15 results on '"María Dolores Vélez"'
Search Results
2. Maritime Pine Genomics in Focus
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Lieven Sterck, Nuria de María, Rafael A. Cañas, Marina de Miguel, Pedro Perdiguero, Annie Raffin, Katharina B. Budde, Miriam López-Hinojosa, Francisco R. Cantón, Andreia S. Rodrigues, Marian Morcillo, Agathe Hurel, María Dolores Vélez, Fernando N. de la Torre, Inês Modesto, Lorenzo Federico Manjarrez, María Belén Pascual, Ana Alves, Isabel Mendoza-Poudereux, Marta Callejas Díaz, Alberto Pizarro, Jorge El-Azaz, Laura Hernández-Escribano, María Ángeles Guevara, Juan Majada, Jerome Salse, Delphine Grivet, Laurent Bouffier, Rosa Raposo, Amanda R. De La Torre, Rafael Zas, José Antonio Cabezas, Concepción Ávila, Jean-Francois Trontin, Leopoldo Sánchez, Ricardo Alía, Isabel Arrillaga, Santiago C. González-Martínez, Célia Miguel, Francisco M. Cánovas, Christophe Plomion, Carmen Díaz-Sala, María Teresa Cervera, Department of Plant Systems Biology, VIB, and Department of Plant Biotechnology and Bioinformatics, Universiteit Gent = Ghent University (UGENT), Vlaams Instituut voor Biotechnologie [Ghent, Belgique] (VIB), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology (INIA), Universidad Politécnica de Madrid (UPM), Universidad de Málaga [Málaga] = University of Málaga [Málaga], Biodiversité, Gènes & Communautés (BioGeCo), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Instituto de Biologia Experimental e Tecnológica (IBET), Centro de Investigacion en Sanidad Animal (INIA-CISA), Unité expérimentale Forêt Pierroton (UEFP), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Georg-August-University = Georg-August-Universität Göttingen, Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Swammerdam Institute for Life Sciences (SILS), University of Amsterdam [Amsterdam] (UvA), Universitat de València (UV), Faculdade de Ciências [Lisboa], Universidade de Lisboa = University of Lisbon (ULISBOA), Universidad de Alcalá - University of Alcalá (UAH), Forest and Wood Technology Research Centre (CETEMAS), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Northern Arizona University [Flagstaff], Mision Biologica de Galicia (MBG), Institut Technologique Forêt Cellulose Bois-construction Ameublement (FCBA), Biologie intégrée pour la valorisation de la diversité des Arbres et de la Forêt (BioForA), Office national des forêts (ONF)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Amanda R. De La Torre
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Conifer genomics ,[SDV.GEN]Life Sciences [q-bio]/Genetics ,Development and adaptation ,[SDE.MCG]Environmental Sciences/Global Changes ,fungi ,[SDV.BID]Life Sciences [q-bio]/Biodiversity ,Genome sequencing ,[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] ,[SDV.SA.SF]Life Sciences [q-bio]/Agricultural sciences/Silviculture, forestry ,[STAT.ML]Statistics [stat]/Machine Learning [stat.ML] ,Molecular breeding ,Gene expression ,Mediterranean pine ,[MATH]Mathematics [math] ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology - Abstract
International audience; The advent of next-generation genome sequencing technologies has allowed approaching the sequencing and analysis of large and complex conifer genomes. Maritime pine (Pinus pinaster Ait.) is an economically and ecologically important conifer species widely distributed in South-West Europe, which shows a significant genetic and adaptive variability. This chapter takes on the task of reviewing the insights into the maritime pine genome sequencing breakthrough and its impact on downstream analysis. Maritime pine genome sequencing and assembly approaches are described along with the impact of related tools. A section of the state-of-the-art research on comparative, functional, structural, and translational genomics aimed at dissecting the genetic basis and the specific regulation of biological processes underlying the expression of traits of interest in maritime pine and other conifers is also described. Perspectives about the impact of these tools as well as additional research approaches are discussed.
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- 2022
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3. Correlating the above- and belowground genotype of Pinus pinaster trees and rhizosphere bacterial communities under drought conditions
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Ana V. Lasa, M. Ángeles Guevara, Pablo J. Villadas, María Dolores Vélez, Antonio J. Fernández-González, Nuria de María, Miriam López-Hinojosa, Luis Díaz, María Teresa Cervera, Manuel Fernández-López, Ministerio de Economía, Industria y Competitividad (España), Ministerio de Ciencia e Innovación (España), Ministerio de Ciencia, Innovación y Universidades (España), Lasa, Ana V [0000-0003-3783-7157], Guevara, M Ángeles [0000-0001-7399-3136], Villadas, Pablo J [0000-0001-8662-8883], Vélez, María Dolores [0000-0001-8871-5737], Fernández-González, Antonio J[0000-0002-1938-5436], de María, Nuria [0000-0003-4506-3980], López-Hinojosa, Miriam [0000-0002-7495-1517], Díaz, Luis [0000-0002-7495-1517], Lasa, Ana V, Guevara, M Ángeles, Villadas, Pablo J, Vélez, María Dolores, Fernández-González, Antonio J, de María, Nuria, López-Hinojosa, Miriam, and Díaz, Luis
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Environmental Engineering ,Tree genotype ,Bacteria ,Genotype ,Drought ,Microbial co-occurrence networks ,Pinus ,Plant Roots ,Pollution ,Droughts ,Trees ,Mediterranean basi ,Rhizosphere ,Metabarcoding ,RNA ,Environmental Chemistry ,Waste Management and Disposal ,Soil Microbiology - Abstract
16 Pág., Increasing temperatures along with severe droughts are factors that may jeopardize the survival of the forests in the Mediterranean basin. In this region, Pinus pinaster is a common conifer species, that has been used as a model species in evolutionary studies due to its adaptive response to changing environments. Although its drought tolerance mechanisms are already known, knowledge about the dynamics of its root microbiota is still scarce. We aimed to decipher the structural (bacterial abundance), compositional, functional and associative changes of the P. pinaster rhizosphere bacterial communities in spring and summer, at DNA and RNA level (environmental DNA, live and dead cells, and those synthesizing proteins). A fundamental aspect of root microbiome-based approaches is to guarantee the correct origin of the samples. Thus, we assessed the genotype of host needles and roots from which rhizosphere samples were obtained. For more than 50% of the selected trees, genotype discrepancies were found and in three cases the plant species could not be determined. Rhizosphere bacterial communities were homogeneous with respect to diversity and structural levels regardless of the host genotype in both seasons. Nonetheless, significant changes were seen in the taxonomic profiles depending on the season. Seasonal changes were also evident in the bacterial co-occurrence patterns, both in DNA and RNA libraries. While spring communities switched to more complex networks, summer populations resulted in more compartmentalized networks, suggesting that these communities were facing a disturbance. These results may mirror the future status of bacterial communities in a context of climate change. A keystone hub was ascribed to the genus Phenylobacterium in the functional network calculated for summer. Overall, it is important to validate the origin and identity of plant samples in any plant-microbiota study so that more reliable ecological analyses are performed., This work was supported by the Spanish Ministry of Economy, Industry and Competitiveness [AGL2015-66048-C2-1-R; RTI2018-098015-B-I00], and by the Spanish Ministry of Science and Innovation project SUMHAL [LIFEWATCH-2019-09-CSIC-13, POPE 2014-2020], both co-financed by the (ERDF)European Regional Development Fund
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- 2022
4. Treatment of cutaneous leiomyomas with botulinum toxin. Case report and review of literature
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Ana Rodríguez-Villa Lario, Isabel Polo-Rodríguez, Susana Medina, María Dolores Vélez-Velázquez, Marta González-Cañete, and David Vega-Díez
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medicine.medical_specialty ,Leiomyoma ,Cutaneous Leiomyoma ,business.industry ,Medicine ,Dermatology ,General Medicine ,business ,medicine.disease ,Botulinum toxin ,Pain therapy ,medicine.drug - Published
- 2021
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5. Rootstock effects on scion gene expression in maritime pine
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Alberto Pizarro, L F Manjarrez, José Antonio Cabezas, Carmen Díaz-Sala, Carmen Collada, M T Cervera Goy, María Dolores Vélez, M. A. Guevara, N. De Maria, Miriam López-Hinojosa, Luis-Manuel Díaz, J A Mancha, Ministerio de Economía, Industria y Competitividad (España), Universidad de Alcalá, Lopez-Hinojosa, M. [0000-0002-7495-1517], de Maria, N.[0000-0003-0743-9465], Velez, M.D. [0000-0001-8871-5737], Pizarro, A. [0000-0002-0346-3743], Collada, C. [0000-0003-0236-1312], Lopez-Hinojosa, M., de Maria, N., Velez, M.D., Pizarro, A., and Collada, C.
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0106 biological sciences ,0301 basic medicine ,Molecular biology ,Science ,Growth ,Biology ,Genes, Plant ,Stress ,Plant Roots ,01 natural sciences ,Article ,Transcriptome ,03 medical and health sciences ,Differential expression ,Gene Expression Regulation, Plant ,Gene expression ,Botany ,Genetics ,Transcriptional regulation ,Transcription factors ,Drought tolerance, Pinaster AIT ,Acid ,Gas-Exchange ,Secondary metabolism ,Patterns ,Gene ,Multidisciplinary ,Abiotic stress ,Protein ,fungi ,Pinus ,biology.organism_classification ,Adaptation, Physiological ,Droughts ,030104 developmental biology ,Pinus pinaster ,Medicine ,Plant sciences ,Rootstock ,010606 plant biology & botany - Abstract
Pines are the dominant conifers in Mediterranean forests. As long-lived sessile organisms that seasonally have to cope with drought periods, they have developed a variety of adaptive responses. However, during last decades, highly intense and long-lasting drought events could have contributed to decay and mortality of the most susceptible trees. Among conifer species, Pinus pinaster Ait. shows remarkable ability to adapt to different environments. Previous molecular analysis of a full-sib family designed to study drought response led us to find active transcriptional activity of stress-responding genes even without water deprivation in tolerant genotypes. To improve our knowledge about communication between above- and below-ground organs of maritime pine, we have analyzed four graft-type constructions using two siblings as rootstocks and their progenitors, Gal 1056 and Oria 6, as scions. Transcriptomic profiles of needles from both scions were modified by the rootstock they were grafted on. However, the most significant differential gene expression was observed in drought-sensitive Gal 1056, while in drought-tolerant Oria 6, differential gene expression was very much lower. Furthermore, both scions grafted onto drought-tolerant rootstocks showed activation of genes involved in tolerance to abiotic stress, and is most remarkable in Oria 6 grafts where higher accumulation of transcripts involved in phytohormone action, transcriptional regulation, photosynthesis and signaling has been found. Additionally, processes, such as those related to secondary metabolism, were mainly associated with the scion genotype. This study provides pioneering information about rootstock effects on scion gene expression in conifers., This work was supported by the Spanish Ministry of Economy, Industry and Competitiveness (AGL2015-66048-C2-1-R; RTI2018-098015-B-I00), and by University of Alcalá (UAH-AE 2017-2).
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- 2021
6. Aquagenic keratoderma with dorsal involvement treated with botulinum toxin. Case report and review of literature
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Alba Gómez-Zubiaur, David Vega-Díez, Susana Medina-Montalvo, Lidia Trasobares-Marugán, Marta González-Cañete, Isabel Polo-Rodríguez, Ana Rodríguez-Villa Lario, and María Dolores Vélez-Velázquez
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Dorsum ,medicine.medical_specialty ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Dermatology ,General Medicine ,medicine.disease ,Botulinum toxin ,medicine ,business ,Keratoderma ,medicine.drug - Published
- 2020
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7. Comprehensive analysis of the isomiRome in the vegetative organs of the conifer Pinus pinaster under contrasting water availability
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A.C. Alves, Bruno Costa, Carmen Díaz-Sala, Inês Chaves, María Dolores Vélez, Célia Miguel, Pedro Perdiguero, Nuria de María, Andreia S. Rodrigues, María Teresa Cervera, European Commission, Fundação para a Ciência e a Tecnologia (Portugal), Ministerio de Economía, Industria y Competitividad (España), Perdiguero, Pedro, Rodrigues, Andreia Santos, Chaves, Inês, Costa, Bruno, Alves, Ana, de María, Nuria0000-0003-4506-3980, Vélez, María Dolores, Díaz-Sala, Carmen, Cervera, María Teresa, and Miguel, Célia Maria
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0106 biological sciences ,0301 basic medicine ,Small RNA ,Physiology ,Water stress ,Plant Science ,IsomiR ,Genes, Plant ,01 natural sciences ,Genome ,Environmental stress ,Plant Roots ,03 medical and health sciences ,microRNA ,Organ specificity ,biology ,Dehydration ,Plant Stems ,fungi ,15. Life on land ,biology.organism_classification ,Pinus ,Pine ,Plant Leaves ,MicroRNAs ,030104 developmental biology ,Evolutionary biology ,RNA, Plant ,Pinus pinaster ,Target genes ,Adaptation ,MiRNA ,MiRNA biogenesis ,Transcriptome ,010606 plant biology & botany - Abstract
23 Pág. Centro de Investigación en Sanidad Animal (CISA), An increasing number of microRNAs (miRNAs) and miRNA-related sequences produced during miRNA biogenesis, comprising the isomiRome, have been recently highlighted in different species as critical mediators of environmental stress responses. Conifers have some of the largest known genomes but an extensive characterization of the isomiRome from any conifer species has been lacking. We provide here a comprehensive overview of the Pinus pinaster isomiRome expressed in roots, stem and needles under well-watered and drought conditions. From the 13,441 unique small RNA sequences identified, 2,980 were annotated as canonical miRNAs or miRNA* and the remaining were classified as isomiRNA or miRNA-like sequences. A survey of their expression patterns highlighted roots as the most responsive organ under drought, where specific sequences of which a 24-nt novel miRNA stood out, were strongly down-regulated. Given the putative roles of the miRNA-targeted transcripts validated specifically in root tissues, some of the miRNAs, conserved and novel, are shortlisted as potential regulators of drought response. These results provide a valuable resource for comparative studies between gymnosperms and angiosperms. Furthermore, it evidences high transferability of the isomiRome between pine species being a useful basis for further molecular regulation and physiological studies, and especially those focused on adaptation to drought conditions., FP7 People: Marie-Curie Actions, Grant/Award Number: PIEF-GA-2013-627761; Fundação para a Ciência e a Tecnologia, Grant/Award Numbers: IF/01168/2013, SFRH/BD/79779/2011; SFRH/BD/128827/2017; UID/Multi/04551/2013; UID/Multi/04046/2019; DL57/2016/CP1351/CT0003; Horizon 2020 Framework Programme, Grant/Award Number: 676559; Ministerio de Economía, Industria y Competitividad, Gobierno de España, Grant/Award Numbers: AGL2015-66048-C2-1-R, RTI2018-098015-B-I00; Seventh Framework Programme, Grant/Award Number: 289841
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- 2020
8. Síndrome de Wells’: presentación de 2 casos y revisión de la literatura
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Alba Gómez-Zubiaur, Susana Medina-Montalvo, Sonia Beà-Ardébol, María Dolores Vélez-Velázquez, A.B. Piteiro-Bermejo, and Lidia Trasobares-Marugán
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030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,business.industry ,030225 pediatrics ,Medicine ,Dermatology ,business - Published
- 2018
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9. Eccrine Porocarcinoma: Patient Characteristics, Clinical and Histopathologic Features, and Treatment in 7 Cases
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Susana Medina-Montalvo, Isabel Polo-Rodríguez, Alba Gómez-Zubiaur, and María Dolores Vélez-Velázquez
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Histology ,Clinical Decision-Making ,Sentinel lymph node ,Patient characteristics ,Dermatology ,Eccrine porocarcinoma ,Pathology and Forensic Medicine ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Biopsy ,medicine ,Carcinoma ,Humans ,In patient ,Aged ,Aged, 80 and over ,medicine.diagnostic_test ,Sentinel Lymph Node Biopsy ,business.industry ,Disease Management ,Eccrine Porocarcinoma ,Middle Aged ,Prognosis ,medicine.disease ,Sweat Gland Neoplasms ,Lymphatic Metastasis ,030220 oncology & carcinogenesis ,Lymph Node Excision ,Female ,Radiotherapy, Adjuvant ,Lymph ,business - Abstract
Eccrine porocarcinoma is a rare, malignant cutaneous adnexal tumor that arises from the ducts of sweat glands. Found mainly in patients of advanced age, this tumor has diverse clinical presentations. Histology confirms the diagnosis, detects features relevant to prognosis, and guides treatment. Growth is slow, but the prognosis is poor if the tumor metastasizes to lymph nodes or visceral organs. We report 7 cases of eccrine porocarcinoma, describing patient characteristics, the clinical and histopathologic features of the tumors, and treatments used. Our observations were similar to those of other published case series. Given the lack of therapeutic algorithms or protocols for this carcinoma, we propose a decision-making schema based on our review of the literature and our experience with this case series. The algorithm centers on sentinel lymph node biopsy and histologic features.
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- 2017
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10. Porocarcinoma ecrino: aspectos demográficos, clínicos, histopatológicos y terapéuticos en una serie de 7 pacientes
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Susana Medina-Montalvo, Isabel Polo-Rodríguez, María Dolores Vélez-Velázquez, and Alba Gómez-Zubiaur
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030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,General Medicine - Abstract
Resumen El porocarcinoma ecrino es un tumor anexial cutaneo maligno poco frecuente, que deriva de la porcion ductal de las glandulas sudoriparas. Predomina en pacientes de edad avanzada, pudiendo adoptar diversas presentaciones clinicas. El estudio histologico confirma el diagnostico y establece factores pronosticos relevantes a la hora de decidir el tratamiento. El curso evolutivo es lento, pero su pronostico es sombrio cuando aparecen metastasis linfaticas o viscerales. Presentamos 7 pacientes diagnosticados de porocarcinoma ecrino, y describimos los aspectos epidemiologicos, clinicos, histopatologicos y los datos relacionados con el tratamiento de cada uno de ellos. Comparamos los datos obtenidos con las mayores series de casos publicadas, obteniendo similares resultados. Ante la ausencia de algoritmos terapeuticos protocolizados se propone un esquema de tratamiento basado en la literatura revisada y la experiencia personal con nuestra serie, que tiene como ejes centrales los factores pronosticos histologicos y la biopsia selectiva de ganglio centinela.
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- 2017
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11. Fibro-Osseous Pseudotumor of the Digits Mimicking Pyogenic Granuloma
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Alba Gómez-Zubiaur, Susana Medina-Montalvo, Alicia Cabrera-Hernández, María Dolores Vélez-Velázquez, A.B. Piteiro-Bermejo, Lidia Trasobares-Marugán, Laura Pericet-Fernández, and Sonia Beà-Ardébol
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0301 basic medicine ,medicine.medical_specialty ,Adolescent ,Soft Tissue Neoplasms ,Dermatology ,Fibroma ,Granuloma, Plasma Cell ,Diagnosis, Differential ,Fingers ,03 medical and health sciences ,0302 clinical medicine ,Rare Diseases ,medicine ,Humans ,Granuloma, Pyogenic ,Pyogenic granuloma ,business.industry ,Biopsy, Needle ,Soft tissue ,Osteoma ,medicine.disease ,Immunohistochemistry ,stomatognathic diseases ,030104 developmental biology ,Treatment Outcome ,030220 oncology & carcinogenesis ,Pediatrics, Perinatology and Child Health ,Female ,Radiology ,Fibro-osseous pseudotumor ,business ,Surgical interventions - Abstract
Fibro-osseous pseudotumor of the digits is a benign neoplasm that originates in the soft tissue adjacent to the short bones of the hands and feet. We present a case in a 13-year-old girl that was initially misdiagnosed as pyogenic granuloma. Familiarity with this entity and imaging and histologic studies are necessary to avoid incorrect diagnoses and aggressive surgical interventions.
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- 2017
12. Analysis of DNA Cytosine Methylation Patterns Using Methylation-Sensitive Amplification Polymorphism (MSAP)
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María Ángeles, Guevara, Nuria, de María, Enrique, Sáez-Laguna, María Dolores, Vélez, María Teresa, Cervera, and José Antonio, Cabezas
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Epigenomics ,Cytosine ,CpG Islands ,Amplified Fragment Length Polymorphism Analysis ,DNA Methylation ,Epigenesis, Genetic - Abstract
Different molecular techniques have been developed to study either the global level of methylated cytosines or methylation at specific gene sequences. One of them is the methylation-sensitive amplified polymorphism technique (MSAP) which is a modification of amplified fragment length polymorphism (AFLP). It has been used to study methylation of anonymous CCGG sequences in different fungi, plants, and animal species. The main variation of this technique resides on the use of isoschizomers with different methylation sensitivity (such as HpaII and MspI) as a frequent-cutter restriction enzyme. For each sample, MSAP analysis is performed using both EcoRI/HpaII- and EcoRI/MspI-digested samples. A comparative analysis between EcoRI/HpaII and EcoRI/MspI fragment patterns allows the identification of two types of polymorphisms: (1) methylation-insensitive polymorphisms that show common EcoRI/HpaII and EcoRI/MspI patterns but are detected as polymorphic amplified fragments among samples and (2) methylation-sensitive polymorphisms which are associated with the amplified fragments that differ in their presence or absence or in their intensity between EcoRI/HpaII and EcoRI/MspI patterns. This chapter describes a detailed protocol of this technique and discusses the modifications that can be applied to adjust the technology to different species of interest.
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- 2016
13. Analysis of DNA Cytosine Methylation Patterns Using Methylation-Sensitive Amplification Polymorphism (MSAP)
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José Antonio Cabezas, María Angeles Guevara, María Teresa Cervera, Nuria de María, Enrique Sáez-Laguna, and María Dolores Vélez
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0106 biological sciences ,0301 basic medicine ,Genetics ,HpaII ,viruses ,EcoRI ,Methylation ,Biology ,01 natural sciences ,03 medical and health sciences ,Restriction enzyme ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Isoschizomer ,DNA methylation ,biology.protein ,Amplified fragment length polymorphism ,DNA ,010606 plant biology & botany - Abstract
Different molecular techniques have been developed to study either the global level of methylated cytosines or methylation at specific gene sequences. One of them is the methylation-sensitive amplified polymorphism technique (MSAP) which is a modification of amplified fragment length polymorphism (AFLP). It has been used to study methylation of anonymous CCGG sequences in different fungi, plants, and animal species. The main variation of this technique resides on the use of isoschizomers with different methylation sensitivity (such as HpaII and MspI) as a frequent-cutter restriction enzyme. For each sample, MSAP analysis is performed using both EcoRI/HpaII- and EcoRI/MspI-digested samples. A comparative analysis between EcoRI/HpaII and EcoRI/MspI fragment patterns allows the identification of two types of polymorphisms: (1) methylation-insensitive polymorphisms that show common EcoRI/HpaII and EcoRI/MspI patterns but are detected as polymorphic amplified fragments among samples and (2) methylation-sensitive polymorphisms which are associated with the amplified fragments that differ in their presence or absence or in their intensity between EcoRI/HpaII and EcoRI/MspI patterns. This chapter describes a detailed protocol of this technique and discusses the modifications that can be applied to adjust the technology to different species of interest.
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- 2016
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14. Central centrifugal cicatricial alopecia following a patchy pattern: A new form of clinical presentation and a challenging diagnosis for the dermatologist
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Lidia Trasobares-Marugán, David Saceda-Corralo, Ana Rodríguez-Villa Lario, Alba Gómez-Zubiaur, and María Dolores Vélez-Velázquez
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Central centrifugal cicatricial alopecia ,medicine.medical_specialty ,cicatricial alopecia ,integumentary system ,business.industry ,Case Report ,Dermatology ,medicine.disease ,Trichoscopy ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Hair loss ,030220 oncology & carcinogenesis ,Scalp ,patchy pattern ,medicine ,Centrifugal ,Differential diagnosis ,Presentation (obstetrics) ,business - Abstract
Central centrifugal cicatricial alopecia (CCCA) is included among the primary lymphocytic cicatricial alopecias. The current nomenclature of CCCA suggested by the North American Hair Research Society refers to the traditional clinical presentation pattern of this type of alopecia, which begins in the central area of the scalp and has a progressive and symmetric centrifuge evolution. However, some exceptions should be highlighted, and a new clinical variety of CCCA presenting with patches of hair loss affecting the lateral and posterior scalp has been recently described. Here, we report a new case of CCCA presenting with a patchy pattern. In addition to the fact that this new patchy presentation of CCCA is not contemplated in the current terminology, it has a difficult differential diagnosis with other alopecias that have patches as their presentation. In these cases, both the trichoscopy and the histopathology are indispensable for diagnosis that will allow a targeted treatment and avoid an unfortunate prognosis.
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- 2019
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15. Influence of the farming system and vine variety on yeast communities associated with grape berries
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María Dolores Vélez, Javier Tello, Gustavo Cordero-Bueso, Teresa Arroyo, Eva Valero, Ana Serrano, and Irene Aporta
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Vine ,Genotype ,Biodiversity ,Wine ,Saccharomyces cerevisiae ,Biology ,Polymerase Chain Reaction ,Microbiology ,Vineyard ,Pichia ,Yeasts ,Botany ,Vitis ,DNA, Fungal ,Candida ,Phytosanitary certification ,Organic Agriculture ,food and beverages ,Agriculture ,General Medicine ,Random Amplified Polymorphic DNA Technique ,Colonisation ,Horticulture ,Spain ,Fermentation ,Organic farming ,Species richness ,Polymorphism, Restriction Fragment Length ,Microsatellite Repeats ,Food Science - Abstract
Wine production in most countries is based on the use of commercial strains leading to the colonisation of the wineries by these strains with the consequent reduction of autochthonous biodiversity. This implies that wine styles could therefore become standardised. The vineyard could be an important source of native yeasts of oenological interest. For this reason the objective of this study was to compare two agronomic conditions with the aim of preserving yeast biodiversity in the vineyard. A three year sampling plan was designed to evaluate the influence of different agronomic parameters on the biodiversity of fermentative grape yeasts. Thus two vineyards, one organic and one conventional, with three different grape varieties (Shiraz, Grenache and Barbera) were chosen. In total, 27 samples were collected from both vineyards. Of these, 1080 colonies were isolated and a total of 9 species were identified. The strains identified as Saccharomyces cerevisiae were genotyped by microsatellite analysis obtaining nine different electrophoretic patterns. Classical ecology indexes were used to obtain the richness (S), the biodiversity (H') and the dominance (D) of the species studied. The results indicated a clear influence on grape associated yeast diversity of the phytosanitary treatment used in the vineyard. This is the first time that classical ecology indexes have been used to study the ecology of the spontaneous fermentation of grape musts and the species Candida sorbosa and Pichia toletana have been described in vineyards of the Madrid winegrowing region.
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- 2011
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