1. Expression and DNA microarray analysis of a platelet activating factor-related molecule in severe pneumonia in mice due to influenza virus and bacterial co-infection
- Author
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Seki, M., Kosai, K., Hara, A., Imamura, Y., Nakamura, S., Kurihara, S., Izumikawa, K., Kakeya, H., Yamamoto, Y., Yanagihara, K., yoshitsugu miyazaki, Mukae, H., Tashiro, T., and Kohmo, S.
- Subjects
Microbiology (medical) ,Male ,Blotting, Western ,General Medicine ,Platelet Membrane Glycoproteins ,Pneumonia ,Reverse Transcription ,respiratory system ,Microarray Analysis ,Polymerase Chain Reaction ,respiratory tract diseases ,Receptors, G-Protein-Coupled ,Mice ,Infectious Diseases ,Influenza A Virus, H1N1 Subtype ,Streptococcus pneumoniae ,Orthomyxoviridae Infections ,1-Alkyl-2-acetylglycerophosphocholine Esterase ,Mice, Inbred CBA ,Animals ,lipids (amino acids, peptides, and proteins) ,RNA, Messenger ,Platelet Activating Factor - Abstract
Platelet-activating factor (PAF) is a critical mediator of severe inflammatory diseases such as pneumonia, and the PAF-receptor (PAFR) is known to be an anchor for Streptococcus pneumoniae attachment to lung epithelial cells. We conducted a DNA microarray analysis to detect critical factors that mediate fulminant pneumonia due to influenza virus and S. pneumoniae co-infection in mice. Among the factors detected, levels of PAF-acetyl hydrolase (PAF-AH), which functions as inactivated PAF, were significantly increased, and PAFR was expressed in co-infected mouse lungs, as compared to the respective levels in mice infected with either S. pneumoniae or virus alone. Significantly elevated PAF-AH enzymatic activity was observed in the co-infected mouse lung, suggesting that co-infection activated PAF-related factors. These findings suggest that PAF and related molecules play important roles in fulminant pneumonia due to influenza virus infection, especially when severe bacterial pneumonia is complicated by co-infection with influenza virus., Japanese Journal of Infectious Diseases, 62(1), pp.6-10; 2009
- Published
- 2009