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Additional file 3. Gviz plots of significant DMRs between vitamin-C supplemented smokers and placebo. All 93 DMRs are plotted and arranged in alphabetical order based on the nearest annotated gene. Each page represents a single DMR and is organized into multiple tracks showing genomic ranges, CpG island locations, DMR location, and CpG level data tracks for each comparison (placebo vs non-smoker and vitamin C vs placebo).

Additional file 1. Figures: Figure S1. Flowchart of the analysis steps; Figure S2. Consort diagram of samples used in analysis; Figure S3. QQ-plot for unadjusted vs adjusted models; Figure S4. Comparison of FDR significant smoking DMCs with previous studies; Figure S5. Top eQTMs associated with vitamin C vs placebo DMRs; Figure S6. Top DMCs associated with m12 FEF75; Figure S7. Heatmap of FDR DMCs associated with m12 FEF75; Figure S8. Visual summary of DIP2C findings. Supplemental Methods.

A high-throughput liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed for the determination of paraquat and diquat in potatoes using a reversed-phase with weak anion-exchange and cation-exchange mixed-mode Acclaim™ Trinity Q1 column. Five grams of sample was shaken with fifteen milliliters of 1:1 MeOH: 0.1 N HCl for 10 min at room temperature and heated for 15 min at 80°C. After centrifugation, the supernatant was passed through an Oasis HLB SPE to retain suspended particulates and non-polar interferences. The sample was directly injected and analyzed for 10 min by LC-MS/MS with no sample concentration. Two multiple reaction monitoring (MRM) channels were monitored in the method for each target compound to achieve true positive identification. Two internal standards corresponding to each analyte were used to counter matrix suppression effect. Linearity of the detector response with a minimum coefficient of determination (r2) of more than 0.997 was demonstrated in the range from 2 to 250 ng/mL for each analyte using matrix-matched standard. The average recovery for all analytes at 10, 25, 100, and 500 ng/g (n$=$5) are between 87-106% with a relative standard deviation of less than 12%.https://doi.org/10.21423/jrs-v05n01p001 (DOI assigned 4/17/2019)

A simple high-throughput liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed for the determination of 2,4-dichlorophenoxy acetic acid (2,4-D) in soybean and corn using a reversed-phase with weak anion-exchange and cation-exchange mixed-mode Acclaim™ Trinity™ Q1 column. The method involved an alkaline hydrolysis with sodium hydroxide in order to convert all forms of 2,4-D into a salt form prior to extraction with acidified acetonitrile. The acetonitrile was salted out of the extract with sodium chloride and magnesium sulfate. The acetonitrile extract was diluted 1:1 with water and filtered before analysis by LC-MS/MS in a negative mode. Recoveries were evaluated at 10, 100, and 500 ng/g with seven replicates. Mean recovery ranged from 86 to 107% with relative standard deviation of less than 10%. Matrix suppression was not observed in this procedure; therefore, no internal standard was needed. The method was used successfully for the determination of 2,4-D in soybean and corn samples containing incurred residue. The procedure proved to be quick, accurate, precise, sensitive, and selective.https://doi.org/10.21423/jrs-v04n02p009 (DOI assigned 7/3/2019)

A simple high-throughput liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed for the determination of glyphosate, aminomethylphosphonic acid (AMPA) and glufosinate in milk using a reversed-phase liquid chromatography column with weak anion/cation exchange stationary phase. One milliliter of milk was shaken with three milliliters of water containing Na2EDTA and acetic acid for 10 minutes, to precipitate the protein content in the sample. After centrifugation, the supernatant was passed through an Oasis HLB SPE to retain suspended particulates and phospholipids. The sample was directly injected and analyzed in 6 min, with no sample concentration or derivatization steps. Two multiple reaction monitoring (MRM) channels were monitored in the method for each target compound to achieve true positive identification. Three internal standards corresponding to each analyte were used to counter matrix suppression effects. The linearity of the detector response was demonstrated in the range of 4 to 1000 ng/mL for each analyte, with a minimum coefficient of determination (R2) value of more than 0.995. Through the use of this internal standard calibration method, the average recovery for all analytes at 0.025, 0.1, 0.5, and 2 µg/g (n = 7) are between 84-111% with a relative standard deviation of less than 8%.https://doi.org/10.21423/jrs-v03n02p020 (DOI assigned 8/7/2019)

OBJECTIVESThe purpose of this study was to determine long-term outcome in adults with congenitally corrected transposition of the great arteries (CCTGA), with particular emphasis on systemic ventricular dysfunction and congestive heart failure (CHF).BACKGROUNDPatients with CCTGA have the anatomical right ventricle as their systemic pumping chamber, with ventricular dysfunction and CHF being relatively common in older adults.METHODSRetrospective analysis of records of 182 patients from 19 institutions were reviewed to determine current status and possible risk factors for systemic ventricular dysfunction and CHF. Factors considered included age, gender, associated cardiac defects, operative history, heart block, arrhythmias and tricuspid (i.e., systemic atrioventricular) regurgitation (TR).RESULTSBoth CHF and systemic ventricular dysfunction were common in groups with or without associated cardiac lesions. By age 45, 67% of patients with associated lesions had CHF, and 25% of patients without associated lesions had this complication. The rates of systemic ventricular dysfunction and CHF were higher with increasing age, the presence of significant associated cardiac lesions, history of arrhythmia, pacemaker implantation, prior surgery of any type, and particularly with tricuspid valvuloplasty or replacement. Aortic regurgitation (a previously unreported problem) was also relatively common in this patient population.CONCLUSIONSPatients with CCTGA are increasingly subject to CHF with advancing age; this complication is extremely common by the fourth and fifth decades. Tricuspid (systemic atrioventricular) valvular regurgitation is strongly associated with RV (anatomical right ventricle connected to aorta in CCTGA patients; systemic ventricle in CCTGA) dysfunction and CHF; whether it is causative or a secondary complication remains speculative.