1. Development of compound microsatellite markers in the toxic dinoflagellate Alexandrium catenella (Dinophyceae)
- Author
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Masashi Sekino, Satoshi Nagai, Patrick Berrebi, Daniel Grzebyk, Estelle Masseret, C. L. Lian, Shigeru Itakura, Nina Yasuda, Sanae Yamaguchi, Goh Nishitani, Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea, National Research Institute of Fisheries and Environment, Ecosystèmes lagunaires : organisation biologique et fonctionnement (ECOLAG), Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS), Asian Natural Environmental Science Center, The University of Tokyo (UTokyo), Graduate School of Information Science and Engineering, Tokyo Institute of Technology [Tokyo] (TITECH), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Tohoku National Fisheries Research Institute, National Fisheries Research Institute, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,microsatellite ,Alexandrium catenella ,Aquatic Science ,Oceanography ,010603 evolutionary biology ,01 natural sciences ,law.invention ,law ,Botany ,14. Life underwater ,Allele ,Ecology, Evolution, Behavior and Systematics ,Polymerase chain reaction ,Genetics ,SSR markers ,Ecology ,biology ,010604 marine biology & hydrobiology ,Dinoflagellate ,biology.organism_classification ,[SDE.BE.GP]Environmental Sciences/Biodiversity and Ecology/domain_sde.be.gp ,Genetic marker ,Genetic structure ,phytoplankton ,Microsatellite ,toxic dinoflagellate ,Dinophyceae - Abstract
International audience; In this study, we identified 9 polymorphic compound microsatellite markers in the toxic dinoflagellate Alexandrium catenella, which was isolated from Thau lagoon (France, Mediterranean Sea), using the compound microsatellite marker technique. These new microsatellites were characterized by screening DNA templates from 43 A. catenella clonal strains, which were collected from a seawater sample from Inokushi Bay (Oita Prefecture, Japan). These loci provide one class of highly variable genetic marker: the number of alleles ranged from 3 to 8, and the estimate of gene diversity varied between 0.285 and 0.762. We also analyzed the same 43 DNA samples using microsatellite markers previously identified for A. catenella, comparing the PCR amplification success, the number of alleles and gene diversity. These three parameters were not significantly different, showing that the compound microsatellite markers have the same potential to reveal A. catenella genetic structure. This simple and efficient method reduces the costs for developing SSR markers and for analyzing the genetic structure of populations, therefore, suggesting the effectiveness of applying this method to other species.
- Published
- 2007