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2 results on '"PHYTOSTERYL ESTER"'

1. Substrate-Specificity of Candida rugosa Lipase and Its Industrial Application

Author

Sofie Winderickx, Karin Thevissen, Bruno P. A. Cammue, Bert Lagrain, Evelien Vanleeuw, Bert F. Sels, and Michiel Dusselier

Subjects
Technology, BIOCHEMICAL-CHARACTERIZATION, substrate specificity, Chemistry, Multidisciplinary, General Chemical Engineering, 02 engineering and technology, 01 natural sciences, chemistry.chemical_compound, Engineering, GERANYL PROPIONATE, Food science, Green & Sustainable Science & Technology, biology, food and beverages, 021001 nanoscience & nanotechnology, Candida rugosa lipase (CRL), Lactic acid, Candida rugosa, Chemistry, LIP2 LIPASE, Biodiesel production, Physical Sciences, Science & Technology - Other Topics, 0210 nano-technology, Engineering, Chemical, immobilization methods, Immobilized enzyme, macromolecular substances, 010402 general chemistry, Kinetic resolution, Pichia pastoris, Lip isoenzymes, BIOCATALYTIC PRODUCTION, PHYTOSTERYL ESTER, Environmental Chemistry, Lipase, BIODIESEL PRODUCTION, Aroma, LACTIC-ACID, Science & Technology, Renewable Energy, Sustainability and the Environment, fungi, technology, industry, and agriculture, General Chemistry, biology.organism_classification, 0104 chemical sciences, PICHIA-PASTORIS, ENZYME IMMOBILIZATION, chemistry, biology.protein, industrial applications, KINETIC RESOLUTION
Abstract

Candida rugosa lipase (CRL) is a very versatile and widely used lipase in the aroma and flavor industry, the fat and oil industry, the pharmaceutical industry and is also applicable as a biosensor....

Published
2019
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2. Enzymatic strategies for solvent-free production of short and medium chain phytosteryl esters

Author

Guillermo Reglero, Carlos F. Torres, Guzman Torrelo, Comunidad de Madrid, and Ministerio de Ciencia e Innovación (España)

Subjects
chemistry.chemical_classification, Solvent free, Pseudomonas stutzeri TL lipase, General Chemistry, Candida rugosa lipase, Biology, Butyric acid, Industrial and Manufacturing Engineering, chemistry.chemical_compound, Enzyme, chemistry, Chain (algebraic topology), Organic chemistry, Phytosteryl ester, Food Science, Biotechnology
Abstract

Transesterification of phytosterols with short and medium chain fatty acids (MCFAs) ethyl esters and with tributyrin catalyzed by Candida rugosa and Pseudomonas stutzeri TL lipases in solvent-free medium was studied including the effects of temperature, fatty acid specificity, enzyme quantity, the residual activity of each lipase and reduced pressure. More than the 90% of the original phytosterols were transformed into their corresponding phystosteryl esters after 3-4h reaction at 50-60°C and at 100-350mbar. The product mixtures containing 40-50% (w/w) phystosteryl esters were then vacuum distilled to remove molar excess of SCFA and MCFA ethyl esters utilized in the enzymatic reaction. By this methodology, purities >95% in short and medium chain phytosteryl esters were finally obtained. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim., Funded by: Comunidad Autónoma de Madrid (ALIBIRD. Grant Number: S2009/AGR-1469 and Consolider-Ingenio FUN-C-FOOD. Grant Number: CSD2007-00063.

Published
2012

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