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Your search keyword '"Mäkilä, E."' showing total 2 results
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2 results on '"Mäkilä, E."'

1. Antibacterial properties of nitric oxide-releasing porous silicon nanoparticles

Author

M. Hasanzadeh Kafshgari, Nicolas H. Voelcker, Alex Cavallaro, Jarno Salonen, Ermei Mäkilä, Krasimir Vasilev, Bahman Delalat, Frances J. Harding, Kafshgari, M Hasanzadeh, Delalat, B, Harding, FJ, Cavallaro, A, Mäkilä, E, Salonen, J, Vasilev, K, and Voelcker, NH

Subjects
Materials science, efficacy, Biomedical Engineering, Nanoparticle, Nanotechnology, 02 engineering and technology, Bacterial growth, Conjugated system, 010402 general chemistry, Porous silicon, medicine.disease_cause, 01 natural sciences, Nitric oxide, chemistry.chemical_compound, medicine, silver, General Materials Science, bacteria, Escherichia coli, ta114, biology, General Chemistry, General Medicine, 021001 nanoscience & nanotechnology, biology.organism_classification, Ascorbic acid, 0104 chemical sciences, antibiotic-resistance, chemistry, 0210 nano-technology, Bacteria, wound care, Nuclear chemistry
Abstract

In this study, the antibacterial efficacy of NO-releasing porous silicon nanoparticles (pSiNPs) is reported. NO-releasing pSiNPs were produced via the conjugation of S-nitrosothiol (SNO) and S-nitrosoglutathione (GSNO) donors to the nanoparticle surfaces. The release of the conjugated NO caused by the decomposition of the conjugated SNO and GSNO was boosted in the presence of ascorbic acid. The released NO was bactericidal to Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli), and eliminated bacterial growth within 2 h of incubation without compromising the viability of mammalian cells. These results demonstrate the advantages of NO-releasing pSiNPs for antibacterial applications, for example, in chronic wound treatment. Refereed/Peer-reviewed

Published
2020

2. Small interfering RNA delivery by polyethylenimine-functionalised porous silicon nanoparticles

Author

Bahman Delalat, Ermei Mäkilä, Frances J. Harding, M. Hasanzadeh Kafshgari, Jarno Salonen, M. Alnakhli, Bryone J. Kuss, Sinoula Apostolou, Nicolas H. Voelcker, Hasanzadeh, Kafshgari Morteza, Alnakhli, M, Delalat, Bahman, Apostolou, S, Harding, Fran, Mäkilä, E, Salonen, J, Kuss, B, and Voelcker, Nico

Subjects
Small interfering RNA, Silicon, Biomedical Engineering, Nanoparticle, Cell membrane, chemistry.chemical_compound, Cell Line, Tumor, medicine, Humans, Polyethyleneimine, General Materials Science, RNA, Small Interfering, Cytotoxicity, Polyethylenimine, ta114, Chemistry, Cell growth, technology, industry, and agriculture, RNA, Molecular biology, silicon nanoparticles, porous silicon, medicine.anatomical_structure, Cell culture, Biophysics, cytotoxicity, Nanoparticles, Multidrug Resistance-Associated Proteins, Glioblastoma, Porosity
Abstract

In this study, thermally hydrocarbonised porous silicon nanoparticles (THCpSiNPs) capped with polyethylenimine (PEI) were fabricated, and their potential for small interfering RNA (siRNA) delivery was investigated in an in vitro glioblastoma model. PEI coating following siRNA loading enhanced the sustained release of siRNA, and suppressed burst release effects. The positively-charged surface improved the internalisation of the nanoparticles across the cell membrane. THCpSiNP-mediated siRNA delivery reduced mRNA expression of the MRP1 gene, linked to the resistence of glioblastoma to chemotherapy, by 63% and reduced MRP1-protein levels by 70%. MRP1 siRNA loaded nanoparticles did not induce cytotoxicity in glioblastoma cells, but markedly reduced cell proliferation. In summary, the results demonstrated that non-cytotoxic cationic THCpSiNPs are promising vehicles for therapeutic siRNA delivery. Refereed/Peer-reviewed

Published
2015

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