3 results on '"Pacile, S."'
Search Results
2. Hard and soft X-ray imaging to resolve human ovarian cortical structures
- Author
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Lorella Pascolo, Gabriella Zito, Geroge Kourousias, Giuliana Tromba, Marina Zweyer, Serena Pacilè, Alessandra Gianoncelli, Giuseppe Ricci, Regina C. Barroso, Alice Cernogoraz, G. Sena, Brian D. Metscher, Federico Romano, Pascolo, L., Sena, G., Gianoncelli, A., Cernogoraz, A., Kourousias, G., Metscher, B. D., Romano, F., Zito, G., Pacile, S., Barroso, R., Tromba, G., Zweyer, M., and Ricci, G.
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0301 basic medicine ,Nuclear and High Energy Physics ,Materials science ,Synchrotron radiation ,Reproductive technology ,Phase contrast tomography ,Synchrotron ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,law ,Humans ,Instrumentation ,Microtomography ,Ovary ,STXM ,Female ,Synchrotrons ,X-Ray Microtomography ,X-Rays ,Soft x ray ,030219 obstetrics & reproductive medicine ,Radiation ,Resolution (electron density) ,Phase-contrast imaging ,030104 developmental biology ,Beamline ,Tomography ,Human ,Biomedical engineering - Abstract
Laboratory and synchrotron X-ray tomography are powerful tools for non-invasive studies of biological samples at micrometric resolution. In particular, the development of phase contrast imaging is enabling the visualization of sample details with a small range of attenuation coefficients, thus allowing in-depth analyses of anatomical and histological structures. Reproductive medicine is starting to profit from these techniques, mainly applied to animal models. This study reports the first imaging of human ovarian tissue where the samples consisted of surgically obtained millimetre fragments, properly fixed, stained with osmium tetroxide and included in epoxydic resin. Samples were imaged by the use of propagation phase contrast synchrotron radiation micro-computed tomography (microCT), obtained at the SYRMEP beamline of Elettra light source (Trieste, Italy), and X-ray absorption microCT at the Theoretical Biology MicroCT Imaging Laboratory in Vienna, Austria. The reconstructed microCT images were compared with the soft X-ray absorption and phase contrast images acquired at the TwinMic beamline of Elettra in order to help with the identification of structures. The resulting images allow the regions of the cortex and medulla of the ovary to be distinguished, identifying early-stage follicles and visualizing the distribution of blood vessels. The study opens to further application of micro-resolved 3D imaging to improve the understanding of human ovary's structure and support diagnostics as well as advances in reproductive technologies.
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- 2019
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3. Advancements towards the implementation of clinical phase-contrast breast computed tomography at Elettra
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Bruno Golosio, Viviana Fanti, Angelo Taibi, Renata Longo, Maria Assunta Cova, Adriano Contillo, Paolo Russo, Luigi Rigon, Francesca Di Lillo, Piernicola Oliva, Luca Brombal, Diego Dreossi, Deborah Bonazza, Fabrizio Zanconati, Antonio Sarno, Maura Tonutti, Vittorio Di Trapani, Christian Fedon, Ubaldo Bottigli, Pasquale Delogu, Fulvia Arfelli, Sandro Donato, Giuliana Tromba, Giovanni Mettivier, Serena Pacilè, Longo, Renata, Arfelli, Fulvia, Bonazza, Deborah, Bottigli, Ubaldo, Brombal, Luca, Contillo, Adriano, Cova, Maria A., Delogu, Pasquale, Di Lillo, Francesca, Di Trapani, Vittorio, Donato, Sandro, Dreossi, Diego, Fanti, Viviana, Fedon, Christian, Golosio, Bruno, Mettivier, Giovanni, Oliva, Piernicola, Pacilè, Serena, Sarno, Antonio, Rigon, Luigi, Russo, Paolo, Taibi, Angelo, Tonutti, Maura, Zanconati, Fabrizio, Tromba, Giuliana, Longo, R., Arfelli, F., Bonazza, D., Bottigli, U., Brombal, L., Contillo, A., Cova, M. A., Delogu, P., Di Lillo, F., Di Trapani, V., Donato, S., Dreossi, D., Fanti, V., Fedon, C., Golosio, B., Mettivier, G., Oliva, P., Pacile, S., Sarno, A., Rigon, L., Taibi, A., Tonutti, M., Zanconati, F., and Tromba, G.
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Nuclear and High Energy Physics ,single photon counting ,breast CT ,phase contrast ,free space propagation ,Image quality ,Computer science ,Phase contrast microscopy ,Socio-culturale ,Computed tomography ,030218 nuclear medicine & medical imaging ,law.invention ,Conventional mammography ,03 medical and health sciences ,0302 clinical medicine ,law ,Cadmium Compounds ,medicine ,Humans ,Mammography ,Microscopy, Phase-Contrast ,Instrumentation ,Radiation ,medicine.diagnostic_test ,business.industry ,Detector ,Contrast resolution ,X-Ray Microtomography ,Women's cancers Radboud Institute for Health Sciences [Radboudumc 17] ,030220 oncology & carcinogenesis ,Female ,Tomography ,Tellurium ,Nuclear medicine ,business ,Synchrotrons - Abstract
Item does not contain fulltext Breast computed tomography (BCT) is an emerging application of X-ray tomography in radiological practice. A few clinical prototypes are under evaluation in hospitals and new systems are under development aiming at improving spatial and contrast resolution and reducing delivered dose. At the same time, synchrotron-radiation phase-contrast mammography has been demonstrated to offer substantial advantages when compared with conventional mammography. At Elettra, the Italian synchrotron radiation facility, a clinical program of phase-contrast BCT based on the free-space propagation approach is under development. In this paper, full-volume breast samples imaged with a beam energy of 32 keV delivering a mean glandular dose of 5 mGy are presented. The whole acquisition setup mimics a clinical study in order to evaluate its feasibility in terms of acquisition time and image quality. Acquisitions are performed using a high-resolution CdTe photon-counting detector and the projection data are processed via a phase-retrieval algorithm. Tomographic reconstructions are compared with conventional mammographic images acquired prior to surgery and with histologic examinations. Results indicate that BCT with monochromatic beam and free-space propagation phase-contrast imaging provide relevant three-dimensional insights of breast morphology at clinically acceptable doses and scan times.
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- 2019
- Full Text
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