1. Immunohistochemical localization of integrin alpha V beta 3 and osteopontin suggests that they do not interact during embryo implantation in ruminants
- Author
-
MacLaren Leslie A, Lim Hai, Kimmins Sarah, Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, Department of Plant and Animal Sciences, Nova Scotia Agricultural College, This work was financed by an NSERC postgraduate scholarship to S. K. and grants to L.M. from the Dairy Farmers of Canada, the Nova Scotia Technology Development Program and NSERC., Maylin, Françoise, and Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Male ,lcsh:QH471-489 ,[SDV.IMM] Life Sciences [q-bio]/Immunology ,Sialoglycoproteins ,MESH: Sheep ,Estrous Cycle ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,MESH: Research Support, Non-U.S. Gov't ,lcsh:Gynecology and obstetrics ,Epithelium ,MESH: Embryo Implantation ,Endometrium ,MESH: Pregnancy ,Pregnancy ,lcsh:Reproduction ,MESH: Protein Binding ,Animals ,MESH: Animals ,Embryo Implantation ,MESH: Sialoglycoproteins ,[SDV.BC] Life Sciences [q-bio]/Cellular Biology ,lcsh:RG1-991 ,[SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology ,Sheep ,Research ,MESH: Comparative Study ,MESH: Immunohistochemistry ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,Integrin alphaVbeta3 ,Immunohistochemistry ,MESH: Male ,MESH: Cattle ,MESH: Epithelium ,MESH: Endometrium ,MESH: Integrin alphaVbeta3 ,MESH: Estrous Cycle ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,Cattle ,Female ,Osteopontin ,MESH: Female ,Protein Binding - Abstract
Background It has been suggested that trophoblast attachment requires co-expression of integrin alpha V beta 3 and its ligand osteopontin at the fetal-maternal interface. Until now the expression patterns of integrin alpha V beta 3 and osteopontin in the pregnant bovine uterus were unknown. The objectives of this study were to localize integrin alpha V beta 3 and osteopontin in bovine and sheep endometrium during the periimplantation period and to compare the distribution patterns using antibodies that had not yet been tested in sheep. Methods Cell compartments within endometrial tissue sections were scored for immunohistochemical staining intensity and data were analyzed to determine the effects of day of pregnancy or cycle. Results In pregnant bovine endometrium, integrin alpha V beta 3 was detected in luminal epithelium, stroma, myometrium and smooth muscle. A strong band of immunoreactivity was observed in the subepithelial stroma of intercaruncular regions, but there was reduced reactivity in the caruncles and glands. Bovine trophoblast did not express integrin alpha V beta 3 at any stage of pregnancy. In ovine endometrium a different pattern of staining for integrin alpha V beta 3 was observed. Reactivity was not present in the luminal epithelium or trophoblast. There was strong staining of the deep glands and no reactivity in the superficial glands. Osteopontin distribution was similar for sheep and cattle. For both species, apical staining was present on the luminal epithelium and glands and on embryonic tissues. Conclusion In ruminants, integrin alpha V beta 3 and osteopontin do not co-localize at the fetal-maternal interface indicating that these proteins could not interact to facilitate embryo attachment as has been proposed in other species.
- Published
- 2003