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Microalgae are a source material in food, pharmacy, and cosmetics industries for producing various products including high-protein nutritional supplements, synthetic pharmaceuticals, and natural colors. A promising algal source for such productions is Chlorella vulgaris which contains a considerable protein content. Similar to other microalgae, its desirability is minimal nutrient requirements since they are unicellular, photosynthetic, and fast-growing microorganisms. Another propitious option to be produced by C. vulgaris is biodiesel, since it is rich in oil too. Besides, algal well thriving in presence of increased amount of carbon dioxide makes them a practicable alternative biofuel resource without some problems of the traditional ones. At the same time, C. vulgaris is also a promising source for nutraceuticals such as amino acids, vitamins, and antioxidants. This review aims to discuss the conditions need to be observed for achieving a favorable growth efficiency of the C. vulgaris, as well as targeted productions such as biomass, antioxidant, and biofuel. Additionally, different approaches to induce any specific production are also considered comprehensively.

This study explores the production of prebiotic xylooligosaccharide (XOS) from cassava pulp waste and its effectiveness for the growth of Lactobacillus acidophilus (L. acidophilus). We successfully produced and characterized XOS from cassava pulp xylan using a Bacillus sp. endo-β-1,4-D-xylanase. The XOS was added to modify the MRS medium (MRSm) in various concentrations (0, 1, 3 and 5%) in which the L. acidophilus was inoculated. The growth of L. acidophilus was observed every 12 h for 2 days, and the fermentation products were analyzed for pH, sugar content, and short-chain fatty acids (SCFA) in terms of types and amount. The study showed that L. acidophilus grew well in MRSm. The optimum XOS concentration in MRSm was 5%, indicated by the highest growth of L. acidophilus (8.61 log CFU mL−1). The profile of SCFA products is 14.42 mM acetic acid, 0.25 mM propionic acid, 0.13 mM isobutyric acid, 0.41 mM n-butyric acid, 0.02 mM n-valeric acid, 0.25 mM isovaleric acid, and 25.08 mM lactic acid.

Асякина Людмила Константиновна, кандидат технических наук, доцент кафедры био- нанотехнологии, ФГБОУ ВО «Кемеровский государственный университет» (г. Кемерово), https://orcid.org/0000-0003-4988-8197, alk_kem@mail.ru Дышлюк Любовь Сергеевна, доктор технических наук, доцент кафедры бионанотехно- логии, ФГБОУ ВО «Кемеровский государственный университет» (г. Кемерово), https://orcid.org/0000-0002-7333-8411 Позднякова Анна Владимировна, кандидат технических наук, доцент кафедры биона- нотехнологии, ФГБОУ ВО «Кемеровский государственный университет» (г. Кемерово). Просеков Александр Юрьевич, доктор технических наук, профессор РАН, заведующий кафедрой бионанотехнологии, ФГБОУ ВО «Кемеровский государственный университет» (г. Кемерово), https://orcid.org/0000-0002-5630-3196 Lyudmila K. Asyakina, Candidate of Technical Sciences, Associate Professor of the Department of Bionanotechnology, Kemerovo State University, Kemerovo, https://orcid.org/0000-0003-4988-8197, alk_kem@mail.ru Lyubov S. Dyshlyuk, Doctor of Technical Sciences, Associate Professor of the Department of Bionanotechnology, Kemerovo State University, Kemerovo, https://orcid.org/0000-0002-7333-8411 Anna V. Pozdnyakova, Candidate of Technical Sciences, Associate Professor of the Department of Bionanotechnology, Kemerovo State University, Kemerovo. Alexander Yu. Prosekov, Doctor of Technical Sciences, Professor of the Russian Academy of Sciences, Head of the Department of Bionanotechnology, Kemerovo State University, Kemerovo, https://orcid.org/0000-0002-5630-3196 Получение биологически активных веществ из лекарственных растений на сегодняшний день является актуальной задачей. Пути получения данных веществ разнообразны. В данной статье рассматривается аппаратурное выращивание суспензионных культур клеток и корневых культур in vitro шлемника байкальского и шлемника андрахновидного в биореакторах. Для культивирования предложены: жидкофазный биореактор колонного типа с механическим перемешиванием (скорость перемешивания 50 об./мин), рабочий объем 30 л; жидкофазный биореактор колонного типа с барботажем (пробулькивание стерильных воздуха или газовой смеси, подаваемых снизу), рабочий объем 50 л; жидкофазный биореактор барабанного типа, рабочий объем 100 л; газофазный биореактор туманного типа (диаметр капель питательной среды 20–25 мкм), рабочий объем 30 л; газофазный биореактор дождевального типа (диаметр капель питательной среды 500 мкм), рабочий объем 30 л. Были получены данные о том, что выращивание суспензионных культур шлемника андрахновидного и шлемника байкальского наилучшим способом при использование жидкофазного биореактора колонного типа с барботажем, поскольку в данном случае достигаются максимальные значения индекса роста, удельной скорости роста и экономического коэффициента, а также суммарного содержания основных биохимических соединений. В корневой культуре шлемника байкальского, выращенного в газофазных биореакторах туманного и дождевального типа, зафиксировано максимальное содержание основных биохимических соединений. Для корневой культуры шлемника андрахновидного наибольшее содержание биохимических соединений отмечено при культивировании в газофазном биореакторе туманного типа. The production of biologically active substances from medicinal plants is currently an urgent task. The ways of obtaining these substances are varied. This article discusses the instrumental cultivation of suspension cultures of cells and root cultures in vitro in bioreactors of scutellaria baicalensis and scutellaria andrachnoides. For cultivation the following are offered: liquid-phase column-type bioreactor with mechanical stirring (stirring speed 50 rpm), working volume 30 l; liquid-phase column-type bioreactor with bubbling (bubbling of sterile air or gas mixture supplied from below), working volume 50 l; drum-type liquid-phase bioreactor, working volume 100 l; fog-type gas-phase bioreactor (diameter of nutrient medium droplets 20–25 microns), working volume 30 l; sprinkler-type gas-phase bioreactor (diameter of nutrient medium droplets 500 microns), working volume 30 liters. Data were obtained that the cultivation of suspension cultures of scutellaria andrachnoides and scutellaria baicalensis is the use of a liquid-phase column-type bioreactor with a bubble gum, since in this case the maximum values of the growth index, specific growth rate and economic coefficient, as well as the total content of the main biochemical compounds. In the root culture of scutellaria baicalensis, grown in gas-phase bioreactors of the fog and sprinkler type, the maximum content of the main biochemical compounds was recorded. For the root culture of scutellaria andrachnoides, the highest content of biochemical compounds was noted during cultivation in a fog-type gas-phase bioreactor. Работы выполняются в рамках государственного задания по теме «Скрининг биологически активных веществ растительного происхождения, обладающих геропротекторными свойствами, и разработка технологии получения нутрицевтиков, замедляющих старение» (номер темы FZSR-2020-0006).

Microalgae are a source material in food, pharmacy, and cosmetics industries for producing various products including high-protein nutritional supplements, synthetic pharmaceuticals, and natural colors. A promising algal source for such productions is Chlorella vulgaris which contains a considerable protein content. Similar to other microalgae, its desirability is minimal nutrient requirements since they are unicellular, photosynthetic, and fast-growing microorganisms. Another propitious option to be produced by C. vulgaris is biodiesel, since it is rich in oil too. Besides, algal well thriving in presence of increased amount of carbon dioxide makes them a practicable alternative biofuel resource without some problems of the traditional ones. At the same time, C. vulgaris is also a promising source for nutraceuticals such as amino acids, vitamins, and antioxidants. This review aims to discuss the conditions need to be observed for achieving a favorable growth efficiency of the C. vulgaris, as well as targeted productions such as biomass, antioxidant, and biofuel. Additionally, different approaches to induce any specific production are also considered comprehensively.