1. Gene transfer into intact plant cells by electroinjection through cell walls and membranes
- Author
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Iida Asako, Matsui Chiaki, Morikawa Hiromichi, Ikegami Masato, and Yamada Yasuyuki
- Subjects
viruses ,Nicotiana tabacum ,Fluorescent Antibody Technique ,Virus ,Capsid ,Tobacco ,Genetics ,Tobacco mosaic virus ,Cloning, Molecular ,Cells, Cultured ,Infectivity ,biology ,fungi ,Genetic transfer ,food and beverages ,Tobamovirus ,General Medicine ,Plants ,biology.organism_classification ,Plant cell ,Molecular biology ,Tobacco Mosaic Virus ,Plants, Toxic ,Cell culture ,RNA, Viral ,Genetic Engineering - Abstract
Tobacco mosaic virus (TMV) RNA was introduced directly into mesophyll cells of Nicotiana tabacum var. Samsun using electric-field pulses (electroinjection). The injected gene was successfully expressed in the recipient cells as judged by the assay for the virus coat protein using immunofluorescence and by the virus infectivity assay of the homogenate of the electroinjected cells for local lesions on tobacco leaves. As much as 50% of the cells that survived 24 days after electroinjection showed immunofluorescent specks.
- Published
- 1986