1. Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer
- Author
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Christian J. Kinane, Timothy R. Dafforn, Thomas Arnold, Luke A. Clifton, Kerrie A. Morrison, Stephen Hall, Timothy J. Knowles, Karen J. Edler, and Cecilia Tognoloni
- Subjects
SMAnh, poly(styrene-co-maleic anhydride) ,02 engineering and technology ,RAFT, reversible addition-fragmentation chain transfer ,01 natural sciences ,ATR-FTIR, attenuated total reflection Fourier transform infrared ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,SLD, scattering length density ,Copolymer ,SMA, poly(styrene-co-maleic acid) ,RAFT-SMA, RAFT-synthesised SMA ,Lipid bilayer ,chemistry.chemical_classification ,Host cell membrane ,Neutron reflectometry ,Chemistry ,SMALP, SMA lipid particle ,MCMC, Markov chain Monte Carlo ,MWCO, molecular weight cut-off ,Polymer ,021001 nanoscience & nanotechnology ,SMI, poly(styrene-co-maleimide) ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,NaOAc, sodium acetate ,lipids (amino acids, peptides, and proteins) ,dDMPC, 1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine ,SMILP, SMI lipid particle ,Lipid exchange ,0210 nano-technology ,Supported lipid bilayer ,SiMW, silicon-matched water ,Phospholipid ,010402 general chemistry ,Polymer-stabilized phospholipid nanodisc ,Article ,Biomaterials ,Amphiphile ,DMPC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine ,Nanodisc ,ComputingMethodologies_COMPUTERGRAPHICS ,MSP, membrane scaffold protein ,styrene maleic acid (SMA) ,0104 chemical sciences ,MP, membrane protein ,Chemical engineering ,Polymerization ,styrene-maleic acid lipid particle (SMALP) ,NR, neutron reflectometry ,SEC, size exclusion chromatography ,Adsorption - Abstract
Graphical abstract, Over recent years, there has been a rapid development of membrane-mimetic systems to encapsulate and stabilize planar segments of phospholipid bilayers in solution. One such system has been the use of amphipathic copolymers to solubilize lipid bilayers into nanodiscs. The attractiveness of this system, in part, stems from the capability of these polymers to solubilize membrane proteins directly from the host cell membrane. The assumption has been that the native lipid annulus remains intact, with nanodiscs providing a snapshot of the lipid environment. Recent studies have provided evidence that phospholipids can exchange from the nanodiscs with either lipids at interfaces, or with other nanodiscs in bulk solution. Here we investigate kinetics of lipid exchange between three recently studied polymer-stabilized nanodiscs and supported lipid bilayers at the silicon-water interface. We show that lipid and polymer exchange occurs in all nanodiscs tested, although the rate and extent differs between different nanodisc types. Furthermore, we observe adsorption of nanodiscs to the supported lipid bilayer for one nanodisc system which used a polymer made using reversible addition-fragmentation chain transfer polymerization. These results have important implications in applications of polymer-stabilized nanodiscs, such as in the fabrication of solid-supported films containing membrane proteins.
- Published
- 2020