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Onion seeds are prone to rapid germination and viability losses under unfavourable storage conditions. The final germination percentage is considered the most important parameter for determining the performance of seed lots after storage, although other quantitative traits, such as the time and speed of germination, help in more realistic predictions of seed germination. A study was conducted on seventeen seed lots of onion seeds to delineate the most comprehensive parameter indicating seed performance after storage using the four-parameter Hill function (4-PHF) mathematical model. Seeds of seventeen onion cultivars were subjected to accelerated ageing at 42 °C and 100% RH for 48, 96 and 144 h, followed by seed germination evaluation. The germination performance was evaluated by 4-PHF based on time-related parameters, such as the time to maximum germination rate (TMGR), time to 50% germination (T50), and uniformity (U), along with the germination percentage (a), shape and steepness of the Four-Parameter Hill Function (FPHF) curve (b), which were important determinants of the area under curve (AUC), and RoG (rate of germination) curves. Among the parameters, the AUC was found to provide the most comprehensive evaluation of the storage performance of the onion varieties and was decisive in the classification of the varieties as ‘good’ or ‘poor’ storers. A positive correlation between seed vigour index-I (SVI-I) and the AUC reiterated the suitability of using 4-PHF parameters for the assessment of the storage potential of onion varieties.

The objective of this study was to evaluate the effect of dietary onion (Allium cepa) and ginger (Zingiber officinale) supplementation on growth, carcass quality, antioxidant status, and immune response in broilers under heat stress. A total of 700 day-old Hubbard broiler chicks were weighed and assigned to five treatments and five replicates. Broilers were maintained in a thermoneutral (TN) environment or were exposed to heat stress (HS). For 35 days, HS birds were fed a control diet and three levels of onion and ginger powder as: 5 g/kg ginger + 1.5 g/kg onion (T1), 10 g/kg ginger + 2.5 g/kg onion (T2), and 15 g/kg ginger + 3.5 g/kg onion (T3). Body weight, feed conversion ratio (FCR), dressing percentage, and the weight of immune-related organs improved in T2 compared to the control. Blood concentrations of malondialdehyde (MDA) and paraoxonase-1 (PON1) were substantially higher in T2 than the control. Similarly, in the same dietary group, the antibody titre against Newcastle disease (ND) and total leucocyte count (TLC) were greater than the control diet. The findings of this research indicate that 10 g of ginger combined with 2.5 g of onion in the diet enhance broiler growth performance, carcass quality, antioxidant status, and immunological response under heat stress conditions.&nbsp

Soil fertility depletion is a serious problem in Ethiopian highland due to leaching of topsoil by runoff, which reduces production and productivity. This experiment conducted in Debub Ari district for two years with the aim of investigating single and combined effect of compost and FYM with inorganic fertilizer on soil fertility improvement and onion crop production. The treatment includes T1) Control, T2) Recommended NP (69/30), T3) NPSZnB (120N, 92P2O5, 20.06S, 5.89Zn, 0.66B) kg ha-1(264 kg NPSZnB + 161 kg urea), T4) 10 t ha-1FYM, T5) 10 t ha-1compost, T6) 5 tha-1FYM + 50% of treatment-3 and T7) 5 t ha-1compost + 50% of treatment-3. Treatments are arranged in randomized complete block design with three replications. Soil before the experiment showed moderately acidic, moderate in organic matter and boron, low in sulfur high in available P and total nitrogen. The results showed significant differences among the treatments on onion plant height, bulb diameter, marketable and biological yield. The highest total yield 19.99 t ha-1, 19.59 t ha-1and 19.25 t ha-1was recorded from NPSZnB (120N, 92P2O5, 20.06S, 5.89Zn, 0.66B) kg ha-1, 5 t ha-1compost + 50% of T-3 and 5 t ha-1FYM + 50% of T-3 respectively and that increase yield by 39%, 35% and 33% over the control. Soils after the experiment become medium in sulfur remain static in available boron and total nitrogen. Economic feasibility that the maximum net benefit of 246059 and 242442 ETB ha-1with acceptable %MRR of 290 and 1300 was obtained with the application of NPSZnB (120N, 92P2O5, 20.06S, 5.89Zn, 0.66B) kg ha-1and 5 t ha-1FYM + 50% of T-3, respectively. Therefore, the application of those two treatments is recommended for higher yield production in the study area.

The study aimed to evaluate the effect of onion-garlic powder on egg production, antioxidant capacity and blood biochemistry in chukar partridges (Alectoris chukar). The study was conducted for 12 weeks during the laying period and a total of 240 partridges were divided into 4 groups (control, 1% onion, 1% garlic, and 1% onion and 1% garlic). The highest total antioxidant capacity, feed consumption and egg production were determined in the garlic group (P < 0.05 and P < 0.01 respectively) and the lowest egg production and feed intake was observed in the onion group. Egg production in the garlic –onion mixture group was also higher than the control and only onion group (P < 0.01). While there was no statistical difference in blood HDL, LDL, glucose, alkaline phosphatase, calcium, and phosphorus levels (P > 0.05), blood urea, ALT, AST levels were higher (P < 0.05) in the garlic group with the highest egg production and feed intake (P < 0.01) compared to the control and onion groups. Unlike many studies, the highest cholesterol levels were determined in the garlic supplemented group (P < 0.05) and the expected positive effects were not seen in onion powder.

In July 2019, severe leaf symptoms were observed on onion plants (Allium cepa L. cv. Dorata di Parma) in a commercial field located in the municipality of Medicina (Bologna, Emilia-Romagna region), in northern Italy. Diseased leaves showed yellowish-pale-brown and oval-shaped lesions, which later coalesced in larger necrotic areas, and black leaf tips. As the disease progressed, conidia developed on the necrotizing leaves, until premature desiccation of the whole plants. Disease incidence of approximately 70% was calculated in the affected field, together with yield losses that were estimated to be above 30%. Symptomatic tissue fragments excised from the leaf lesions were surface disinfested with NaOCl 1% for 2 minutes, rinsed with sterile water and transferred onto potato dextrose agar (PDA). Fungi were consistently isolated after 5 days of incubation at 27 ± 1°C in the dark. Single spore isolation was performed on PDA to obtain 7 pure cultures, whose morphological characteristics were consistent with the description of Stemphylium vesicarium (Ellis 1971). DNA from a representative single spore isolate was extracted and the internal transcribed spacer region (ITS) of ribosomal DNA (rDNA) was amplified using the universal primers P-ITS1 and P-ITS4 (White et al. 1990). The PCR product was sequenced and deposited in GenBank (Accession No. OP144057). A BLAST search in CBS-KNAW collection bank (Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands) showed 100% identity for the ITS gene with the strain of S. vesicarium under accession number CBS 124749. Moreover, the PCR assay using the primer pair KES 1999 and KES 2000 (Graf et al. 2016) for the cytochrome b gene displayed the specific fragments of 420 bp for S. vesicarium. The isolate was tested for pathogenicity on onion (potted plants cv. Texas Early Gran, fourth leaf stage) by spraying 4 ml of a conidial suspension (1 × 104 conidia/ ml) per plant. Inoculated and non-inoculated (sprayed with sterile distilled water) plants were kept at 24 ± 1°C and 90% relative humidity with a 16-h photoperiod. Seven days after inoculation, disease assessment was performed. Inoculated plants showed typical Stemphylium leaf blight (SLB) symptoms, similar to those observed in the field. No symptoms developed on the water-inoculated plants. S. vesicarium was consistently reisolated from the artificially inoculated onion plants and identified using a PCR assay, according to Graf et al. (2016). The assay was repeated twice with the same results. SLB is currently reported worldwide and it is considered a re-emerging threat and a truly challenging fungal disease, which can result in yield and quality losses of up to 90% in onion crops (Hay et al. 2021). In Italy, S. vesicarium has been reported several years ago on pear (Ponti et al. 1982) and, more recently, on radish sprouts (Belisario et al. 2008), chili pepper (Vitale et al. 2017) and spinach (Gilardi et al. 2022). To our knowledge, this is the first report of S.vesicarium on onion in Italy. Our results stress that development and implementation of innovative Integrated Pest Management (IPM) strategies are urgently needed to ensure an effective control of SLB, since only a few moderately resistant onion varieties are available (Hay et al. 2021) and no fungicides are currently registered to specifically control SLB in Italy. Further studies are underway to elucidate the pathogen geographic distribution and assess the impact of this disease on the onion crop in Italy.

The experiment was conducted to study the response of summer onion to N2and Naphthalene Acetic Acid (NAA). The experiment consisted of two factors. Factor A: 4 levels of Nitrogen viz. N0-0kg (control), N1-90kg ha-1, N2-110kg ha-1and N3-130kg ha-1and Factor B: 4 levels of NAA viz. H0-0ppm, H1- 50ppm, H2-100ppm and H3-150ppm. The experiment was laid out in Randomized Complete Block Design with three replications. Results revealed that in terms of nitrogen, the highest bulb diameter (4.90cm), bulb weight plant-1(51.48g) and bulb yield ha-1(17.78t) were found from N2(110kg N ha-1). In case of application of NAA, the highest bulb length (3.22cm), bulb diameter (4.38cm), bulb weight plant-1(45.18g) and bulb yield ha-1(15.49t) were recorded from H2(100ppm NAA). Regarding combined effect of nitrogen and NAA the highest bulb length (3.76cm), highest bulb diameter (5.26cm), highest bulb weight plant-1(55.50g) and highest bulb yield ha-1(19.60t) were recorded from the treatment combination of N2H2. Considering economic analysis, the highest BCR (2.39) was also obtained from N2H2(110kg N ha-1with 100ppm NAA) treatment combination.

The present study was carried out to assess the potential radiation hazards to the public, especially the persons who consumed onion and garlic plants widely during coronavirus disease in Thi Qar province South of Iraq. Nine samples collected from the market (5 samples onion and 4 sample Garlic), which classified according to their origin. Using 3″x3″ NaI (Tl) gamma ray spectroscopy system, the radioactivity concentrations of the natural radionuclides radium-226, thorium-232 and potassium-40 were determined. The results obtained showed that the average concentration of radioactivity of radium-226, thorium-232 and potassium-40 is 3.398 Bqkg−1, 4.667 Bqkg−1 and 216.738 Bqkg−1, respectively, for onion and 2.808 Bqkg−1, 3.524 Bqkg−1, and 172.064 Bqkg−1 for garlic. The results also showed that the average annual total effective dose of the three nuclides is 122.955 μSv.y-1 for onion and 97.231 μSv.y-1for garlic. Other relevant risk parameters were also calculated, such as equivalent activity concentrations, absorbed dose, excess lifetime cancer risks, and other health risk parameters. One of the most important conclusions reached by this study is that the natural radioactive elements in onions and garlic do not pose a great danger to their consumers, especially those infected with the COVID-19. Because the concentrations of these radioactive elements do not exceed the permissible limits recommended by recognized scientific organizations and agencies such as International Commission on Radiological Protection (ICRP), United Nations Scientific Committee on the Effects of Atomic Radiation (UNSCEAR), and World Health Organization (WHO).

VOC database for aspergillus infection in onion

Received: February 9th, 2023 ; Accepted: April 25th, 2023 ; Published: April 27th, 2023 ; Correspondence: liga.lepse@llu.lv Some scientific reports support the idea of using plant interactions to promote the growth and yielding of vegetable crops. The plant interactions in vegetable production under intercropping conditions were investigated in ERDF funded project ‘Elaboration of environment-friendly crop growing technologies identified by the Green Deal and their implementation in horticultural production in Latvia (GreenHort)’ implemented in Latvia Institute of Horticulture with the aim to introduce strip cropping in the vegetable production. The investigations were carried out at the Institute of Horticulture, Latvia (57°03’44.6’’N, 22°54’53.2’’E), during the growing seasons of 2021 and 2022. The vegetable crops (carrots, cabbage, onions, and pumpkins) were grown in intercropping with agroecological service plants or aromatic plants as companion plants (white clover, marigolds, tagetes, lavender, sage, coriander). The investigated variants were compared with the control, where vegetable crops were grown in monoculture as usual. Each intercrop variant consists of 7 alternating rows (each 0.6 m wide) - 4 rows of service crop and 3 rows of vegetable. There was observed significant influence of the growing system on the plants productivity. The sharpest differences between variants were observed for cabbage - marigold, sage and lavender had a positive influence on the cabbage yield formation, but white clover had an extremely negative influence on the cabbage plant growth. White clover had a negative influence also on carrot and pumpkin productivity. Sage had a yield-promoting influence on the carrot crop. There was not found any significant influence of the agroecologial crops on onion productivity.

The most important factors are the seed quality and vigor in which determine the yield and quality of vegetable production. Seed quality is affected by many factors such as seed development on the mother plant, genetic characters of species, and variety, storage conditions, etc. Onion (Allium cepa L.) has a seed that is easily deteriorating. Therefore one-year seed use is commonly recommended. In this research, which was conducted to determine the effect of the production year and variety on seed emergence and seedling properties was used one- and the two-year seed of four long-day variety (denotes A, B, C or D). As result, it was determined that the seedling morphological characters were not affected by production year and one-year seeds had a higher emergence percentage and vigor index than two-year seeds. The highest emergence rates were seen in variety D (95.50%) for one-year seeds and in variety A (80.00%) for two-year seeds. Among vigor indexes, the highest values were observed in variety C (2.85) for one-year seeds and variety A (2.39) for two-year seeds. Also, seed viability and viability decreasing rate were different according to varieties.

Modelling and optimization represent an important aspect of drying in food processing, providing a fast and convenient means for quality prediction. The research focuses on modelling and optimization of process parameters such as drying rate, water loss, solid gain, vitamin C, manganese, and iron of dried osmo-pretreated onion slices. Least square regression analysis in the Math-lab computer software was used to model and optimise the process parameters., Six (6) mathematical models were developed for each output from the regression analysis that was carried out. The criteria for adjudging these models were the values of their adjusted coefficient of multiple determinations, prediction error sum of squares (also called deleted residual), R2 for prediction, coefficient of variation CV, and the Dubin-Watson test for autocorrelation. The models were checked for adequacy using these criteria, and those found to be adequate were selected from among the other possible combinations. Hence, the best-optimized obtained results from the models are 27.50 g/h, 1.61 g/g, 0.15 g/g, 77.52 mg/100 g, 2.79 mg/1000 g, and 2.19 mg/1000 g for drying rate, water loss, solid gain, vitamin C, manganese, and iron, respectively.

In this work, the intensification of the extraction of flavonoids from dry onion skin wastes (DOSW) by Ultrasound Assisted Extraction (UAE) was investigated. Conventional stirred tank extraction was used to find the optimal temperature (37 °C) and solvent (ethanol 70%, v/v), providing 20.7 ± 0.4 mg QE/g DOSW after 1 h. In the UAE process, the amplitude of oscillation and the solvent-to-solid ratio were key parameters to control the specific energy used. Energies above 10 kJ/g OSW did not increase the extraction yield (maximum flavonoids content was 23.9 ± 0.2 mg QE/g DOSW), which could be obtained using amplitudes of 40%, high solvent-to-solid ratios (30 mL/g DOSW) and extraction times below 5 min. Although both extraction techniques provided similar profiles of phenolic compounds, being quercetin and quercetin-4′-O-glucoside the main flavonoids (7.4 mg/g DOSW and 10.4 mg/g DOSW, respectively), UAE increased the productivity up to 11.6 mg QE/(g DOSW·min), seven times higher than conventional extraction. The extraction kinetics was modeled and studied. The optimum extract was freeze-dried resulting in a solid powder rich in flavonoids (21%) which kept 90% of the antioxidant activity after 180 days of storage at temperatures below 4 °C., Junta de Castilla y León (JCyL) and the European Regional Development Fund (ERDF) [grant numbers BU301P18 and BU050P20] and the Agencia Estatal de Investigación [grant number PID2019-104950RB-I00/AEI/10.13039/501100011033 and PID2020-116716RJ-I00]. OBR’s postdoctoral contract through project BU301P18 is gratefully acknowledged. To JCyL and European Social Fund (ESF) for the contract of D. Benito-Bedoya and D. M. Aymara-Caiza through the YEI program, who participated in this work as technicians.

Aim: To evaluate the effects of fresh onion juice on female infertility. Background: Onion contains several antioxidants and has been used for the subfertile woman to achieve pregnancy by herbalists. Objective: This study was carried out to investigate the effects of fresh onion juice on ovarian folliculogenesis and implantation in rats. Methods: Female albino rats were divided into two groups: 5-day (one estrus cycle) and 10-day treatment (two estrus cycles) groups. The animals in the two groups were then divided into subgroups of the control group, 1 cc onion juice and 2 cc onion juice, each containing seven rats. At the end of the 5th and 10th days, ovarian volume and weight were measured, and the primordial, antral, and atretic follicles, and corpus lutea were counted. Vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) were evaluated both in the ovaries and in the endometrium immunohistochemically. Results: In the 5-day treatment group, ovarian weight and the antral follicle count were significantly higher in the 2 cc onion juice subgroup than the control group (p Conclusion: The results of our study showed that fresh onion juice could increase the number of antral follicles, which is an indicator of enhanced folliculogenesis.

This research about how to modify a machine that peels onions. The method used is Reverse engineering. This machine was modified because there is a shortage, level of success onion-peeler machine is still low, with value 43.63%, safety level machine is not sufficient, and the diameter of peeler tube hole is too large, so it is not effective and efficient. Modification made for to improve the efficiency of peeling shallots. The modified part is the rubber and the diameter of the peeler hole. Tube-hole size changed from 16mm to 6mm. will use the Shaft On the stripper tube. The shaft works like a holder for a rubber peeler. There are 11 pieces of peeling rubber. The results of the study showed that the peeled onion was 755 grams after 20 minutes. The machine's actual capacity is 37.75 grams per minute, or 2.26 kg per hour. The machine's production efficiency is 75.5%.

Relevance. Providing the country's population with a variety of vegetable products is the most important socio-economic task in the Republic of Belarus. It is necessary not only to increase the production of vegetables, but also to significantly expand their range. In connection with the trend of decline in industrial production of onions, the issue of ensuring the sowing of onions in the annual culture of seeds from domestic varieties and hybrids is relevant. Also in Belarus, the problem is the provision of garlic to the population of the country.Results. In the article summarizes the results of scientific research on the breeding and development of technologies for the cultivation of onion crops in Belarus, analyzed the main and promising areas of work. Data on the results of competitive sorting of new varieties and hybrids of onion and leek, the main technological parameters of growing garlic and leeks are given.

Relevance. In Uzbekistan, especially in the south of the republic, in recent years, early ripe varieties of onion Sumbula, Ravnak, Bakhora have become widespread. However, the technology of seed production of early maturing varieties has not been developed. In this regard, several studies were carried out to identify the optimal time for planting the mother plants of the early maturing onion variety Sumbula by using the favorable climatic conditions of southern Uzbekistan.Material and methodology. The material for the research was the early ripe onion variety Sumbula, bred by the Research Institute of Vegetable and Melon Crops and Potatoes. The studies were carried out according to the Methodological guidelines for the ecological testing of vegetable crops in the open field etc. The dates of seed planting were studied.Results. Studies have shown that the highest seed yield (0.97 t/ha) was obtained in the early autumn planting of mother plants on September 1. It was 20% more than in the control variant – September 15. At later planting dates, the seed yield decreases sharply.When mother plants were planted on October 30, the seed yield was only 0,46 t/ha, or 59.7% of the control.The yield of onion seeds increases, mainly due to the more powerful development of plants during early autumn planting – the formation of large leaves, inflorescences, and additional arrows. The more large and powerful leaves, the more plants have the ability to synthesize plastic substances in the process of photosynthesis and accumulate a high yield.

This article comments on: Oku S, Ueno K, Sawazaki Y, Maeda T, Jitsuyama Y, Suzuki T, Onodera S, Fujino K, Shimura H. 2022. Functional characterization and vacuolar localization of fructan exohydrolase derived from onion (Allium cepa). Journal of Experimental Botany 73,4908–4922. ispartof: JOURNAL OF EXPERIMENTAL BOTANY vol:73 issue:14 pages:4620-4623 ispartof: location:England status: published

Background Studies indicate that onion supplementation may be effective in the treatment of dyslipidemia; however, the results remain controversial. This meta‐analysis was conducted to evaluate potential benefits of onion on lipid profile. Methods Up to 12 October 2020, PubMed, Cochrane Library, Web of Science, and Scopus were searched for randomized controlled trials evaluating the effects of onion on lipid profile. Mean differences (MD) and 95% confidence intervals (CI) were calculated. Meta‐analysis was conducted using the fixed‐effects model. Results Ten trials with 446 participants in total were included in the meta‐analysis. The pooled findings of 10 studies suggested that onion supplementation significantly improved high‐density lipoprotein cholesterol (HDL) (MD: 2.29 mg/dl; 95% CI: 0.87, 3.72; I 2 = 0%) and low‐density lipoprotein cholesterol (LDL) (MD: −6.64 mg/dl; 95% CI: −10.91, −2.36; I 2 = 32%),while onion supplementation did not significantly lower triglycerides (TG) (MD: −6.55 mg/dl; 95% CI: −15.64, 2.53; I 2 = 45%). Analysis of nine trials showed a significant reduction in total cholesterol (TC) (MD: −5.39 mg/dl; 95% CI: −10.68, −0.09; I 2 = 49%) in patients with onion supplementation compared to the control group. Conclusion In summary, supplementation of onion was beneficial to control dyslipidemia, including improving levels of HDL, LDL, and TC, but could not reduce TG level. The therapeutic benefits of onion for dyslipidemia need to be treated with caution considering that some of the results are not robust., Cardiovascular diseases are, currently, the leading cause of death globally, accounting for about one third of all deaths. Controlling blood lipids can effectively reduce the risk of cardiovascular diseases. Onion supplementation was beneficial for blood lipids, including improving plasma levels of HDL, LDL, and TC.

Fusarium basal rot (FBR) of onion is a serious disease problem worldwide. The Fusarium species causing FBR can also produce mycotoxins that are potentially harmful to humans and animals. In this study, a multiple reaction monitoring technique with ultra-high-performance liquid chromatography–tandem mass spectrometry (MRM UHPLC-MS/MS) was developed and validated for onion matrix to study Fusarium mycotoxins in the harvested onions. This study was focused on fumonisins B1, B2, and B3 (FB1, FB2, and FB3), beauvericin (BEA), and moniliformin (MON), which are the main mycotoxins produced by Fusarium oxysporum and Fusarium proliferatum. In the in-house validated protocol, the onion samples were extracted with methanol:water (3:1) using magnetic stirring for 15 min. FBs and BEA were determined directly from the filtered extracts, whereas MON required sample concentration prior to analysis. No cleanup of extracts was needed prior to analysis. The target mycotoxins were separated on an Acquity UPLC system BEH C18 column with gradient elution. Mycotoxins were identified and quantified using 13C-FB1 as internal standard. Minor matrix effect was compensated using multi-point matrix-matched calibration curves with uninfected onion sample. For the mycotoxins studied, a good linearity was obtained (R2 ≥ 0.99) and the recoveries were in the range of 67–122%, with the highest standard deviation for MON, 22%. The limits of quantification were from 2.5 to 10 ng g−1 in onion matrix. The method was successfully employed for the analysis of mycotoxins in harvested onions showing FBR symptoms and found to be infected with F. oxysporum and F. proliferatum.

Throughout the last decade, and particularly in 2022, water scarcity has become a critical concern in Morocco and other Mediterranean countries. The lack of rainfall during spring was worsened by a succession of heat waves during the summer. To address this drought, innovative solutions, including the use of new technologies such as hydrogels, will be essential to transform agriculture. This paper presents the findings of a study that evaluated the impact of hydrogel application on onion (Allium cepa) cultivation in Meknes, Morocco. The treatments investigated in this study comprised two different types of hydrogel-based soil additives (Arbovit® polyacrylate and Huminsorb® polyacrylate), applied at two rates (30 and 20 kg/ha), and irrigated at two levels of water supply (100% and 50% of daily crop evapotranspiration; ETc). Two control treatments were included, without hydrogel application and with both water amounts. The experiment was conducted in an open field using a completely randomized design. The results indicated a significant impact of both hydrogel-type dose and water dose on onion plant growth, as evidenced by various vegetation parameters. Among the hydrogels tested, Huminsorb® Polyacrylate produced the most favorable outcomes, with treatment T9 (100%, HP, 30 kg/ha) yielding 70.55 t/ha; this represented an increase of 11 t/ha as compared to the 100% ETc treatment without hydrogel application. Moreover, the combination of hydrogel application with 50% ETc water stress showed promising results, with treatment T4 (HP, 30 kg, 50%) producing almost the same yield as the 100% ETc treatment without hydrogel while saving 208 mm of water.

The population is now more aware of their diets due to the connection between food and general health. Onions (Allium cepa L.), common vegetables that are minimally processed and grown locally, are known for their health-promoting properties. The organosulfur compounds present in onions have powerful antioxidant properties and may decrease the likelihood of developing certain disorders. It is vital to employ an optimum approach with the best qualities for studying the target compounds to undertake a thorough analysis of these compounds. In this study, the use of a direct thermal desorption–gas chromatography–mass spectrometry method with a Box–Behnken design and multi-response optimization is proposed. Direct thermal desorption is an environmentally friendly technique that eliminates the use of solvents and requires no prior preparation of the sample. To the author’s knowledge, this methodology has not been previously used to study the organosulfur compounds in onions. Likewise, the optimal conditions for pre-extraction and post-analysis of organosulfur compounds were as follows: 46 mg of onion in the tube, a desorption heat of 205 °C for 960 s, and a trap heat of 267 °C for 180 s. The repeatability and intermediate precision of the method were evaluated by conducting 27 tests over three consecutive days. The results obtained for all compounds studied revealed CV values ranging from 1.8% to 9.9%. The major compound reported in onions was 2,4-dimethyl-thiophene, representing 19.4% of the total area of sulfur compounds. The propanethial S-oxide, the principal compound responsible for the tear factor, accounted for 4.5% of the total area.

Вопрос о связях между центральнозиатскими хунну (сюнну) и восточноевропейскими гуннами, несмотря на большое количество посвященных этому работ, остается дискуссионным. В статье на основании археологических источников, рассматриваемых в историческом контексте, он решается положительно; устанавливается следующая последовательность его рассмотрения. Обосновывается понятие «хуннской триады» (определенные виды вооружения, украшений, бронзовые котлы), характеризующей ранжированную воинскую субкультуру хунну. Рассматриваются культурные особенности ближайшего окружения этносоциального объединения Хунну – Тува, Минусинская котловина, Горный Алтай (условно – «Ближний круг»). Приводятся археологические данные о распространении носителей этого культурного комплекса на территорию Западной Сибири (по Л.Н. Гумилеву), а именно саргатской культурно-исторической общности (условно – «Дальний круг»), ставшей местом исхода потомков хунну/гуннов при движении на Запад. Прослеживается сохранение элементов воинской субкультуры хунну в материалах восточноевропейских кочевников (по И.П. Засецкой), с заменой знаковых видов украшений – бронзовых изделий на изделия полихромного стиля. Впервые ставится вопрос о возможности «обратного» движения гуннов в сторону Центральной Азии, прослеживаемого по проявлениям прежних традиций, в первую очередь, по находкам полихромных изделий в ряде памятников Восточного Приуралья и Казахстана, Западной Сибири и Алтая, The question of the relationship between the Central Asian Huns (Xiongnu) and the Eastern European Huns, despite a large number of works devoted to this, remains debatable. In the article, based on the archaeological sources considered in the historical context, it is solved positively; the following sequence of its consideration is established. The author substantiates the concept of the “Hun triad” (certain types of weapons, jewelry, bronze cauldrons), which characterizes the ranked military subculture of the Hun. The article deals with the cultural features of the immediate environment of the ethnic and social Association of Hunnu-Tuva, Minusinsk basin, and Gorny Altai (conditionally – “near circle”). The article presents archaeological data on the spread of the carriers of this cultural complex to the territory of Western Siberia (according to L.N. Gumilyov), namely the Sargat cultural and historical community (conditionally-“far circle”), which became the place of Exodus of the descendants of the Xiongnu/Huns when moving to the West. The author traces the preservation of elements of the military subculture of the Huns in the materials ofEastern European nomads (according to I.P. Zasetsky), with the replacement of the iconic types of jewelry – bronze products to the product of the polychrome style. For the first time the article raises the question about the possibility of “reverse” movement of the Huns to Central Asia, followed closely by manifestations of old traditions, first and foremost, the findings of polychromatic products in a number of sites of the East of the Urals and Kazakhstan, Western Siberia and Altai.

The aim of this study was to investigate the effects of different harvest periods and drying methods on onion seed quality and yield. The umbels were harvested 35, 50 and 65 days after 50% of bloom on the umbels (DAB). Then, harvested umbels were dried in the sun and in the shade. Germination and controlled deterioration tests were used for determining the seed quality. The seed yield umbel-1 (g) and seed yield da-1 (kg) were calculated for yield evaluation. Drying methods affected on seed viability and the highest germination percentage (96.7%) was obtained from drying in the shade. The seed vigour did not significantly vary by harvest dates when umbels were dried in the shade. On the other hand, harvest dates following drying in the sun had significant effect on seed quality and the seeds harvested 50 DAB had the highest seed vigour (97%). Although there was no effect of harvest dates and drying methods on seed yield umbel-1 (g), drying in the shade had significant effect on seed yield da-1 (kg). © 2020 International Society for Horticultural Science. All rights reserved.

Genetic diversity and Agro-climatic conditions contribute significantly to the agronomic and morphological features of the food plant species, and their nutraceutical potential. The present study was intended to evaluate the impact of growing conditions on total phenolic and total flavonoid contents, and in vitro antioxidant potential in the bulbs and leaves of onion varieties planted under diverse environmental conditions. Standard analytical methods were used to quantify total phenolic content (TPC), total flavonoid content (TFC), and free radicals’ scavenging/antioxidant capacity. The impact of climatic and soil conditions was assessed using statistical tools. In general, onion varieties cultivated at three different locations viz. Kalar Kahar, Lahore and Swabi exhibited significant variations in TPC and TFC, and antioxidant activities. The bulbs and leaves of Mustang (V1) variety planted at Lahore and Swabi had significantly (p < 0.05), high levels of TPC (659.5 ± 6.59, and 631.1 ± 8.58 mg GAE/100 g, respectively). However, leaves of Red Orb (V2) and bulbs of Mustang (V1), and Golden Orb (V6), harvested from Kalar Kahar depicted the highest concentration of TFC (432.5 ± 10.3, 303.0 ± 6.67, and 303.0 ± 2.52 mg QE/100 g DW, respectively). Likewise, bulbs of V1 planted at Kalar Kahar, Lahore and Swabi exhibited maximum inhibition of DPPH, ABTS, and H2O2 radicals (79.01 ± 1.49, 65.38 ± 0.99, and 59.76 ± 0.90%, respectively). Golden Orb (V6) harvested from Lahore had the highest scavenging of OH radical (67.40 ± 0.09%). Likewise, bulbs of V1 variety planted at KalarKahar and Swabi had significant capacity to scavenge ferric ions (415.1 ± 10.6 mg GAE/100 g DW), and molybdate ions (213.7 ± 0.00 mg AAE/100 g DW). Conversely, leaves of Amazon (V8), planted at Lahore and Swabi depicted significant levels of DPPH, ABTS, H2O2 radical scavenging (90.69 ± 0.26, 63.55 ± 1.06, 51.86 ± 0.43%, respectively), and reduction of ferric ions (184.2 ± 6.75 mg GAE/100 g DW). V6 leaves harvested from Lahore and that of Super Sarhad (V3) from Swabi showed the highest inhibition of OH radical (61.21 ± 0.79%), and molybdate ions (623.6 ± 0.12 mg AAE/100 g DW), respectively. Pearson correlation and principal component analysis revealed strong relationships of climatic conditions, soil properties and elevation with TPC, TFC and free radicals’ scavenging potential in the bulbs and leaves of onion varieties. The variations in the total phenolic and flavonoid contents, and antioxidant potential of different varieties, and their associations with climatic and soil factors revealed the complexity of the growing conditions and genetic makeup that imposed significant impacts on the synthesis of secondary metabolites and nutraceutical potential of food and medicinal plant species.

For agricultural soils with low natural fertility, the possibility of using rock powders as an alternative source of nutrients and/or improver of soil physical parameters is under discussion and study. Shungite rocks, carbon-bearing volcanic sedimentary rock, are characterized by a high content of carbon and nutrients. This study aimed to evaluate whether shungite application to Umbric Podzols may affect leaf and root mitochondrial respiratory pathways, and the leaf response to temperature change. A pot culture experiment was conducted with Allium cepa L. seedlings, using soil shungite concentrations of 0, 5, 10, and 20 g kg−1 and two soil water regimes: well-watered (WW) and drying-wetting (DW) cycles. Soil water deficit increased total respiration (Vt) of onion leaves, but not roots, under low (13 °C) and high (33 °C) measurement temperature. Shungite application affected leaf Vt only at 13 °C: it increased the Vt rate under WW and decreased one under DW. An increase in the measurement temperature to 33 °C enhanced the sensitivity of leaf respiration to the inhibitor of the alternative respiratory pathway (salicylhydroxamic acid, SHAM). Shungite application increased the contribution of SHAM-sensitive pathway to the leaf Vt rate under WW, but not under the DW regime, regardless of the leaf temperature. In contrast to the SHAM-resistant pathway, the temperature sensitivity of the SHAM-sensitive rate decreased following the decrease in soil water availability. Shungite application increased the temperature sensitivity of both SHAM-sensitive and SHAM-resistant pathways under DW, and significantly decreased these parameters under WW. In summary, the decrease in temperature sensitivity of alternative SHAM-sensitive respiratory pathway with a decrease of soil water availability or shungite-related decrease of both SHAM-sensitive and SHAM-resistant leaf respiration may play an important role in enhancing the resistance of plant respiration to stress temperature.

Onion production is severely affected by waterlogging conditions, which are created due to heavy rainfall. Hence, the identification of waterlogging-tolerant onion genotypes is crucial for increasing onion production. In the present study, 100 distinct onion genotypes were screened for waterlogging tolerance under artificial conditions by using the phenotypic approach in the monsoon season of 2017. Based on plant survival and recovery and changes in bulb weight, we identified 19 tolerant, 27 intermediate tolerant, and 54 highly sensitive onion genotypes. The tolerant genotypes exhibited higher plant survival and better recovery and bulb size, whereas sensitive genotypes exhibited higher plant mortality, poor recovery, and small bulb size under waterlogging conditions. Furthermore, a subset of 12 contrasting genotypes was selected for field trials during monsoon seasons 2018 and 2019. Results revealed that considerable variation in the morphological, physiological, and yield characteristics were observed across the genotypes under stress conditions. Waterlogging-tolerant genotypes, namely, Acc. 1666, Acc. 1622, W-355, W-208, KH-M-2, and RGP-5, exhibited higher plant height, leaf number, leaf area, leaf length, chlorophyll content, membrane stability index (MSI), pyruvic acid, antioxidant content, and bulb yield than sensitive genotypes under stress conditions. Furthermore, the principal component analysis biplot revealed a strong association of leaf number, leaf area, chlorophyll content, MSI, and bulb yield with tolerant genotypes under stress conditions. The study indicates that the waterlogging-tolerant onion genotypes with promising stress-adaptive traits can be used in plant breeding programs for developing waterlogging-tolerant onion varieties.

This dataset has contributed to establish new reference values for organic matter and nitrogen content of twelve common arable crops in the Netherlands (see related publication). It contains crop residue data of dry- and organic matter and nitrogen content from two years of field sampling of above- and belowground plant residues. This research has been conducted with funding from the LNV-funded programme Slim Landgebruik, which is aimed at exploring the potential of carbon sequestration in agricultural soils. Two of the measures researched is the effect of crops in crop rotation and the incorporation of more crop residues into the soil. The dataset used for the related publication also contains literature data (see literature references), however due to copyright issues we have removed these entries from this published dataset.

Представлены результаты исследований влияния применения глауконитовых песков на основные биохимические показатели лука репчатого. Исследования проведены в 2020-2021 гг. в Федеральном научном центре овощеводства (Московская область) в открытом грунте и лабораторных условиях. Объект исследования – районированный сорт лука репчатого Мячковский. Использованы следующие дозы внесения глауконитовых песков: 150 г/м2, 300 г/м2, 450 г/м2, 600 г/м2 и контрольный образец без добавления глауконитовых песков. Максимальное содержание сухого вещества наблюдалось у сорта Мячковский с дозой внесения глауконитового песка 300 г/м2, Мячковский контроль и Мячковский 450 г/м2 соответственно 17,44; 17,41; 17,31. Наибольшее содержание витамина С было отмечено у Мячковский контроль и Мячковский 450 (8,8 мг%). Наибольший показатель суммы сахаров отмечен у Мячковский контроль, Мячковский 300 и Мячковский 450 соответственно 11,68; 12,24 и 11,68%. Выяснено, что при внесении глауконитовых песков значительно улучшаются биохимические показатели у сорта лука репчатого Мячковский, увеличивается процент сухого вещества. Оптимальные дозы внесения глауконитового песка 300 и 450 г/м2. Положительное влияние также оказывается и на массу луковицы., The results of studies of the effect of the use of glauconite sands on the main biochemical parameters of onions are presented. The research was carried out in 2020-2021 at the Federal Research Center for Vegetable Growing (the Moscow Region) in the open ground and laboratory conditions. The object of research is the variety of onion Myachkovsky. The following concentrations of glauconite sand were used: 150 g/m2, 300 g/m2, 450 g/m2, 600 g/m2 and a control sample without the addition of glauconite sands. The maximum dry matter content is observed in the samples Myachkovsky 300, Myachkovsky control, Myachkovsky 450 (17.44; 17.41; 17.31, respectively). The highest vitamin C content was observed in the Myachkovsky control and Myachkovsky 450 samples (8.8 mg%). The highest indicator is the sum of sugars in the Myachkovsky control sample, Myachkovsky 300 and Myachkovsky 450 (11.68; 12.24 and 11.68%, respectively). It was found out that when applying glauconite sands, the biochemical parameters of the onion variety Myachkovsky significantly improve, the content (%) of dry matter increases. The most suitable application concentrations are 300 and 450 g/m2. It also has a positive effect on the weight of the bulb.

Two field experiments were carried out at the village ten, Abu Humus, El-Beheira Governorate, Egypt, during the two successive seasons 2016/2017 and 2017/2018, to evaluate the effect of three types of herbicide and three irrigation intervals on productivity and quality of onion cultivar (Giza Red). The experiments were designed as split plot in randomized complete block design with three replications. Irrigation intervals – (I1) 20 days, (I2) 30 days, and (I3) 40 days – were assigned to the main plots. Herbicides treatments – (H1) Control, (H2) Fluazifop-p, (H3) Oxyfluorfen, and (H4) Clethodium – were distributed randomly in sub-plots. The results revealed that the interaction between herbicide and irrigation intervals had significant effects on productivity and quality of onion (Giza Red) cultivar in both seasons. Maximum onion bulbs yield (14.06 and 14.29 ton/fed) was recorded at (I3 with H3) treatments in first and second seasons, respectively. (I2 with H3) led to increase onion harvest index values. The highest value of carbohydrates percentage and total soluble solids (TSS) obtained at {I3 and H2}. Herbicides residues in dry onions bulbs under all treatments were found well below the maximum residue limit set by World Health Organization (WHO)/Food and Agriculture Organization (FAO) (0.05 mg/kg). In conclusion, the interaction of Oxyfluorfen herbicide and irrigation interval 40 days could be used to improve fresh and dry bulb yield (ton/fed) of onion cultivar, harvest index and water use efficiency (WUE). The treatment of {I3 (40 days) with H2 (Fluazifop-p)} could be used to enhance bulb diameter (cm) after harvest, TSS and the percentage of carbohydrates.

В статье представлены результаты селекционной работы по луку шалоту в различных селекционных питомниках. Работа была проведена в условиях Московской области. Из 80 образцов коллекционного питомника методом клонового отбора было выделено 8 перспективных образцов. Из данных номеров был отобран образец №39, который по своим показателям превзошёл стандарт. В 2018 году данный образец был передан В ГСИ и в 2020 году районирован как сорт Дачная соната., The article presents the results of selection work on shallots in various breeding nurseries. The work was carried out in the conditions of the Moscow region. Out of 80 samples of the collection nursery, 8 promising samples were selected by the clone selection method. Sample No. 39 was selected from these numbers, which surpassed the standard in its indicators. In 2018, this sample was transferred to the GSI and in 2020 it was zoned as a Dacha Sonata variety.

The enormous environmental problems that arise from organic waste have increased due to the significant population increase worldwide. Microbial fuel cells provide a novel solution for the use of waste as fuel for electricity generation. In this investigation, onion waste was used, and managed to generate maximum peaks of 4.459 ± 0.0608 mA and 0.991 ± 0.02 V of current and voltage, respectively. The conductivity values increased rapidly to 179,987 ± 2859 mS/cm, while the optimal pH in which the most significant current was generated was 6968 ± 0.286, and the ° Brix values decreased rapidly due to the degradation of organic matter. The microbial fuel cells showed a low internal resistance (154,389 ± 5228 Ω), with a power density of 595.69 ± 15.05 mW/cm2 at a current density of 6.02 A/cm2; these values are higher than those reported by other authors in the literature. The diffractogram spectra of the onion debris from FTIR show a decrease in the most intense peaks, compared to the initial ones with the final ones. It was possible to identify the species Pseudomona eruginosa, Acinetobacter bereziniae, Stenotrophomonas maltophilia, and Yarrowia lipolytica adhered to the anode electrode at the end of the monitoring using the molecular technique.

The study was conducted to test whether a vigor test of a single count of radicle emergence (RE test) would correlate with the storage potential of 10 seed lots of onion (Allium cepa L.) cultivars in artificial storage conditions. The RE test was performed by counting radicle emergence (2 mm) percentage at 80 h after germination was set up at 20 °C. Seed longevity was determined by storage at 75% relative humidity, obtained by saturated NaCl, at 35 °C over 60 days. Twelve seed samples were taken out during ageing, and seed survival curves were constructed based on normal germination percentages conducted at 20 °C for 12 days in the dark. The seed longevity criterion was P50 (time for the viability to fall to 50%), which was determined through probit analysis by using survival curves. Correlation analysis showed that RE counts at 80 h during germination were highly correlated (p < 0.001) with initial seed quality, Ki (R2 = 0.888) and the half-viability period, P50 (R2 = 0.823). Non-aged pre-storage final normal germination percentages of seed lots were also significantly related to longevity (Ki, R2 = 0.808, p < 0.001, P50, R2 = 0.750, p

Rising labor costs and the inefficient manual methods of cultivating red onions in Bangladesh contribute to the country’s failure to meet is own demand. Mechanizing the process of transplanting red onion seedlings will reduce the manual labor required while increasing crop yields. This paper provides an initial study of a proposed mechanized onion transplanter designed to attach to the back side of a two-wheel tractor with power tiller operated-seeder, commonly used in Bangladesh. Testing of a prototype made from these designs proves that the design is functional but requires further development for commercial/widespread use.

Introducing summer staple crops to diversify conventional summer paddy rice (Oryza sativa L.) and onion (Allium cepa L.) rotation is important for sustainable agriculture. Herein, we evaluate the effects of planting date (early June to late July) and tillage practice (deep cultivation, DC; conventional tillage, CT) on two maize and soybean cultivars over 4 years (2018–2021) in converted paddy soil in Korea. Due to the growing degree-day differences, the yields of June and July planted crops were 7050 and 5554 total digestible nutrient kg ha−1, respectively, for silage corn, and 7410 and 6473 marketable fresh ear kg ha−1, respectively, for waxy corn. Delaying soybean planting from June to July significantly reduced crops’ yield and interfered with field preparation for the following winter cash crops. The June and July planting yields were 2672 and 2090 kg ha−1, respectively, for large-grain soybean, and 2416 and 1861 kg ha−1, respectively, for small-grain soybean. Deep tillage had no effect on summer crops yield. Additionally, the summer crop growing practice had no residual effect on onion yield. Our study recommends mid to late June planting for growers who wish to introduce maize and soybean in rotation with winter onion in the region.

Verticillium wilt, caused by Verticillium dahliae, is the most devastating soil-borne fungal disease of olive trees worldwide. Currently, there is no effective measure available to control the pathogen in diseased plants in open field conditions. Searching more effective and sustainable solutions are a priority for the olive sector. The existing alternatives for disease control include the use of biological control microorganisms and compounds of natural origin from plants, such as Alliaceae. Propyl propane thiosulfinate (PTS) and propyl propane thiosulfonate (PTSO) are two organosulfur compounds derived from Allium cepa with a widely documented antimicrobial activity. The aim of this study was to evaluate the antifungal activity of PTS and PTSO against the defoliating and non-defoliating V. dahliae pathotypes. Firstly, several in vitro tests were performed (Minimum Antifungal Concentration, susceptibility studies according to the Kirby–Bauer disk-diffusion method, antifungal activity through aerial diffusion and effect on mycelial growth). The ability of both compounds to sanitize soil was evaluated using a sterile substrate inoculated with V. dahliae. Finally, challenges in growth chambers were carried out. PTS and PTSO generated growth inhibition zones in agar diffusion and the gas phase, and the mycelial growth of all the V. dahliae strains was significantly altered. The V. dahliae population in soil was considerably reduced after the sanitization. Finally, in planta assays demonstrated the ability of these compounds to reduce disease related parameters and their contribution to control the phytopathogen. In conclusion, the results showed that the PTS and PTSO from Allium cepa display in vitro and in vivo antifungal activity against V. dahliae and suggested that both compounds could be used as natural and environmentally friendly tools for Verticillium wilt management.

F1 populations of half diallel cross were considered for this investigation. Phenotypic component of variation (s2P) was higher than genotypic (s2G), interaction ((s2I) and within error (s2w) components of variation. Trait bulb weight showed the highest values for s2P, s2G, s2I and s2w. The noticeable amount of phenotypic, genotypic, interaction and within error covariation was found in the combination of BW×BY, possibly indicating wide scope of selection for this pair of characters. Genotypic correlations were higher than the respective phenotypic correlations. This situation was also marked in the path coefficient analysis. Bulb yield/plot showed highly significant and positive correlation coefficient with other characters both at phenotypic and genotypic levels. When all the nine characters were included in an index, it exhibited the highest genetic gain as percentage. When a combination of two or more characters was studied in a function, the efficacy was higher than that of direct selection for bulb yield. The combination of five, six, seven or eight characters showed higher percentage of expected gain. Due to significantly correlated with BY and having high positive direct effect at phenotypic level characters viz., LL, BW, PH and NLs is considered as primary yield components. Again combinations of these four characters gave the commendable expected genetic gain of 330.7290% may be considered as important selection index for this material.

Background oxidative stress and inflammation remain the main health problems often linked to degenerative diseases. The aim of this study was to evaluate antioxidant, analgesic and anti-inflammatory properties of onion growth in Cameroon. Methods Aqueous extracts were produced from two varieties of onion (Violet of galmi; Goudami) and were quantified for their polyphenols content. Concentrations of extracts (0.1, 0.5, 1, 1.5 mg/mL) were also prepared, their in vitro antioxidant properties evaluated by using standard methods. Anti-inflammatory effect of onion extracts was assessed by applying carrageenan sub plantar test to Wistar rats, but acetic acid test was used to study analgesic effect. Results The polyphenols content and antioxidant activities of onion extracts changed significantly (p p p p Conclusion The present findings suggest that aqueous extract of violet of galmi and Goudami bulb onions have antioxidant activity and protective effects against inflammation and pain.

Meat lipids oxidation during digestion gives rise to a post-prandial oxidative stress condition, which negatively affects human health. Mediterranean Diet vegetables contain high amount of phenolic compounds, which potentially may reduce the oxidative phenomena during digestion. In vitro co-digestion of turkey meat with a typical Mediterranean Diet salad containing tomato, onion, black olives, extra-virgin olive oil (EVOO) and basil, dose-dependently reduced lipid peroxidation. Onion and EVOO were more effective in limiting oxidation than the other foods, resulting in negligible concentrations of lipid hydroperoxides after digestion. Specific phenolic classes dominated the phenolic profile of the different foods, such as flavonols and anthocyanins in onion, phenolic acids in tomato and basil, and tyrosol-derivatives in black olives and EVOO. The correlation between lipid peroxidation inhibition, phenolic constituents and antioxidant properties was evaluated by principal component analysis (PCA). Flavonols and anthocyanin were the major contributors to the bioactive response of vegetable foods.

Bu çalışmada, Türkiye’de yerel olarak yetiştirilen bazı soğan (Allium cepa L.) genotiplerinin çiçek tomurcuğu kültürü yoluyla haploidiye yatkınlıklarının belirlenmesi amaçlanmıştır. Araştırmada kallus oluşumu, kallusların bitkiye dönüşümü ve eksplantlardan doğrudan bitki gelişimi üzerine genotip etkisi önemli bulunmuştur. Çalışmada kalluslardan ve doğrudan bitki gelişimi yoluyla 39 adet bitki elde edilmiştir. Yapılan ploidi analizleri sonucu Şanlıurfa ili Siverek ilçesinden temin edilen 13 numaralı genotipten elde edilen bir adet bitkinin haploid olduğu tespit edilmiştir. Dolayısıyla, diğer genotiplerle karşılaştırıldığında, hem eksplantlarda kallus oluşumu hem de doğrudan bitki meydana getirme kabiliyetinin yüksek olduğu tespit edilen 12, 13 ve 5 numaralı genotiplerin, soğan ıslah programlarında haploid bitki elde etmede daha başarılı sonuçlar verebileceği düşünülmektedir.

The use of Allium species and their extracts has been known since immemorial times due to their health beneficial properties. It is known that functional properties of Allium genus come from the high content of bioactive compounds. The biological activity of Allium extracts will be conditioned by the type of Allium variety, agricultural conditions, and specific extraction process used since all these factors affect the content and the profile of bioactive compounds. Innovative extraction techniques in comparison to conventional processes could be considered as a promising tool to recover bioactive compounds from Allium spp. with antimicrobial and anti-inflammatory properties. Trying to fill the gap in the literature, this paper reviews the chemical composition, the effects of processing on the nutritional and bioactive composition of Allium species and their extracts. Moreover, the antimicrobial and anti-inflammatory effects, as well as the bioavailability of bioactive compounds of edible members from the Allium genus is discussed.

Water stress negatively impacts the physiology of plants, affecting their growth and development. It is considered among the most important environmental factors responsible for reduced crop production. In this regard, biofertilizers may be considered significant for their reparative properties to increase stress tolerance in crop plants. We studied the effects of water stress on the morphological and biochemical characteristics of onion plants with AMF (Funneliformis mosseae) and potassium silicate application. The water stress significantly affected all the studied traits, each with minimum recorded levels. Plants that received combined treatments of AMF and potassium silicate showed maximum percent increments in all the studied characteristics, e.g., plant height (156.7%), weight of bulb (416.8%), antioxidant activity (224.0%), membrane stability index (74.5%), relative water content (87.3%), and total soluble solids (63.71%). Therefore, the study demonstrated that all the investigated variables were affected negatively by water stress. However, bio-fortification of onion plants with silicates and AMF inoculation may be considered useful for functional food production, with numerous health promoting properties.

The effective management of agro-industry organic waste for developing high-commercial-value products is a promising facet of the circular economy. Annually, more than 550,000 tons of waste that is potentially rich in biologically active substances is generated worldwide while processing onions (Allium cepa L.). The antioxidant potential of red, yellow, and white onion husks was studied using FRAP, ORAC, chemiluminescence, and UPLC-ESI-Q-TOF-MS analysis methods. The extraction of phenolic compounds from onion husks was more effective when using an aqueous solution of 70% ethanol as compared with water. Ethanolic extract from red onion husks exhibited the highest TACORAC and TACFRAP values, averaging 2017.34 µmol-equiv. Trolox/g raw material and 2050.23 µmol-equiv. DQ/g raw material, respectively, while the white onion exhibited much lower levels of antioxidants. According to the chemiluminescence results, it was determined that the red and yellow onion husks contained antioxidants of three types of power, while white onion husks only contained medium and weak types. The highest content of flavonoids was found in red onion husks, averaging 1915.90 ± 9.92 µg-eq. DQ/g of raw material and 321.42 ± 2.61 µg-eq. DQ/g of raw material for ethanol and water, respectively, while yellow onion husks exhibited 544.06 ± 2.73 µg-eq. DQ/g of raw material and 89.41 ± 2.08 for ethanol and water, respectively. Quercetin and its glycosides were the most representative flavonoids, and a number of substances with different pharmacological and biological properties were also identified.

The semi-continuous hydrolysis of onion skin wastes (OSW) using subcritical water (SubW; working conditions: 105-180 ºC; 5 MPa; 2.5 mL/min) has been studied in this work. Liquids after hydrolysis showed a sharp increase in total organic carbon (>30 mg/g OSW) at temperatures above 145 ºC, when SubW began to promote the partial hydrolysis of the structural components of OSW. Among them, pectin was one of main components recovered (extraction yield up to 9% at 145 ºC), whereas cellulose was barely hydrolyzed in the range of temperatures studied. The composition of pectin demonstrated that SubW promoted the recovery of the valuable RG-I (21.1±1.1 mol %) regions compared to acid water extraction processes (10.8±0.9 mol %). The control of the hydrolysis conditions was found to be critical, since high temperatures and long hydrolysis times led to the formation of organic acids (acetic, formic and levulinic) and degradation products such as furfural (up to 0.8 mg/g OSW) from the C5 sugars. Finally, the High Heating Value (HHV) calculated for the solid residue obtained at 180 ºC (16.4±0.2 MJ/kg) indicated the potential use of this residue as a fuel, once the valuable compounds (phenolics and pectins) have been recovered., Agencia Estatal de Investigación (AEI) though projects PID2020-116716RJ-I00/ AEI / 10.13039/501100011033 and PID2019-104950RB-I00 / AEI / 10.13039/501100011033 and by the Junta de Castilla y León (JCyL) and the European Regional Development Fund (ERDF) through projects BU301P18 and BU050P20. Benito-Román post-doctoral contract was funded by JCyL and EDRF through project BU050P20 and by AEI through project PID2020-116716RJ-I00. E. Díaz de Cerio post-doctoral contract was funded by JCyL through project BU050P20. P. Alonso-Riaño predoctoral contract was funded by JCyL through ORDEN EDU/556/2019, de 5 de junio.

Pantoea ananatis is a gram-negative bacterium and the primary causal agent of center rot of onions in Georgia. Previous genomic studies identified two virulence gene clusters, HiVir and alt, associated with center rot. The HiVir gene cluster is required to induce necrosis on onion tissues via synthesis of a predicted small molecule toxin. The alt gene cluster aids in tolerance to thiosulfinates generated during onion tissue damage. Whole genome sequencing of other Pantoea species suggest that these gene clusters are present outside of P. ananatis. To assess the distribution of these gene clusters, two PCR primer sets were designed to detect the presence of HiVir and alt. Two hundred fifty-two strains of Pantoea spp. were phenotyped using the red onion scale necrosis (RSN) assay and were assayed using PCR for the presence of these virulence genes. A diverse panel of strains from three distinct culture collections comprised of 24 Pantoea species, 41 isolation sources, and 23 countries, collected from 1946-2019, were tested. There is a significant association between the alt PCR assay and Pantoea strains recovered from symptomatic onion (PP. ananatis strains but not among Pantoea spp., congeners. This may indicate a divergent HiVir cluster or different pathogenicity and virulence mechanisms. Last, we describe natural alt positive [RSN+/HiVir+/alt+] P. ananatis strains, which cause extensive bulb necrosis in a neck-to-bulb infection assay compared to alt negative [RSN+/HiVir+/alt-] P. ananatis strains. A combination of assays that include PCR of virulence genes [HiVir and alt] and an RSN assay can potentially aid in identification of onion-bulb-rotting pathogenic P. ananatis strains.

The onion basal rot disease is a worldwide threat caused by species of the genus Fusarium. Today, Israel’s control of this disease is limited to a four-year growth cycle and Metam sodium soil disinfection. Here, commercial chemical fungicides were evaluated as control treatments against two of the primary pathogens involved, F. oxysporum f. sp. cepae and F. Acutatum. Out of 10 fungicides tested on culture plates, 3, Prochloraz, Azoxystrobin + Tebuconazole, and Fludioxonil + Sedaxen, had strong inhibitory effects on mycelial growth and were selected and tested in seeds in vitro. The preparations were applied as a seed coating and tested in two commercial cultivars, Riverside (Orlando, white cv.) and Noam (red cv.). Prochloraz (0.3% w/w concentration), the most promising compound, was efficient in reducing the Noam cv. sprouts’ disease symptoms. This preparation had no harmful in situ-toxicity effect and did not influence the plants’ seed germination and early development. In Noam cv. potted 30-day-old sprouts, the Prochloraz treatment was able to reduce the harmful impact of F. oxysporum f. sp. cepae. on the seedlings’ wet biomass, but was not effective in the Riverside cv. or against the F. acutatum pathogen. This suggests that future protective strategies must include an effective protective suit tailored to each of the pathogen species involved and the onion cultivar. The methods presented in this work can be applied for rapidly scanning multiple compounds while gradually ruling out ineffective ones. Eventually, this screening will enable field testing of the highest potential fungicides that successfully pass the pot experiments.

Context Allium cepa L. (Liliaceae), known as onion, is consumed throughout the world. Onion and its derivatives including saponins, aglycones, quercetin, cepaenes, flavonoids, organosulfurs, and phenolic compounds, showed various pharmacological properties and therapeutic effects. Objective Anti-inflammatory, antioxidant, and immunomodulatory effects of A. cepa and its main constituents, along with the underlying molecular mechanisms are presented. Methods Databases including, Web of Knowledge, Medline/PubMed, Scopus, and Google Scholar were checked for articles published between 1996 and the end of July 2020, using the key-words Allium cepa, quercetin, anti-inflammatory, antioxidant and immunomodulatory. Results A. cepa and its constituents mainly quercetin showed anti-inflammatory effects mediated via reduction of total and differential WBC counts, inhibition of chemotaxis of polymorphonuclear leukocytes, COX, and LOX pathways and prevented formation of leukotrienes and thromboxanes, prostaglandin E2 (PGE2) as onVCAM-1, NF-κB, MARK,d STAT-1, JNK, p38 and osteoclastogenesis. A. cepa and its derivatives showed antioxidant effect by decreasing lipid peroxidation, NAD(P)H, MDA, NO, LPO and eNOS but enhancing antioxidants such as SOD, CAT, GSH, GPx, GSPO, TrxR, SDH, GST and GR activities and thiol level. Immunomodulatory effects of the plant and quercetin was also shown by reduction of Th2 cytokines, IL-4, IL-5, and IL-13 as well as IL-6, IL-8, IL-10, IL-1β and TNF-α and IgE levels, but increased CD4 cells, IFN-γ level and IFN-γ/IL4 ratio (Th1/Th2 balance). Conclusions The effect of onion and its constituents on oxidative stress, inflammatory and immune system were shown indicating their therapeutic value in treatment of various diseases associated with oxidative stress, inflammation, and immune-dysregulation.

Metabolic syndrome as a clustering disorder includes excess abdominal fat distribution, abnormal insulin and glucose metabolism, disturbed blood lipids, pro-inflammatory state, and hypertension. Regarding to the adverse effects of synthetic medicines, the identification of appropriate healthcare approaches, such as herbal medicines, with fewer side effects is more favorable. Allium cepa L. (onion) is a culinary and medicinal herb belonging to the family of Amaryllidaceae. Flavonoids such as quercetin and kaempferol, alk(en)yl cysteine sulfoxides including S-methyl cysteine sulfoxide and S-propyl cysteine sulfoxide, cycloalliin, thiosulfinates, and sulfides are main compounds existing in the plant. A. cepa and its pharmacologically active constituents display broad-spectrum activities including anti-oxidant, anti-inflammatory, lipid-modifying, anti-obesity, antihypertensive, and antidiabetic effects. Our objective in this review was to find out the role of A. cepa and its bioactive phytochemicals as cardiovascular protective agents in different metabolic syndrome risk factors, including hyperlipidemia, high blood glucose, obesity, and hypertension.

Pantoea ananatis is a significant plant pathogen that targets a number of important crops, a problem that is compounded by the absence of effective treatments to prevent its spread. Our identification of pantaphos as the key virulence factor in onion center rot suggests a variety of approaches that could be employed to address this significant plant disease., Pantoea ananatis is the primary cause of onion center rot. Genetic data suggest that a phosphonic acid natural product is required for pathogenesis; however, the nature of the molecule is unknown. Here, we show that P. ananatis produces at least three phosphonates, two of which were purified and structurally characterized. The first, designated pantaphos, was shown to be 2-(hydroxy[phosphono]methyl)maleate; the second, a probable biosynthetic precursor, was shown to be 2-(phosphonomethyl)maleate. Purified pantaphos is both necessary and sufficient for the hallmark lesions of onion center rot. Moreover, when tested against mustard seedlings, the phytotoxic activity of pantaphos was comparable to the widely used herbicides glyphosate and phosphinothricin. Pantaphos was also active against a variety of human cell lines but was significantly more toxic to glioblastoma cells. Pantaphos showed little activity when tested against a variety of bacteria and fungi.

The onion is one of the most cultivated and consumed vegetables, and is a very valuable source of antioxidant substances. Every onion variety is rich in flavonols-mainly quercetin derivatives which makes onions the main dietary source of these compounds. This study intends to develop an ultrasound-assisted extraction method (UAE), an economical, green, and efficient technique, that allows us to determine the quality of onion extracts in terms of flavonol composition and antioxidant activity. For this purpose, an ultrasound-assisted extraction method has been optimized to obtain extracts with a high amount of flavonols, as well as with a high antioxidant activity, not only separately using a Box-Behnken design, but also simultaneously, based on multi-response optimization. Multiple response optimization has not only demonstrated an efficacy level similar to that of the individual ones, but it also represents a considerable reduction in terms of cost, time and work. The optimal conditions for simultaneous extractions were determined as follows: 76.8% methanol as an extraction solvent at pH 2 and 58.5 degrees C temperature, 85% amplitude, 0.9 s cycle, and 0.2:13 g:mL sample mass/solvent volume ratio. Furthermore, the developed method exhibited a high precision level and great recoveries in a rather short extraction time (2 min). These results, together with the development of a fast and simple UHPLC analysis method, lead us to consider the developed UAE method as a suitable technique for the extraction of bioactive compounds from onion matrices., This work has been supported by the project "EQC2018-005135-P" (Equipment for liquid chromatography by means of mass spectrometry and ion chromatography), of the State Subprogram of Research Infrastructures and Technical Scientific Equipment. This research was funded by University of Cadiz, by the INIA (National Institute for Agronomic Research) and FEDER (European Regional Development Fund, within the framework of the Operational Programme under the Investment for Growth 2014-2020) for their financial support (Project RTA2014-00083-C03-03), and by the Ministry of Science and Innovation of Spain (FPU grant AP-2018-03811 to Ana Velasco Gonzalez de Peredo).