1. [Unusual forms of persistence of Mycoplasma hominis in organism of infected humans]
- Author
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G A, Levina, O I, Barkhatova, L G, Gorina, N A, Gamova, S A, Goncharova, Miller G G, T M, Raskova, I N, Rastegaeva, N A, Seliverstova, and I V, Rakovskaia
- Subjects
DNA, Bacterial ,Antigen-Antibody Complex ,Real-Time Polymerase Chain Reaction ,Adaptation, Physiological ,Immunity, Humoral ,Polyethylene Glycols ,Mycoplasma hominis ,Mice ,Microscopy, Electron ,RNA, Ribosomal, 16S ,Animals ,Chemical Precipitation ,Humans ,Mycoplasma Infections - Abstract
Study previously unknown forms of persistence of Mycoplasma hominis in host organism.Culture method was used for detection of mycoplasmas. Identification was carried out by serological, electron microscopy methods, classic PCR and real time PCR; circulating immune complexes (CIC) were isolated by PEG precipitation.Classic micoplasma cultures could not be isolated from blood even once. At the same time "mini-colony" cultures composed of mini-cells that were hardly passaged but sometimes formed continuous layer of the same colonies were isolated from blood serum samples with high frequency. During reseeding for more than 1 year they never acquired classic form. Not only antigens of M. hominis but its DNA were shown to be present in CIC. Viable cells forming "mini-colonies" identical to those isolated from blood sera were isolated from circulating immune complexes. A system of evidence on identity of isolated M. hominis cultures is presented. Cultures had infectivity and an ability to persist in organs of experimentally infected mice.The isolated forms are apparently the result of adaptation of mycoplasmas to humoral immunity factors.
- Published
- 2012