1. Influence of perfusate temperature on nasal potential difference
- Author
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Bronsveld, Inez, Vermeulen, François, Sands, Dorotha, Leal, Teresinha, Leonard, Anissa, Melotti, Paola, Yaakov, Yasmin, de Nooijer, Roel, De Boeck, Kris, Sermet, Isabelle, Wilschanski, Michael, Middleton, Peter G, European Cystic Fibrosis Society – Diagnostic Network Working Group, UCL - SSS/IREC/LTAP - Louvain Centre for Toxicology and Applied Pharmacology, UCL - (SLuc) Service de pneumologie, and UCL - (SLuc) Service de biochimie médicale
- Subjects
Adult ,Pulmonary and Respiratory Medicine ,Time Factors ,Adolescent ,Cystic Fibrosis ,Analytical chemistry ,Amiloride ,Young Adult ,Chlorides ,Chloride Channels ,Isoprenaline ,Healthy volunteers ,medicine ,Humans ,Ions ,Ion Transport ,business.industry ,Isoproterenol ,Temperature ,Outcome measures ,Middle Aged ,Healthy Volunteers ,Perfusion ,Nasal Mucosa ,Potential difference ,Anesthesia ,Room temperature decreased ,business ,medicine.drug - Abstract
Nasal potential difference (NPD) quantifies abnormal ion transport in cystic fibrosis. It has gained acceptance as an outcome measure for the investigation of new therapies. To quantify the effect of solution temperature on NPD, we first examined the effect of switching from room temperature (20-25°C) to warmed (32-37°C) solutions and vice versa during each perfusion step. Secondly, standard protocols were repeated at both temperatures in the same subjects. Changing solution temperature did not alter NPD during perfusion with Ringer's solution (0.1). During perfusion with zero chloride solution, changing from room temperature to warmed solutions tended to decrease absolute NPD (i.e. it became less negative) by 0.9 mV (p>0.1); changing from warmed to room temperature increased NPD by 2.1 mV (p
- Published
- 2012
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