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Supplemental material, sj-docx-1-pmj-10.1177_02692163221133665 for ‘Sadly I think we are sort of still quite white, middle-class really’ – Inequities in access to bereavement support: Findings from a mixed methods study by Lucy E Selman, Eileen Sutton, Renata Medeiros Mirra, Tracey Stone, Emma Gilbert, Yansie Rolston, Karl Murray, Mirella Longo, Kathy Seddon, Alison Penny, Catriona R Mayland, Donna Wakefield, Anthony Byrne and Emily Harrop in Palliative Medicine

Supplemental material, sj-pdf-2-pmj-10.1177_02692163221133665 for ‘Sadly I think we are sort of still quite white, middle-class really’ – Inequities in access to bereavement support: Findings from a mixed methods study by Lucy E Selman, Eileen Sutton, Renata Medeiros Mirra, Tracey Stone, Emma Gilbert, Yansie Rolston, Karl Murray, Mirella Longo, Kathy Seddon, Alison Penny, Catriona R Mayland, Donna Wakefield, Anthony Byrne and Emily Harrop in Palliative Medicine

Supplemental material, sj-pdf-3-pmj-10.1177_02692163221133665 for ‘Sadly I think we are sort of still quite white, middle-class really’ – Inequities in access to bereavement support: Findings from a mixed methods study by Lucy E Selman, Eileen Sutton, Renata Medeiros Mirra, Tracey Stone, Emma Gilbert, Yansie Rolston, Karl Murray, Mirella Longo, Kathy Seddon, Alison Penny, Catriona R Mayland, Donna Wakefield, Anthony Byrne and Emily Harrop in Palliative Medicine

The CA1 region of the hippocampus plays a critical role in spatial and contextual memory, and has well-established circuitry, function and plasticity. In contrast, the properties of the flanking CA2 pyramidal neurons (PNs), important for social memory, and lacking CA1-like plasticity, remain relatively understudied. In particular, little is known regarding the expression of voltage-gated K+ (Kv) channels and the contribution of these channels to the distinct properties of intrinsic excitability, action potential (AP) waveform, firing patterns and neurotransmission between CA1 and CA2 PNs. In the present study, we used multiplex fluorescence immunolabeling of mouse brain sections, and whole-cell recordings in acute mouse brain slices, to define the role of heterogeneous expression of Kv2 family Kv channels in CA1 versus CA2 pyramidal cell excitability. Our results show that the somatodendritic delayed rectifier Kv channel subunits Kv2.1, Kv2.2, and their auxiliary subunit AMIGO-1 have region-specific differences in expression in PNs, with the highest expression levels in CA1, a sharp decrease at the CA1-CA2 boundary, and significantly reduced levels in CA2 neurons. PNs in CA1 exhibit a robust contribution of Guangxitoxin-1E-sensitive Kv2-based delayed rectifier current to AP shape and after-hyperpolarization potential (AHP) relative to that seen in CA2 PNs. Our results indicate that robust Kv2 channel expression confers a distinct pattern of intrinsic excitability to CA1 PNs, potentially contributing to their different roles in hippocampal network function.

Figure S1. Expression vector maps used to generate the (A) EGFP-CGG99-EGFP or (B) EGFP-CGG11-EGFP transgenic mouse lines. Figure S2. Statistical results for behavioral experiments. (DOCX 339 kb)

Figure S1. Expression vector maps used to generate the (A) EGFP-CGG99-EGFP or (B) EGFP-CGG11-EGFP transgenic mouse lines. Figure S2. Statistical results for behavioral experiments. (DOCX 339 kb)