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Supplemental material, sj-pptx-4-ijs-10.1177_10668969221133351 for Adult Pancreatoblastoma: Report of 3 new Cases With Genetic Diversity and Autopsy Findings by Isidro Machado, José Antonio López-Guerrero, Antonio Fernandez, Raquel López, Zaida García Casado, Antonio Ferrandez, Antonio Llombart-Bosch and Gregory W. Charville in International Journal of Surgical Pathology

Supplemental material, sj-pptx-2-ijs-10.1177_10668969221133351 for Adult Pancreatoblastoma: Report of 3 new Cases With Genetic Diversity and Autopsy Findings by Isidro Machado, José Antonio López-Guerrero, Antonio Fernandez, Raquel López, Zaida García Casado, Antonio Ferrandez, Antonio Llombart-Bosch and Gregory W. Charville in International Journal of Surgical Pathology

Supplemental material, sj-pptx-3-ijs-10.1177_10668969221133351 for Adult Pancreatoblastoma: Report of 3 new Cases With Genetic Diversity and Autopsy Findings by Isidro Machado, José Antonio López-Guerrero, Antonio Fernandez, Raquel López, Zaida García Casado, Antonio Ferrandez, Antonio Llombart-Bosch and Gregory W. Charville in International Journal of Surgical Pathology

Background. Chondrosarcomas (Chs) are malignant cartilage-forming tumors that represent the third most common malignant solid tumor of bone in adults. Angiogenesis is a major factor for tumor growth and metastasis. Our aim was to make a histological, immunohistochemical, ultrastructural and molecular characterization of the neovascularization established between xenotransplanted Chs and the host during the initial phases of growth in nude transfer, in order to find potential markers for distinguishing between high grades II and III Chs. Methods. two xenotransplanted high grade human Chs were evaluated. Tumor pieces were implanted subcutaneously on the backs of 14 athymic Balb-c nude mice. The animals were sacrificed 24, 48, and 96 hours; and 7, 14, 21 and 28 days after implantation. Two grade I Chs were also transferred in nude but did not grow. Results. Morphological differences were apparent between these two Chs during the early stages of neoplastic growth. Immunohistochemistry demonstrated overexpression of pro-angiogenic factors 24h-48h after tumor implantation. Additionally, neoplastic cells co-expressed chemokines (CXCL9, CXCL10 and GRO) and their receptors. Molecular studies showed two expression profiles, revealing an early and a late phase in the angiogenic process. Conclusion. High grade Chs demonstrated two different stages of induced angiogenesis, with an intimate association between structural and molecular events that might explain the different aggressive biological behavior of grade II and III Chs. The present model may be useful for testing the effect of anti-angiogenic drugs.

Ewing-like sarcomas (ELS) are a heterogenous group of tumors that frequently affect pediatric and young adult patients. Accurate classification and distinction from the Ewing sarcoma family of tumor (ESFT) is decisive in patient management. ELS share a significant morphologic, immunohistochemical and clinical overlap with ESFT, thus the differential diagnosis is challenging, especially with atypical ESFT and tumors with unusual immunoprofiles or uncommon clinicoradiological findings. A subset of ELS harboring the CIC-DUX4 or BCORCCNB3 fusions has been described recently. The spectrum of ELS is now expanding, and additional gene fusion partners besides DUX4 or CCNB3 have been detected, and the terms CIC or BCOR-rearranged sarcomas have recently been proposed. We review the clinical, histological, phenotypic and molecular findings of ESFT and these new emerging ELS.