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Artemisia annua L. is a medicinal plant appreciated for the production of artemisinin, a molecule used for malaria treatment. However, the natural concentration of artemisinin in planta is low. Plant nutrition, in particular phosphorus, and arbuscular mycorrhizal (AM) fungi can affect both plant biomass and secondary metabolite production. In this work, A. annua plants were ino- culated or not with the AM fungus Funneliformis mosseae BEG12 and cultivated for 2 months in controlled conditions at three different phosphatic (P) concentrations (32, 96, and 288 µM). Plant growth parameters, leaf photosynthetic pigment concentrations, artemisinin production, and mineral uptake were evaluated. The different P levels significantly affected the plant shoot growth, AM fungal colonization, and mineral acquisition. High P levels negatively influenced mycorrhizal colonization. The artemisinin concentration was inversely correlated to the P level in the substrate. The fungus mainly affected root growth and nutrient uptake and significantly lowered leaf artemisinin concentration. In conclusion, P nutrition can influence plant biomass production and the lowest phosphate level led to the highest artemisinin concentration, irrespective of the plant mineral uptake. Plant responses to AM fungi can be modulated by cost–benefit ratios of the mutualistic exchange between the partners and soil nutrient availability.

The critical value of soil Olsen-P (mg Olsen-P kg-1) is the value of soil Olsen-P above which the probability of crop response to P fertilization is small or null. The aim of the present study was to determine the critical value of soil Olsen-P for potato tuber yield in volcanic soils. The data used in this study were taken from 41 experiments of P fertilization conducted in southern Chile from 1977 to 2015 by the National Potato Center at the Instituto de Investigaciones Agropecuarias (INIA). To identify the critical values of soil Olsen-P, relationships between relative yields and initial soil P-Olsen were assessed using three models (linear-plateau, boundary and Mitscherlich function). Across experiments, the average fresh tuber yield was 41.9 Mg ha-1 and varied between 4 and 89 Mg ha-1. Fresh tuber yield decreased up to 92% by P deficiency; however, yield reduction was concentrated between 20 and 40%. The critical Olsen P values determined were 41, 33 and 11 mg Olsen-P kg-1 obtained (P < 0.01) with linear-plateau, boundary function and Mitscherlich function, respectively. However, linear-plateau and Mitscherlich functions showed lower coefficient of determinations (R2 = 0.23 - 0.31) than the boundary function (R2 = 0.97). Therefore, we suggest that the critical Olsen-P value of 33 mg P kg-1 determined with the boundary function is more appropriate for potato growers than the critical value determined with the other models. This information will be valuable for optimizing strategies for P fertilizer management in potato production systems aimed at achieving optimal crop yield and minimizing potential environmental risk.