José Manuel Santos-Lozano, José Lapetra, José M. Castellano, Mirella Rada, Ángeles Guinda, L. Macías, Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, [Castellano, J. M.] CSIC, Food & Hlth Dept, Inst Grasa, Campus Univ Pablo de Olavide, Seville, Spain, [Guinda, A.] CSIC, Food & Hlth Dept, Inst Grasa, Campus Univ Pablo de Olavide, Seville, Spain, [Macias, L.] CSIC, Food & Hlth Dept, Inst Grasa, Campus Univ Pablo de Olavide, Seville, Spain, [Rada, M.] CSIC, Food & Hlth Dept, Inst Grasa, Campus Univ Pablo de Olavide, Seville, Spain, [Santos-Lozano, J. M.] Inst Salud Carlos III, CIBER Physiopathol Obes & Nutr Ciberobn, Madrid, Spain, [Lapetra, J.] Inst Salud Carlos III, CIBER Physiopathol Obes & Nutr Ciberobn, Madrid, Spain, [Santos-Lozano, J. M.] Primary Care Ctr San Pablo, Primary Care Hlth Dist Seville, Dept Family Med, Seville, Spain, [Lapetra, J.] Primary Care Hlth Dist Seville, Res Unit, Dept Family Med, Seville, Spain, and Spanish Ministry of Economy and Competitiveness (Carlos III Institute of Health)
11 Páginas.-- 6 Figuras.-- 1 Tabla, This work investigates the role of oleanolic acid (OA), isolated from the olive (Olea europaea L.) leaf, as a radical scavenger and inhibitor of the hydrolyzing enzymes of dietary carbohydrates. New evidence is provided showing that OA may capture 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) and peroxyl radicals, and also exert a strong and non-competitive inhibition of a-glucosidase (IC 10.11 ± 0.30 μM). The kinetic and spectrometric analyses performed indicate that OA interacts with this enzyme inside a hydrophobic pocket, through an endothermic and non spontaneous process of a hydrophobic nature. These are two possible mechanisms by which OA may facilitate a better control of post-prandial hyperglycaemia and oxidative stress, so contributing to preserving insulin signalling. Obesity, insulin resistance and Type 2 Diabetes Mellitus are considered the first pandemics of the 21st century. In this sense, OA might be used in future preventive and therapeutic strategies, as an ingredient in new drugs and functional foods., The Spanish Ministry of Economy and Competitiveness (Carlos III Institute of Health) financed the research project PI10/01415. The authors wish to thank the Andalusian Public System of Health (Spain) and the ACESUR Group for making the PREDIABOLE Study possible.