18 results on '"Luca D'Alessio"'
Search Results
2. A Hybrid Primitive-Based Navigation Planner for the Wheeled-Legged Robot CENTAURO
- Author
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De Luca, Alessio, Muratore, Luca, and Tsagarakis, Nikos G.
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Sensor-based Control, Motion and Path Plan- ning, Legged Robots - Abstract
Wheeled-legged robots have the potential to navigate in cluttered and irregular scenarios by altering the locomotion modes to adapt to the terrain challenges and effectively reach targeted locations in unstructured spaces. To achieve this functionality, a hybrid locomotion planner is necessary. In this work we present a search-based planner, which explores a set of motion primitives and a 2.5D traversability map extracted from the environment to generate navigation plans for the hybrid mobility robot CENTAURO. The planner explores the map from the current robot position to the goal location requested by the user, considering the most appropriate composition and tuning of locomotion primitives to build up afeasible plan, which is then executed by the robot. The available primitives are prioritized and can be easily modified, addedor removed through a configuration file. Our approach wasevaluated both in simulation and on the real wheeled-leggedrobot CENTAURO, demonstrating traversing capabilities incluttered environments with various obstacles.
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- 2022
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3. Non-Isothermal Reduction Kinetics of Iron Ore-Based Oxygen Carrier Materials for Biomass Chemical-Looping Gasification
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Chichi (Xueqi) Zhang, Alex Chi-Kin Yip, Luca Alessio, and Shusheng Pang
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History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
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4. Adiabatic perturbation theory and geometry of periodically-driven systems
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Luca D'Alessio, Anatoli Polkovnikov, Phillip Weinberg, Szabolcs Vajna, Michael Kolodrubetz, and Marin Bukov
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Floquet theory ,Photon ,FOS: Physical sciences ,General Physics and Astronomy ,01 natural sciences ,010305 fluids & plasmas ,Adiabatic theorem ,Condensed Matter - Strongly Correlated Electrons ,symbols.namesake ,0103 physical sciences ,010306 general physics ,Adiabatic process ,Physics ,Quantum Physics ,Chern class ,Strongly Correlated Electrons (cond-mat.str-el) ,Observable ,Condensed Matter - Other Condensed Matter ,Quantum Gases (cond-mat.quant-gas) ,Quantum electrodynamics ,symbols ,Berry connection and curvature ,Quantum Physics (quant-ph) ,Condensed Matter - Quantum Gases ,Hamiltonian (quantum mechanics) ,Other Condensed Matter (cond-mat.other) - Abstract
We give a systematic review of the adiabatic theorem and the leading non-adiabatic corrections in periodically-driven (Floquet) systems. These corrections have a two-fold origin: (i) conventional ones originating from the gradually changing Floquet Hamiltonian and (ii) corrections originating from changing the micro-motion operator. These corrections conspire to give a Hall-type linear response for non-stroboscopic (time-averaged) observables allowing one to measure the Berry curvature and the Chern number related to the Floquet Hamiltonian, thus extending these concepts to periodically-driven many-body systems. The non-zero Floquet Chern number allows one to realize a Thouless energy pump, where one can adiabatically add energy to the system in discrete units of the driving frequency. We discuss the validity of Floquet Adiabatic Perturbation Theory (FAPT) using five different models covering linear and non-linear few and many-particle systems. We argue that in interacting systems, even in the stable high-frequency regimes, FAPT breaks down at ultra slow ramp rates due to avoided crossings of photon resonances, not captured by the inverse-frequency expansion, leading to a counter-intuitive stronger heating at slower ramp rates. Nevertheless, large windows in the ramp rate are shown to exist for which the physics of interacting driven systems is well captured by FAPT.
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- 2017
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5. Vehicle aerodynamics impact of on-road turbulence
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Joaquin Gargoloff, Luca D'Alessio, Bradley Duncan, and Ales Alajbegovic
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0209 industrial biotechnology ,Engineering ,business.industry ,Turbulence ,Mechanical Engineering ,Aerospace Engineering ,020302 automobile design & engineering ,02 engineering and technology ,Aerodynamics ,Aerodynamic force ,020901 industrial engineering & automation ,0203 mechanical engineering ,Automobile handling ,Aerospace engineering ,business ,Crosswind - Abstract
The ultimate target for vehicle aerodynamicists is to develop vehicles that perform well on the road in real-world conditions. On the other hand, vehicle development today is performed mostly in controlled settings, using wind tunnels and computational fluid dynamics with artificially uniform freestream conditions and neglecting real-world effects due to road turbulence from the wind and other vehicles. Turbulence on the road creates a non-uniform and fluctuating flow field in which the length scales of the fluctuations fully encompass the length scales of the relevant aerodynamic flow structures around the vehicle. These fluctuations can be comparable in size and strength with the vehicle’s own wake oscillations. As a result, this flow environment can have a significant impact on the aerodynamic forces and on the sensitivity of these forces to various shape changes. Some aerodynamic devices and integral design features can perform quite differently from the way in which they do under uniform freestream conditions. In this paper, unsteady aerodynamics simulations are performed using the lattice Boltzmann method on a detailed representative automobile model with several design variants, in order to explore the effect of on-road turbulence on the aerodynamics and the various mechanisms that contribute to these effects.
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- 2017
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6. Accurate Fuel Economy Prediction via a Realistic Wind Averaged Drag Coefficient
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Joaquin Gargoloff, Luca D'Alessio, Bradley Duncan, Chin-Wei Chang, and Edward D. Tate
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050210 logistics & transportation ,Drag coefficient ,Engineering ,business.industry ,05 social sciences ,02 engineering and technology ,General Medicine ,Aerodynamics ,020303 mechanical engineering & transports ,0203 mechanical engineering ,Drag ,Automobile drag coefficient ,0502 economics and business ,Fuel efficiency ,Aerospace engineering ,business ,Automotive aerodynamics - Published
- 2017
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7. From quantum chaos and eigenstate thermalization to statistical mechanics and thermodynamics
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Yariv Kafri, Marcos Rigol, Luca D'Alessio, and Anatoli Polkovnikov
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media_common.quotation_subject ,FOS: Physical sciences ,Thermodynamics ,Second law of thermodynamics ,01 natural sciences ,010305 fluids & plasmas ,Condensed Matter - Strongly Correlated Electrons ,0103 physical sciences ,010306 general physics ,Quantum ,Eigenstate thermalization hypothesis ,Condensed Matter - Statistical Mechanics ,media_common ,Physics ,Quantum Physics ,Statistical Mechanics (cond-mat.stat-mech) ,Strongly Correlated Electrons (cond-mat.str-el) ,Fundamental thermodynamic relation ,Observable ,Statistical mechanics ,Condensed Matter Physics ,Quantum chaos ,Quantum Gases (cond-mat.quant-gas) ,Condensed Matter - Quantum Gases ,Quantum Physics (quant-ph) ,Random matrix - Abstract
This review gives a pedagogical introduction to the eigenstate thermalization hypothesis (ETH), its basis, and its implications to statistical mechanics and thermodynamics. In the first part, ETH is introduced as a natural extension of ideas from quantum chaos and random matrix theory (RMT). To this end, we present a brief overview of classical and quantum chaos, as well as RMT and some of its most important predictions. The latter include the statistics of energy levels, eigenstate components, and matrix elements of observables. Building on these, we introduce the ETH and show that it allows one to describe thermalization in isolated chaotic systems without invoking the notion of an external bath. We examine numerical evidence of eigenstate thermalization from studies of many-body lattice systems. We also introduce the concept of a quench as a means of taking isolated systems out of equilibrium, and discuss results of numerical experiments on quantum quenches. The second part of the review explores the implications of quantum chaos and ETH to thermodynamics. Basic thermodynamic relations are derived, including the second law of thermodynamics, the fundamental thermodynamic relation, fluctuation theorems, and the Einstein and Onsager relations. In particular, it is shown that quantum chaos allows one to prove these relations for individual Hamiltonian eigenstates and thus extend them to arbitrary stationary statistical ensembles. We then show how one can use these relations to obtain nontrivial universal energy distributions in continuously driven systems. At the end of the review, we briefly discuss the relaxation dynamics and description after relaxation of integrable quantum systems, for which ETH is violated. We introduce the concept of the generalized Gibbs ensemble, and discuss its connection with ideas of prethermalization in weakly interacting systems., Comment: 130 pages, 36 figures, as published
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- 2016
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8. Universal high-frequency behavior of periodically driven systems: from dynamical stabilization to Floquet engineering
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Anatoli Polkovnikov, Luca D'Alessio, and Marin Bukov
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Condensed Matter::Quantum Gases ,Physics ,Density matrix ,Floquet theory ,Statistical Mechanics (cond-mat.stat-mech) ,FOS: Physical sciences ,Observable ,Condensed Matter Physics ,01 natural sciences ,010305 fluids & plasmas ,Magnetic field ,symbols.namesake ,Classical mechanics ,Quantum Gases (cond-mat.quant-gas) ,0103 physical sciences ,symbols ,Condensed Matter - Quantum Gases ,010306 general physics ,Hamiltonian (quantum mechanics) ,Condensed Matter - Statistical Mechanics - Abstract
We give a general overview of the high-frequency regime in periodically driven systems and identify three distinct classes of driving protocols in which the infinite-frequency Floquet Hamiltonian is not equal to the time-averaged Hamiltonian. These classes cover systems, such as the Kapitza pendulum, the Harper-Hofstadter model of neutral atoms in a magnetic field, the Haldane Floquet Chern insulator and others. In all setups considered, we discuss both the infinite-frequency limit and the leading finite-frequency corrections to the Floquet Hamiltonian. We provide a short overview of Floquet theory focusing on the gauge structure associated with the choice of stroboscopic frame and the differences between stroboscopic and non-stroboscopic dynamics. In the latter case one has to work with dressed operators representing observables and a dressed density matrix. We also comment on the application of Floquet Theory to systems described by static Hamiltonians with well-separated energy scales and, in particular, discuss parallels between the inverse-frequency expansion and the Schrieffer-Wolff transformation extending the latter to driven systems., 84 pages, 25 figures, 4 appendices
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- 2015
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9. Many-body energy localization transition in periodically driven systems
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Luca D'Alessio and Anatoli Polkovnikov
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Physics ,Quantum Physics ,Statistical Mechanics (cond-mat.stat-mech) ,media_common.quotation_subject ,Complex system ,FOS: Physical sciences ,General Physics and Astronomy ,Fermi acceleration ,Second law of thermodynamics ,01 natural sciences ,Charged particle ,010305 fluids & plasmas ,Classical mechanics ,13. Climate action ,0103 physical sciences ,Thermodynamic limit ,Ergodic theory ,Quantum Physics (quant-ph) ,010306 general physics ,Entropy (arrow of time) ,Quantum ,Condensed Matter - Statistical Mechanics ,media_common - Abstract
According to the second law of thermodynamics the total entropy of a system is increased during almost any dynamical process. The positivity of the specific heat implies that the entropy increase is associated with heating. This is generally true both at the single particle level, like in the Fermi acceleration mechanism of charged particles reflected by magnetic mirrors, and for complex systems in everyday devices. Notable exceptions are known in noninteracting systems of particles moving in periodic potentials. Here the phenomenon of dynamical localization can prevent heating beyond certain threshold. The dynamical localization is known to occur both at classical (Fermi-Ulam model) and at quantum levels (kicked rotor). However, it was believed that driven ergodic systems will always heat without bound. Here, on the contrary, we report strong evidence of dynamical localization transition in periodically driven ergodic systems in the thermodynamic limit. This phenomenon is reminiscent of many-body localization in energy space., Comment: final (printed) version
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- 2013
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10. First principles study of the vibrational properties of
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Marco Bernasconi, Luca D'Alessio, and F. Pietrucci
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Chemistry ,Analytical chemistry ,Experimental data ,Infrared spectroscopy ,General Chemistry ,Condensed Matter Physics ,Molecular physics ,symbols.namesake ,Ab initio quantum chemistry methods ,Molecular vibration ,symbols ,General Materials Science ,Perturbation theory ,Raman spectroscopy - Abstract
Based on density functional perturbation theory, we have studied the vibrational properties of crystalline Li 2 TeO 3 . The calculated Raman and IR spectra are in good agreement with available experimental data and allow assigning the experimental peaks to specific phononic modes.
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- 2007
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11. Emergent Newtonian dynamics and the geometric origin of mass
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Luca D'Alessio and Anatoli Polkovnikov
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Physics ,Quantum Physics ,Statistical Mechanics (cond-mat.stat-mech) ,Hilbert space ,Degrees of freedom (statistics) ,General Physics and Astronomy ,FOS: Physical sciences ,01 natural sciences ,Classical limit ,010305 fluids & plasmas ,Newtonian dynamics ,Hamiltonian system ,symbols.namesake ,Classical mechanics ,0103 physical sciences ,symbols ,Dissipative system ,Berry connection and curvature ,10. No inequality ,010306 general physics ,Hamiltonian (quantum mechanics) ,Quantum Physics (quant-ph) ,Condensed Matter - Statistical Mechanics - Abstract
We consider a set of macroscopic (classical) degrees of freedom coupled to an arbitrary many-particle Hamiltonian system, quantum or classical. These degrees of freedom can represent positions of objects in space, their angles, shape distortions, magnetization, currents and so on. Expanding their dynamics near the adiabatic limit we find the emergent Newton's second law (force is equal to the mass times acceleration) with an extra dissipative term. In systems with broken time reversal symmetry there is an additional Coriolis type force proportional to the Berry curvature. We give the microscopic definition of the mass tensor relating it to the non-equal time correlation functions in equilibrium or alternatively expressing it through dressing by virtual excitations in the system. In the classical (high-temperature) limit the mass tensor is given by the product of the inverse temperature and the Fubini-Study metric tensor determining the natural distance between the eigenstates of the Hamiltonian. For free particles this result reduces to the conventional definition of mass. This finding shows that any mass, at least in the classical limit, emerges from the distortions of the Hilbert space highlighting deep connections between any motion (not necessarily in space) and geometry. We illustrate our findings with four simple examples., Comment: 28 + 14 pages, 4 figures
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- 2013
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12. Topological Versus Physical and Chemical Properties of Negatively Curved Carbon Surfaces
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Mihai V. Putz, Marzio De Corato, Giorgio Benedek, Luca D'Alessio, Marco Bernasconi, and Ottorino Ori
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symbols.namesake ,Materials science ,Ab initio quantum chemistry methods ,Structural stability ,Topological index ,Ab initio ,Gaussian curvature ,symbols ,Wiener index ,Hyperboloid ,Topology ,Topology (chemistry) - Abstract
Some relevant physical and chemical properties of negatively curved carbon surfaces like sp 2-bonded schwarzites can be predicted or accounted for on the basis of purely topological arguments. The general features of the vibrational spectrum of complex sp 2-carbon structures depend primarily on the topology of the bond network and can be estimated, in a first approximation and for systems with only nearest-neighbor interactions, from the diagonalization of the adjacency matrix. Examples are discussed for three- and two-periodic carbon schwarzites, where a direct comparison with ab initio calculations is possible. The spectral modifications produced by the insertion of defects can also analyzed on pure topological grounds. Two-periodic (planar) schwarzites can be viewed as regular arrays of Y-shaped nanojunctions, which are basic ingredients of carbon-based nano-circuits. A special class of planar schwarzites is obtained from a modification of a graphene bilayer where the two sheets are linked by a periodic array of hyperboloid necks with a negative Gaussian curvature. Ab initio density functional calculations for some structures among the simplest planar schwarzites – (C18)2, (C26)2, and (C38)2 – are presented and discussed in light of the structural stability predictions derived from a topological graph-theory analysis based on the Wiener index. A quantum-mechanical justification is provided for the effectiveness of the Wiener index in ranking the structural stability of different sp 2-conjugated structures.
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- 2013
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13. Negative mass corrections in a dissipative stochastic environment
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Yariv Kafri, Luca D'Alessio, and Anatoli Polkovnikov
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Statistics and Probability ,Physics ,Oscillation ,Statistical and Nonlinear Physics ,01 natural sciences ,010305 fluids & plasmas ,Classical mechanics ,Negative mass ,0103 physical sciences ,Dissipative system ,Statistics, Probability and Uncertainty ,Perturbation theory ,010306 general physics ,Reduction (mathematics) ,Adiabatic process ,Harmonic oscillator ,Spin-½ - Abstract
We study the dynamics of a macroscopic object interacting with a dissipative stochastic environment using an adiabatic perturbation theory. The perturbation theory reproduces known expressions for the friction coefficient and, surprisingly, gives an additional negative mass correction. The effect of the negative mass correction is illustrated by studying a harmonic oscillator interacting with a dissipative stochastic environment. While it is well known that the friction coefficient causes a reduction of the oscillation frequency, we show that the negative mass correction can lead to its enhancement. By studying an exactly solvable model of a magnet coupled to a spin environment evolving under standard non-conserving dynamics we show that the effect is present even beyond the validity of the adiabatic perturbation theory.
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- 2016
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14. Universal energy fluctuations in thermally isolated driven systems
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Guy Bunin, Anatoli Polkovnikov, Luca D'Alessio, and Yariv Kafri
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Physics ,Work (thermodynamics) ,Energy distribution ,Heat bath ,Statistical Mechanics (cond-mat.stat-mech) ,Final energy ,General Physics and Astronomy ,FOS: Physical sciences ,01 natural sciences ,Boltzmann distribution ,010305 fluids & plasmas ,Gibbs free energy ,Isolated system ,symbols.namesake ,Quantum Gases (cond-mat.quant-gas) ,0103 physical sciences ,symbols ,Statistical physics ,010306 general physics ,Condensed Matter - Quantum Gases ,Energy (signal processing) ,Condensed Matter - Statistical Mechanics - Abstract
When an isolated system is brought in contact with a heat bath its final energy is random and follows the Gibbs distribution -- a cornerstone of statistical physics. The system's energy can also be changed by performing non-adiabatic work using a cyclic process. Almost nothing is known about the resulting energy distribution in this setup, which is especially relevant to recent experimental progress in cold atoms, ions traps, superconducting qubits and other systems. Here we show that when the non-adiabatic process comprises of many repeated cyclic processes the resulting energy distribution is universal and different from the Gibbs ensemble. We predict the existence of two qualitatively different regimes with a continuous second order like transition between them. We illustrate our approach performing explicit calculations for both interacting and non-interacting systems.
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- 2011
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15. Light impurity in an equilibrium gas
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P. L. Krapivsky and Luca D'Alessio
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Thermal equilibrium ,Physics ,Statistical Mechanics (cond-mat.stat-mech) ,Sublinear function ,Lorentz transformation ,Dimension (graph theory) ,FOS: Physical sciences ,Particle displacement ,Lambda ,01 natural sciences ,010305 fluids & plasmas ,symbols.namesake ,Quantum mechanics ,0103 physical sciences ,symbols ,Exponent ,Atomic physics ,010306 general physics ,Scaling ,Condensed Matter - Statistical Mechanics - Abstract
We investigate the evolution of a light impurity particle in a Lorentz gas where the background atoms are in thermal equilibrium. As in the standard Lorentz gas, we assume that the particle is negligibly light in comparison with the background atoms. The thermal motion of atoms causes the average particle speed to grow. In the case of the hard-sphere particle-atom interaction, the temporal growth is ballistic, while generally it is sub-linear. For the particle-atom potential that diverges as r^{-\lambda} in the small separation limit, the average particle speed grows as t^{\lambda /(2(d-1)+ \lambda)} in d dimensions. The particle displacement exhibits a universal growth, linear in time and the average (thermal) speed of the atoms. Surprisingly, the asymptotic growth is independent on the gas density and the particle-atom interaction. The velocity and position distributions approach universal scaling forms which are non-Gaussian. We determine the velocity distribution in arbitrary dimension and for arbitrary interaction exponent \lambda. For the hard-sphere particle-atom interaction, we compute the position distribution and the joint velocity-position distribution., Comment: version 2, 22 pages, 9 figures
- Published
- 2010
16. Left Ventricle Performance by Longitudinal Peak Systolic Strain Measurement In Young Athletes With Bicuspid Aortic Valve
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Giorgio Galanti, Loira Toncelli, De Luca Alessio, Luigi Caselli, Brunello Cappelli, Laura Stefani, Gabriele Innocenti, and Roberto Mercuri
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medicine.medical_specialty ,biology ,Athletes ,business.industry ,Physical Therapy, Sports Therapy and Rehabilitation ,Peak systolic strain ,medicine.disease ,biology.organism_classification ,medicine.anatomical_structure ,Bicuspid aortic valve ,Afterload ,Ventricle ,Internal medicine ,medicine ,Cardiology ,Orthopedics and Sports Medicine ,business - Published
- 2009
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17. SEIFEM 2010-E: economic evaluation of posaconazole for antifungal prophylaxis in patients with acute myeloid leukemia receiving induction chemotherapy
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Chiara Cattaneo, Federica Lessi, Giordana Martino, Mario Delia, Rosa Fanci, Franco Aversa, Mario Tumbarello, Annamaria Nosari, Alessio De Luca, Livio Pagano, Simone Cesaro, Giulia Dragonetti, Anna Candoni, Alessandro Busca, Gaspare Guglielmi, Luisa Verga, Marco Picardi, Leonardo Potenza, Gianpaolo Nadali, Busca, Alessandro, Lessi, Federica, Verga, Luisa, Candoni, Anna, Cattaneo, Chiara, Cesaro, Simone, Dragonetti, Giulia, Delia, Mario, De Luca, Alessio, Guglielmi, Gaspare, Tumbarello, Mario, Martino, Giordana, Nadali, Gianpaolo, Fanci, Rosa, Picardi, Marco, Potenza, Leonardo, Nosari, Annamaria, Aversa, Franco, and Pagano, Livio
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Male ,acute myeloid leukemia ,Antifungal prophylaxis ,antifungal treatment ,cost ,Adult ,Aged ,Aged, 80 and over ,Antifungal Agents ,Antineoplastic Combined Chemotherapy Protocols ,Cost-Benefit Analysis ,Drug Costs ,Female ,Humans ,Induction Chemotherapy ,Leukemia, Myeloid, Acute ,Middle Aged ,Monte Carlo Method ,Mycoses ,Outcome Assessment (Health Care) ,Triazoles ,Young Adult ,Antibiotic Prophylaxis ,Hematology ,Oncology ,Cancer Research ,Posaconazole ,Mycose ,Pharmacology ,0302 clinical medicine ,Outcome Assessment, Health Care ,Antifungal Agent ,030212 general & internal medicine ,Antibiotic prophylaxis ,Young adult ,Drug Cost ,Myeloid leukemia ,030220 oncology & carcinogenesis ,Human ,medicine.drug ,medicine.medical_specialty ,Itraconazole ,03 medical and health sciences ,Internal medicine ,medicine ,Antibiotic Prophylaxi ,Antifungal prophylaxi ,Cost-Benefit Analysi ,Antineoplastic Combined Chemotherapy Protocol ,business.industry ,Induction chemotherapy ,Settore MED/15 - MALATTIE DEL SANGUE ,Triazole ,business ,Fluconazole - Abstract
Posaconazole demonstrated clinical superiority over fluconazole and itraconazole for prophylaxis of mold infections, although concerns exist regarding the high acquisition cost for posaconazole. In this respect, we sought to analyze the costs of antifungal prophylaxis in patients with acute myeloid leukemia (AML) who received prophylactic posaconazole (n = 510, 58%), itraconazole (n = 120, 14%) or fluconazole (n = 175, 20%) during induction chemotherapy. The estimated cost of antifungal prophylaxis as well as the costs of subsequent systemic antifungal therapy for treatening an invasive fungal infections (IFI) was higher in the posaconazole group compared to itraconazole and fluconazole groups. Based on the Monte Carlo simulations, the itraconazole group had the highest cost, followed by the posaconazole and fluconazole group, although the overall survival was higher in the posaconazole group as compared to the other groups. In conclusion, the cost of prophylaxis with posaconazole in AML patients compares favorably with conventional antifungal agents.
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- 2017
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18. A comparative analysis of label-free liquid chromatography-mass spectrometry liver proteomic profiles highlights metabolic differences between pig breeds
- Author
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Giuliano Galimberti, Stefania Dall'Olio, Alessio Di Luca, Luca Fontanesi, Samuele Bovo, Bovo, Samuele, Luca, Alessio Di, Galimberti, Giuliano, Dall’Olio, Stefania, and Fontanesi, Luca
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0301 basic medicine ,Proteomics ,Swine ,In silico ,Protein metabolism ,lcsh:Medicine ,Quantitative trait locus ,Biology ,Breeding ,Feed conversion ratio ,Mass Spectrometry ,03 medical and health sciences ,chemistry.chemical_compound ,Species Specificity ,Liquid chromatography–mass spectrometry ,Animals ,Carboxylesterase 3 ,lcsh:Science ,Gene ,030304 developmental biology ,2. Zero hunger ,0303 health sciences ,Multidisciplinary ,Fatty acid metabolism ,lcsh:R ,030302 biochemistry & molecular biology ,Proteomic ,Metabolism ,Duroc ,Fatty acid synthase ,030104 developmental biology ,chemistry ,Biochemistry ,Gene Expression Regulation ,Liver ,Proteome ,Large White ,biology.protein ,lcsh:Q ,Label-free LC-MS ,Chromatography, Liquid - Abstract
The liver is a complex organ governing several physiological processes that define biological mechanisms affecting growth, feed efficiency and performance traits in all livestock species, including pig. Proteomics may contribute to a better understanding of the relationship between liver functions and complex production traits in pigs and to characterize this species as biomedical model. This study applied, for the first time, a label-free liquid chromatography-mass spectrometry (LC-MS) proteomic approach to compare the liver proteome profiles of two important heavy pig breeds, Italian Duroc and Italian Large White. Liver specimens were collected (after slaughtering) from performance tested pigs of these two breeds, raised in standard conditions. The label-free LC-MS method captured a total of 501 proteins of which 200 were subsequently considered in the between breeds comparison. A statistical pipeline based on the sparse Partial Least Squares Discriminant Analysis (sPLS-DA), coupled with stability and significance tests, was applied for the identification of up or down regulated proteins between breeds. This analysis revealed a total of 25 proteins clearly separating Italian Duroc and Italian Large White pigs. Among the top proteins differentiating the two breeds, 3-ketoacyl-CoA thiolase, mitochondrial (ACAA2) and histone H2B type 2-F (HIST2H2BF) were up-regulated in Italian Duroc pigs and carboxylesterase 3 (CES3) and ketohexokinase (KHK) were up-regulated in Italian Large White pigs. Fatty acid synthase (FASN), involved in fatty acid metabolism and encoded by a gene located in a QTL region for fatty acid composition, was up-regulated in Italian Large White pigs. The in silico protein interaction analysis showed that 16 of these proteins were connected in one big module. Bioinformatic functional analysis indicated that differentially expressed proteins were involved in several biological processes related to the metabolism of lipids, amino-acids, carbohydrates, cofactors and antibiotics/drugs, suggesting that these functions might distinguish Italian Duroc and Italian Large White pigs. This pilot comparative proteomic analysis of the porcine liver highlighted several biological factors that could determine the peculiar production potentials of these two heavy pig breeds, derived by their different genetic backgrounds.
- Published
- 2018
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