19 results on '"Lucas Kich Grun"'
Search Results
2. Bioinformatic Analysis of Human Cumulus Cells to Unravel Cellular's Processes that Could Be Used to Establish Oocyte Quality Biomarkers with Clinical Application
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Lucia von Mengden, Marco Antônio De Bastiani, Lucas Kich Grun, Florencia Barbé-Tuana, Tom Adriaenssens, Johan Smitz, Leticia Schmidt Arruda, Carlos Alberto Link, Fábio Klamt, Pathology/molecular and cellular medicine, Clinical Biology, and Follicle Biology
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Bioinformatic ,blastocyst formation ,Cumulus oophorus cells ,IVF ,Obstetrics and Gynecology ,Genetics(clinical) ,oocyte quality ,Functional enrichment analysis ,reproductive medicine - Abstract
Metadata analysis of public microarray datasets using bioinformatics tools has been successfully used in several biomedical fields in the search for biomarkers. In reproductive science, there is an urgent need for the establishment of oocyte quality biomarkers that could be used in the clinical environment to increase the chances of successful outcomes in treatment cycles. Adaptive cellular processes observed in cumulus oophorus cells reflect the conditions of the follicular microenvironment and may thus bring relevant information of oocyte's conditions. Here we analyzed human cumulus cells gene expression datasets in search of predictors of oocyte quality, a strategy which uncovered several cellular processes positively and negatively associated with embryo development and pregnancy potential. Secondly, the expression levels of genes that were present in the majority of processes observed were validated in house with clinical samples. Our data confirmed the association of the selected biomarkers with blastocyst formation and pregnancy potential rates, independently of patients' clinical characteristics such as diagnosis, age, BMI, and stimulation protocol applied. This study shows that bioinformatic analysis of cellular processes can be successfully used to elucidate possible oocyte quality biomarkers. Our data reinforces the need to consider clinical characteristics of patients when selecting relevant biomarkers to be used in the clinical environment and suggests a combination of positive (PTGS2) and negative (CYPB1) quality biomarkers as a robust strategy for a complementary oocyte selection tool, potentially increasing assisted reproduction success rates. Also, GPX4 expression as pregnancy potential biomarker is indicated here as a possibility for further investigations.
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- 2022
3. Telomere length and epigenetic age acceleration in adolescents with anxiety disorders
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Gisele Gus Manfro, Andressa Bortoluzzi, Patricia Lavandoski, Fátima Theresinha Costa Rodrigues Guma, Angelica de Baumont, Maurício Scopel Hoffmann, Giovanni Abrahão Salum, Luciano Santos Pinto Guimarães, Florencia María Barbé-Tuana, Gabriel Rodrigo Fries, and Lucas Kich Grun
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Male ,0301 basic medicine ,Aging ,medicine.medical_specialty ,Adolescent ,Chronic anxiety ,Science ,Real-Time Polymerase Chain Reaction ,Article ,Epigenesis, Genetic ,03 medical and health sciences ,Medical research ,0302 clinical medicine ,Internal medicine ,Humans ,Medicine ,Epigenetics in the nervous system ,Epigenetics ,Multidisciplinary ,Molecular medicine ,business.industry ,HQ The family. Marriage. Woman ,Development of the nervous system ,DNA Methylation ,Telomere ,medicine.disease ,Anxiety Disorders ,Mental health ,030104 developmental biology ,Case-Control Studies ,Cohort ,Anxiety ,Female ,medicine.symptom ,business ,RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry ,Biomarkers ,030217 neurology & neurosurgery ,Anxiety disorder ,Psychopathology - Abstract
Evidence on the relationship between genetics and mental health are flourishing. However, few studies are evaluating early biomarkers that might link genes, environment, and psychopathology. We aimed to study telomere length (TL) and epigenetic age acceleration (AA) in a cohort of adolescents with and without anxiety disorders (N = 234). We evaluated a representative subsample of participants at baseline and after 5 years (n = 76) and categorized them according to their anxiety disorder diagnosis at both time points: (1) control group (no anxiety disorder, n = 18), (2) variable group (anxiety disorder in one evaluation, n = 38), and (3) persistent group (anxiety disorder at both time points, n = 20). We assessed relative mean TL by real-time quantitative PCR and DNA methylation by Infinium HumanMethylation450 BeadChip. We calculated AA using the Horvath age estimation algorithm and analyzed differences among groups using generalized linear mixed models. The persistent group of anxiety disorder did not change TL over time (p = 0.495). The variable group had higher baseline TL (p = 0.003) but no accelerated TL erosion in comparison to the non-anxiety control group (p = 0.053). Furthermore, there were no differences in AA among groups over time. Our findings suggest that adolescents with chronic anxiety did not change telomere length over time, which could be related to a delay in neuronal development in this period of life.
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- 2021
4. Cholinergic Differentiation of Human Neuroblastoma SH-SY5Y Cell Line and Its Potential Use as an In vitro Model for Alzheimer’s Disease Studies
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Richard B. Parsons, Liana Marengo de Medeiros, Eduardo Pacheco Rico, Lucas Kich Grun, Mauro A. A. Castro, Fábio Klamt, Bianca Pfaffenseller, Bianca Wollenhaupt-Aguiar, Eduardo R. Zimmer, Florencia María Barbé-Tuana, Patrícia Schonhofen, and Marco Antônio De Bastiani
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0301 basic medicine ,SH-SY5Y ,Neurite ,Neuroscience (miscellaneous) ,Tretinoin ,Models, Biological ,Neuroblastoma ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,0302 clinical medicine ,Alzheimer Disease ,Cell Line, Tumor ,Neurites ,medicine ,Humans ,Cholinergic neuron ,Cognitive decline ,Brain-Derived Neurotrophic Factor ,Neurotoxicity ,Cell Differentiation ,medicine.disease ,Acetylcholinesterase ,Choline acetyltransferase ,Cholinergic Neurons ,Cell biology ,030104 developmental biology ,Gene Expression Regulation ,Neurology ,chemistry ,Synapses ,Cholinergic ,Biomarkers ,030217 neurology & neurosurgery ,Signal Transduction - Abstract
Cholinergic transmission is critical to high-order brain functions such as memory, learning, and attention. Alzheimer's disease (AD) is characterized by cognitive decline associated with a specific degeneration of cholinergic neurons. No effective treatment to prevent or reverse the symptoms is known. Part of this might be due to the lack of in vitro models that effectively mimic the relevant features of AD. Here, we describe the characterization of an AD in vitro model using the SH-SY5Y cell line. Exponentially growing cells were maintained in DMEM/F12 medium and differentiation was triggered by the combination of retinoic acid (RA) and BDNF. Both acetylcholinesterase (AChE) and choline acetyltransferase (ChAT) enzymatic activities and immunocontent were determined. For mimicking tau and amyloid-β pathology, RA + BDNF-differentiated cells were challenged with okadaic acid (OA) or soluble oligomers of amyloid-β (AβOs) and neurotoxicity was evaluated. RA + BDNF-induced differentiation resulted in remarkable neuronal morphology alterations characterized by increased neurite density. Enhanced expression and enzymatic activities of cholinergic markers were observed compared to RA-differentiation only. Combination of sublethal doses of AβOs and OA resulted in decreased neurite densities, an in vitro marker of synaptopathy. Challenging RA + BDNF-differentiated SH-SY5Y cells with the combination of sublethal doses of OA and AβO, without causing considerable decrease of cell viability, provides an in vitro model which mimics the early-stage pathophysiology of cholinergic neurons affected by AD.
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- 2019
5. Guarana (Paullinia cupanaMart.) alters gut microbiota and modulates redox status, partially via caffeine in Wistar rats
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Lucas Kich Grun, Henrique Mautone Gomes, Maurilio da Silva Morrone, Adriana Giongo, José Cláudio Fonseca Moreira, Alexandre Kleber Silveira, Rafael Rodrigues de Oliveira, Karla Suzana Moresco, Leandro de Mattos Pereira, and Daniel Pens Gelain
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0301 basic medicine ,Pharmacology ,chemistry.chemical_classification ,Antioxidant ,biology ,medicine.medical_treatment ,Alkaloid ,Gut flora ,biology.organism_classification ,medicine.disease_cause ,food.food ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Enzyme ,food ,chemistry ,Polyphenol ,medicine ,Paullinia cupana ,Caffeine ,Oxidative stress - Abstract
Microbiota alterations are observed in pathological conditions, and their regulation is a subject of great interest. Gut microbes are affected by diet, and plant polyphenols may have positive effect on gut microbiota; however, plant-derived extracts may have toxic effects. Guarana (Paullinia cupana Mart.) is a nontraditional medicinal plant applied worldwide. Guarana yields an alkaloid and polyphenol-rich seed with antimicrobial, antioxidant, and anti-inflammatory properties, where caffeine is the major compound. We evaluated the effects of guarana seed powder (GSP) and purified caffeine on gut microbial composition and redox and inflammatory parameters in Wistar rats after 21 days of treatment. Fecal microbiota was analyzed utilizing 16S rDNA sequencing. Antioxidant enzymes activities from liver, kidney, and colon, as well as oxidative damage markers, were evaluated. Total nonenzymatic antioxidant potential was also evaluated. Microbiota was altered by both treatments, GSP and caffeine, without loss of diversity. In the liver, the kidney, and the colon, we observed a decrease in the antioxidant enzymes activities in the GSP group with no increase in the expression of oxidative damage markers, although some enzymes were also regulated by caffeine. Taken together, these results suggested that GSP ameliorates redox parameters but negatively affected gut microbiota, partially via caffeine.
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- 2018
6. Human Chromosome Telomeres
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Mark E. Shiburah, Stephany Cacete Paiva, Vinícius Pierdoná, Beatriz C. D. de Oliveira, Florencia María Barbé-Tuana, Vitor Luiz Carvalho da Silva, Verônica Silva Fontes, Edna Gicela Ortiz Morea, Lucas Kich Grun, and Maria Isabel Nogueira Cano
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Telomerase ,Progeria ,Somatic cell ,Cancer cell ,medicine ,Context (language use) ,Cell cycle ,Biology ,medicine.disease ,Reverse transcriptase ,Cell biology ,Telomere - Abstract
Telomeres are specialized sequences at the end of linear chromosomes. Its conserved structure and function among eukaryotic cells suggest important evolutionary functions. Telomere dynamics play major roles in chromosomal integrity, stability, cellular replication and aging, performing crucial genome protective functions. In the majority of somatic cells, telomeres shorten in each round of cell replication. Whereas short telomeres are a trigger for apoptosis, accelerated attrition is a hallmark of aging, present in senescent and tumoral cells. Compensation for erosion in germinal and progenitor cells is accomplished by the telomerase enzyme, composed of a catalytic (TERT) and a RNA template (TR) subunits. Telomerase activity is tightly controlled with reactivation central for tumoral transformation. Clinical evidence suggests that telomeres’ shortening that causally contributes to the establishment of specific progeria syndromes phenotypes are telomeropathies. In other cases, aging is accompanied by an abrupt telomere shortening in the context of chronic diseases, proposing telomeres length as an important biological pace marker for progression of various pathologies and aging. Because cancer cells reactivate TERT to compensate for telomeric loss, in the last decades, telomerase and the telomeres biology field have been subject to intense research in search for therapeutical targets for cancer. This chapter mainly focuses on basic telomere description and synthesis accomplished by the reverse transcriptase telomerase and its regulation. A final section addresses the understanding of telomeres in health and its contribution to cancer with therapeutic potentials for targeted inhibition.
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- 2021
7. Shorter telomeres in children with severe asthma, an indicative of accelerated aging
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Leonardo Araújo Pinto, Marcus Herbert Jones, Renato T. Stein, Fátima Theresinha Costa Rodrigues Guma, Mariana Migliorini Parisi, Paulo Márcio Condessa Pitrez, Florencia María Barbé-Tuana, Frederico Orlando Friedrich, Vinícius Pierdoná, and Lucas Kich Grun
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Senescence ,Chemokine CCL11 ,Male ,severe asthma ,medicine.medical_specialty ,Chemokine ,Aging ,senescence ,Adolescent ,Internal medicine ,Eosinophilic ,medicine ,telomere length ,Humans ,Respiratory system ,Child ,CCL11 ,Telomere Shortening ,Asthma ,biology ,business.industry ,Cell Biology ,medicine.disease ,Accelerated aging ,Telomere ,Endocrinology ,Case-Control Studies ,biology.protein ,Female ,inflammaging ,business ,Research Paper - Abstract
Severe therapy-resistant asthma (STRA) is closely associated with distinct clinical and inflammatory pheno-endotypes, which may contribute to the development of age-related comorbidities. Evidence has demonstrated a contribution of accelerated telomere shortening on the poor prognosis of respiratory diseases in adults. Eotaxin-1 (CCL11) is an important chemokine for eosinophilic recruitment and the progression of asthma. In the last years has also been proposed as an age-promoting factor. This study aimed to investigate the association of relative telomere length (rTL) and eotaxin-1 in asthmatic children. Children aged 8-14 years (n=267) were classified as healthy control (HC, n=126), mild asthma (MA, n=124) or severe therapy-resistant asthma (STRA, n=17). rTL was performed by qPCR from peripheral blood. Eotaxin-1 was quantified by ELISA from fresh-frozen plasma. STRA had shorter telomeres compared to HC (p=0.02) and MA (p=0.006). Eotaxin-1 levels were up-regulated in STRA [median; IQR25-75)] [(1,190 pg/mL; 108-2,510)] compared to MA [(638 pg/mL; 134-1,460)] (p=0.03) or HC [(627 pg/mL; 108-1,750)] (p
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- 2020
8. Methoxyeugenol regulates the p53/p21 pathway and suppresses human endometrial cancer cell proliferation
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Bruna Pasqualotto Costa, Florencia María Barbé-Tuana, Lucas Kich Grun, Gisele Branchini, Krist Helen Antunes Fernandes, Géssica Luana Antunes, Marcella Tornquist Nassr, Fernanda Bordignon Nunes, Fernando Mendonça Diz, and Jarbas Rodrigues de Oliveira
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Cyclin-Dependent Kinase Inhibitor p21 ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,Drug Discovery ,Eugenol ,medicine ,Humans ,MTT assay ,Cytotoxicity ,030304 developmental biology ,Cell Proliferation ,Pharmacology ,chemistry.chemical_classification ,0303 health sciences ,Reactive oxygen species ,medicine.diagnostic_test ,Cell growth ,Cancer ,medicine.disease ,Antineoplastic Agents, Phytogenic ,Endometrial Neoplasms ,Mitochondria ,Gene Expression Regulation, Neoplastic ,chemistry ,Apoptosis ,Cell culture ,030220 oncology & carcinogenesis ,Cancer research ,Female ,Tumor Suppressor Protein p53 ,Reactive Oxygen Species ,Signal Transduction - Abstract
Ethnopharmacological relevance Plant-derived compounds are a reservoir of natural chemicals and can act as drug precursors or prototypes and pharmacological probes. Methoxyeugenol is a natural compound found in plant extracts, such as nutmeg (Myristica fragrans), and it presents anthelmintic, antimicrobial, anti-inflammatory activities. Recently, interest in the anticancer activity of plant extracts is increasing and the therapeutic activity of methoxyeugenol against cancer has not yet been explored. Aim of the study The present study aimed to evaluate the cancer-suppressive role and the molecular signaling pathways of methoxyeugenol in human endometrial cancer (Ishikawa) cell line. Materials and methods Proliferation, viability, and cell toxicity were assessed by direct counting, MTT assay, and LDH enzyme release assay, respectively. Antiproliferative effect were evaluated by nuclear morphological changes along with the cellular mechanisms of apoptosis and senescence by flow cytometry. The underlying molecular and cellular mechanisms were investigated by RT-qPCR, reactive oxygen species (ROS) levels, mitochondrial dysfunction, and proliferative capacity. Results and conclusions Methoxyeugenol treatment significantly inhibited the proliferation and viability of Ishikawa cells. Probably triggered by the higher ROS levels and mitochondrial dysfunction, the gene expression of p53 and p21 increased and the gene expression of CDK4/6 decreased in response to the methoxyeugenol treatment. The rise in nuclear size and acidic vesicular organelles corroborate with the initial senescence-inducing signals in Ishikawa cells treated with methoxyeugenol. The antiproliferative effect was not related to cytotoxicity and proved to effectively reduce the proliferative capacity of endometrial cancer cells even after treatment withdrawal. These results demonstrated that methoxyeugenol has a promising anticancer effect against endometrial cancer by rising ROS levels, triggering mitochondrial instability, and modulating cell signaling pathways leading to an inhibition of cell proliferation.
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- 2020
9. Arundic acid (ONO-2526) inhibits stimulated-S100B secretion in inflammatory conditions
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Lucas Kich Grun, Juliana de Lima Cordeiro, Florencia María Barbé-Tuana, Carlos Alberto Gonçalves, Marina Concli Leite, J.D. Neves, Carlos Alexandre Netto, Adriana Fernanda Kuckartz Vizuete, and Marina Pedra Seady
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Lipopolysaccharides ,Male ,0301 basic medicine ,medicine.medical_specialty ,Anti-Inflammatory Agents ,S100 Calcium Binding Protein beta Subunit ,Hippocampal formation ,Hippocampus ,Neuroprotection ,Proinflammatory cytokine ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Extracellular ,Animals ,Secretion ,Rats, Wistar ,Cells, Cultured ,Neuroinflammation ,Tumor Necrosis Factor-alpha ,Chemistry ,General Neuroscience ,Rats ,Neuroprotective Agents ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Astrocytes ,Tumor necrosis factor alpha ,Caprylates ,030217 neurology & neurosurgery ,Astrocyte - Abstract
Astrocytes respond to injury by modifying the expression profile of several proteins, including the S100 calcium-binding protein B (S100B), assumed to be a marker as well as a mediator of brain injury. AA is an inhibitor of S100B synthesis and plays a protective role in different models of brain injury, as decreases in S100B expression cause decreases in extracellular S100B. However, S100B mRNA expression, S100B protein content and S100B secretion do not always occur in association; as such, we herein investigated the effect of AA on S100B secretion, using different approaches with three stimulating conditions for S100B secretion, namely, low potassium medium, TNF-α (in hippocampal slices) and LPS exposure (in astrocyte cultures). Our data indicate that AA directly affects S100B secretion, indicating that the extracellular levels of this astroglial protein may be mediating the action of this compound. More importantly, AA had no effect on basal S100B secretion, but inhibited stimulated S100B secretion (stimulated either by the proinflammatory molecules, LPS or TNF-α, or by low potassium medium). Data from hippocampal slices that were directly exposed to AA, or from animals that received the acid by intracerebroventricular infusion, contribute to understanding its neuroprotective effect.
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- 2021
10. Immunosenescence Induced by Plasma from Individuals with Obesity Caused Cell Signaling Dysfunction and Inflammation
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Alexandre Vontobel Padoin, Florencia María Barbé-Tuana, Letícia Biscaino Alves, Mariana Migliorini Parisi, Marcus Herbert Jones, Lucas Kich Grun, Ivi Juliana Bristot, Fábio Klamt, Rita Mattiello, Patricia Lavandoski, Fátima Theresinha Costa Rodrigues Guma, and Cláudio Corá Mottin
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0301 basic medicine ,medicine.medical_specialty ,Nutrition and Dietetics ,business.industry ,Endocrinology, Diabetes and Metabolism ,CD14 ,Medicine (miscellaneous) ,Inflammation ,Immunosenescence ,Systemic inflammation ,Peripheral blood mononuclear cell ,Proinflammatory cytokine ,03 medical and health sciences ,030104 developmental biology ,Endocrinology ,Internal medicine ,Immunology ,Medicine ,medicine.symptom ,business ,CD80 ,CD8 - Abstract
Objective To evaluate the consequences of plasma from individuals with obesity on parameters associated with immunosenescence in unrelated healthy peripheral blood mononuclear cells (PBMC). Methods Freshly isolated PBMC were incubated in media supplemented with 10% of plasma from individuals with obesity or control subjects for the first 4 hours of 24 to 120 hours of culture. Results Plasma from individuals with obesity modulated the phenotype of healthy PBMC, leading to a higher rate of apoptosis, lower amounts of phospho-γH2AX and -p53, and mitochondrial dysfunction. After 120 hours, there was a higher secretion of inflammatory cytokines IL-1β and IL-8. CD8+ T lymphocytes presented decreased expression of CD28, which is associated with the immunosenescent phenotype. CD14+ macrophages showed increased expression of CD80 and CD206, suggesting a modulation in the activation of macrophages. Conclusions These results demonstrate that chronic systemic inflammation observed in obesity induces dysfunctional features in PBMC that are consistent with premature immunosenescence.
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- 2017
11. Association between shorter telomeres’ length and Eotaxin-1 in children with severe asthma, an indicative of accelerated aging
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Renato T. Stein, Paulo Márcio Pitrez, Fátima Theresinha Costa Rodrigues Guma, Leonardo Araújo Pinto, Mariana Migliorini Parisi, Florencia María Barbé-Tuana, Lucas Kich Grun, Vinícius Pierdoná, and Marcus Herbert Jones
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Eotaxin ,COPD ,medicine.medical_specialty ,business.industry ,Severe asthma ,respiratory system ,medicine.disease ,Accelerated aging ,Peripheral blood ,Telomere ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,030228 respiratory system ,Internal medicine ,medicine ,030212 general & internal medicine ,business ,CCL11 ,Asthma - Abstract
Introduction: Severe therapy-resistant asthma (STRA) is associated with a range of pheno-endotypes and adults with STRA have higher risk for developing comorbidities. Studies investigating telomere length (TL) in adults have demonstrated telomere shortening in IPF, COPD and asthma. Eotaxin-1 (CCL11), an important modulator for eosinophilic function and chemo-attraction has also been proposed an aging factor. Aims: To investigate whether differences exist in TL and CCL11 on peripheral blood cells between age-matched severe and mild asthmatic (MA) children and healthy control (HC) subjects. Methods: Relative telomere length (T/S) was quantified by qPCR (peripheral blood) from 124 children with MA, 17 STRA and 73 age-matched HC. CCL11 was quantified by ELISA. Results: T/S analyses indicated that STRA group (median 0.818, IQR25-27 0.496–1.041) had significantly shorter telomeres when compared to MA (1.083 (0.732–1.582), P=0.043). No difference was observed between T/S ratio of MA or STRA and HC (0.998, (0.630–1.740)). Higher levels of CCL11 were found among children within STRA group (1,119 pg/mL (554 - 1,401)) when compared to MA (675 (388 - 1,015) or HC children (626 (256 - 922), P=0.023) with ordered differences among classes (P=0.021). T/S ratio of STRA was inversely correlated with CCL11 (r=-0.573, P=0.011). Conclusions: This is the first report showing a decrease in TL in children with STRA and associated with up-regulation of CCL11. This study adds new evidence to the theory of accelerated aging suggesting that, telomeres attrition and CCL11, could prematurely contribute to a senescent phenotype associated with risk for appearance of comorbidities
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- 2019
12. Telomere length in subjects with schizophrenia, their unaffected siblings and healthy controls: Evidence of accelerated aging
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David Freitas de Lucena, Letícia Sanguinetti Czepielewski, Florencia María Barbé-Tuana, Danielle Silveira Macêdo, Eduarda Dias da Rosa, Lucas Kich Grun, Bruna Panizzutti, Raffael Massuda, and Clarissa Severino Gama
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Adult ,Male ,Aging ,medicine.medical_specialty ,Adolescent ,Endophenotypes ,Body Mass Index ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Interview, Psychological ,medicine ,Humans ,Bipolar disorder ,Young adult ,Pathological ,Telomere Shortening ,Biological Psychiatry ,Psychiatric Status Rating Scales ,Genetics ,Analysis of Variance ,Siblings ,Middle Aged ,Telomere ,medicine.disease ,Pathophysiology ,030227 psychiatry ,Psychiatry and Mental health ,Endocrinology ,Schizophrenia ,Endophenotype ,Educational Status ,Biomarker (medicine) ,Female ,Analysis of variance ,Psychology ,030217 neurology & neurosurgery ,Antipsychotic Agents - Abstract
Schizophrenia (SZ) is associated with broad burden. The clinical manifestations of SZ are related to pathophysiological alterations similar to what is seen in normal aging. Our aim was to evaluate the differences in telomere length (TL), a biomarker of cellular aging, in subjects with SZ (n=36), unaffected siblings (SB, n=36) and healthy controls (HC, n=47). SZ had shorter TL compared to HC, but no difference was found in SB comparing to SZ. These findings indicate that a pathological accelerated aging profile could be present in the course of SZ and further studies are needed to confirm TL as potential endophenotype, especially in at risk populations.
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- 2016
13. Retinoic acid downregulates thiol antioxidant defences and homologous recombination while promotes A549 cells sensitization to cisplatin
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Daniel Pens Gelain, Nauana Somensi, Helen Tais da Rosa, José Cláudio Fonseca Moreira, Vitor de Miranda Ramos, Lucas Kich Grun, Amanda de Fraga Dias, Diana Carolina Rostirolla, Florencia María Barbé-Tuana, Fabrício Figueiró, and Juciano Gasparotto
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0301 basic medicine ,Lung Neoplasms ,NF-E2-Related Factor 2 ,DNA repair ,Retinoic acid ,Apoptosis ,Tretinoin ,Antioxidants ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Autophagy ,medicine ,Humans ,Sulfhydryl Compounds ,Homologous Recombination ,Cell Proliferation ,Cisplatin ,A549 cell ,Chemistry ,Cell Biology ,respiratory system ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,A549 Cells ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Homologous recombination ,medicine.drug - Abstract
Recent studies have investigated the use of retinoic acid (RA) molecule in combined chemotherapies to cancer cells as an attempt to increase treatment efficiency and circumvent cell resistance. Positive results were obtained in clinical trials from lung cancer patients treated with RA and cisplatin. Meanwhile, the signalling process that results from the interaction of both molecules remains unclear. One of the pathways that RA is able to modulate is the activity of NRF2 transcription factor, which is highly associated with tumour progression and resistance. Therefore, the aim of this work was to investigate molecular mechanism of RA and cisplatin co-treatment in A549 cells, focusing in NRF2 pathway. To this end, we investigated NRF2 and NRF2-target genes expression, cellular redox status, cisplatin-induced apoptosis, autophagy and DNA repair through homologous recombination. RA demonstrated to have an inhibitory effect over NRF2 activation, which regulates the expression of thiol antioxidants enzymes. Moreover, RA increased reactive species production associated with increased oxidation of thiol groups within the cells. The expression of proteins associated with DNA repair through homologous recombination was also suppressed by RA pre-treatment. All combined, these effects appear to create a more sensitive cellular environment to cisplatin treatment, increasing apoptosis frequency. Interestingly, autophagy was also increased by combination therapy, suggesting a resistance mechanism by A549 cells. In conclusion, these results provided new information about molecular mechanisms of RA and cisplatin treatment contributing to chemotherapy optimization.
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- 2019
14. Guarana (Paullinia cupana Mart.) alters gut microbiota and modulates redox status, partially via caffeine in Wistar rats
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Alexandre, Kleber Silveira, Karla Suzana, Moresco, Henrique, Mautone Gomes, Maurílio, da Silva Morrone, Lucas, Kich Grun, Daniel, Pens Gelain, Leandro, de Mattos Pereira, Adriana, Giongo, Rafael, Rodrigues De Oliveira, and José Cláudio, Fonseca Moreira
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Male ,Plants, Medicinal ,Plant Extracts ,Antioxidants ,Gastrointestinal Microbiome ,Rats ,Oxidative Stress ,Theophylline ,Caffeine ,Seeds ,Paullinia ,Animals ,Dysbiosis ,Theobromine ,Rats, Wistar ,Oxidation-Reduction - Abstract
Microbiota alterations are observed in pathological conditions, and their regulation is a subject of great interest. Gut microbes are affected by diet, and plant polyphenols may have positive effect on gut microbiota; however, plant-derived extracts may have toxic effects. Guarana (Paullinia cupana Mart.) is a nontraditional medicinal plant applied worldwide. Guarana yields an alkaloid and polyphenol-rich seed with antimicrobial, antioxidant, and anti-inflammatory properties, where caffeine is the major compound. We evaluated the effects of guarana seed powder (GSP) and purified caffeine on gut microbial composition and redox and inflammatory parameters in Wistar rats after 21 days of treatment. Fecal microbiota was analyzed utilizing 16S rDNA sequencing. Antioxidant enzymes activities from liver, kidney, and colon, as well as oxidative damage markers, were evaluated. Total nonenzymatic antioxidant potential was also evaluated. Microbiota was altered by both treatments, GSP and caffeine, without loss of diversity. In the liver, the kidney, and the colon, we observed a decrease in the antioxidant enzymes activities in the GSP group with no increase in the expression of oxidative damage markers, although some enzymes were also regulated by caffeine. Taken together, these results suggested that GSP ameliorates redox parameters but negatively affected gut microbiota, partially via caffeine.
- Published
- 2018
15. Effect of obesity on telomere length: Systematic review and meta-analysis
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Renato T. Stein, Rita Mattiello, Joao Mário Mazzola, Marcus Herbert Jones, Lucas Kich Grun, Helen Zatti, Fátima Theresinha Costa Rodrigues Guma, Florencia María Barbé-Tuana, Eduardo Mundstock, Fernanda Mattos Louzada, Matias Epifanio, and Edgar E. Sarria
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Gerontology ,Nutrition and Dietetics ,business.industry ,Endocrinology, Diabetes and Metabolism ,MEDLINE ,Medicine (miscellaneous) ,Cochrane Library ,Overweight ,Bioinformatics ,medicine.disease ,Obesity ,Telomere ,Endocrinology ,Telomere Homeostasis ,Meta-analysis ,medicine ,medicine.symptom ,Negative correlation ,business - Abstract
Objective The main objective of this systematic review is to assess the effects of obesity on telomere length. Methods The following databases were searched: MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials (CENTRAL, The Cochrane Library), LILACS, SPORTdiscus, and Web of Science from inception to August 2014. The search was performed using the following combinations of terms: telomere AND “overweight” OR “obesity” OR “adiposity,” without language restriction. Results Sixty-three original studies were included in this systematic review, comprising 119,439 subjects. Thirty-nine studies showed either weak or moderate correlation between obesity and telomere length; however, they showed an important heterogeneity. Conclusions There is a tendency toward demonstrating negative correlation between obesity and telomere length. The selected studies showed weak to moderate correlation for the main search, and there was an important heterogeneity. For this reason, the causal relationship of obesity and telomere length remains open. Additional controlled longitudinal studies are needed to investigate this issue.
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- 2015
16. Proceedings of the 23rd Paediatric Rheumatology European Society Congress: part two
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David Kern, Masato Yashiro, Gerd Horneff, Ana P. Sakamoto, Berent J. Prakken, Paula Vähäsalo, Juergen Brunner, Ezgi H. Baris, Helen McCarthy, Janet E. McDonagh, A. Grom, Adriana Albu, Lenka Linkova, I. Nikishina, Daniel Álvarez de la Sierra, Bruno Papia, Peggy Lee, Luisa Giannone, Tobias Schwarz, Mekibib Altaye, Margarita Onoufriou, Tatiana Sleptsova, N. Ruperto, O Thana, A. Baheti, Ilonka Orbán, Kai Lehmberg, F. Zulian, Helga Sanner, Karin Palmblad, Kousuke Shabana, Sebastiaan Vastert, Marta Rusmini, Olga Vougiouka, Dirk Holzinger, D. Shaikhani, Shouichi Ohga, Ismail Dursun Dursun, Claire T Deakin, Ingrid Herta Rotstein Grein, Maria Trachana, Ariane Klein, Eugenia Enriquez, Angelo Ravelli, Paul A. Brogan, L.S. Nazarova, Laila Al Shaqshi, Paulina Vele, Liana Guerra, Antonia Pascarella, Jelena Vojinovic, Juliana Molina, Kjell Tullus, S. Rodionovskaya, Chris Scott, A. N. Olivieri, Cliff Taggart, Clare Heard, Ricardo Pujol Borrell, Jens Klotsche, Grendel Burrell, Oriany L. Pereira, Silvia Giliani, Sandra Pereira, Jennifer Horonjeff, Beth A. Mueller, Lyudmyla M. Byelyaeva, Sergio Tufik, Carlo Agostoni, Valentina Muratore, Rostislav M. Filonovich, Fiona Hawke, Virginia Messia, Bo Magnusson, Kerry West, Sara Murias, Mustafa Doğan, Hafize Emine Sönmez, Annet van Royen-Kerkhof, K. Minden, Raquel Campanilho-Marques, Reem Abdawani, Maria Ceci, Maria Ekelund, Seza Ozen, Ratna Puri, Girolamo Luppino, Shannon Carr, Rita A. Amorim, K Kobrová, Rachael D. Wright, Chantal Job-Deslandre, Daniel J. Lovell, Jorge Kalil, Yi-jin Gao, Kubra Ozturk, Fulvio Parentin, Ursula Fearon, Frank Weller-Heinemann, Elizabeth Ang, Charles A Mebus, Andrea Superti-Furga, Alina Ferster, Rikard Wicksell, Mohammadreza Modaressi, F La Torre, Ela Tarakci, Wendy Thomson, Giorgia Malighetti, Antonio Eleuteri, Helena K. Khrustaleva, Alan Easton, Alexander Mushkin, Sara Marsal Barril, Erkan Demirkaya, Florence Kanakoudi-Tsakalidou, Diana Ekdawy, Lana Tambić Bukovac, Suvi Peltoniemi, Nur Arslan, Hermine I. Brunner, Tim Rapley, Donatella Vairo, Kirill Savostyanov, Fumiko Okazaki, Rachel Corkhill, Tufan Kutlu, MG Alpigiani, Fabio Fernandes Morato Castro, Juliana Farhat, Butsabong Lerkvaleekul, Scolozzi Paolo, Akihiko Saitoh, Jason Dare, Gustavo Rocha, Tatiana V. Viktorova, Riva Brik, Jason Palman, Fabrizia Corona, Susan Nielsen, Johannes Roth, Ma. Theresa M. Collante, Leonardo Oliveira Mendonça, D. Alexeev, Randy Q. Cron, Sriharsha Grevich, Andrea L. Jorgensen, Lúcia Maria de Arruda Campos, Kiran Nistala, Fernando Martins, R. Cimaz, Angela C. Mosquera, Ruy Carrasco, Reyhan Dedeoglu, Giovanni Filocamo, J. Dare, Paula Keskitalo, Ana J. D. F. C. Lichtenfels, Florence Uettwiller, Umberto Conte, Gecilmara Salviato Pileggi, Michal Uher, Mercedes Chan, Sarka Fingerhutova, Anne M. Stevens, Peter Bale, Mikel Alberdi-Saugstrup, Olga L Kopchak, Thomas A. Griffin, Constantin Ailioaie, Clifton Bingham, Ekaterina Alexeeva, Loshinidevi D Bathi, Jane Hurst, AnnaCarin Horne, Laura Muntean, Nermin Uncu, Mara Carraro, C Vargas, Lorenzo Quartulli, Ayşenur Paç Kısaarslan, Angela Mauro, F. Corona, Donato Rigante, Helen J. Lachmann, Ana Cordeiro, Ivan Foeldvari, Faysal Gok, Tatiana Gonzalez, S. S. M. Kamphuis, Hasret Ayyildiz-Civan, Claudia Pastorino, Gleice C. S. Russo, J. B. Kuemmerle-Deschner, Serena Pastore, Nigel Klein, M. Jorini, Tatjana Freye, Maria Tsolia, Philippe Jacqmin, Suzanne M M Verstappen, Syuji Takei, Khalid Hussain, Renzo Marcolongo, Yuichi Yamasaki, Sharmila Jandial, K. Leon, Maria Pia Sormani, T. A. Simon, Mohammed Muzaffer, Catalina Mosquera, Clovis Aa Silva, Zelal Ekinci, Zübeyde Gündüz, Bernd Denecke, Felicitas Bellutti Enders, Despina Eleftheriou, Ishbel MacGregor, Andrew Cant, Luisa Bonafé, Valda Staņēviča, Helen E. Foster, Alberto Tommasini, Nora Bartholomä, Nural Kiper, A. Kardolus, Eloisa Bonfa, Alessandro Consolaro, Lillemor Berntson, Umberto Garagiola, Richard K. Vehe, Vanessa Bugni Miotto e Silva, Chihaya Imai, Kathleen G. Lomax, Brian Best, Barbara Bonafini, M. Toth, D. Rigante, Eiman Abdalla, Leona Prochazkova, Lucy Wedderburn, Lovro Lamot, S. Verazza, Raffaella Carlomagno, Gillian I. Rice, Norm Ilowite, K. de Leeuw, Havva Evrengül, Jerold Jeyaratnam, Andrew Zeft, Andrea Taddio, R. Podda, Samuel Cassidy, Grant S. Schulert, Silvia Rosina, Marija Jelušić, Olivier Gilliaux, Rubén Burgos-Vargas, Mao Mizuta, Akihiro Yachie, Angel Phuti, Antonio Zea Mendoza, Emily Boulter, Zane Dāvidsone, Sofia Torreggiani, Marco Cattalini, Natali W. Gormezano, Fatma Dedeoglu, Hercília Guimarães, A. Insalaco, Andrea Coda, Viktor A. Malievsky, Thomas Zumbrunn, Agostino Nocerino, Ronald Pederson, Katarzyna Kobusinska, Anasuya Hazra, Ananadreia S. Lopes, Elena Campione, Toshiyuki Kitoh, Elena Tsitsami, Henny G. Hotten, Radka Kaneva, R. J. E. M. Dolhain, Ndate Fall, Francesco Licciardi, Deepti Suri, G. D’Angelo, Valentina Seraya, Elżbieta Smolewska, Anastasia Dropol, Ezgi Deniz Batu, Andreas Woerner, Christine Arango, Nadia E. Aikawa, Zoilo Morel, Megan Yuasa, Sandra Ammann, Erbil Unsal, Tomohiro Kubota, Toshitaka Kizawa, Fabrizio De Benedetti, Catherine Laing, Liudmila Rakovska, Yonatan Butbul Aviel, J-Peter Haas, Marta Minute, Christine Alvey, Vasiliko Dermentzoglou, Vania Schinzel, Isree Leelayuwattanakul, Ekim Taskiran, Gabriele Simonini, N. Martin, Nathalie Canham, Nicky Brice, Beatrice Vergara, Ika Birkić, Cengizhan Acikel, Johannes-Peter Haas, Ruth Fritsch, Alisa Vitebskaya, Fatih Yazici, Iva Brito, Nataša Toplak, Veronica Moshe, Gordon J Hendry, Nadia Luca, Deniz Doğru-Ersöz, Marco Matucci-Cerinic, Claudia Toppino, Zoë Johnson, Beatrice Goilav, Siyaram Didel, K. Marzan, Tamar B. Rubinstein, Angela Barnicoat, Peter Nourse, Thita Pacharapakornpong, Adele Civino, Inmaculada Calvo Penades, H. I. Brunner, Massimo Imazio, V. Gerloni, Ayla Kacar, Heinrike Schmeling, Marija Perica, Silvestre García de la Puente, Tadej Avcin, Filipa Oliveira-Ramos, S. Arsenyeva, Phillip J Hashkes, Sabrina Schuller, Adriana Rodriguez Vidal, Kathy de Graaf, Giedre Januskeviciute, M. Kaleda, Lee Dossetter, Jelena Basic, Elena Kaschenko, Erik Sundberg, Gizem Pamuk, Marek Zak, I. Foeldvari, Rachael Quarmby, Marc D. Natter, Antonarakis Gregory, P. A. Brogan, João Eurico Fonseca, Andrea Jorgensen, Ana F. Mourão, Gaurav Gulati, Yelda Bilginer, Banu Acar Celikel, Utako Kaneko, Karen L. Durrant, Alice Grossi, Maurizio Aricò, Ibrahim Al Zakwani, Yildirim Karslioglu, Takuji Murata, Monika Stoll, Maria Teresa Terreri, Ariana Kariminejad, Teresa A. Simon, Laura B Lewandowski, Marina Garcia Prat, Walter G. Ferlin, Albena Telcharova, Giovanni Maria Severini, Judith Wienke, Panagiota Nalbanti, Hakan Poyrazoglu, Athimalaipet V. Ramanan, Manisha Lamba, Z. Guo, J. Bohnsack, Norberto Guelbert, John F. Bohnsack, Lucy R. Wedderburn, Elvira Cannizzaro Schneider, Raul Gutiérrez Suárez, Debra Grech, Yonit Reis, Chris Pruunsild, Amit Rawat, Nienke M. ter Haar, Hiroshi Tamai, Alexander Pushkov, A. De Fanti, Valentina Marzetti, Sheila Weitzman, I. E. M. Bultink, Dilek Keskin, Sania Valieva, Klaus Tenbrock, Ana Luisa Rodriguez-Lozano, Lianne Kearsley-Fleet, Luca Messina, Chiara Gorio, Amra Adrovic, Stephanie J. W. Shoop, Davide Cumetti, Sana Al Zuhbi, Helena Khrustaleva, E. Zirkzee, Elio Castagnola, Clarissa Pilkington, Jingyao Leong, Vitor A. Teixeira, Reinhard Würzner, Sonia Melo Gomes, Orla Killeen, Antony C. Fisher, Sevket Erbil Unsal, Edward M. Behrens, Kristiina Aalto, Rebecca Nicolai, Thomas C. Stock, Luiz Cláudio Danzmann, Y. K. O. Teng, Stephen D. Marks, Fotios Papachristou, Valda Stanevicha, Richard Saffrey, Elizabeth Ralph, Johannes Peter Haas, Mary Slatter, Maria Tsinti, Mehmet Alikasifoglu, Mónica Martínez Gallo, Rayfel Schneider, Rosa Maria Rodrigues Pereira, Maria F. D. A. Giacomin, Alfésio Luís Ferreira Braga, Giulia Camilla Varnier, James McElnay, Jessica Foster, Ingrida Rumba-Rozenfelde, Francesca Minoia, Laurence Goffin, Roger C. Allen, Zehra S. Arıcı, Mette Nørgaard, Alberto Martini, Hofer Michaël, Andreas Eikelberg, Junko Yasumura, Maria José Santos, Tom Wolfs, Ada B. Sinoplu, Natalia Balera Ferreira Pinto, Michael Lang, Umberto Corpora, Hidenobu Kaneyasu, Fiorenzo Gaita, Olga Lomakina, Dimitrina Mihaylova, Gian Luca Erre, Fugen Cullu-Cokugras, Mesut Topdemir, Sriram Krishnaswami, Irina Nikishina, Noortje Groot, S. Pastore, Joke Dehoorne, Paula Estanqueiro, Shafe Fahoum, Francisca Aguiar, Mabel Ladino, Nico M Wulffraat, Jana Franova, Helena Erlandsson-Harris, Denise Pires Marafon, Adrian Liston, Edward H. Schuchman, Jaime C. Branco, Maria Teresa R. Terreri, Radoslava Saraeva, Ulrika Järpemo Nykvist, Maria Cristina Maggio, Kazuko Yamazaki, Lídia Teixeira, Hanquinet Sylviane, Ricardo Yepez, Susan Maillard, Tommy Gerschman, G. Horneff, Anne-Louise Ponsonby, Meredith Riebschleger, Alessandra Alongi, José Melo-Gomes, Iris Haug, Maria De Iorio, Ekaterina Alekseeva, Jan-Inge Henter, Elisa Pisaneschi, H. Kupper, Martina Niewerth, Berta López Montesinos, Shunji Hasegawa, Zahra Hadipour, R. M. Kuester, Silvia Maestroni, Pilar Guarnizo, Antonio Brucato, Tamaki Nakamura, Gustavo Antonio Moreira, Chaim Putterman, A. Hospach, Joost Frenkel, Svetlana O Salugina, A. Ravelli, Pavla Dolezalova, Gunnar L. Olsson, Eva González-Roca, Ellen Dalen Arnstad, Mohammad Alhemairi, Tina Hinnershitz, P. Quartier, Yildirim Karsioglu, Davinder Singh Grewal, Sergio Davì, Gökçen D. Tuğcu, Tomo Nozawa, Emily Robinson, M. C. Maggio, Maria Ballabio, Eleonora Bellucci, Alexei A. Grom, Rosa M. Pereira, Federica Vanoni, Shumpei Yokota, Justine A. Ellis, Helen Bristow, Mohammad-Hassan Moradinejad, Ricardo Russo, Harun Evrengül, Mario Abinun, Laura Carenini, Francesca Santarelli, C. Wallace, Maria Beatriz Fonseca, Timothy Beukelman, Saša Sršen, Véronique Hentgen, Sezgin Sahin, Silvia Zaffarano, Salvatore Albani, Valentin Brodszky, Clovis A. Silva, Graciela Espada, Jana Pachlopnik Schmid, Margaux Gerbaux, Stefano Stagi, Valentina Leone, Brian Leroux, Isabelle Koné-Paut, Gabriella Giancane, Soley Omarsdottir, Benedetta Schiappapietra, Alessandra Carobbio, Ricardo Menendez-Castro, Yoshifumi Kawano, Ozge Erdemli, Monia Lorini, Arjan Boltjes, Ellen Nordal, Carol A. Wallace, Mustafa Çakan, Reni Tzveova, Stefano Lanni, Salah Shokry, Kirsty McLellan, Qiong Wu, Nilay Arman, Adelina Tsakova, Michael W. Beresford, A. Consolaro, Francesca Bovis, Margarita Ganeva, Christoph Kessel, A. Martini, Taichi Kanetaka, Andre Schultz, Flora McErlane, Ronnie Wang, Mojca Zajc Avramovič, Thaschawee Arkachaisri, Boris Huegle, Funda Öztunç, Jane E Munro, Yanick J. Crow, Hiroyuki Wakiguchi, Rita Fonseca, Kim E. Nichols, Mia Glerup, Nils Venhoff, R. Filonovich, Erika Van Nieuwenhove, Nadia Rafiq, Elena Kamenets, Yasuo Nakagishi, Giampietro Farronato, Consuelo Modesto Caballero, Tulay Erkan, Jan Bonhoeffer, Jack Bukowski, Myrthes Toledo Barros, Gladys C. C. Esteves, Mirta Lamot, Rosa Alcobendas, Cláudia Moura, Florencia María Barbé-Tuana, Joo Guan Yeo, Mark Friswell, Yuko Sugita, Ari Shapiro, Nagla Abdelrahman, Phu-Quoc Lê, Kevin Murray, Susanne M Benseler, Anna Monica Bianco, J. Kalabic, Ana Catarina Duarte, Ivan Caiello, Joyce Davidson, Maria Isabel Gonzalez Fernandez, Larisa Zajtseva, Ebun Omoyinmi, Jaime de Inocencio, Dragana Lazarevic, Ritambhra Nada, Claudia Saad-Magalhães C, G. Conti, Andrew Gennery, Caroline Jones, Christophe Lelubre, Brian Rusted, Geneviève Lapeyre, Giulia Zani, Alina Boteanu, Maria T. Terreri, Nataliya Panko, Julia Albrecht, Federica Mongini, Lucio Giordano, Daniela Kaiser, Robert Nelson, Hans-Iko Huppertz, Gonca Keskindemirci, Karla Ištuk, Raffaele Strippoli, Dorota Rowczenio, Emma MacDermott, Roberta Caorsi, Emiliano Marasco, Sandra Sousa, Fatoş Yalçınkaya, Jaanika Ilisson, Yuki Kimura, Marco Turco, Nami Okamoto, Parveen Bhatti, Ekaterina M. Kuchinskaya, Stefano Volpi, Min Wang, Jeffrey M. Craig, M. Bijl, Giusi Prencipe, Fatemeh Tahghighi, W. van Dijk, Christiaan Scott, Masaki Shimizu, Alexey Maletin, Braydon Meyer, Joost F Swart, Sylvia Costa Lima Farhat, Anna Taparkou, R. Fritsch-Stork, Paolo Cressoni, Reiji Hirano, I. Chyzheuskaya, Stefania Simou, Kseniya Isayeva, Mariluz Gámir Gámir, Paivi Miettunen, Francesca Ricci, Ruta Šantere, George Lazaros, Madeleine Rooney, Stefan Stefanov, Huseyin Ozkan, Céline La, Boris Hügle, Vita Dolžan, P. Barone, R. Gallizzi, Aline L. de Oliveira, Silvia Federici, Lauren J. Lahey, Kimme L. Hyrich, Claudia Saad-Magalhães, Selçuk Yüksel, Valda Stanevica, Silvia De Pauli, Seid-Reza Raeeskarami, Calin Lazar, Sema Akman, Laurence Chatel, Kirsten Minden, Ismaiel A. Tekko, Philipp Henneke, E. Cortis, Elena Košková, Gil Amarilyo, Ana M. Marín Sánchez, Antonella Insalaco, Z. Birsin Ozcakar, Melissa Mariti Fraga, Lena Klevenvall, Luis Lira, Phoi-Ngoc Duong, Tatiana Bzarova, Neus Quilis, Wilco de Jager, Gary Sterba, Rina Denisova, Miroslav Harjacek, Eve M D Smith, N Ruperto, Gemma Lepri, Evgeniya Chistyakova, Rachel Kaufmann, Liliana Lourenço Jorge, Violeta Panaviene, Helena Canhão, Riccardo Belli, Grigoris Pardalos, Larisa I. Zajtseva, Nicolino Ruperto, Ezgi Batu, Paola Montesano, Alexander Solyom, Nicola Smith, Ales Janda, Sagar Bhattad, Liora Harel, Philip N. Hawkins, Gozde Yucel, François Willermain, Paolo Picco, Alessandro Rimini, Gordana Susic, Esi M. Morgan, Jessica Beckmann, Arina Lazareva, Agustin Remesal, Özge Altuğ Gücenmez, Troels Herlin, Andreas Groll, T. Yuraga, Ekaterina Zaharova, Adriana E. M. Sallum, Zeynep Birsin Özçakar, D. Milojevic, Can Kosukcu, Isabella Ceccherini, Sandrine Lacassagne, Tania M. Castro, R. Consolini, Klaus Müller, Dogan Simsek, Frank Rühle, Katia Kozu, Femke van Wijk, Yasin Sahin, Jonathan S. Hausmann, Gokalp Basbozkurt, M. Cattalini, Mª José Santos, Norman T. Ilowite, Adriana M. E. Sallum, Simona Rednic, Sirisucha Soponkanaporn, Giancarla Di Landro, Semanur Özdel, Timothy R. Radstake, Anastasia Wiener, Betül Sözeri, Estefania Quesada-Masachs, E. Zholobova, Joshua Newson, Ozgur Kasapcopur, Davide Montin, Terence Flood, Amir Mendelson, Manuela Pardeo, Flávia Heloísa dos Santos, Jamie Eaton, Vignesh Pandiarajan, Lyudmila Belyaeva, Edson Amaro Junior, Claudio Arnaldo Len, Tamás Constantin, Livia de Freitas Keppeke, Cristina Ferrari, Margarita Soloshenko, C. Rabinovich, David Popp, Jeremy Sokolove, Jaymi Taiani, Chiara Passarelli, de Min Cristina, José Costa, Stefanie Herresthal, Thomas Giner, Laure Caspers, Dilek Konukbay, Ulrich Salzer, Jorre S. Mertens, Marijan Frković, Yosef Uziel, Sabrina Chiesa, Luisa Bracci-Laudiero, Anders Fasth, Raul A. Chavez Valencia, Jordan T. Jones, Francesca Lancini, Alessandra Ferrari, Dana Nemcova, Mark Difrancesco, Ricardo Figueira, John Mitchell, Zohreh Nademi, E. Fedorov, Thomas Vogl, Carine Wouters, Mónica Eusébio, Hannah Leahey, Alessandra Pontillo, Marco Gattorno, Mandica Vidović, Lucas L. van den Hoogen, Mikhail Kostik, Giovanni Corsello, Gian Marco Moneta, Richard Mouy, Mariana Rodrigues, Veronica Medeghini, Gökçe Gür, Lucas Kich Grun, Stephan Ehl, Edi Paleka Bosak, Walter Ferlin, Hanna Lythgoe, Tsuyoshi Yamatou, Navdha R. Ramchurn, Carolina Furtado, Estefania Barral, Cecilia Lazea, Nikolay Tzaribachev, Vahid Ziaee, Fatemeh Hadipour, Alberto Sifuentes Giraldo, Kimberly Gilmour, Marite Rygg, Anna Valenti, María M Katsicas, Raju Khubchandani, Despoina Maritsi, Alessandra Tesser, R. M. Laxer, Clotilde Alizzi, Francisco Rivas-Larrauri, Aysen Tezcaner, Anne Dennos, Vasiliki Tzimouli, Vibeke Strand, Banu Acar, Fabio Candotti, Kseniia V. Danilko, Joachim Schultze, María Luz Gámir Gámir, Alessia Omenetti, Berit Flatø, Ruth Eraso, Bernard Lauwerys, Angela Pistorio, Andressa G. F. Alves, Gerd Ganser, Sara Signa, Ana Lopes, Emese Kiss, Charlene Foley, Sylvia Kamphuis, Maja Di Rocco, Kenan Barut, Ilaria Parissenti, Aida Koka, Nicholas Ng, Francis Corazza, Vinícius L. Braga, Laura E. Schanberg, Karin Beutel, Camila Hirotsu, Jonathan D Akikusa, Mihaela Sparchez, Karoline Ehlert, Jordi Anton, Adriana M. Sallum, Maria Cristina Castiglione, Surjit Singh, Julie Jones, Katya Temelkova, Tania S. Amin, Jasmin B Kuemmerle-Deschner, Samantha Bell, Sakda A.-O. Vallipakorn, Manuel Salgado, Filipa Ramos, Balahan Makay, Nadezhda Tsurikova, Gianmaria Viglizzo, Rosalba Ferraro, Sandra Hansmann, Nilgün Çakar, Ismail Dursun, Maria Stavrakidou, T. Bzarova, Sally Pino, Dhouib Amira, Salla Kangas, Antonella Meini, Dirk Foell, Dolunay Gürses, Dace Bērziņa, A. Speziale, Juan I. Arostegui Gorospe, Kelly L. Mieszkalski, Dawn M. Wahezi, S. Davì, Radoslav Srp, Daniel J. Kingsbury, Alexei A Grom, Falcini Fernanda, Peng Yin, Claire T. Deakin, Eva Hlavackova, Pavla Doležalová, Maria Mercedes Picarelli, Ezgi D. Batu, Alessandra Tricarico, Soamarat Vilaiyuk, Ivan Costa-Filho, A. Civino, Lukas Hackl, Pilar Gomez, Michael Hofer, M Manuela Costa, Zbigniew Zuber, Elena Ligostaeva, Carlos D. Rose, Jozef Hoza, Pranoot Tanpaiboon, Bonnie Vlahos, Sandra Garrote Corral, Martina Finetti, Giedre Grigelioniene, Susanne M. Benseler, X Wei, Pieter Van Dijkhuizen, Lee Nelson, Elettra Santori, David Martino, Anju Gupta, Nuray Aktay Ayaz, Noa Rabinowicz, Susan Shenoi, Rachel Chiaroni-Clarke, Claudia Bracaglia, Ruhan Düşünsel, M. Hofer, Rolando Cimaz, Juan I. Aróstegui, Ana Filipa Mourão, Ivonne Arroyo, Laura Damian, Marco F. C. D. Silva, D. J. Lovell, Marta Torcoletti, Clara Malagón, Luisa Klotz, Krisztina Sevcic, Douglas Veale, Belen Serrano Benavente, N. Groot, Polyxeni Pratsidou, Nicole Johnson, Karen Wynne, SR Rodionovskaya, Melania Saifridova, Kaara Tiewsoh, Ryan F. Donnelly, Fernanda Falcini, Valérie Badot, M. G. Alpigiani, L. Breda, Farida Abduragimova, Veronika Gjertsen Rypdal, Sophie Hambleton, E. Chalom, Anna Horne, Antonio Novelli, O. Kostareva, Panagiotis Tziavas, Yara Barrense-Dias, Cecilia Bava, Sarah Ringold, William H. Robinson, Sirirat Charuvanij, D. Kingsbury, Shuichi Ito, Luiz A. A. Pereira, Marcus Herbert Jones, S. I. Valieva, Flavio Sztajnbok, Florence Guilhot, Cristina de Min, Adriana Diaz-Maldonado, Simone A. Lotufo, Beril Talim, M. Heinrich, Paul Newland, and Laura Pagani
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030203 arthritis & rheumatology ,03 medical and health sciences ,medicine.medical_specialty ,0302 clinical medicine ,Rheumatology ,business.industry ,030220 oncology & carcinogenesis ,Family medicine ,Pediatrics, Perinatology and Child Health ,medicine ,Immunology and Allergy ,business ,Paediatric rheumatology - Published
- 2017
17. Immunosenescence Induced by Plasma from Individuals with Obesity Caused Cell Signaling Dysfunction and Inflammation
- Author
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Mariana Migliorini, Parisi, Lucas Kich, Grun, Patrícia, Lavandoski, Letícia Biscaino, Alves, Ivi Juliana, Bristot, Rita, Mattiello, Cláudio Corá, Mottin, Fábio, Klamt, Marcus Herbert, Jones, Alexandre Vontobel, Padoin, Fátima Costa Rodrigues, Guma, and Florencia María, Barbé-Tuana
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Adult ,Inflammation ,Male ,Serum ,Immunosenescence ,Macrophages ,Interleukin-1beta ,Interleukin-8 ,Apoptosis ,CD8-Positive T-Lymphocytes ,Culture Media ,Leukocytes, Mononuclear ,Humans ,Female ,Obesity ,Signal Transduction - Abstract
To evaluate the consequences of plasma from individuals with obesity on parameters associated with immunosenescence in unrelated healthy peripheral blood mononuclear cells (PBMC).Freshly isolated PBMC were incubated in media supplemented with 10% of plasma from individuals with obesity or control subjects for the first 4 hours of 24 to 120 hours of culture.Plasma from individuals with obesity modulated the phenotype of healthy PBMC, leading to a higher rate of apoptosis, lower amounts of phospho-γH2AX and -p53, and mitochondrial dysfunction. After 120 hours, there was a higher secretion of inflammatory cytokines IL-1β and IL-8. CD8These results demonstrate that chronic systemic inflammation observed in obesity induces dysfunctional features in PBMC that are consistent with premature immunosenescence.
- Published
- 2017
18. Effects of previous physical exercise to chronic stress on long-term aversive memory and oxidative stress in amygdala and hippocampus of rats
- Author
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Janaína Kolling, Tiago Marcon dos Santos, Carla Dalmaz, Cassiana Siebert, Lucas Kich Grun, Carolina Gessinger Bertó, Florencia María Barbé-Tuana, Helena Biasibetti, and Angela T. S. Wyse
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0301 basic medicine ,Male ,medicine.medical_specialty ,Memory, Long-Term ,Alpha (ethology) ,Hippocampus ,Physical exercise ,medicine.disease_cause ,Amygdala ,Thiobarbituric Acid Reactive Substances ,03 medical and health sciences ,0302 clinical medicine ,Superoxide Dismutase-1 ,Developmental Neuroscience ,Internal medicine ,Physical Conditioning, Animal ,medicine ,Animals ,Chronic stress ,Longitudinal Studies ,Na+/K+-ATPase ,Rats, Wistar ,Analysis of Variance ,Memory Disorders ,Catalase ,Rats ,Inhibition, Psychological ,Oxidative Stress ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Chronic Disease ,Physical therapy ,Analysis of variance ,Sodium-Potassium-Exchanging ATPase ,Psychology ,030217 neurology & neurosurgery ,Oxidative stress ,Stress, Psychological ,Developmental Biology - Abstract
Since stressful situations are considered risk factors for the development of depression and there are few studies evaluating prevention therapies for this disease, in the present study we evaluated the effect of previous physical exercise in animals subjected to chronic variable stress (CVS), an animal model of depression, on behavior tasks. We also investigated some parameters of oxidative stress and Na+, K+-ATPase activity, immunocontent and gene expression of alpha subunits in amygdala and hippocampus of rats. Young male rats were randomized into four study groups (control, exercised, stressed, exercised + stressed). The animals were subjected to controlled exercise treadmill for 20 min,three times a week, for two months prior to submission to the CVS (40 days). Results show that CVS impaired performance in inhibitory avoidance at 24 h and 7 days after training session. CVS induced oxidative stress, increasing reactive species, lipoperoxidation and protein damage, and decreasing the activity of antioxidant enzymes. The activity of Na+, K+-ATPase was decreased, but the immunocontents and gene expression of catalytic subunits were not altered. The previous physical exercise was able to improve performance in inhibitory avoidance at 24 h after training; additionally, exercise prevented oxidative damage, but was unable to reverse completely the changes observed on the enzymatic activities. Our findings suggest that physical exercise during the developmental period may protect against aversive memory impairment and brain oxidative damage caused by chronic stress exposure later in life.
- Published
- 2016
19. Effect of obesity on telomere length: Systematic review and meta-analysis
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Eduardo, Mundstock, Edgar E, Sarria, Helen, Zatti, Fernanda, Mattos Louzada, Lucas, Kich Grun, Marcus, Herbert Jones, Fátima T C R, Guma, João, Mazzola In Memoriam, Matias, Epifanio, Renato T, Stein, Florencia M, Barbé-Tuana, and Rita, Mattiello
- Subjects
Body Weight ,Leukocytes ,Humans ,Telomere Homeostasis ,Female ,Obesity ,Overweight ,Telomere ,Adiposity - Abstract
The main objective of this systematic review is to assess the effects of obesity on telomere length.The following databases were searched: MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials (CENTRAL, The Cochrane Library), LILACS, SPORTdiscus, and Web of Science from inception to August 2014. The search was performed using the following combinations of terms: telomere AND "overweight" OR "obesity" OR "adiposity," without language restriction.Sixty-three original studies were included in this systematic review, comprising 119,439 subjects. Thirty-nine studies showed either weak or moderate correlation between obesity and telomere length; however, they showed an important heterogeneity.There is a tendency toward demonstrating negative correlation between obesity and telomere length. The selected studies showed weak to moderate correlation for the main search, and there was an important heterogeneity. For this reason, the causal relationship of obesity and telomere length remains open. Additional controlled longitudinal studies are needed to investigate this issue.
- Published
- 2015
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