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Additional file 2: Figure S2. a qRT-PCR confirming knock down of TFR1 (Hs_TFR1_5) at RNA level on MDA-MB-231 cells at different time points. b Igg-PE was used as control at different TFR1 knock down time points. Igg-PE signal is overlapping and displaying no fluorescent shift (right panel).

Additional file 3: Figure S3. LRC- HATRIC experiment comparing TRFE-HATRIC and EGFR Ab-HATRIC samples on MDA-MB-231 cells (left panel) and INS-HATRIC and TRFE-HATRIC samples on HEK293 cells (right panel). Data is shown at the protein level and proteins were annotated using the Uniprot database. Y axis = − Log10 (adj. p value), X-axis = log2 fold change compared to the other sample.

Additional file 1: Figure S1. a Representation of HATRIC, TriCEPS v.2.0 and TriCEPS–TAMRA molecules. b TriCEPS–ligand conjugates bind to the cell surface on mildly oxidized MDA-MB-231 cells to the same extend (left panel). TriCEPS coupled ligand binds only to the target receptor on non-oxidized cells (right panel). A representative experiment out of 3 is shown.