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Example weeks from dataset 1 and dataset 2 post-alignment. Sample weeks shown here are to serve as a visual representation of the method outlined to evaluate age distribution. Dataset 1 (left) and dataset 2 (right) were combined and aligned following the selection of “epidemic weeks”. A week in which influenza A or B cases accounted for more than 90 % of all positive influenza cases was defined as an epidemic week. In the year 2007, you see an example of a defined influenza A epidemic week, whereas the year 2011 is a representative influenza B epidemic week. The week from 2013 displays a typical week which was removed from our age distribution analysis because neither influenza A nor B was dominant (>90 %). (PPTX 66 kb)

Example weeks from dataset 1 and dataset 2 post-alignment. Sample weeks shown here are to serve as a visual representation of the method outlined to evaluate age distribution. Dataset 1 (left) and dataset 2 (right) were combined and aligned following the selection of “epidemic weeks”. A week in which influenza A or B cases accounted for more than 90 % of all positive influenza cases was defined as an epidemic week. In the year 2007, you see an example of a defined influenza A epidemic week, whereas the year 2011 is a representative influenza B epidemic week. The week from 2013 displays a typical week which was removed from our age distribution analysis because neither influenza A nor B was dominant (>90 %). (PPTX 66 kb)

The aim of this study was to determine the optimal temperature of graft preservation after ex vivo gene transfer to rat lung isografts. Left lungs were harvested and infused with cationic lipid/LacZ-DNA complex via the pulmonary artery, and the grafts were stored for 4h. The grafts (n＝7) were allocated into groups IﾝIV according to the storage temperature:4℃, 10℃, 16℃, and 23℃, respectively. Forty-eight h after orthotopic transplantation, the arterial blood gas was analyzed and the peak airway pressure (PAP) and the level of LacZ protein production in the grafts were measured by reverse transcription polymerase chain reaction. After reperfusion, the grafts were stained with hematoxylin and eosin. The grafts in groups III and IV showed more deterioration as evidenced by decreased arterial oxygen tension, increased PAP, and predominant infiltration of inflammatory cells compared with groups I and II. The level of LacZ production was significantly lower in group I than in groups IIﾝIV. The optimal temperature of lung graft preservation after ex vivo gene transfer was determined to be 10℃, balancing considerations of lung injury and efficiency of transgene expression.