1. Isolation and characterization of algicidal bacteria from Cochlodinium polykrikoides culture
- Author
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Sw Kim Si Wouk Kim, Jy Lee Ji-Young Lee, Mj Kim Min-Ju Kim, Ij Ko In-Jeong Ko, Wonduck Kim, and Ji Oh Jeong-Il Oh
- Subjects
biology ,Biomedical Engineering ,Dinoflagellate ,Bioengineering ,Cochlodinium polykrikoides ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Algal bloom ,Sagittula ,Microbiology ,Algae ,Thalassobius ,Gymnodinium ,Bacteria ,Biotechnology - Abstract
In this study, we analyzed a bacterial community closely associated with Cochlodinium polykrikoides that caused harmful algal blooming in the sea. Filtration using a plankton mesh and percoll gradient centrifugation were performed to eliminate free-living bacteria. Attached bacteria were analyzed by culture-dependent and culture-independent methods. Five culturable bacterial strains were isolated and identified from the C. polykrikoides mixed bacterial community. The isolates belonged to α-Proteobacteria (Nautella sp., Sagittula sp., and Thalassobius sp.) and γ-Proteobacteria (Alteromonas sp. and Pseudoalteromonas sp.). All of the 5 isolates showed algicidal activity against C. polykrikoides and produced extracellular compounds responsible for algicidal properties after entering the stationary phase. The algicidal compounds produced by the 5 isolates were heat-stable and had molecular masses of less than 10,000 Da. Furthermore, the algicidal compounds were relatively specific for C. polykrikoides in terms of their algicidal activities. Culture-independent analysis of the bacterial community in association with C. polykrikoides was performed using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). On the basis of the PCR-DGGE profile, Sagittula sp. was identified as a dominant species in the bacterial community of C. polykrikoides.
- Published
- 2011