1. Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells
- Author
-
Laura Giannotti, Benedetta Di Chiara Stanca, Paola Nitti, Francesco Spedicato, Fabrizio Damiano, Christian Demitri, Nadia Calabriso, Maria Annunziata Carluccio, Andrea Palermo, Franco Ferrante, Luisa Siculella, Eleonora Stanca, Giannotti, Laura, DI CHIARA STANCA, Benedetta, Nitti, Paola, Spedicato, Francesco, Damiano, Fabrizio, Demitri, Christian, Calabriso, Nadia, Annunziata Carluccio, Maria, Palermo, Andrea, Ferrante, Franco, Siculella, Luisa, and Stanca, Eleonora
- Subjects
CGF, blood-derived biomaterials, growth factors, hydroxyapatite silicon scaffold, osteogenic differentiation, tissue regeneration ,General Immunology and Microbiology ,blood-derived biomaterials ,CGF ,growth factors ,osteogenic differentiation ,hydroxyapatite–silicon scaffold ,tissue regeneration ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology - Abstract
Simple Summary: The aim of this study was to identify new and innovative strategies to improve the tissue-regeneration process. Concentrated growth factor (CGF) is an autologous biomaterial rich in growth factors and multipotent stem cells. The purpose of our study was to evaluate the osteogenic differentiation of CGF primary cells in the presence of a hydroxyapatite–silicon scaffold, which represents a very interesting material in the field of bone reconstructive surgery. Our findings showed that the hydroxyapatite–silicon scaffold provided support to primary CGF cells by enhancing osteogenic differentiation. These data suggest interesting perspectives in the use of CGF together with scaffolds in the field of regenerative medicine. Abstract: The application of scaffolding materials together with stem cell technologies plays a key role in tissue regeneration. Therefore, in this study, CGF (concentrated growth factor), which represents an autologous and biocompatible blood-derived product rich in growth factors and multipotent stem cells, was used together with a hydroxyapatite and silicon (HA-Si) scaffold, which represents a very interesting material in the field of bone reconstructive surgery. The aim of this work was to evaluate the potential osteogenic differentiation of CGF primary cells induced by HASi scaffolds. The cellular viability of CGF primary cells cultured on HA-Si scaffolds and their structural characterization were performed by MTT assay and SEM analysis, respectively. Moreover, the matrix mineralization of CGF primary cells on the HA-Si scaffold was evaluated through Alizarin red staining. The expression of osteogenic differentiation markers was investigated through mRNA quantification by real-time PCR. We found that the HA-Si scaffold was not cytotoxic for CGF primary cells, allowing their growth and proliferation. Furthermore, the HASi scaffold was able to induce increased levels of osteogenic markers, decreased levels of stemness markers in these cells, and the formation of a mineralized matrix. In conclusion, our results suggest that HA-Si scaffolds can be used as a biomaterial support for CGF application in the field of tissue regeneration.
- Published
- 2023