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Resumen del poster presentado en: I Spanish-Portuguese Congress on Beneficial Plant-Microbe Interactions (BeMiPlant) and XVIII National Meeting of the Spanish Society of Nitrogen Fixation (XVIII SEFIN). Oeiras, Portugal, 17-19 octubre (2022), This work was supported by grants BFU2017-82645-P and PID2020-114782GB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe” (BFU2017-82645-P).

Resumen del poster presentado en: I Spanish-Portuguese Congress on Beneficial Plant-Microbe Interactions (BeMiPlant) and XVIII National Meeting of the Spanish Society of Nitrogen Fixation (XVIII SEFIN). Oeiras, Portugal, 17-19 octubre (2022), This work was supported by grants BFU2017-82645-P and PID2020-114782GB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe” (BFU2017-82645-P).

Resumen de la comunicación oral presentada en: XIII Reunión del Grupo de Microbiología Molecular de La SEM. Granada, 7-9 septiembre (2022), This work was supported by grants BFU2017-82645-P and PID2020-114782GB-I00 funded by MCIN/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”.

Trabajo presentado en XVII National Meeting of the Spanish Society of Nitrogen Fixation - VI Portuguese-Spanish Congress on Nitrogen Fixation, celebrado en Madrid (España) del 10 al 12 de julio de 2019., Small non-coding RNAs (sRNAs) are ubiquitous components of regulatory netwoks underlying virtually any adaptive response in bacteria. As regulators of gene expression, sRNAs accumulate differentially in the cell in response to external cues and their main activity mechanism involves protein-assisted base-pairing interactions with short and discontinuous stretches of complementary nucleotides usually mapping to the translation initiation region of multiple trans-encoded target mRNAs. In most cases, this antisense interaction promotes post-transcriptional gene silencing by blocking of translation and degradation of the target mRNAs by cellular RNases (reviewed in Jiménez-Zurdo et al. 2013). In our laboratory, we are interested in the characterization of the non-coding transcriptome of the alfalfa symbiont Sinorhizobium meliloti. Deletion of one of the hundreds of sRNA genes identified in this bacterium alters nodule organogenesis and compromises nitrogen-fixation efficiency on alfalfa roots (Robledo et al, 2017). According to this symbiotic phenotype, this sRNA was named NfeR1 (Nodule Formation Efficiency RNA). Computational predictions, some of which have been verified experimentally, suggested that NfeR1 uses redundantly three identical anti-Shine Dalgarno (aSD) motifs for targeting and down-regulation of multiple mRNAs coding for the periplasmic component of ABC transport systems. Northern blot probing and transcription tracking with promoter-eGFP fusions uncovered maximum NfeR1 accumulation upon an osmotic upshift in free-living bacteria and during all steps of the symbiotic interaction. The strength and differential regulation of nfeR1 transcription is conferred by a motif that is conserved in the promoter regions of its ¿-proteobacterial homologs. Pull-down assays revealed that the NfeR1 promoter might bind the global nitrogen regulator NtrC and the LysR type transcriptional regulator LsrB (LysR Symbiotic Regulator). In the communication, we will provide novel insights into the transcriptional regulation, targeting potential, activity mechanism and symbiotic function of NfeR1., Funded by MINECO Feder/EU BFU2013-48282-C2-2-P and MINEICO Feder/EU BFU2017-82645-P

Este artículo reporta los resultados de una investigación en el área de la educación en Chile que tuvo por objeto analizar el grado de respuesta del desempeño académico de alumnos de escasos recursos a programas de reforzamiento educacional. La conclusión principal del estudio es que el rendimiento de estos alumnos (medido como puntajes en la Prueba de Aptitud Académica, (PAA) es altamente sensible a programas de entrenamiento y reforzamiento académico, incluso en períodos breves. En un programa diseñado para estos efectos, los alumnos participantes en promedio lograron elevar sus puntajes en la PAA de Matemáticas respecto de sus pares (un grupo de control) en 40 puntos, tras un período de entrenamiento de sólo cuatro meses. Esta cifra aumentaba a 96 puntos en aquellos alumnos con una asistencia superior o igual al 80 por ciento de las clases impartidas. Este importante resultado señala que, con esfuerzo y métodos adecuados, es posible generar avances significativos en materia de educación en períodos breves, a pesar de las deficiencias educacionales y factores socioeconómicos que frecuentemente circundan a esta población estudiantil. Por lo tanto, esta investigación provee evidencia sobre una forma probada de reducir las inequidades en el acceso de alumnos de escasos recursos a la educación superior, además de plantear algunas implicaciones para el diseño de políticas en el área de la educación media y superior This article reports the results of an experiment aiming to investigate the effect of training on the performance of poor students in PAA, a compulsory multiple-choice test required to enroll in university education in Chile. The main conclusion is that poor students´ performance in highly responsive to training, even in a short span of time. The trained students increased their scores in 40 points in only four months relative to a proper control group. This difference raises up to 96 points for those attending over 80 percent of the sessions. This suggests that by means of appropriate effort and training methods, important achievements can be obtained in the short run in poor students´ academic performance and future educational prospects, despite their adverse economic and social background. These findings suggest some policy implications for public policy in the areas of education, and it raises some questions for future research