912); epiproct developed, eversible and membranous; paraproct possibly eversible, membranous in apex; cercus with sharp teeth on basal segments., Published as part of Huo, Qing-Bo, Du, Yu-Zhou, Zwick, Peter & Murányi, Dávid, 2022, Notes on Perlodinella Klapálek, 1912 (Plecoptera: Perlodidae) with a new species and a new synonym, pp. 378-396 in Zootaxa 5162 (4) on page 379, DOI: 10.11646/zootaxa.5162.4.3, http://zenodo.org/record/6810221, {"references":["Klapalek, F. (1912) Plecopteres I. Fam. Perlodidae. Collections Zoologiques du Baron Edm. de Selys-Longchamps, 4 (1), 1 - 66.","Wu, C. F. (1938) The stoneflies of China (Order Plecoptera). Bulletin of the Peking Society of Natural History, 13 (1), 53 - 87.","Wu, C. F. (1973) New species of Chinese stoneflies (order Plecoptera). Acta Entomologica Sinica, 16 (2), 97 - 118.","Illies, J. (1966) Katalog der rezenten Plecoptera. Das Tierreich, Berlin, 82, 1 - 632.","Li, W. H. & Muranyi, D. (2015) A remarkable new genus of Perlodinae (Plecoptera: Perlodidae) from China, with remarks on the Asian distribution of Perlodinae and questions about its tribal concept. Zoologischer Anzeiger, 259, 41 - 53. https: // doi. org / 10.1016 / j. jcz. 2015.10.003","Yang, D. & Li, W. H. (2018) Species Catalogue of China. Vol. 2. Animals, Insecta (III), Plecoptera. Science Press, Beijing, 71 pp.","Chen, Z. T. (2019) Perlodinella mazehaoi sp. nov., a new species of Perlodidae (Plecoptera) from Inner Mongolia of China. Zootaxa, 4651 (2), 297 - 304.","Huo, Q. B., Chen, Z. N., Kong, X. B. & Du, Y. Z. (2020) New records and a confirmation of three perlodid species in China, with additional notes and images of Rauserodes epiproctalis (Zwick, 1997) (Plecoptera: Perlodidae). Zootaxa, 4808 (3), 455 - 474. https: // doi. org / 10.11646 / zootaxa. 4808.3.3","Zwick, P. (1997) Rauserella, a new genus of Plecoptera (Perlodidae), with notes on related genera. In: Landolt, P. & Sartori, M. (Eds.), Ephemeroptera & Plecoptera, Biology - Ecology-Systematics. MTL-Mauron + Tinguel Lachat SA, Fribourg, pp. 489 - 496.","Zwick, P. (1999) Notes on Plecoptera (22) Rauserodes nom. n. replacement name for Rauserella Zwick (Plecoptera: Perlodidae). Aquatic Insects, 21 (3), 168. https: // doi. org / 10.1076 / aqin. 21.3.168.4518"]}

Perlodinella tibetensis Huo & Du sp. nov. Adult habitus: General body color dark brown to black (Figs. 12, 17). Triocellate, anterior ocellus small, distance between posterior ocelli almost equal to width of the eyes. Head black, with a small yellowish spot around each ocellus and an oval yellowish spot between the posterior ocelli. Pronotum black with pale rugosities, without any median stripe (Figs. 13, 19). Legs brownish. Abdominal segments dark brown to black. Male: Micropterous, forewing length 3–4 mm, hindwing length 2–3 mm, body length 11–13 mm (n = 6). Abdomen slightly sclerotized, bearing long yellowish hairs. Terga 8–9 without sensilla patches or sclerotized dots (Figs. 14–16). Tergum 10 yellowish and smooth medially; posterior margin heavily sclerotized, curved upward; sensilla basiconica arranged in a V-shape on the posterior margin (Figs. 15E–F, 16C). Paraproct divided into a subtriangular apical membrane with a typical sclerotized band, and a basic sclerite; apical half of the sclerite slender, hooklike, and curved outward, sharp on apex (Figs. 15D, 16C–D). The membranous epiproct bears sparse spines, divided into three isometric spherical lobes. The paired basal lobe swollen; apical lobe blunt apically (Fig. 14C–E). Penis (partially everted) membranous, dorsal base bearing small asperities (Fig. 15G). Female: Coloration similar to males (Fig. 17). Macropterous, forewing length 14–15 mm, hindwing length 12–13 mm, body length 13–15 mm (n = 12) or brachypterous, forewing length 9 mm, hindwing length 7 mm, body length 14 mm (n = 1). Yellow spots between the hind ocelli and median occiput, at least two patterns present on females (see Fig. 19). Wings pale yellowish to smoky gray, translucent; veins dark brown (Figs. 17–18). Abdomen dark brown; sterna yellowish laterally, with a pair of lateral dark spots; subgenital plate short and sclerotized, variable; median posterior margin heavily sclerotized and bilobate. There are four morphs of female subgential plates (Figs. 20A–D), with the posterior margin with a small median extension (Fig. 20A), posterior margin truncated (Fig. 20B), posterior margin with small lateral processes (Fig. 20C), and posterior margin M-shaped, the lateral process on each side longer and narrowed (Fig. 20D). Nymph: Unknown. Egg: General color brown. Slender, trilateral, with three longitudinal ridges (Fig. 21). Collar is a short brown ring, cylindrical with a broad base. Anchor mushroom-shaped with massive stem, evenly covered with more small globular bodies. Each side of the egg with 4–6 short transverse rows of micropyles. Under the observation by optical scopes, the egg of P. tibetensis has no obvious visible difference in shape or structure from P. kozlovi (Fig. 21 A–D) except the small globular bodies near the basic anchor are darker than others (Fig. 21 A). Etymology: The scientific name is a Latin adjective derived from the name of Tibet where it is the most widespread Perlodinella known in this area. Diagnosis: The new species can be distinguished by the completely black pronotum, the absence of sensilla basiconica on male tergum 9, V-shaped arrangement of sensilla basiconica on male tergum 10, and the outwardcurving male paraprocts. Type materials: Holotype: 1 male, Tibet Autonomous Region, China, XZZB-20-66, Longdangbo, Qiongguo Township, Zhongba County, 2020-VII-23, 29.769472°N, 83.926753°E, 4556 m. Paratypes: 4 males, 3 females, the same locality and data as the holotype; 2 males, 3 females, XZRT-20-79, Rutog Town, Rutog County, 2020-VII-25, 33.302156°N, 79.691631°E, 4288 m; 1 male, 2 females, XZGE-20-83, Jiamu River (riverside grassland, under the rocks), Shiquanhe Town, Gar County, 2020-VII-27, 32.436056°N, 80.203581°E, 4340 m; 1 female, XZGE-20-84, Yanhu Township, Gar County, Tibet 301 Provincial Highway North (Riverside grassland), 2020-VII-27, 32.403392°N, 82.407339°E, 4344 m; 7 males, 5 females, XZCQ-20-96, Meiduo Village, Quluo Township, Coqen County (riverside grassland, under the rocks), 2020-VII-29, 30.655397°N, 85.131839°E, 4751 m. Leg. Wang LuYu, Liu Piao, Yuan Tao, Hou Yan-Meng. Distribution: China (Tibet). Remarks: Adults of Perlodinella tibetensis occur in groups under rocks along the shoreline (Fig. 22). This species is distributed in the southwestern Tibet, while P. kozlovi only known in the junction of eastern Tibet with Sichuan and Qinghai (Fig. 23)., Published as part of Huo, Qing-Bo, Du, Yu-Zhou, Zwick, Peter & Murányi, Dávid, 2022, Notes on Perlodinella Klapálek, 1912 (Plecoptera: Perlodidae) with a new species and a new synonym, pp. 378-396 in Zootaxa 5162 (4) on pages 384-386, DOI: 10.11646/zootaxa.5162.4.3, http://zenodo.org/record/6810221

Perlodinella kozlovi Klapálek, 1912 Perlodinella kozlovi: Klapálek, 1912: 28; Wu, 1938: 28; 1973: 98; Claassen, 1940: 116; Ricker, 1952: 138; Illies, 1966: 388; Li & Murányi, 2015: 49; Yang & Li, 2018: 52. Description and data: See Klapálek (1912) and Wu (1938). Original illustration (Fig. 1) and type series of this species (Figs. 2–3) are shown below. Supplementary description: Male and female macropterous. General body color black, except ventral head and sides of female abdomen yellowish (Fig. 4). Yellow spots present between the hind ocelli and median occiput, sometimes also on clypeus (at least four patterns in male and two patterns in female, see Figs. 5, 9). Antennae are brown, palpi dark brown. Pronotum with a yellow median band. Wings brown, veins dark brown, sometimes slightly asymmetrical (Fig. 6). Legs and cerci mostly dark brown. Male: Terga 8–9 slightly sclerotized medially. Median hairs on tergum 8 thick and long. Tergum 9 bearing two sparse patches of sensilla, and a pair of small, sclerotized dots near the lateral margin. Tergum 10 sclerotized, caudally constricted and extended upwards, bearing multiple sensilla basiconica of similar size. Paraprocts divided into a basal sclerite and a subtriangular, eversible apical membrane covered with fine spines. Apical half of the sclerite slender, fingerlike, and straight, with a hook at tip (Figs. 3, 7–8). Epiproct membranous, with small, round, dorsal process on apex; ventral surface with asperities in a patch (Fig. 8B–C). Penis membranous, base with a pair of short arms bearing fine cuticular asperities; median part swelling and bearing larger asperities; apex bilobate, smooth on surface (Fig. 8C–E). Female: Coloration similar to males (Figs. 2, 4). There are two morphs of female subgenital plates, with the posterior margin either truncate (Fig. 9C) or M-shaped (Fig. 9D). Nymph: Unknown. Egg: General color brown to dark brown. Slender, trilateral, with three longitudinal ridges (Fig. 10). Collar is a short brown ring, cylindrical with a broad base. Anchor mushroom-shaped with massive stem, evenly covered with small globular bodies. Each side of the egg with 3–6 short transverse rows of micropyles. Material examined: Lectotype male (present designation), paralectotypes 1 male, 2 females, China, Sichuan Province, Kham area, Yangtze River Basin, Yalong River, approximately 33°N, 98°E (see Li & Murányi 2015), 1901-IV, leg. P. K. Kozlov (NMP); 18 males, 25 females, China, Qinghai Province, Haixi Mongol and Tibetan Autonomous Prefecture, Tianjun County, 2021-VII-13, 37.56869°N, 98.65726°E, 3545 m. Leg. Huo Qing-Bo, Zang Hao-Ming, Wang Ya-Meng, Sun Chen-Tao, Li Cong (ICYZU). Distribution: China (Tibet, Qinghai and Sichuan). Remarks: Specimens were found in groups under rocks along the broad river shoreline (Fig. 11). Tibetisoperla wangluyui Huo & Du, 2021 was also collected together with this species at the same time and environment., Published as part of Huo, Qing-Bo, Du, Yu-Zhou, Zwick, Peter & Murányi, Dávid, 2022, Notes on Perlodinella Klapálek, 1912 (Plecoptera: Perlodidae) with a new species and a new synonym, pp. 378-396 in Zootaxa 5162 (4) on pages 379-383, DOI: 10.11646/zootaxa.5162.4.3, http://zenodo.org/record/6810221, {"references":["Klapalek, F. (1912) Plecopteres I. Fam. Perlodidae. Collections Zoologiques du Baron Edm. de Selys-Longchamps, 4 (1), 1 - 66.","Wu, C. F. (1938) The stoneflies of China (Order Plecoptera). Bulletin of the Peking Society of Natural History, 13 (1), 53 - 87.","Wu, C. F. (1973) New species of Chinese stoneflies (order Plecoptera). Acta Entomologica Sinica, 16 (2), 97 - 118.","Claassen, P. W. (1940) A catalogue of the Plecoptera of the world. Agricultural Experiment Station Memoir 232. Cornell University Press, Ithaca, New York, 235 pp.","Ricker, W. E. (1952) Systematic studies on Plecoptera. Indiana University, Bloomington, Indiana, 200 pp.","Illies, J. (1966) Katalog der rezenten Plecoptera. Das Tierreich, Berlin, 82, 1 - 632.","Li, W. H. & Muranyi, D. (2015) A remarkable new genus of Perlodinae (Plecoptera: Perlodidae) from China, with remarks on the Asian distribution of Perlodinae and questions about its tribal concept. Zoologischer Anzeiger, 259, 41 - 53. https: // doi. org / 10.1016 / j. jcz. 2015.10.003","Yang, D. & Li, W. H. (2018) Species Catalogue of China. Vol. 2. Animals, Insecta (III), Plecoptera. Science Press, Beijing, 71 pp."]}

After reexamining the syntype series of Perlodinella kozlovi Klapálek, 1912, a new species of Perlodinella, P. tibetensis Huo & Du sp. nov. is proposed, and a lectotype is designated for P. kozlovi. Another monotypic genus of Perlodidae, Rauserodes Zwick (1999), is considered as a synonym of Perlodinella in this paper.

Genus Tibetisoperla Huo & Du, 2021 Tibetisoperla Huo & Du, 2021: 343. Type species T. wangluyui Huo & Du, 2021. Diagnosis Small sized. Macropterous. General color brown or black. Triocellate, anterior ocellus smaller. Tergum 10 with bilobate tip, covered with several large apical setae; paraprocts with spiny apical sclerite. Aedeagus membranous, covered by fine spines., Published as part of Yan, Yan-Hua, Chen, Zhen-Ning & Bozdoğan, Hakan, 2022, The species of Tibetisoperla (Plecoptera: Perlodidae), pp. 125-140 in European Journal of Taxonomy 823 on page 126, DOI: 10.5852/ejt.2022.823.1821, http://zenodo.org/record/6658611, {"references":["Huo Q. B. & Du Y. Z. 2021. A new genus of Isoperline (Plecoptera: Perlodidae) from Tibet, China. Zootaxa 4996 (2): 343 - 352. https: // doi. org / 10.11646 / zootaxa. 4996.2.8"]}

Tibetisoperla wangluyui Huo & Du, 2021 Fig 10 Tibetisoperla wangluyui Huo & Du, 2021: 343, figs 1–10 (original description). Material examined CHINA • 2 ♂♂; Qinghai Province, Tianjun County, Buha River; 37°34′8.51″ N, 98°39′24.07″ E; alt. 3535 m; 13–14 Jul. 2021; Wei-Hai Li leg.; HIST. Distribution China: Tibet and Qinghai provinces. Two males represent the first record for Qinghai Province. Remarks This dark brown species is characterized by reduced vesicle with semicircular posterior margin and membranous aedeagus lacking sclerite. Two males examined here represent the first record for Qinghai Province., Published as part of Yan, Yan-Hua, Chen, Zhen-Ning & Bozdoğan, Hakan, 2022, The species of Tibetisoperla (Plecoptera: Perlodidae), pp. 125-140 in European Journal of Taxonomy 823 on pages 138-139, DOI: 10.5852/ejt.2022.823.1821, http://zenodo.org/record/6658611, {"references":["Huo Q. B. & Du Y. Z. 2021. A new genus of Isoperline (Plecoptera: Perlodidae) from Tibet, China. Zootaxa 4996 (2): 343 - 352. https: // doi. org / 10.11646 / zootaxa. 4996.2.8"]}

Tibetisoperla elongata sp. nov. urn:lsid:zoobank.org:act: 7B5313C1-F26C-41C5-88BD-7B0486372A08 Figs 1–4 Diagnosis General color brownish. Vesicle developed and tongue-shaped, which is almost as long as its width. Aedeagus mostly membranous, except for a v-shaped ventrobasal sclerite, generally tubular and slightly curved ventrally at apex. Subgenital plate in female produced and subquadrate, posteriorly with a broad arcuate posteromedial notch. Etymology The specific epithet refers to the vesicle being long, which was presumed to be atypical for this genus. Material examined Holotype CHINA • ♂; Qinghai Province, Menyuan County, Daitong River; 37°21′37.99″ N, 101°36′55.30″ E; alt. 2782 m; 30 Jun. 2021; Wei-Hai Li and Rong-Rong Shen leg.; HIST. Paratypes CHINA • 1 ♂; same collection data as for holotype; HIST • 3 ♀♀; same collection data as for holotype; HIST • 1 ♂; same location as for holotype; 37°18′1.38″ N, 101°55′49.82″ E; alt. 2643 m; 26 Jul. 2021; Wei-Hai Li and Bin Zhang leg.; HIST. Description Male ADULT HABITUS (Figs 1A, 1C). Forewing length 8.1–8.5 mm, hindwing length 7.0– 7.2 mm (N=3). Head brown with dark brown arween ocelli and tentorial callosities. M-line dark brown. Triocellate, anterior ocellus smaller. Antennae brownish, scape brown, palpi brownish. Pronotum disc with large area of brown to dark brown rugosities and pale medial stripe. Legs brownish. Wings hyaline, veins brown. ABDOMEN (Figs 1D, 2). Abdominal terga brownish and terga 1–4 darkly sclerotized. Terga 2–9 each with a pair of small dark spots medially and dark medial strip (obscure in young individual). Tergum 9 bearing two paramedial patches of sensilla on darker posterior margin, patches separated by pale T-shaped mesal membrane. Tergum 10 bilobate, distally upcurved with 3–4 thick apical setae. Vesicle located on posterior margin of sternum 8, generally tongue-shaped, ca almost as long as its width, basally brown but apex paler, covered with hairs. Paraprocts sclerotized, with partially separated apical sclerite covered by small terminal spines. AEDEAGUS (Fig. 3). Membranous except for a v-shaped ventrobasal sclerite, tubular and slightly curved ventrally at apex, mostly covered by fine spinules, dorsoapical and ventroapical portions bald. Female ADULT HABITUS (Fig. 1B). Forewing length 9.0– 9.9 mm, hindwing length 7.9–8.2 mm. Body coloration similar to male. ABDOMEN (Fig. 4). Tergum 10 unmodified. Subgenital plate sclerotized and subquadrate, produced posteriorly covering anterior half of sternum 9, with a broad arcuate posteromedial notch. EGG. Unknown. Distribution China: Qinghai Province. Presently only known from two sites along the riverside of Daitong River in Menyuan County. Remarks The type species of Tibetisoperla, T. wangluyui Huo & Du, 2021 is a dark species and has a reduced vesicle on sternum 8, but the new species is a brownish species and has a developed vesicle which is present in typical Isoperla Banks, 1906 (Teslenko & Zhiltzova 2009; Murányi 2011; Szczytko & Kondratieff 2015; Cao et al. 2020), another genus of Isoperlinae in China. However, the large apical setae of tergum 10 and spiny apical sclerite of paraproct clearly show its placement in Tibetisoperla. The female of T. elongata is different to T. wangluyui in subgenital plate structure, the former is broadly semicircular while the latter is subquadrate. Additionally, T. elongata with a typically posterior notch which is indistinct of T. sclerotica. Terga 1–8 of female with black medial stripes and subgenital plate is broadly triangular in T. sclerotica., Published as part of Yan, Yan-Hua, Chen, Zhen-Ning & Bozdoğan, Hakan, 2022, The species of Tibetisoperla (Plecoptera: Perlodidae), pp. 125-140 in European Journal of Taxonomy 823 on pages 126-130, DOI: 10.5852/ejt.2022.823.1821, http://zenodo.org/record/6658611, {"references":["Huo Q. B. & Du Y. Z. 2021. A new genus of Isoperline (Plecoptera: Perlodidae) from Tibet, China. Zootaxa 4996 (2): 343 - 352. https: // doi. org / 10.11646 / zootaxa. 4996.2.8","Banks N. 1906. On the perlid genus Chloroperla. Entomological News 17: 174 - 175.","Teslenko V. A. & Zhiltzova L. A. 2009. Key to the Stoneflies (Insecta, Plecoptera) of Russia and Adjacent Countries. Imagines and Nymphs. Russian Academy of Sciences, Far Eastern Branch, Dalnauka, Vladivostok.","Muranyi D. 2011. Balkanian species of the genus Isoperla Banks, 1906 (Plecoptera: Perlodidae). Zootaxa 3049: 1 - 46. https: // doi. org / 10.1643 / CE- 08 - 219","Szczytko S. W. & Kondratieff B. C. 2015. A Review of the Eastern Nearctic Isoperlinae (Plecoptera: Perlodidae) with the description of twenty-two new species. Monographs of Illiesia 1: 1 - 289.","Cao Z. S., Wang Y. & Li W. H. 2020. A new species of Isoperla (Plecoptera: Perlodidae) from China. Zootaxa 4858 (2): 251 - 260. https: // doi. org / 10.11646 / zootaxa. 4858.2.6"]}

Yan, Yan-Hua, Chen, Zhen-Ning, Bozdoğan, Hakan (2022): The species of Tibetisoperla (Plecoptera: Perlodidae). European Journal of Taxonomy 823: 125-140, DOI: https://doi.org/10.5852/ejt.2022.823.1821, URL: http://zoobank.org/fbfd2e52-c902-4f61-9eb4-a8badf997f0b

Tibetisoperla wangluyui Huo & Du, 2021 Fig 10 Tibetisoperla wangluyui Huo & Du, 2021: 343, figs 1–10 (original description). Material examined CHINA • 2 ♂♂; Qinghai Province, Tianjun County, Buha River; 37°34′8.51″ N, 98°39′24.07″ E; alt. 3535 m; 13–14 Jul. 2021; Wei-Hai Li leg.; HIST. Distribution China: Tibet and Qinghai provinces. Two males represent the first record for Qinghai Province. Remarks This dark brown species is characterized by reduced vesicle with semicircular posterior margin and membranous aedeagus lacking sclerite. Two males examined here represent the first record for Qinghai Province.

Genus Tibetisoperla Huo & Du, 2021 Tibetisoperla Huo & Du, 2021: 343. Type species T. wangluyui Huo & Du, 2021. Diagnosis Small sized. Macropterous. General color brown or black. Triocellate, anterior ocellus smaller. Tergum 10 with bilobate tip, covered with several large apical setae; paraprocts with spiny apical sclerite. Aedeagus membranous, covered by fine spines.

Tibetisoperla sclerotica sp. nov. urn:lsid:zoobank.org:act: 5F951431-4255-4A09-B0BB-A645800BB1B1 Figs 5–9 Diagnosis General color dark brown. Vesicle reduced to a black transverse band. Ventrobasal sclerite of aedeagus black, generally subquadrate in outline with a triangular postcentral notch. Etymology The specific epithet refers to the sclerotized ventrobasal sclerite of everted aedeagus. Material examined Holotype CHINA • ♂; Qinghai Province, Menyuan County, Meihua village; 37°16′6.99″ N, 102°8′3.58″ E; alt. 2979 m; 30 Jul. 2021; Wei-Hai Li leg.; HIST. Paratypes CHINA – Qinghai Province • 1 ♀; same locality as for holotype; 31 Jul. 2021; HIST • 2 ♀♀; Menyuan County, Xiami Town, Qihankaigou; 37°9′30.73″ N, 102°1′52.09″ E; alt. 2694 m; 3 Jul. 2021; Wei-Hai Li and Rong-Rong Shen leg.; HIST • 1 ♀; Menyuan County, Zhugu Village, Sigou; 37°7′54.39″ N, 102°23′57.56″ E; alt. 2570 m; 28 Jul. 2021; Bin Zhang leg.; HIST • 1 ♀; Menyuan County, Liuhuanggou; 37°47′45.24″ N, 101°14′53.16″ E; alt. 3407.8 m; 29 Jul. 2021; Zhuo-Yin Jiang leg.; HIST • 2 ♀♀; Qilian County, Zhamashenxigou, Dongchagou; 38°9′14.18″ N, 100°1′22.40″ E; alt. 3015 m; 9 Jul. 2021; Wei Zeng leg.; CAU. Description Male ADULT HABITUS (Figs 5A, C–D). General color dark brown to black (Fig. 5A). Head mostly brown, with dark area around ocelli, tentorial callosities and M-line, the area sometimes extending to center of occiput. M-line dark brown. Triocellate, anterior ocellus slightly smaller. Antennae brown, scape darker, palpi brownish. Pronotum disc with dark brown rugosities and wide, brown, longititudinal stripe disjunct with thin black midline. Legs brown, femora darker. Wings hyaline, veins brown. Cerci brownish, apical segments darker. ABDOMEN (Figs 5D, 6, 7A–B). Forewing length ca 7.1 mm, hindwing length ca 6.0 mm. Abdominal tergal pattern obscure due to entirely black coloration. Tergum 9 bearing two patches of black sensilla on posterior margin by triangular medial membranous area extended anterolaterally forming a T-shaped paler marking. Tergum 10 with thin, longitudinal, pale, medial line; distal half bilobate, with 4–5 apical setae. Vesicle located on posterior margin of sternum 8, reduced to black transverse band. Paraprocts with recurved spiny apical sclerite, covered by ca. 13–14 tiny spines. AEDEAGUS (Fig. 8). Mostly membranous except for a dark ventrobasal sclerite, sclerite subquadrate with triangular postcentral notch; greatly enlarged posterior to ventrobasal sclerite. Aedeagus apical third upcurved and generally globular before narrowest nipple-like apex, brownish; mostly covered by fine spinules but lacking large spine. Female ADULT HABITUS (Figs 5B, 9A). Forewing length 7.8–9.8 mm, hindwing length 5.8–8.0 mm (N=7). Body coloration generally similar to male. ABDOMEN (Fig. 9B–D). Abdominal segments lighter and terga 1–8 with black medial tergal stripes in young individuals. Tergum 10 unmodified. Sternum 8 with sclerotized lateral portion and posterior subgenital plate, anteromedial portion membranous. Subgenital plate broadly triangular with dense long hairs, covering anterior third of sternum 9, with a posteromedial notch; lateral margins partially separated and heavily sclerotized. Sternum 9 with a pair of slope paramedial strips. EGG. Unknown. Distribution China: Qinghai Province. Presently only known from Menyuan County. Remarks The new species closely resembles T. wangluyui Huo & Du, 2021 in dark body coloration and reduced vesicle on sternum 8, but it is easily separated from that species by the large ventrobasal sclerite of aedeagus which is entirely membranous in T. wangluyui.

Tibetisoperla sclerotica sp. nov. urn:lsid:zoobank.org:act: 5F951431-4255-4A09-B0BB-A645800BB1B1 Figs 5–9 Diagnosis General color dark brown. Vesicle reduced to a black transverse band. Ventrobasal sclerite of aedeagus black, generally subquadrate in outline with a triangular postcentral notch. Etymology The specific epithet refers to the sclerotized ventrobasal sclerite of everted aedeagus. Material examined Holotype CHINA • ♂; Qinghai Province, Menyuan County, Meihua village; 37°16′6.99″ N, 102°8′3.58″ E; alt. 2979 m; 30 Jul. 2021; Wei-Hai Li leg.; HIST. Paratypes CHINA – Qinghai Province • 1 ♀; same locality as for holotype; 31 Jul. 2021; HIST • 2 ♀♀; Menyuan County, Xiami Town, Qihankaigou; 37°9′30.73″ N, 102°1′52.09″ E; alt. 2694 m; 3 Jul. 2021; Wei-Hai Li and Rong-Rong Shen leg.; HIST • 1 ♀; Menyuan County, Zhugu Village, Sigou; 37°7′54.39″ N, 102°23′57.56″ E; alt. 2570 m; 28 Jul. 2021; Bin Zhang leg.; HIST • 1 ♀; Menyuan County, Liuhuanggou; 37°47′45.24″ N, 101°14′53.16″ E; alt. 3407.8 m; 29 Jul. 2021; Zhuo-Yin Jiang leg.; HIST • 2 ♀♀; Qilian County, Zhamashenxigou, Dongchagou; 38°9′14.18″ N, 100°1′22.40″ E; alt. 3015 m; 9 Jul. 2021; Wei Zeng leg.; CAU. Description Male ADULT HABITUS (Figs 5A, C–D). General color dark brown to black (Fig. 5A). Head mostly brown, with dark area around ocelli, tentorial callosities and M-line, the area sometimes extending to center of occiput. M-line dark brown. Triocellate, anterior ocellus slightly smaller. Antennae brown, scape darker, palpi brownish. Pronotum disc with dark brown rugosities and wide, brown, longititudinal stripe disjunct with thin black midline. Legs brown, femora darker. Wings hyaline, veins brown. Cerci brownish, apical segments darker. ABDOMEN (Figs 5D, 6, 7A–B). Forewing length ca 7.1 mm, hindwing length ca 6.0 mm. Abdominal tergal pattern obscure due to entirely black coloration. Tergum 9 bearing two patches of black sensilla on posterior margin by triangular medial membranous area extended anterolaterally forming a T-shaped paler marking. Tergum 10 with thin, longitudinal, pale, medial line; distal half bilobate, with 4–5 apical setae. Vesicle located on posterior margin of sternum 8, reduced to black transverse band. Paraprocts with recurved spiny apical sclerite, covered by ca. 13–14 tiny spines. AEDEAGUS (Fig. 8). Mostly membranous except for a dark ventrobasal sclerite, sclerite subquadrate with triangular postcentral notch; greatly enlarged posterior to ventrobasal sclerite. Aedeagus apical third upcurved and generally globular before narrowest nipple-like apex, brownish; mostly covered by fine spinules but lacking large spine. Female ADULT HABITUS (Figs 5B, 9A). Forewing length 7.8–9.8 mm, hindwing length 5.8–8.0 mm (N=7). Body coloration generally similar to male. ABDOMEN (Fig. 9B–D). Abdominal segments lighter and terga 1–8 with black medial tergal stripes in young individuals. Tergum 10 unmodified. Sternum 8 with sclerotized lateral portion and posterior subgenital plate, anteromedial portion membranous. Subgenital plate broadly triangular with dense long hairs, covering anterior third of sternum 9, with a posteromedial notch; lateral margins partially separated and heavily sclerotized. Sternum 9 with a pair of slope paramedial strips. EGG. Unknown. Distribution China: Qinghai Province. Presently only known from Menyuan County. Remarks The new species closely resembles T. wangluyui Huo & Du, 2021 in dark body coloration and reduced vesicle on sternum 8, but it is easily separated from that species by the large ventrobasal sclerite of aedeagus which is entirely membranous in T. wangluyui., Published as part of Yan, Yan-Hua, Chen, Zhen-Ning & Bozdoğan, Hakan, 2022, The species of Tibetisoperla (Plecoptera: Perlodidae), pp. 125-140 in European Journal of Taxonomy 823 on pages 132-135, DOI: 10.5852/ejt.2022.823.1821, http://zenodo.org/record/6658611, {"references":["Huo Q. B. & Du Y. Z. 2021. A new genus of Isoperline (Plecoptera: Perlodidae) from Tibet, China. Zootaxa 4996 (2): 343 - 352. https: // doi. org / 10.11646 / zootaxa. 4996.2.8"]}

Tibetisoperla elongata sp. nov. urn:lsid:zoobank.org:act: 7B5313C1-F26C-41C5-88BD-7B0486372A08 Figs 1–4 Diagnosis General color brownish. Vesicle developed and tongue-shaped, which is almost as long as its width. Aedeagus mostly membranous, except for a v-shaped ventrobasal sclerite, generally tubular and slightly curved ventrally at apex. Subgenital plate in female produced and subquadrate, posteriorly with a broad arcuate posteromedial notch. Etymology The specific epithet refers to the vesicle being long, which was presumed to be atypical for this genus. Material examined Holotype CHINA • ♂; Qinghai Province, Menyuan County, Daitong River; 37°21′37.99″ N, 101°36′55.30″ E; alt. 2782 m; 30 Jun. 2021; Wei-Hai Li and Rong-Rong Shen leg.; HIST. Paratypes CHINA • 1 ♂; same collection data as for holotype; HIST • 3 ♀♀; same collection data as for holotype; HIST • 1 ♂; same location as for holotype; 37°18′1.38″ N, 101°55′49.82″ E; alt. 2643 m; 26 Jul. 2021; Wei-Hai Li and Bin Zhang leg.; HIST. Description Male ADULT HABITUS (Figs 1A, 1C). Forewing length 8.1–8.5 mm, hindwing length 7.0– 7.2 mm (N=3). Head brown with dark brown arween ocelli and tentorial callosities. M-line dark brown. Triocellate, anterior ocellus smaller. Antennae brownish, scape brown, palpi brownish. Pronotum disc with large area of brown to dark brown rugosities and pale medial stripe. Legs brownish. Wings hyaline, veins brown. ABDOMEN (Figs 1D, 2). Abdominal terga brownish and terga 1–4 darkly sclerotized. Terga 2–9 each with a pair of small dark spots medially and dark medial strip (obscure in young individual). Tergum 9 bearing two paramedial patches of sensilla on darker posterior margin, patches separated by pale T-shaped mesal membrane. Tergum 10 bilobate, distally upcurved with 3–4 thick apical setae. Vesicle located on posterior margin of sternum 8, generally tongue-shaped, ca almost as long as its width, basally brown but apex paler, covered with hairs. Paraprocts sclerotized, with partially separated apical sclerite covered by small terminal spines. AEDEAGUS (Fig. 3). Membranous except for a v-shaped ventrobasal sclerite, tubular and slightly curved ventrally at apex, mostly covered by fine spinules, dorsoapical and ventroapical portions bald. Female ADULT HABITUS (Fig. 1B). Forewing length 9.0– 9.9 mm, hindwing length 7.9–8.2 mm. Body coloration similar to male. ABDOMEN (Fig. 4). Tergum 10 unmodified. Subgenital plate sclerotized and subquadrate, produced posteriorly covering anterior half of sternum 9, with a broad arcuate posteromedial notch. EGG. Unknown. Distribution China: Qinghai Province. Presently only known from two sites along the riverside of Daitong River in Menyuan County. Remarks The type species of Tibetisoperla, T. wangluyui Huo & Du, 2021 is a dark species and has a reduced vesicle on sternum 8, but the new species is a brownish species and has a developed vesicle which is present in typical Isoperla Banks, 1906 (Teslenko & Zhiltzova 2009; Murányi 2011; Szczytko & Kondratieff 2015; Cao et al. 2020), another genus of Isoperlinae in China. However, the large apical setae of tergum 10 and spiny apical sclerite of paraproct clearly show its placement in Tibetisoperla. The female of T. elongata is different to T. wangluyui in subgenital plate structure, the former is broadly semicircular while the latter is subquadrate. Additionally, T. elongata with a typically posterior notch which is indistinct of T. sclerotica. Terga 1–8 of female with black medial stripes and subgenital plate is broadly triangular in T. sclerotica.

Isoperla nagyi sp. n. is described on the basis of morphology of male and female adults and eggs. The new species is classified as an isolated species within the West Palaearctic Isoperla. It was found in the lower elevations of the Ţarcu Mts that is forming a high range of the westernmost Southern Carpathians. Further contributions are given on the stonefly fauna of the Ţarcu Mts, including notes on the Romanian distribution of Brachyptera bulgarica Raušer, and the taxonomy and distribution of Isoperla pusilla (Klapálek).

In this study, we analysed the diet of two populations of Perlodes intricatus (Pictet, 1841) inhabiting river sections characterised by different environmental quality. Macrobenthic communities and...

Materials on the aquatic invertebrate fauna in the Maly Patok River basin (the western slopes of the Subpolar Urals) have been presented. These water bodies, which maintain the natural hydrological regime, are located on the territory of the Yugyd-Va National Park. A total of 108 taxa (except for Chironomidae) in the zoobenthos samples and the invertebrate qualitative collections taken in the summer periods in 1996, 2000–2007, and 2017 are identified. Among them, amphibiotic insects prevail in the watercourses, while oligochaetes and mollusks dominate in the lakes in regards to number of species. Mayflies of the families Baetidae and Heptageniidae, stoneflies of Perlodidae, caddisflies of Rhyacophilidae and Apataniidae, and the Elmidae beetles are mostly present in the rivers. Oligochaetes of Naididae and mollusks of the families Planorbidae and Sphaeriidae are most abundant and diverse in the lakes. The most frequent species have Palearctic (29.0%) and Holarctic (25.5%) habitat ranges. In addition to the widespread European species, some species are recorded that have the largest part of their ranges in Siberia. Rare species in the fauna composition have been revealed.

The larva of the Balkan microendemic Isoperla vevcianensis Ikonomov, 1980 (Plecoptera: Perlodidae: Isoperlinae) is described on the basis of larvae associated with adults by means of cox1 sequences similarities. Eggs and everted penis of the male are described for the first time. The larva possessed blunt paraprocts which were previously sharp among Palaearctic larvae of this huge Holarctic and Oriental genus. The commonly pointed paraproct remains as the only distinguishing generic character for the morphologically diverse larvae of Palaearctic Isoperla, but should not be considered as an exclusive character state for the genus. Morphological characters are illustrated in comparison with the sympatric larvae of the Isoperla tripartita species complex.

Isoperla vevcianensis Ikonomov, 1980 (Figs. 1���27) Isoperla vevcianensis Ikonomov, 1980 ��� Ikonomov 1980: 26. (original description of the male, female and larva); Mur��nyi 2011: 21. (redescription of the male). Type locality: North Macedonia, small streams above Vevchani, at elevations 1100, 1300 and 1450 m (Jablanica Mts). N ew records: NORTH MACEDONIA: Southwestern region, Jablanica Mts, Labuni��ta, open brook W of the city, 1905 m, N 41�� 16.069��� E 20�� 31.242���, 26.vi.2014, leg. P��ter Juh��sz, Tibor Kov��cs, D��vid Mur��nyi: 5♂ 1♀, 2 larvae (HNHM: PLP 4840), 7♂ 5♀, 1 larva (MM). Diagnosis. Both adult and larva with large head. Male medial penial armature with upper part trapezoid, lower part rounded to rounded triangular, lateral armatures lacking. Egg oval, with marked ornamentation of FCIs, collar with slightly flanged rim. Larva with pronotum half as long as wide, lacinia trapezoid shaped, paraproct blunt with widely rounded apex, cercus lacks dorsal hair fringe. Description of the larva: Body length of the mature larva: 16.0���18.0 mm (n=3). General colour brown, with indistinct paler markings on the dorsum and pale ventral aspect (Figs. 1, 3���4). Pilosity usual for the genus, consists of fine black clothing hairs and acute marginal spines. Occipital row dense, medially interrupted only at the occipital suture (Figs. 1, 9���10); pronotal marginal fringe distinct, longest spines are as long as one tenth of pronotal width (Figs. 1, 12); posterior tergal fringe with acute spines nearly half as long as tergal length on tergum 5 (Figs. 3, 13); cercal apical fringes dense but short and acute, on medial cercomeres most are shorter than one sixth of the length of segments but one or two spines of the fringe reach about one third of the length (Fig. 14); dorsal hair fringe lacking from the entire cercus (Figs. 7, 14). Head mostly brown and lacks interocellar spot, yellow markings limited to a small spot on the occipital suture, tentorial callosities and M-line; occipital rugosities and lateral occipital spots obscure; eyes small, the head is rather wide and frontal area slightly elongated (Fig. 1). Scape and apical half of pedicel brown, following antennomeres paler; palpi and mouthparts light brown, with dark edges on the maxilla and mandible (Fig. 11). Lacinia bidentate, wide trapezoid shaped; subapical tooth more than half of the length of apical tooth; submarginal row with one A seta interrupted from three B setae, the last one is being much shorter than previous two; one thin marginal seta (TMS) and one dorsal seta (DS) present; 9���11 marginal setae (C) initially widely spaced, last few shorter and closer, difficult to differentiate from dorsal surface setae; 12���16 ventral surface setae (D) starting from submarginal setae B, ending posteriorly at approximately four fifth of the inner lacinia margin length; dorsal surface setae (DSS) continue from last marginal seta (C) as a single submarginal row along inner-lateral margin, ending before posteriormost ventral surface setae (Figs. 15, 17, 19). Galea with 6���10 surface setae and 3���4 apical setae, basally slightly swollen, reaching base of apical tooth (Figs. 15, 17, 19). Mandible with three molars and three incisor dens, molar brush dense and long (Figs. 16, 18, 20). Pronotum short and slightly narrower than head, length half of width; brown with indistinct paler rugosities (Fig. 1). Mesonotum and metanotum mostly brown with indistinct pale brown pattern, wingpads paler; outer margin of wingpads nearly straight (Fig. 1). Thoracic sternites entirely pale, furcasternites and furcal pits large and slightly darker (Fig. 4). Legs entirely brown, tarsal claw lengths equal (Figs. 1, 3). Abdomen divided laterally by pleura in the first two segments; abdominal terga brown with moderately distinct, paired oval pale patch medially (Figs. 3, 13). Ventral surface of abdomen pale, the distal segments slightly darker. Paraprocts brown, large and blunt, apex widely rounded (Figs. 5, 7). Cercus light brown to brown, cercomeres cylindrical; medial cercomeres twice as long as wide (Fig. 14). Updated characters of the male: Paraprocts dark brown, blunt, wide and slightly recurved in dorsal view (Fig. 63 in Mur��nyi (2011)). In caudal view, the base is wide and bears only a very small membranous portion; apex bluntly triangular, inner edge slightly sinuous (Fig. 22). Penis (Fig. 21): Divided into four lobes and a tubular basal section when extruded, lateral lobes poorly developed. The medial penial armature is divided into an upper and a lower part, lateral penial armatures lacking. Lower part of the medial armature located on the ventral lobe, upper part is on the medial lobe between the vestigial lateral lobes. The upper part of medial armature trapezoidal, tapered towards basal section; length 350 ��m, width 200 ��m. The lower part of medial armature is rounded triangular or rounded, length 200 ��m, width 220 ��m. The scales are spike-like, all of the same width, but those on the lower part are less dense, and generally shorter; width 15 ��m, length 30���100 ��m. The ventral lobe is small and weakly separated from the basal section, mostly aspinulate besides bearing the lower part of medial armature; spike-like coloured scales of the armature gradually turn into colourless triangular scales basolaterally, triangular scales continued on the basal section. The medial lobe is large and spherical, bearing the upper part of medial armature; upper edge of the armature transitioning into colourless triangular scales, then rapidly transitioning into hydra-like scales that are covering a wide area on the ventrobasal surface, becoming denser towards margins of the scale field; apical area aspinulate, lateral margin connecting small lateral lobes with small hydra-like scales. Lateral lobe is poorly developed and hardly delimited from medial lobe; mostly smooth, but with lateral band of small hydra-like scales, and a few sensillae. The basal section is completely covered by triangular scales. Egg: Chorion brown; length 0.28���0.35 mm, width 0.22���0.26 mm (n=8). Shape oval, opercular end usually depressed, collar end less rounded; cross section rounded or indented. Hatching line inconspicuous. Micropyles in a row on the opercular third, not raised, each located on the junction of carinae between follicular cell impressions (FCIs). Chorion with marked ornamentation of penta- and hexagonal FCIs. Collar round, rim slightly flanged, bears one row of FCIs. Anchor large and flat. Distribution and biology: Isoperla vevcianensis has a narrow endemic distribution, restricted to the Jablanica Mts, on the border zone of Albania and North Macedonia (Ikonomov 1980, Mur��nyi 2011). The species is mostly associated with headwater brooks in the alpine and subalpine grasslands, at elevations above 1100 meters (Figs. 25���27). The adults emerge in June and July. The adult and larval specimens from our study were found together with Leuctra metsovonica Aubert, 1956, Perla cf. pallida and another Isoperla species belonging to the I. tripartita species complex (sensu Mur��nyi et al. 2016). Species relationships: Isoperla vevcianensis was assigned to the Isoperla silesica group sensu Mur��nyi (2011) on the basis of the male penial armatures. Monophyly of the two Central European-Alpine species, I. silesica Illies, 1952 and I. zwicki Tierno de Figueroa & Fochetti, 2001 with the Central Balkanian I. vevcianensis and I. breviptera Ikonomov, 1980 should be confirmed by details of the penial lobes and cox-1 sequences, but these are known only for I. vevcianensis. The mature larva is distinctive among congeners by its large head and short pronotum, similar to the Alpine I. claudiae Graf & Konar, 2014 (in: Graf et al. 2014) and the Crimean I. prokopovi Zhiltzova & Zwick, 2012. An undescribed species from Montenegro, closely related to I. breviptera, has similar large head and short pronotum (W. Graf, pers. comm.). In the collection of the MM, we have larvae similar to the Montenegrian species, and probably belong to the same undescribed species from Kosovo. The wide trapezoid shaped lacinia is also unique among European species, but this character is rather variable among Nearctic species (Hilsenhoff & Billmyer 1973, Stewart & Stark 2002, Sandberg 2011). The blunt paraproct is unique among the known larvae of the genus (Zwick 2004), though this character is not documented for the East Palaearctic and Nearctic species (Stewart & Stark 2002, Teslenko & Zhiltzova 2006, and Sandberg 2011). For comparison, head, pronotum and paraprocts of sympatric larvae of the unidentified member of the I. tripartita species complex are depicted on Figs. 2, 6, and 8. Larvae of the I. tripartita species complex display characters typical for the West Palaearctic Isoperla. The egg of I. vevcianensis proved to be the usual type of West Palaearctic species, similar to those of e.g. I. tripartita tripartita as depicted in Mur��nyi (2011). Genetics. Haplotypes from Isoperla vevcianensis formed a cluster of identical sequences for the coxI gene (Fig. 24) with zero intraspecific genetic distance. Molecular analyses agreed with the morphological association between female, male and larvae of I. vevcianensis based on sequence similarities among the specimens. Interspecific genetic distances between I. vevcianensis and the members of the I. tripartita species complex exhibited a 3% divergence, suggesting different species., Published as part of Mur��nyi, D��vid, Kov��cs, Tibor, Gamboa, Maribet & Watanabe, Kozo, 2021, Loss of a larval generic character: an interesting and new description for Isoperla vevcianensis Ikonomov, 1980 (Plecoptera: Perlodidae) with updated adult characters, pp. 541-552 in Zootaxa 5082 (6) on pages 543-548, DOI: 10.11646/zootaxa.5082.6.2, http://zenodo.org/record/5797606, {"references":["Ikonomov, P. (1980) Nouvelles especes de Plecopteres (Insecta, Plecoptera) de Macedoine. II. Fragmenta Balcanica, 11 (4), 19 - 31.","Muranyi, D. (2011) Balkanian species of the genus Isoperla Banks, 1906 (Plecoptera: Perlodidae). Zootaxa, 3049, 1 - 46.","Aubert, J. (1956) Contribution a l'etude des Plecopteres de Grece. Mitteilungen der Schweizerischen Entomologischen Gesellschaft, 29 (2), 187 - 213.","Muranyi, D., Kovacs, T. & Orci, K. M. (2016) Contribution to the taxonomy and biology of two Balkan endemic Isoperla Banks, 1906 (Plecoptera: Perlodidae) species. Zoosymposia, 11, 73 - 88. https: // doi. org / 10.11646 / zoosymposia. 11.1.11","Illies, J. (1952) Zwei neue arten der Plecopteren gattung Isoperla aus dem deutschen Mittelgebirge. Zoologischer Anzeiger, 149, 42 - 48.","Tierno de Figueroa, J. M. & Fochetti, R. (2001) Isoperla zwicki sp. n. (Plecoptera, Perlodidae) a new Italian stonefly species. Revue suisse de Zoologie, 108 (3), 483 - 486. https: // doi. org / 10.5962 / bhl. part. 80157","Graf, W., Konar, M., Muranyi, D., Orci, K. M. & Vitecek, S. (2014) A new species of Isoperla (Insecta, Plecoptera) from the Karawanken, with considerations on the Southern Limestone Alps as centers of Endemism. ZooKeys, 448, 27 - 36. https: // doi. org / 10.3897 / zookeys. 448.8509","Zhiltzova, L. A. & Zwick, P. (2012) Isoperla prokopovi, a new presumedly parthenogenetic stonefly from Crimea (Plecoptera, Perlodidae). Illiesia, 8 (4), 37 - 44.","Hilsenhoff, W. L. & Billmyer, S. J. (1973) Perlodidae (Plecoptera) of Wisconsin. The Great Lakes Entomologist, 6 (1), 1 - 14.","Stewart, K. W. & Stark, B. P. (2002) Nymphs of North American stonefly genera (Plecoptera). 2 nd Edition. The Caddish Press, Columbus, Ohio, 510 pp.","Sandberg, J. B. (2011) The Isoperla of California (Plecoptera: Perlodidae); larval descriptions and a key to 17 Western Nearctic species. Illiesia, 7 (22), 202 - 258.","Zwick, P. (2004) A key to the West Palaearctic genera of stoneflies (Plecoptera) in the larval stage. Limnologica, 34, 315 - 348. https: // doi. org / 10.1016 / S 0075 - 9511 (04) 80004 - 5","Teslenko, V. A. & Zhiltzova, L. A. (2006) Nymphs of the genus Isoperla Banks (Plecoptera, Perlodidae) from the Eastern Palaearctic Region. Zootaxa, 1130 (1), 1 - 33. https: // doi. org / 10.11646 / zootaxa. 1130.1.1"]}

Currently, information on the biology of Plecoptera from China is scarce, particularly on mating behavior. In this paper, the existence of mating mistakes (erroneous mating attempts) involving 13 Chinese stonefly species (belonging to nine genera and three families) is reported. These erroneous mating behaviors can be included into three different categories: mating attempts between conspecific males (including the formation of erroneous mating balls), mating attempts between different taxa (including displacement attempts during copulation), and mating-related behaviors with non-living objects. From these behaviors, some aspects of stoneflies during mating, such as the physical competition between males, the sensorial mechanisms implied in triggering a mating behavior, the conditions favoring the mating mistakes, and the possible consequences of interspecific mating in the hybrid production, are discussed., National Natural Science Foundation of China (No. 32170459; 31872266; 31071958), China Scholarship Council, the Project of Biological Resources Survey in Wuyishan National Park, Investigation and Evaluation of Insect Resources in Huangshan Scenic Spot (Phase I), Insect fauna of Qilian Mountain National Park (Qinghai), Volume of Lepidoptera and Diptera (procurement contract No. QHTX-2021-006, Administration of Qilian Mountain Nature Reserve, Qinghai Province)

Isoperla chongxui sp. nov. Figs. 1���11. Adult habitus. Body pale to dark brown, males much darker than females (Figs. 1���4). Head mostly pale brown, medially covered by an elliptical, dark stigma. Triocellate, anterior ocellus slightly smaller than posterior ones; compound eyes small and rounded. Antennae slender, generally dark brown, basal segments pale, subequal in length to the abdomen. Maxillary palps slender, four-segmented with a strongly reduced apical segment reminiscent of Chloroperlidae; labial palps shorter, three-segmented with a strongly reduced apical segment. Pronotum subrectangular with angled corners; median half of pronotum rugose and dark brown, lateral areas pale. Macropterous; wings hyaline, veins brown. Legs generally pale brown; two large tibial spurs present. Abdominal segments mostly dark brown in males, pale in females. Cerci slender, shorter than the abdomen, mostly pale, apically dark brown. Male. Body length 8���10 mm (N = 5); forewings length ca. 8.0 mm, hindwings length ca. 6.5 mm (Figs. 1���2). In the forewing, RP with two branches; CuA with three to four branches; AA1 simple, AA2 forked. In the hind wing, RP with two branches; anal area large and folded with eight anal branches. Abdominal tergum 1 completely sclerotized. Terga 1���9 with membranous median areas (Fig. 5). Lateral areas of terga 5���6 slightly humped (Figs. 5���6). Terga 7���9 each with a pair of strongly elevated lateral lobes, the lobes gradually enlarged towards posterior segments (Figs. 5���6). Posteromedial margin of tergum 9 rounded and sclerotized. Tergum 10 mostly sclerotized except for the posteromedial membranous area (Fig. 5D). Paraprocts triangular, rising to height of 10th tergum, hook-shaped and curved anteriorly, apices pointed (Figs. 5D, G, 6A, B). Vesicle poorly developed,>2X wider than long, reduced into an elliptical patch of dark hairs on posteromedial margin of sternum 8 (Fig. 7). Sternum 9 broadly produced, expanded backwards with a truncate apex (Fig. 7A, B). Aedeagus membranous, basoventrally with an elliptical spinule patch, apically with a spinulose lobe connected with a longitudinal stripe of dense spines; lateral membranous lobes present on apex of aedeagus (Fig. 8). Female. Body length 12.5���13.5 mm (N = 5) (Figs. 3���4).Abdominal segments pale.Tergum 10 with subtriangular posterior margin (Fig. 9A). Subgenital plate broad, gradually tapered towards apex, covering anterior ⅓ of sternum 9, posterior margin with a shallow notch (Fig. 9B). Paraprocts subtriangular with pointed apices (Fig. 9). The immature eggs rounded in shape; collar short, cylindrical; anchor near rounded from view of the anchor pole, surface without granules; chorionic surface with hexagonal follicle cell impressions (Fig. 10). Type material. Holotype male, China: Henan Province, Xinyang City, Dongzhai National Nature Reserve (Fig. 11), 31.94956609�� N, 114.25485947�� E, 160 m, 6-IV-2021, Si-Kai, Du (ICJUST). Paratypes: four males and five females, same locality and date as holotype (ICJUST). Etymology. The species is named for Mr. Xu Chong who has helped the authors in previous works. Remarks. The new species can be easily distinguished from its congeners by the presence of projected lobes on male abdominal terga 7���9 and smaller humps on previous tergal segments. In I. sextuberculata, the finger-shaped lobes are present on terga 4, 7 and 8 (Huo & Du 2020). Other species of Isoperlinae with abdominal processes include Isoperla distincta Nelson, 1976 from North America, Kaszabia digitata (Kawai, 1963), Isoperla sp. (Maruyama & Hanada 2016) from Japan, and Kaszabia nigricauda (Nav��s, 1923) from Mongolia and Russia. Locations of tergal lobes on these species are on different segments than those found on the new species., Published as part of Chen, Zhi-Teng, Du, Si-Kai & Li, Xin-Tong, 2021, Description of a remarkable new species of Isoperla (Plecoptera: Perlodidae), with supplements for Isoperla kozlovi Zhiltzova, 1972 from China, pp. 160-174 in Zootaxa 5027 (2) on pages 161-164, DOI: 10.11646/zootaxa.5027.2.2, http://zenodo.org/record/5448186, {"references":["Huo, Q. B. & Du, Y. Z. (2020) A new remarkable Isoperla species with abdominal processes from Hubei Province of Central China (Plecoptera: Perlodidae). Zootaxa, 4801 (3), 552 - 558. https: // doi. org / 10.11646 / zootaxa. 4801.3.7","Nelson, C. H. (1976) A new species of Isoperla (Plecoptera: Perlodidae) from Tennessee. Journal of the Kansas Entomological Society, 49, 212 - 214.","Kawai, T. (1963) A new species of the genus Isoperla (Plecoptera) from Echigo, Japan. Kontyu, 31, 61 - 63.","Maruyama, H. & Hanada, S. (2016) A field guide to Japanese aquatic insects: adults of mayflies, stoneflies and caddisflies. Zenkoku Noson Kyoiku Kyokai Co., Ltd., Tokyo, 488 pp.","Navas, R. P. L. (1923) Algunos insectos del Museo de Paris. Plecopteros. Revista de la Academia de Ciencias Exactas, Fisicas, Quimicas y Naturales de Zaragoza, 7, 15 - 51."]}

A new genus of family Perlodidae, Tibetisoperla Huo & Du, gen. nov. is described with one new species from Tibet, China. Male of the new genus is characterized by the divided tergum 10 with several large apical erect setae, highly reduced vesicle on sternum 8, and the sclerotized paraprocts with separate apical sclerite.

Tibetisoperla wangluyui Huo & Du, sp. nov. (Figs. 1–11) Adult habitus: General color dark brown to black (Figs. 1, 8). Head mostly dark brown except the occiput paler. Triocellate, anterior ocellus smaller than posterior ones (Figs. 2A, 9A). Antennae and palpi dark brown. Pronotum disc rectangular (length/width = 1/1.4), black laterally and dark brown medially; rugosities present laterally (Figs.2A, 9A). Legs dark brown (Figs. 1, 8). Wings hyaline. Veins and thoracic sterna similar to Isoperla, typical of this subfamily (Figs. 2A, 3A–B). Male: Forewing length 7.2 mm, hindwing length 6.1 mm, body length 8.5 mm. Abdomen dark brown to black and slightly sclerotized. Terga 3-6 with a pair of small yellow spots medially (obscure in some individuals) (Fig. 3C). Terga 8–9 bearing paired patches of sensilla on the posterior margin. Terga 9–10 with a conspicuous pale medial line and dark longitudinal marks between the sensilla basiconica patches. Posterior half of tergum 10 bilobate, tips curved upwards with sensilla basiconica and several thicker and large setae (Figs 4A–D). Vesicle reduced to a dark semicircle on posterior margin of sternum 8 (Fig. 4E–F). Paraprocts developed and sclerotized, apex with a separate hemispheric sclerite with patch of spines (Figs. 5–6). Aedeagus columnar, membranous, covered by fine asperities but lack large sensilla or spines; apex slightly pointed, with a hemispheric transparent area on its tip (Fig. 7). Female: Forewing length 10.7 mm, hindwing length 9.1 mm, body length 10.0 mm. Body coloration similar to male (Figs. 8, 9A). Tergum 10 without sclerotized or special process. Subgenital plate broadly semicircular and sclerotized (length/width = 1/2.8), with an indistinct median notch (Fig. 9B–C). Egg: 191 µm wide, 271 µm long, including 10 µm for collar. Oval, posterior (opercular) pole regularly rounded, wide, sides of egg less arched near collar, narrowing a little towards anterior pole with collar. Chorion uniformly covered with irregular papillae. Collar 44 µm wide at recurved, wavy distal flange, with a few longitudinal crests, base deeply embedded. Anchor fungiform, evenly covered by globular bodies (Fig. 10). Nymph: Unknown. Type material: Holotype male: Tibet Autonomous Region, China: XZCQ-20-96, Meiduo Village, Quluo Township, Coqen County (riverside grassland, under the rocks) 2020-VII-29, 30°39′19.43′′ N, 85°7′54.62′′ E, 4751 m. Paratypes: Tibet Autonomous Region, China: 1♀, XZZB-20-66, Longdangbo, Qiongguo Township, Zhongba County, 2020-VII-23, 29°46"10.10" N, 83°55"36.31" E, 4556 m; 1♂, XZRT-20-79, Rutog Town, Rutog County, 2020-VII-25, 33°18′7.76′′ N, 79°41′29.87′′ E, 4288 m; 2♂♂, XZGE-20-83, Jiamu River (riverside grassland, under the rocks), Shiquanhe Town, Gar County, 2020-VII-27, 32°26"9.80" N, 80°12"12.89" E, 4340 m; 1♀, XZGJ-20-88, Qusuoma (riverside grassland), Xiongba Township, Ge'gyai County, 2020-VII-28, 32°5′44.16′′ N, 81°47′30.80′′ E, 4607 m; 2♂♂, XZCQ-20-96, Meiduo Village, Quluo Township, Coqen County (riverside grassland, under the rocks) 2020-VII-29, 30°39′19.43′′ N, 85°7′54.62′′ E, 4751 m. Leg. Wang Lu-Yu, Liu Piao, Yuan Tao, Hou Yan-Meng. Distribution: China: Tibet (Fig. 11). Etymology: The name of the species honors the collector, Mr. Wang Lu-Yu, a young researcher who is working on the taxonomy of Chinese spiders. Remark: Female of this species have no any distinctive generic character, and similar to other Isoperlinae genera., Published as part of Huo, Qing-Bo & Du, Yu-Zhou, 2021, A new genus of Isoperlinae (Plecoptera: Perlodidae) from Tibet, China, pp. 343-352 in Zootaxa 4996 (2) on pages 344-350, DOI: 10.11646/zootaxa.4996.2.8, http://zenodo.org/record/5070043

Tibetisoperla Huo & Du, gen. nov. Diagnosis: Small size, body length ca. 10 mm; macropterous, wing length 10–15 mm, general color dark brown or black. Triocellate, anterior ocellus smaller than posterior ones. Pronotum dark-colored medially with rugosities, with a smooth longitudinal band along the centerline. Wings hyaline. Terga 8–9 of male sclerotized posteriorly, bearing paired patches of sensilla; posterior half of tergum 10 bilobate, tip curved upwards with several large setae; vesicle on sternum 8 reduced; paraprocts developed and sclerotized, with a spiny apical sclerite. Aedeagus membranous, short columnar, without any sclerites, covered by fine spines. Type species: Tibetisoperla wangluyui Huo & Du, sp. nov. Monotypic. Etymology: This name combines " Tibet " and " Isoperla ", meaning "distributed in Tibet, similar to Isoperla "., Published as part of Huo, Qing-Bo & Du, Yu-Zhou, 2021, A new genus of Isoperlinae (Plecoptera: Perlodidae) from Tibet, China, pp. 343-352 in Zootaxa 4996 (2) on pages 343-344, DOI: 10.11646/zootaxa.4996.2.8, http://zenodo.org/record/5070043