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1. The serotonin 5-HT2B receptor from the puffer fish Tetraodon fluviatilis: cDNA cloning, genomic organization and alternatively spliced variants

Author

Irma Nardi, Stefania De Lucchini, Silvia Marracci, DE LUCCHINI, Stefania, Marracci, S., and Nardi, I.

Subjects
DNA, Complementary, Polyadenylation, Molecular Sequence Data, Molecular cloning, Genome, Cellular and Molecular Neuroscience, Complementary DNA, Consensus Sequence, Receptor, Serotonin, 5-HT2B, Animals, Protein Isoforms, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Tetraodon, Molecular Biology, Genomic organization, Genetics, Base Sequence, Sequence Homology, Amino Acid, biology, Tetraodontiformes, Alternative splicing, Intron, biology.organism_classification, Molecular biology, Alternative Splicing, Genes, Receptors, Serotonin, Sequence Alignment
Abstract

We cloned the 5-HT2B serotonin receptor from the puffer fish Tetraodon fluviatilis. Two cDNAs differing in length because of the use of alternative polyadenylation sites were isolated. We partly characterized the genomic organization of the 5-HT2B gene and we found two introns conserved in position between the puffer fish and mammals. In addition, four splice variants which would generate truncated forms of the receptor were detected.

Published
2001
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