1. Serological responses in rabbits used to maintain uninfected, laboratory-reared tsetses (Glossina morsitans morsitans Westwood) (Diptera: Glossinidae)
- Author
-
Parker Kr
- Subjects
Male ,Immunodiffusion ,Tsetse Flies ,Immunoelectrophoresis ,Salivary Glands ,Serology ,Antigen ,medicine ,Animals ,Ecology, Evolution, Behavior and Systematics ,biology ,Salivary gland ,medicine.diagnostic_test ,Insect Bites and Stings ,Hemagglutination Inhibition Tests ,Precipitin ,Virology ,medicine.anatomical_structure ,Sephadex ,Antibody Formation ,biology.protein ,Animal Science and Zoology ,Female ,Rabbits ,Antibody - Abstract
Antibodies, reacting with homogenatesof salivary glands, were produced in rabbits exposed to Glossina morsitans morsitans Westwood. Precipitating antibodies and high titres of haemagglutinating antibodies occurred in all exposed rabbits. Precipitating antibodies, identified using immunoelectrophoresis, immunodiffusion, and precipitin ring tests, developed within 11 days of exposure. As many as seven antigen–antibody precipitin arcs were identified using immunoelectrophoresis. All precipitating antigens in the salivary glands had molecular weights greater than 25 000 (determined by Sephadex gel filtration); the salivary gland anticoagulant was not shown to be antigenic. No precipitating immunological reaction occurred between rabbit sera and tsetse hindguts or midguts. Titres of sera from rabbits receiving a second exposure to tsetses, following a period of no exposure, followed an anamnestic response.
- Published
- 1979