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Additional file 1: Table S1. Anaerobic growth (indicated by OD600 values) of nine probiotic strains in the presence of FOS and non-FOS sugars.

Additional file 1. Experiment method: Elemental analysis of molasses. Fig. S1. Effect of exogeneous nitrogen and phosphorous on cell growth (A) and sugar consumption (B). Total levels of N and P shown in Table 1. Fig. S2. Effect of exogeneous nitrogen and phosphorous on specific intracellular transfructosylating activity (A), intracellular (B), extracellular (C), and total (D) enzyme activities based on per unit of volume. Total levels of N and P shown in Table 2. Fig. S3. HPLC chromatographs of FOS samples from 1 h enzymatic hydrolysis using reactor enzymes. Fig. S4. HPLC chromatographs of FOS samples from 3 h enzymatic hydrolysis using reactor enzymes. Fig. S5. HPLC chromatographs of FOS samples from 12 h enzymatic hydrolysis using reactor enzymes

Sweet sorghum is receiving significant global interest as an agro-industrial crop because of its capacity to co-produce energy, food, and feed products in integrated biorefineries. This report assesses the opportunities to develop a sweet sorghum industry in Australia, reports on research demonstrating the production of energy, food, and feed products, and assesses the potential economic and sustainability benefits of sweet sorghum biorefineries in the Australian context.