102 results on '"Lorena Gutiérrez"'
Search Results
2. Exploring the relationship between midwives’ work environment, women's safety culture, and intent to stay
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Mª Carmen Rodríguez-García, Isabel M. Martos-López, Gema Casas-López, Verónica V. Márquez-Hernández, Gabriel Aguilera-Manrique, and Lorena Gutiérrez-Puertas
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Maternity and Midwifery ,Obstetrics and Gynecology - Abstract
The shortage of midwives is a concern for healthcare systems as it compromises the quality maternity care. Various studies argue that a favorable work environment increases nurses' job satisfaction and intention to continue working at their current workplace.To analyze the work environment and its relationship with women's clinical safety culture and midwives' intention to stay in their current job and the midwifery profession.A cross-sectional, correlational study was performed on N = 218 midwives working in Spain. Standardized instruments were used, including The Practice Environment Scale of the Nursing Work Index (PES-NWI) and the Hospital Survey on Patient Safety Culture (HSOPSC). Descriptive and bivariable statistics were used. The study followed the STROBE guidelines.The work environment in the labor wards was mixed, according to the PES-NWI classification. The mean total score of the PES-NWI significantly and positively correlated with the mean total score of the HSOPSC (rs = 0.498, p 0.001), indicating that as the quality of midwives' work environment increased, women's clinical safety increased. Significant correlations were observed between the midwives' intent to stay in the hospital where they work and features of women's safety culture.The results of this study showed significant relationships between the work environment, women's safety culture, and midwives' intentions to leave their job/profession. Creating a favorable working environment could be a potentially effective strategy that encourages improvement in the women's safety culture in healthcare organizations and greater intention of midwives to stay at their current job.
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- 2023
3. Differential virome composition and richness between children's diarrheagenic stools kept at ultra-low temperatures for long-term
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Adriana Becerra, Pavel Iša, Ana Lorena Gutiérrez-Escolano, Federico Raúl Velázquez, Javier Torres, Carlos Federico Arias, and Teresa Estrada-Garcia
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Infectious Diseases ,Virology ,Parasitology ,General Medicine ,Microbiology - Abstract
Introduction: Diarrhoeal illness is the second cause of morbidity/mortality among children from less-developed regions worldwide. Nonetheless, there is scarce information regarding their gut microbiome. Aim: Microbiome characterization, with an emphasis on the virome, of children’s stools with diarrhoea, by a commercial microbiome array. Methodology: Nucleic acids extraction, optimised for viral identification, of stool samples from 20 Mexican children with diarrhoea (10 children < 2 and 10 ≥ 2-years-old), collected 16 years ago and kept at -70 °C, were analysed for the presence of viruses, bacteria, archaea, protozoa, and fungi species sequences. Results: Only viral and bacterial species sequences were identified among children’s stools. Most stool samples harboured species belonging to the bacteriophages (95%), anellovirus (60%), diarrhoeagenic viruses (40%), and non-human pathogens viruses (45% avian virus and 40% plant viruses) groups. Among the children's stools, virome inter-individual species composition was observed, even in presence of illness. The < 2-years-old children group has significantly higher viral richness (p = 0.01), conferred mainly by bacteriophages and diarrheagenic-viruses (p = 0.01) species, in comparison with the ≥ 2-years-old group. Conclusions: The virome of stools of children with diarrhoea revealed inter-individual viral species composition. Similarly, to the few virome studies in healthy young children, the bacteriophages group was the most abundant. A significantly higher viral richness, conferred by bacteriophages and diarrheagenic-viral species, was observed among < 2-years-old children in comparison with older children. Stools preserved at -70 °C for long term can successfully be used for microbiome studies.
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- 2023
4. Las compensaciones y el salario emocional en el sector comercio de Villavicencio – Colombia
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Stephani Lorena Gutiérrez Rincón and Dagoberto Torres Flórez
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Computer Networks and Communications ,Hardware and Architecture ,Software - Abstract
Las compensaciones y el salario emocional en las empresas del sector comercio en Villavicencio-Meta, Colombia, han sido poco influidas por situaciones y dinámicas como la rotación del personal y el limitado crecimiento de las personas por tener estructuras organizacionales ampliadas, siendo las remuneraciones afectadas a razón de la migración hacia otros sectores de la economía, se busca analizar las compensaciones y el salario emocional con el fin de brindar elementos que mejoren las prácticas de gestión humana. Se realizó una investigación de tipo cuantitativa con enfoque inferencial, con un muestreo no probabilístico, a partir de la base de datos recolectada en el año 2020, por parte del grupo de investigación GYDO con el instrumento Mp05c, sección compensaciones, el cual se aplicó de forma virtual a los colaboradores de las empresas de la ciudad; se encontró que existe una correlación negativa entre el promedio salarial y el salario emocional, además que se tienden a contar con una frecuencia de aplicación de beneficios sociales extralegales muy baja, y se concluye que el sector les ofrece a los colaboradores estabilidad laboral y salarial; sin embargo, los salarios no son significativos en comparación con otros sectores, esto debido a que la mayoría de empresas que conforman el sector son micro y pequeñas empresas.
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- 2023
5. What is essential remains invisible to the eyes? Blood pressure cuffs colonized by bacterial diversity
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José Miguel Garrido-Molina, Verónica V. Márquez-Hernández, Alba García-Viola, Manuel Ángel Rodríguez-Maresca, Javier García del Águila, and Lorena Gutiérrez-Puertas
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Microbiology (medical) ,Microbiology - Abstract
Using sphygmomanometers to measure blood pressure is a common practice in the healthcare context. The disinfection and maintenance of these devices is essential in clinical practice to prevent the proliferation of microorganisms. The aim of this study was to determine the presence of pathogenic microorganisms in sphygmomanometer cuffs in the clinical setting. A cross-sectional study was carried out. Five types of healthcare centers, selected through convenience sampling, participated in this study. Samples were collected from the inside of sphygmomanometer cuffs, and labeled and delivered to the laboratory for analysis. The samples were incubated in an oven at 35.5 °C for 24 h. A total CFU count was carried out on the plates that were cataloged as positive. Colonies that showed growth were identified using the matrix-assisted laser desorption/ionization-mass spectrometry technology. Of the total sample, (N = 372), 69.1% were positive and were isolated. In 30.9% (n = 115), no bacterial development was found within 48 h. A total of 257 microorganisms were found. The mean number of colony-forming units was 29.62 (SD = 32.33). The socio-health centers had the highest amount of bacterial contamination in the cuffs. In regards to the type of microorganisms, 31.5% (n = 81) found were Bacillus cereus, followed by 26.8% (n = 69) of Staphylococcus hominis and 9.7% (n = 25) were Pantoea agglomerans, among others. Statistically significant differences were found between the type of microorganism and the hours elapsed since the last disinfection (X
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- 2022
6. Hb Nivaria: A New Hemoglobin Variant with a Shortened α-Globin Chain [α139(HC1)Lys→Stop; HBA1: c.418A>T]
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Paloma Ropero Gradilla, José María Raya, Fernando Ataúlfo González, Sara Rochas, Sara Ferrer-Benito, Jorge M. Nieto, Taida Martín-Santos, Marcelo Barrios, Lorena Gutiérrez-Murillo, Ana Villegas, and Celina Benavente
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Biochemistry (medical) ,Clinical Biochemistry ,Hematology ,Genetics (clinical) - Published
- 2022
7. Educación in Our Own Terms: Survivance Amongst Latinx Migrant and Seasonal Farmworkers in K-12 Schools and Beyond
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Lorena Gutiérrez
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Cultural Studies ,Education - Published
- 2022
8. Self-Efficacy in the Cannulation Technique for Intraosseous Access in Pediatric Cardiac Arrest
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Verónica V. Márquez-Hernández, Lorena Gutiérrez-Puertas, Alba García-Viola, José Miguel Garrido-Molina, Vanesa Gutiérrez-Puertas, Gabriel Aguilera-Manrique, and Ma. Carmen Rodríguez-García
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Pediatrics, Perinatology and Child Health ,Emergency Medicine ,General Medicine - Published
- 2023
9. Nivel de autopercepción en mujeres, cinco años después de aumento mamario con implantes
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María Claudia Espinel-Bermúdez, Adolfo Ernesto Gómez-Díaz, Jorge Enrique Bayter-Marín, Héctor César Durán-Vega, Hiram Osiris González-Gutiérrez, Mayra Lorena Gutiérrez-Esparza, Lázaro Cárdenas-Camarena, Daniel Atl López-Fabila, and José Antonio Robles-Cervantes
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Organic Chemistry ,Biochemistry - Published
- 2022
10. Development and validation of the questionnaire to analyze the communication of nurses in nurse-patient therapeutic communication
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Gabriel Aguilera-Manrique, Verónica V. Márquez-Hernández, Lorena Gutiérrez-Puertas, Genoveva Granados-Gámez, M Carmen Rodríguez-García, Isabel María Sáez-Ruiz, and Manuel Luís Cibanal-Juan
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Medical education ,Psychometrics ,Communication ,030503 health policy & services ,Psychological intervention ,Nurses ,Reproducibility of Results ,Construct validity ,General Medicine ,Nurse patient ,Exploratory factor analysis ,03 medical and health sciences ,0302 clinical medicine ,Cronbach's alpha ,Surveys and Questionnaires ,Therapeutic communication ,Humans ,Nursing Care ,030212 general & internal medicine ,Situational ethics ,Nurse-Patient Relations ,0305 other medical science ,Psychology ,Reliability (statistics) - Abstract
Objective The purpose of this study was to develop and analyze the psychometric properties of an instrument that could analyze the communication of nursing professionals in nurse-patient therapeutic communication. Methods A literature review and a panel of experts were used (N = 10) to develop the questionnaire to analyze the communication of nurses in nurse-patient therapeutic communication. The final version of the questionnaire was composed of 49 items and applied to a convenience sample of 370 nurses. Results The construct validity was assessed by an exploratory factor analysis (EFA) and the reliability using Cronbach’s Alpha. Three dimensions were identified that determine therapeutic communication: professional, contextual and/or situational and patient. The Cronbach’s α total coefficient was 0.90, ranging from 0.71 to 0.81 for the dimensions. Conclusion The questionnaire to analyze the communication of nurses in nurse-patient therapeutic communication represents a valid and reliable questionnaire to measure nurses’ communication with patients in the clinical setting. Implications for practice The use of the questionnaire enables the analysis of elements that interfere with effective communication. This can assist in developing interventions to improve nurses’ therapeutic communication with patients.
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- 2022
11. Nucleo-Cytoplasmic Transport of ZIKV Non-Structural 3 Protein Is Mediated by Importin-α/β and Exportin CRM-1
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Luis Adrián De Jesús-González, Selvin Noé Palacios-Rápalo, José Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Carlos Noe Farfán-Morales, Carlos Daniel Cordero-Rivera, Bulmaro Cisneros, Ana Lorena Gutiérrez-Escolano, and Rosa María del Ángel
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Virology ,Insect Science ,Immunology ,Microbiology ,Genome Replication and Regulation of Viral Gene Expression - Abstract
Flaviviruses have a cytoplasmic replicative cycle, and crucial events, such as genome translation and replication, occur in the endoplasmic reticulum. However, some viral proteins, such as C, NS1, and NS5 from Zika virus (ZIKV) containing nuclear localization signals (NLSs) and nuclear export signals (NESs), are also located in the nucleus of Vero cells. The NS2A, NS3, and NS4A proteins from dengue virus (DENV) have also been reported to be in the nucleus of A549 cells, and our group recently reported that the NS3 protein is also located in the nucleus of Huh7 and C636 cells during DENV infection. However, the NS3 protease-helicase from ZIKV locates in the perinuclear region of infected cells and alters the morphology of the nuclear lamina, a component of the nuclear envelope. Furthermore, ZIKV NS3 has been reported to accumulate on the concave face of altered kidney-shaped nuclei and may be responsible for modifying other elements of the nuclear envelope. However, nuclear localization of NS3 from ZIKV has not been substantially investigated in human host cells. Our group has recently reported that DENV and ZIKV NS3 alter the nuclear pore complex (NPC) by cleaving some nucleoporins. Here, we demonstrate the presence of ZIKV NS3 in the nucleus of Huh7 cells early in infection and in the cytoplasm at later times postinfection. In addition, we found that ZIKV NS3 contains an NLS and a putative NES and uses the classic import (importin-α/β) and export pathway via CRM-1 to be transported between the cytoplasm and the nucleus. IMPORTANCE Flaviviruses have a cytoplasmic replication cycle, but recent evidence indicates that nuclear elements play a role in their viral replication. Viral proteins, such as NS5 and C, are imported into the nucleus, and blocking their import prevents replication. Because of the importance of the nucleus in viral replication and the role of NS3 in the modification of nuclear components, we investigated whether NS3 can be localized in the nucleus during ZIKV infection. We found that NS3 is imported into the nucleus via the importin pathway and exported to the cytoplasm via CRM-1. The significance of viral protein nuclear import and export and its relationship with infection establishment is highlighted, emphasizing the development of new host-directed antiviral therapeutic strategies.
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- 2023
12. ¿CÓMO OPTIMIZAR EL PROCESO DE ADQUISICIÓN DE COMPETENCIAS?
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Patricia Catalá, Lorena Gutiérrez, Carmen Ecija, Octavio Luque Reca, Rosa Martínez, Nuria Máximo, and Cecilia Peñacoba
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- 2022
13. The effect of treatment and coping on the quality of life in breast cancer patients: a moderated mediation model
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Lorena Gutiérrez-Hermoso, Paulina Quiroz-Friedman, Sofía Sánchez-Román, Lilian Velasco-Furlong, Elisabeth Berzal-Pérez, and Natasha Alcocer-Castillejos
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Coping (psychology) ,medicine.medical_treatment ,Breast Neoplasms ,Learned helplessness ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Moderated mediation ,Alexithymia ,Quality of life ,Surveys and Questionnaires ,Adaptation, Psychological ,medicine ,Humans ,business.industry ,030503 health policy & services ,Public Health, Environmental and Occupational Health ,medicine.disease ,Moderation ,Cross-Sectional Studies ,030220 oncology & carcinogenesis ,Quality of Life ,Female ,Hormone therapy ,0305 other medical science ,business ,Clinical psychology - Abstract
Breast cancer involves complicated emotional processes. One of the factors that impacts the psychological symptoms and decreases QoL is the side effects of treatment. The purpose of this study is to explore the effect of the main medical treatment (chemotherapy or hormone therapy) on the three domains of quality of life. For this, coping strategies were considered as psychological variables that mediate the relationship based on high or low alexithymia as a moderating variable. This study had a cross-sectional design. The participant sample comprised 129 women with breast cancer in early stage (I to III) (63 receiving chemotherapy and 66 hormone therapy) and were evaluated from September 2015 to September 2019. Physical, emotional and social functioning were measured by the Quality of Life Questionnaire for cancer patients (EORTC-QLQ-C30), coping strategies were measured by Mental Adjustment to Cancer Questionnaire (MAC) and alexithymia was evaluated by the Alexithymia Toronto Scale (TAS-20). Treatment had a significant negative effect on physical domain in both patients receiving chemotherapy and hormone therapy. Moderated mediation analysis show that this relationship was significant when it was mediated by helplessness. Furthermore, this model is only significant when there are high levels of alexithymia. No significant effect direct was found on emotional and social functioning of quality of life. Results confirmed that coping based on helplessness and stable emotional variables such as alexithymia can have an effect, mediator or moderator, respectively, in the decrease of the physical functionality of women with breast cancer. Our findings highlight the need to include psychological therapy to help patients alleviate their psychological state because it can affect their physical condition.
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- 2021
14. Symptoms in women with fibromyalgia after performing physical activity: the role of pain catastrophizing and disease impact
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Irene López-Gómez, Lilian Velasco, Lorena Gutiérrez, Carmen Écija, Patricia Catalá, and Cecilia Peñacoba
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Rheumatology ,General Medicine - Abstract
Introduction Walking is an effective treatment for symptoms’ management in patients with fibromyalgia. However, despite its benefits, fibromyalgia patients face a variety of obstacles that result in reduced ability to sustain physical exercise. The main goal of the study was to analyze the role of pain catastrophizing and fibromyalgia impact in the relationship between regular walking behavior and pain and fatigue experienced after a laboratory walking test. Method The study has an observational analytical laboratory design. A total of 100 women were contacted by the research team. Seventy-six women diagnosed with fibromyalgia aged 18 years and older (mean age = 55.05, SD = 7.69) participated. Results Significant correlations were found among regular walking behavior, pain catastrophizing, impact of fibromyalgia, pain intensity after walking, and fatigue intensity after walking. The serial multiple mediation analyses confirmed that pain catastrophizing and impact of fibromyalgia mediated the relationship between regular walking behavior and the level of pain (beta B = 0.044, 95% CI = [0.01–0.012]) and fatigue (beta B = 0.028, 95% CI = [0.01–0.08]) after the laboratory walking test. Also, the participants that walked less regularly experienced more pain and fatigue after the 6-Minute Walk Test. Conclusions Considering cognitive variables alongside the impact of fibromyalgia will help understand the inhibitors of engaging in physical activity. Therapeutic walking programs must be tailored to patients with fibromyalgia to reduce pain and fatigue related to physical activity and to promote better functioning and quality of life. Key Points• Regular walking behavior was associated with fibromyalgia impact, pain catastrophizing, and less pain and fatigue after physical activity.• When patients catastrophize pain, they usually interpret physical activity as threatening, which generates more pain and fatigue after doing exercise.• Therapeutic programs should be designed to reduce pain catastrophizing and fibromyalgia impact.
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- 2022
15. Mendelian Randomization Analysis of the Relationship Between Native American Ancestry and Gallbladder Cancer Risk
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Linda Zollner, Felix Boekstegers, Carol Barahona Ponce, Dominique Scherer, Katherine Marcelain, Valentina Gárate-Calderón, Melanie Waldenberger, Erik Morales, Armando Rojas, César Munoz, Bettina Müller, Javier Retamales, Gonzalo de Toro, Allan Vera Kortmann, Olga Barajas, María Teresa Rivera, Analía Cortés, Denisse Loader, Javiera Saavedra, Lorena Gutiérrez, Alejandro Ortega, Maria Enriqueta Bertrán, Leonardo Bartolotti, Fernando Gabler, Mónica Campos, Juan Alvarado, Fabricio Moisán, Loreto Spencer, Bruno Nervi, Daniel Carvajal, Héctor Losada, Mauricio Almau, Plinio Fernández, Jordi Olloquequi, Alice R. Carter, Juan Francisco Miquel Poblete, Bernabe Ignacio Bustos, Macarena Fuentes Guajardo, Rolando Gonzalez-Jose, Maria Cátira Bortolini, Victor Acuña-Alonzo, Carla Gallo, Andres Ruiz Linares, Francisco Rothhammer, and Justo Lorenzo Bermejo
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BackgroundA strong association between the proportion of Native American ancestry and the risk of gallbladder cancer (GBC) has been reported in observational studies. Chileans show the highest incidence of GBC worldwide, and the Mapuche are the largest Native American people in Chile. We set out to investigate the causal association between Native American Mapuche ancestry and GBC risk, and the possible mediating effects of gallstone disease and body mass index (BMI) on this association.MethodsMarkers of Mapuche ancestry were selected based on the informativeness for assignment measure and then used as instrumental variables in two-sample mendelian randomization (MR) analyses and complementary sensitivity analyses.ResultWe found evidence of a causal effect of Mapuche ancestry on GBC risk (inverse variance-weighted (IVW) risk increase of 0.8% for every 1% increase in Mapuche ancestry proportion, 95% CI 0.4% to 1.2%, p = 6.6×10-5). Mapuche ancestry was also causally linked to gallstone disease (IVW risk increase of 3.6% per 1% increase in Mapuche proportion, 95% CI 3.1% to 4.0%, p = 1.0×10-59), suggesting a mediating effect of gallstones in the relationship between Mapuche ancestry and GBC. In contrast, the proportion of Mapuche ancestry showed a negative causal effect on BMI (IVW estimate -0.006 kg/m2 per 1% increase in Mapuche proportion, 95% CI -0.009 to -0.003, p = 4.4×10-5).ConclusionsThe results presented here may have significant implications for GBC prevention and are important for future admixture mapping studies. Given that the association between Mapuche ancestry and GBC risk previously noted in observational studies appears to be causal, primary and secondary prevention strategies that take into account the individual proportion of Mapuche ancestry could be particularly efficient.
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- 2022
16. Does Pain Acceptance Contribute to Improved Functionality through Walking in Women with Fibromyalgia? Looking at Depressive Comorbidity
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Cecilia Peñacoba, Carmen Ecija, Lorena Gutiérrez, and Patricia Catalá
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Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,fibromyalgia ,functional limitation ,acceptance ,depression ,chronic pain ,comorbidity - Abstract
In the last decade, research has pointed to physical exercise as an effective treatment in fibromyalgia patients. Some studies have highlighted the role of acceptance and commitment therapy in optimizing the benefits of exercise in patients. However, given the high comorbidity in fibromyalgia, it is necessary to value its possible influence on the effect of certain variables, such as acceptance, on the benefits of treatments, such as physical exercise. Our aim is to test the role of acceptance in the benefits of walking over functional limitation, further assessing whether this model is equally valid, considering depressive symptomatology as an additional differential diagnosis. A cross-sectional study with a convenience sample through contacting Spanish fibromyalgia associations was carried out. A total of 231 women with fibromyalgia (mean age 56.91 years) participated in the study. Data were analyzed with the Process program (Model 4, Model 58, Model 7). The results highlight the role of acceptance as a mediator between walking and functional limitation (B = −1.86, SE = 0.93, 95% CI = [−3.83, −0.15]). This model, when depression is incorporated as a moderator, is significant only in patients without depression, revealing the need for personalized treatments in fibromyalgia, considering their most prevalent comorbidity.
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- 2023
17. Self-efficacy and commitment as psychological resources to adherence to walking despite chronic pain. A study on women with fibromyalgia
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Patricia Catala, Lorena Gutiérrez, Carmen Ecija, and Cecilia Peñacoba
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Psychiatry and Mental health ,Social Psychology ,Applied Psychology - Published
- 2021
18. Escape Rooms as a Clinical Evaluation Method for Nursing Students
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Verónica V. Márquez-Hernández, Guadalupe Molina-Torres, Miguel Rodriguez-Arrastia, Carmen Ropero-Padilla, Pablo Román-López, and Lorena Gutiérrez-Puertas
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Nursing (miscellaneous) ,030504 nursing ,clinical evaluation ,education ,030208 emergency & critical care medicine ,clinical skills ,Education ,03 medical and health sciences ,escape room ,0302 clinical medicine ,Nursing ,Modeling and Simulation ,gamification ,0305 other medical science ,Psychology ,Clinical evaluation ,Clinical skills ,nursing students - Abstract
Background: There are currently no studies available about the possible use of gamification in the evaluation of nursing students’ clinical skills. The purpose of this study was to understand the gameful experience and satisfaction of nursing students in the evaluation of their clinical skills using an escape room. Methods: A quasiexperimental study was carried out. The participants were divided into an experimental group (escape room) and a control group. Results: The experimental group had higher than average scores in all dimensions of the gameful experience scale, except in the dimension of negative effects. Conclusions: Escape rooms are a useful tool for the evaluation of nursing students compared with using the objective structured clinical evaluation.
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- 2020
19. Systematic review of measurement properties of self-reported instruments for evaluating therapeutic communication
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Gabriel Aguilera-Manrique, Lorena Gutiérrez-Puertas, Verónica V. Márquez-Hernández, Genoveva Granados-Gámez, Isabel María Sáez-Ruiz, and José Luis Ybarra-Sagarduy
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Consensus ,Psychometrics ,030504 nursing ,020205 medical informatics ,Communication ,Relational communication ,02 engineering and technology ,CINAHL ,Therapeutic relationship ,03 medical and health sciences ,Nursing ,0202 electrical engineering, electronic engineering, information engineering ,Therapeutic communication ,Humans ,Self Report ,0305 other medical science ,Psychology ,General Nursing - Abstract
A systematic review was carried out to examine measurement tools of therapeutic relational communication, using an electronic search of the following databases: PubMed, LILACS, CINAHL, CiberIndex, Scielo and Scopus. The project followed the guidelines of Preferred Reporting Items for Systematic Reviews and Meta-Analysis and the protocol recommended by the Consensus-based Standards for the selection of health Measurement Instruments (COSMIN) panel. Thirteen instruments were included in the review. and their measurement properties were tested. The most common communication elements found in the measuring instruments were: empathy, respect, listening, contact, communicative competence, communication quality, and communication skills. Studies that used a theoretical foundation were based on patient-centered humanistic models. Empathy, respect, controlled contact, and other basic elements of interpersonal communication were most commonly found among the measuring regents. In conclusion, this review analyzed the theoretical foundations of the components of instruments used to measure therapeutic relational communication in the nursing field.
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- 2020
20. Undergraduate nurses' perception of the nursing practice environment in university hospitals: A cross‐sectional survey
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Verónica V. Márquez-Hernández, Genoveva Granados-Gámez, Lorena Gutiérrez-Puertas, Gabriel Aguilera-Manrique, and Mª Carmen Rodríguez-García
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Medical education ,Data collection ,030504 nursing ,Psychometrics ,Leadership and Management ,030503 health policy & services ,Staffing ,Nurses ,Reproducibility of Results ,Construct validity ,Exploratory factor analysis ,Hospitals, University ,03 medical and health sciences ,Cross-Sectional Studies ,Cronbach's alpha ,Surveys and Questionnaires ,Scale (social sciences) ,Humans ,Perception ,Workplace ,0305 other medical science ,Nursing management ,Psychology - Abstract
Objective To describe the psychometric properties of the Practice Environment Scale of the Nursing Work Index (PES-NW) among undergraduate nurses and to compare their perceptions of the nursing practice environment by academic year and unit type. Background Despite the fact that nursing students develop most of their undergraduate learning and training in the nursing practice environment, their perception about it has not been considered to date. Methods The psychometric properties were analysed on a sample of 180 undergraduate nurses. Data collection was carried out in 2018. Data were analysed using percentages, frequencies, mean, standard deviation and Mann-Whitney U test. Factor structure was evaluated with exploratory factor analysis, and reliability was evaluated with Cronbach's alpha. Results Psychometric analysis showed an adequate construct validity and reliability for the PES-NWI. Cronbach's Alpha was 0.884. Undergraduate nurses perceived most hospitals as favourable, scoring the 'Nurse-physician relationships' factor highest and 'Staffing and resource adequacy' factor lowest. Conclusion The PES-NWI is a valid and reliable instrument that could be applied in future research to explore nursing students' perceptions of the nursing practice environment. Implications for nursing management Nurse managers are responsible for supervising and ensuring that the nursing practice environment at university hospitals meets the necessary criteria to support the workplace learning of undergraduate nurses.
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- 2020
21. Trailblazers, Reciprocity, and Doctoral Education: The Pursuit of Critical Race Praxis and Survivance among Doctoral Students of Color
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Tuesda S. Roberts, ThedaMarie Gibbs Grey, Raven Jones Stanbrough, and Lorena Gutiérrez
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Praxis ,media_common.quotation_subject ,Critical race theory ,05 social sciences ,050301 education ,Survey research ,Racism ,Education ,Race (biology) ,Critical theory ,Reciprocity (social psychology) ,0502 economics and business ,Pedagogy ,ComputingMilieux_COMPUTERSANDEDUCATION ,Sociology ,050207 economics ,Doctoral education ,0503 education ,ComputingMilieux_MISCELLANEOUS ,media_common - Abstract
This study utilized a mixed-methods, survey research design to explore the experiences and motivations of Students of Color who pursue doctoral studies in colleges or departments of education and t...
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- 2020
22. Association between quality of life and positive coping strategies in breast cancer patients
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Cecilia Peñacoba, Lilian Velasco, Patricia Catala, Sofía Sánchez-Román, Natasha Alcocer Castillejos, Paulina Quiroz Friedman, and Lorena Gutiérrez Hermoso
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Adult ,Gerontology ,Emotions ,Breast Neoplasms ,Persona ,Emotional Adjustment ,World health ,03 medical and health sciences ,0302 clinical medicine ,Quality of life (healthcare) ,Breast cancer ,Surveys and Questionnaires ,Adaptation, Psychological ,Humans ,Medicine ,030212 general & internal medicine ,Association (psychology) ,Mexico ,Aged ,Female population ,030219 obstetrics & reproductive medicine ,business.industry ,Positive coping ,General Medicine ,Middle Aged ,medicine.disease ,Cross-Sectional Studies ,Mental Health ,Spain ,Quality of Life ,Female ,business ,Stress, Psychological - Abstract
According to the World Health Organization, breast cancer is one of the most prevalent cancers in the Hispanic female population, and it carries a great psychological impact. Acceptance and personal learning derived from the individual's life experiences and understanding of their emotional state has been suggested as an adaptive profile toward the patient's perception of the disease. This study aims to explore which psychological variables are related to breast cancer patients' perception of their quality of life. A cross-sectional, correlational, and non-probabilistic study was performed on 113 women diagnosed with stage 1-3 breast cancer in Mexico and Spain, based on self-reporting through the Mental Adjustment to Cancer Scale, the Positive and Negative Affect Scale, and the EORTC Quality of Life Questionnaire. Recruitment was conducted from March 2015 to April 2018. Through multiple regression analysis, the study found that fighting spirit and positive affect explained 34.2% of quality of life variance F (2, 110) = 30.14
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- 2020
23. The Effect of Cell Phones on Attention and Learning in Nursing Students
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Gabriel Aguilera-Manrique, Vanesa Gutiérrez-Puertas, Verónica V. Márquez-Hernández, Lorena Gutiérrez-Puertas, and Genoveva Granados-Gámez
- Subjects
Male ,Organizational Behavior and Human Resource Management ,020205 medical informatics ,Strategy and Management ,Pharmaceutical Science ,02 engineering and technology ,Young Adult ,03 medical and health sciences ,Nursing ,Phone ,Surveys and Questionnaires ,Drug Discovery ,0202 electrical engineering, electronic engineering, information engineering ,Humans ,Learning ,Attention ,Marketing ,Pharmacology ,Class (computer programming) ,030504 nursing ,Nomophobia ,Academic Training ,Female ,Students, Nursing ,0305 other medical science ,Psychology ,Cell Phone - Abstract
The use of cell phones can have a negative effect on nursing students' academic performance. The purpose of this study was to identify the effects that problematic cell phone use and nomophobia have on learning in nursing students. A quasi-experimental study was carried out among nursing students, with a control group (n = 61, 49.2%) and an experimental group (n = 63, 50.8%). The experimental group left their cell phones, in sound or vibration mode, as they normally kept them, on a table located in the corner of the classroom. Both groups showed higher-than-average levels of nomophobia at the beginning of the class. As for problematic cell phone use and attention, it was found that there were statistically significant differences in both cases (U = 1355.500, Z = -2.830, P = .005; U = 1449.000, Z = -2.363, P = .018, respectively), and the experimental group had higher average scores, as well as in those related to knowledge. A relationship has been found between nomophobia and problematic cell phone use. Students who do not have access to their cell phones during class perceive more problematic cell phone usage, in addition to paying closer attention during class. Because of this, it is important to create policies and norms restricting cell phone use during all types of academic training among nursing students.
- Published
- 2020
24. Identification of Circulating lncRNAs Associated with Gallbladder Cancer Risk by Tissue-Based Preselection, Cis-eQTL Validation, and Analysis of Association with Genotype-Based Expression
- Author
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Alice Blandino, Dominique Scherer, Trine B. Rounge, Sinan U. Umu, Felix Boekstegers, Carol Barahona Ponce, Katherine Marcelain, Valentina Gárate-Calderón, Melanie Waldenberger, Erik Morales, Armando Rojas, César Munoz, Javier Retamales, Gonzalo de Toro, Olga Barajas, María Teresa Rivera, Analía Cortés, Denisse Loader, Javiera Saavedra, Lorena Gutiérrez, Alejandro Ortega, Maria Enriqueta Bertrán, Fernando Gabler, Mónica Campos, Juan Alvarado, Fabrizio Moisán, Loreto Spencer, Bruno Nervi, Daniel E. Carvajal-Hausdorf, Héctor Losada, Mauricio Almau, Plinio Fernández, Ivan Gallegos, Jordi Olloquequi, Macarena Fuentes-Guajardo, Rolando Gonzalez-Jose, Maria Cátira Bortolini, Carla Gallo, Andres Ruiz Linares, Francisco Rothhammer, and Justo Lorenzo Bermejo
- Subjects
molecular phenotypes ,Cancer Research ,Human genome ,eQTLs ,EQTLs ,lncRNAs ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Gallbladder cancer ,Genoma humà ,Molecular phenotypes ,gallbladder cancer ,Oncology ,genetic association study ,RNA ,Eqtls ,Gallbladder Cancer ,Genetic Association Study ,Lncrnas ,Molecular Phenotypes ,LncRNAs ,Càncer ,RC254-282 ,Genetic association study ,Cancer - Abstract
Long noncoding RNAs (lncRNAs) play key roles in cell processes and are good candidates for cancer risk prediction. Few studies have investigated the association between individual genotypes and lncRNA expression. Here we integrate three separate datasets with information on lncRNA expression only, both lncRNA expression and genotype, and genotype information only to identify circulating lncRNAs associated with the risk of gallbladder cancer (GBC) using robust linear and logistic regression techniques. In the first dataset, we preselect lncRNAs based on expression changes along the sequence “gallstones → dysplasia → GBC”. In the second dataset, we validate associations between genetic variants and serum expression levels of the preselected lncRNAs (cis-lncRNA-eQTLs) and build lncRNA expression prediction models. In the third dataset, we predict serum lncRNA expression based on individual genotypes and assess the association between genotype-based expression and GBC risk. AC084082.3 and LINC00662 showed increasing expression levels (p-value = 0.009), while C22orf34 expression decreased in the sequence from gallstones to GBC (p-value = 0.04). We identified and validated two cis-LINC00662-eQTLs (r2 = 0.26) and three cis-C22orf34-eQTLs (r2 = 0.24). Only LINC00662 showed a genotyped-based serum expression associated with GBC risk (OR = 1.25 per log2 expression unit, 95% CI 1.04–1.52, p-value = 0.02). Our results suggest that preselection of lncRNAs based on tissue samples and exploitation of cis-lncRNA-eQTLs may facilitate the identification of circulating noncoding RNAs linked to cancer risk.
- Published
- 2022
25. The Caliciviridae Family
- Author
-
Carlos Emilio Miguel-Rodríguez, Yoatzin Peñaflor-Téllez, and Ana Lorena Gutiérrez-Escolano
- Subjects
Genetics ,Biology ,biology.organism_classification ,Caliciviridae - Published
- 2022
26. Patient’s Perception and Real Execution of Walking as Physical Exercise: Looking at Self-Efficacy as a Key Variable in Adherence in Patients with Fibromyalgia
- Author
-
Ana Myriam Lavín Pérez, Lorena Gutiérrez, Carmen ECIJA-GALLARDO, Cecilia Peñacoba, Patricia CATALA, Alexander Gil Arias, and Daniel Collado-Mateo
- Subjects
Fluid Flow and Transfer Processes ,fibromyalgia ,walking ,self-efficacy ,perception of walking ,accelerometry ,mediation analysis ,Process Chemistry and Technology ,General Engineering ,General Materials Science ,Instrumentation ,Computer Science Applications - Abstract
Adherence to physical exercise is especially low in patients with fibromyalgia, and there is a tendency to overestimate its performance. This study explores the relationship between perceived and actual walking behavior in patients with fibromyalgia, including self-efficacy as a mediating variable. A prospective study with two time points (with one week between them) was carried out on 132 women diagnosed with fibromyalgia. Self-efficacy towards exercise was assessed at the first time point, and perceived intensity of physical activity and “objective” walking amount (accelerometer) in relation to the previous week were assessed at the second point. Physical and emotional symptoms and the consumption of drugs were also evaluated as covariates. The results show that perception of vigorous-intensity exercise was related to walking (accelerometer) (effort greater than 6 METs). This relationship was explained by the mediating effect of self-efficacy and was not interfered with either by the symptoms or by the consumption of drugs. High self-efficacy regarding physical exercise was directly related to walking behavior despite patients’ interpretation of this activity as a vigorous physical exercise. In intervention and rehabilitation programs, it would be interesting to take into account and promote the self-efficacy of patients in relation to physical exercise in order to maintain adherence to walking.
- Published
- 2023
27. Pathological Cycle between Pain, Insomnia, and Anxiety in Women with Fibromyalgia and its Association with Disease Impact
- Author
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Cecilia Peñacoba, Carmen ECIJA-GALLARDO, Lorena Gutiérrez, and Patricia CATALA
- Subjects
pain ,anxiety ,insomnia ,fibromyalgia ,mediation analysis ,disability studies ,behavioral medicine ,Medicine (miscellaneous) ,General Biochemistry, Genetics and Molecular Biology - Abstract
Background: Pain, sleep disturbances, and mood disorders are considered common symptoms of fibromyalgia (FM). However, the interactions that are established between them and the implication that this has in the disease are not clear. The main objective of this study is to clarify the relationships established between insomnia, pain intensity and anxiety in women with FM. Additionally, the effect that the indicated pathological cycle between pain, insomnia and anxiety may have on the impact of the disease in these patients is explored. Methods: A total of 228 women diagnosed with FM participated in this study (mean age = 56.99 years, SD = 10.35). Measurements were conducted at two time points. Initially, the women completed self-report questionnaires about anxiety (The Hospital Anxiety and Depression Scale; HADS), sleep problems (The Insomnia Severity Index; ISI) and pain intensity (Brief Pain Inventory; BPI), and a week later, they answered questions about the impact of fibromyalgia (Fibromyalgia Impact Questionnaire- Revised; FIQ-R). For data analysis, models 4 and 6 of the Macro Process for SPSS were used. Results: Insomnia predicts higher levels of pain, which in turn predicts higher levels of anxiety, which in turn predicts a higher impact of fibromyalgia (B = 2.76, SE = 1.10, 95% CI = [0.79,5.11]). No significant results were found for the other interactions between the variables. Conclusions: Due to the clinical and scientific relevance of the insomnia–pain–anxiety pathological cycle and given the impact it has on FM, it is especially relevant to develop programs for patients with fibromyalgia based mainly on improving sleep quality.
- Published
- 2023
28. The Role of Self-Efficacy and Activity Patterns in the Physical Activity Levels of Women with Fibromyalgia
- Author
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Ana Myriam Lavín Pérez, Lorena Gutiérrez, Carmen ECIJA-GALLARDO, Cecilia Peñacoba, Patricia CATALA, Alexander Gil Arias, and Daniel Collado-Mateo
- Subjects
General Immunology and Microbiology ,self-efficacy ,accelerometry ,sedentarism ,chronic pain ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology - Abstract
Keeping high levels of physical activity is a challenge among chronic patients. In this regard, self-efficacy has been identified as a crucial variable to reduce sedentarism and physical inactivity in women with fibromyalgia. The current study aimed to evaluate the associations among objective physical activity levels, self-efficacy, activity patterns, and the impact of the disease, as well as to compare those variables between women with fibromyalgia with different self-efficacy levels. For this purpose, in this cross-sectional study, the physical activity levels of 123 women with fibromyalgia were assessed by accelerometers, together with self-efficacy, the impact of the disease, and activity patterns. Results revealed that self-efficacy for light or moderate physical activity was directly related to light (p < 0.01), moderate (p < 0.01), and vigorous physical activity (p < 0.05), as well as inversely related to sedentary time (p < 0.01). Moreover, the main differences were observed between those with low self-efficacy levels and the rest of the sample, while there were no differences between the high and the medium self-efficacy groups (p > 0.05). Thus, self-efficacy for walking and light physical activity seems to be more relevant than self-efficacy for moderate and vigorous physical activity to achieve higher levels of physical activity.
- Published
- 2023
29. The Feline Calicivirus Leader of the Capsid Protein Has the Functional Characteristics of a Viroporin
- Author
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Yoatzin Peñaflor-Téllez, Bibiana Chávez-Munguía, Anel Lagunes-Guillén, Lizbeth Salazar-Villatoro, and Ana Lorena Gutiérrez-Escolano
- Subjects
feline calicivirus ,LC ,viroporin ,disulfide bonds ,Infectious Diseases ,Virology ,Cats ,Escherichia coli ,Animals ,Capsid Proteins ,Disulfides ,Caliciviridae Infections ,Calicivirus, Feline ,Cell Line ,Viroporin Proteins - Abstract
The leader of the capsid (LC) protein is exclusive to the Vesivirus genus, and it is needed for successful feline calicivirus (FCV) replication, as well as an efficient apoptosis induction through the mitochondrial pathway. In this work, we aimed to determine if the LC protein from the FCV is a viroporin. Although lacking in a transmembrane domain or an amphipathic helix, the LC protein from the FCV is toxic when expressed in bacteria and it oligomerizes through disulfide bonds, which are both key characteristics of viroporins. An electron microscopy analysis of LC-expressing E. coli cells suggest that the protein induces osmotic stress. Moreover, we found that the previously studied C40A LC mutant, that fails to induce apoptosis and that hinders the replication cycle, also oligomerizes but it has a reduced toxicity and fails to induce osmotic stress in bacteria. We propose that the LC protein is a viroporin that acts as a disulfide bond-dependent antimicrobial peptide, similar to the Ebola virus delta peptide.
- Published
- 2021
30. APLICACIÓN DEL APRENDIZAJE BASADO EN PROYECTOS (ABP) EN LAS DIFERENTES MODALIDADES DOCENTES
- Author
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Patricia Catalá Mesón, Carlos María Alcover de la Hera, Lorena Gutiérrez Hermoso, and Cecilia Peñacoba Puente
- Published
- 2021
31. ¿SE ENSEÑA 'LO MISMO' EMPLEANDO MODOS DIFERENTES?
- Author
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Cecilia Peñacoba Puente, Lorena Gutiérrez Hermoso, Patricia Catalá Mesón, and Lilian Velasco Furlong
- Published
- 2021
32. EL USO DEL FLIPPED CLASSROOM ONLINE COMO TÉCNICA DE APRENDIZAJE ACTIVO EN EL FOMENTO DE COMPETENCIAS EN ESTUDIANTES DE PSICOLOGÍA
- Author
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Lorena Gutiérrez Hermoso, Patricia Catalá Mesón, Lilian Velasco Furlong, and Cecilia Peñacoba Puente
- Published
- 2021
33. Flavivirus infections induce a Golgi stress response in vertebrate and mosquito cells
- Author
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Mercedes Viettri, José L. Zambrano, Romel Rosales, Gerson I. Caraballo, Ana Lorena Gutiérrez-Escolano, and Juan E. Ludert
- Subjects
Multidisciplinary ,Mesocricetus ,Science ,Flavivirus ,viruses ,Golgi Apparatus ,Viral Nonstructural Proteins ,Virus Replication ,Microbiology ,Article ,Flavivirus Infections ,Culicidae ,Virology ,Chlorocebus aethiops ,Vertebrates ,Medicine ,Animals ,Pathogens ,Vero Cells ,Cells, Cultured - Abstract
The stress of the Golgi apparatus is an autoregulatory mechanism that is induced to compensate for greater demand in the Golgi functions. No examples of Golgi stress responses due to physiological stimuli are known. Furthermore, the impact on this organelle of viral infections that occupy the vesicular transport during replication is unknown. In this work, we evaluated if a Golgi stress response is triggered during dengue and Zika viruses replication, two flaviviruses whose replicative cycle is heavily involved with the Golgi complex, in vertebrate and mosquito cells. Using GM-130 as a Golgi marker, and treatment with monensin as a positive control for the induction of the Golgi stress response, a significant expansion of the Golgi cisternae was observed in BHK-21, Vero E6 and mosquito cells infected with either virus. Activation of the TFE3 pathway was observed in the infected cells as indicated by the translocation from the cytoplasm to the nucleus of TFE3 and increased expression of pathway targeted genes. Of note, no sign of activation of the stress response was observed in CRFK cells infected with Feline Calicivirus (FCV), a virus released by cell lysis, not requiring vesicular transport. Finally, dilatation of the Golgi complex and translocation of TFE3 was observed in vertebrate cells expressing dengue and Zika viruses NS1, but not NS3. These results indicated that infections by dengue and Zika viruses induce a Golgi stress response in vertebrate and mosquito cells due to the increased demand on the Golgi complex imposed by virion and NS1 processing and secretion.
- Published
- 2021
34. Evaluation of Urinary Catheterization Competency and Self-Efficacy in Nursing Students Using the Flipped Classroom Approach
- Author
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Gabriel Aguilera-Manrique, Lorena Gutiérrez-Puertas, Vanesa Gutiérrez-Puertas, Blanca Ortiz-Rodríguez, and Verónica V. Márquez-Hernández
- Subjects
Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,Humans ,Students, Nursing ,Problem-Based Learning ,Clinical Competence ,Urinary Catheterization ,flipped classroom ,nursing students ,self-efficacy ,skills ,urinary catheterization ,Self Efficacy - Abstract
Urinary catheterization is a complex procedure. Traditional teaching in classroom, such as using a simulator, is not enough to guarantee the necessary learning outcomes. It is therefore necessary to implement new active learning approaches such as the flipped classroom. The aim of this study was to examine the effect of the use of the flipped classroom on the level of self-efficacy and the assessment of clinical competencies in nursing students performing the urinary catheterization procedure. A quasi-experimental study of a single group with pre-/post-testing measures. A convenience sample of 139 nursing students. A flipped classroom approach was implemented. Measurements were taken pre and post intervention. This study followed the consolidated criteria for quality of nonrandomized evaluations studies (TREND) checklist. Regarding self-efficacy, statistically significant differences were found between the pre-test and post-test (Z = −14.453; p < 0.05). With respect to knowledge level, statistically significant differences were found when comparing pre-/post-test results (Z = −14,480; p < 0.05). Furthermore, statistically significant differences were found on the ability scale depending on pre-/post-test (Z = −10.277; p < 0.005); in all cases, obtaining a higher score in the post-test. The flipped classroom could be an effective educational tool for the development of clinical skills, specifically urinary catheterization. This method has been shown to improve the knowledge, skills, and self-efficacy in urinary catheterization of nursing students.
- Published
- 2022
35. Intervention for Alleviating Clinical Inertia in the Management of Urinary Incontinence
- Author
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Genoveva Granados-Gámez, Lorena Gutiérrez-Puertas, Gabriel Aguilera-Manrique, Verónica V. Márquez-Hernández, Consuelo Artero-López, and María Teresa Estevez-Morales
- Subjects
Adult ,medicine.medical_specialty ,Health Knowledge, Attitudes, Practice ,Nurses ,Urinary incontinence ,03 medical and health sciences ,Nursing care ,0302 clinical medicine ,Intervention (counseling) ,medicine ,Humans ,030212 general & internal medicine ,Unlicensed assistive personnel ,Advanced and Specialized Nursing ,030504 nursing ,Inpatient care ,business.industry ,Medical record ,Middle Aged ,Checklist ,Test (assessment) ,Medical–Surgical Nursing ,Urinary Incontinence ,Spain ,Physical therapy ,Female ,Nursing Care ,medicine.symptom ,0305 other medical science ,business - Abstract
PURPOSE The purpose of this study was to determine whether clinical inertia exists in the care of patients with urinary incontinence and to test the effects of an educational intervention designed to alleviate inertia related to care of urinary incontinence. DESIGN Single-group, pretest/posttest design. SUBJECTS AND SETTING The sample comprised 132 participants practicing in 6 medical-surgical inpatient care units at an urban hospital in Almeria, Spain; 66 (50%) were RNs, and all other participants were unlicensed assistive personnel. Most participants (87.9%) were female; their average age was 43.99 years (SD = 7.85 years). METHODS An educational intervention (online course) was provided. The main objective of the course was application of evidence-based knowledge for prevention and management of urinary incontinence in hospitalized patients. The course program comprised 3 didactic units: (1) introduction and evaluation of the patient with urinary incontinence, (2) care plans, and (3) instructions and rational use of absorbent products. Data collection included demographic and professional characteristics of respondents, desired results when caring for patients with incontinence, a medical record checklist, an occupational environment and satisfaction scale, and an assessment record of knowledge/skills and training activity satisfaction. RESULTS After comparing the pre- and posttest scores of knowledge on urinary incontinence, significant differences were found before and after the intervention (z = -14.113, P = .000). Specifically, analysis revealed differences in caring actions related to urinary incontinence (z = -14.248, P = .000) and differences in direct observation of urinary incontinence practice following the intervention (z = -14.326, P = .000). CONCLUSIONS Our findings indicate existence of inertia in nursing care for hospitalized patients with urinary incontinence. The educational intervention improved knowledge, observable nursing care activities related to incontinence management, and improved documentation of care.
- Published
- 2021
36. Flavivirus Infection Induce a Golgi Stress Response in Vertebrate and Mosquito Cells
- Author
-
José L. Zambrano, Mercedes Viettri, Ana Lorena Gutiérrez-Escolano, Romel Rosales-Ramirez, and Juan E. Ludert
- Subjects
Fight-or-flight response ,symbols.namesake ,Flavivirus ,biology ,viruses ,biology.animal ,symbols ,Vertebrate ,Golgi apparatus ,biology.organism_classification ,Cell biology - Abstract
The stress of the Golgi apparatus is an autoregulatory mechanism that is induced to compensate for greater demand in the Golgi functions. Few examples of Golgi stress responses due to physiological stimuli are known. Furthermore, the impact on this organelle of viral infections that occupy the vesicular transport during replication is unknown. In this work, we evaluated if a Golgi stress response is triggered during dengue and Zika viruses replication, two flaviviruses whose replicative cycle is heavily involved with the Golgi complex, in vertebrate and mosquito cells. Using GM-130 as a Golgi marker, and treatment with monensin as a positive control for the induction of the Golgi stress response, a significant expansion of the Golgi cisternae was observed in BHK-21, Vero E6 and mosquito cells infected with either virus. Activation of the TFE3 pathway was observed in the infected cells as indicated by the translocation from the cytoplasm to the nucleus of TFE3. Of note, no sign of activation of the stress response was observed in CRFK cells infected with Feline Calicivirus (FCV), a virus released by cell lysis, not requiring vesicular transport. Finally, dilatation of the Golgi complex and translocation of TFE3 was observed in vertebrate cells expressing dengue and Zika viruses NS1, but not NS3. These results indicated that infections by dengue and Zika viruses induce a Golgi stress response in vertebrate and mosquito cells due to the increased demand on Golgi complex imposed by virion and NS1 processing and secretion.
- Published
- 2021
37. Urgencias médicas en cirugía general
- Author
-
Cristian Villagómez, Daniela Montenegro, Jorge Guadalupe, Silvana Robles, Salomé Castillo, Lorena Gutiérrez García, Paola Álvarez, Alexander Mariño, David Jativa, Lisseth Moreira, José Alfonso Abecia Martínez, and Camila Félix
- Published
- 2021
38. Undergraduate Nursing Students' Attitudes, Knowledge, and Skills Related to Children's Environmental Health
- Author
-
Daniel Puente-Fernández, Lorena Gutiérrez-Puertas, Ana María Porcel-Gálvez, Ana Lavedán-Santamaría, Carmen Álvarez-Nieto, Vanesa Cantón-Habas, Isabel M. López-Medina, Raquel Sarabia-Lavín, Inmaculada López-Leiva, Cristina Álvarez-García, and Sebastián Sanz-Martos
- Subjects
Adult ,Male ,Health Knowledge, Attitudes, Practice ,Adolescent ,020205 medical informatics ,Undergraduate nursing ,Cross-sectional study ,education ,MEDLINE ,Good attitude ,02 engineering and technology ,Child health ,Education ,Young Adult ,03 medical and health sciences ,Multivariate analysis of variance ,Environmental health ,0202 electrical engineering, electronic engineering, information engineering ,Humans ,Child ,Curriculum ,General Nursing ,030504 nursing ,Child Health ,Education, Nursing, Baccalaureate ,Cross-Sectional Studies ,Multicenter study ,Female ,Students, Nursing ,0305 other medical science ,Psychology ,Environmental Health - Abstract
Background: Nurses are in a privileged position to detect environmental risks among children. The objective was to determine attitudes, knowledge, and skills related to children's environmental health in undergraduate nursing students. Method: A cross-sectional study was designed in eight universities ( n = 2,462) from September 2017 to June 2018. Descriptive values and multivariate analysis of variance were calculated using three questionnaires. Results: Of the total 2,462 students in the sample, 2,155 had a good attitude regarding addressing children's environmental health problems, 501 had good knowledge, and 1,162 had good skills. Students who had attended a session on sustainability more than 3 months prior had a better attitude (9.93%), knowledge (11.16%), and skills (3.82%). Second course students and men had better environmental competency ( p < .001). Conclusion: Undergraduate nursing students have good attitudes; however, they lack knowledge and skills. There is a need to include children's environmental health in nursing curricula. [ J Nurs Educ. 2019;58(7):401–408.]
- Published
- 2019
39. Adaptation of the Spanish version of the Self-Efficacy Consumption of Fruit and Vegetables Scale
- Author
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Lorena Gutiérrez-Puertas, Verónica V. Márquez-Hernández, Tania Alcaraz-Córdoba, Vanesa Gutiérrez-Puertas, Genoveva Granados-Gámez, and Gabriel Aguilera-Manrique
- Subjects
Nutrition and Dietetics ,Medicine (miscellaneous) - Abstract
The objective of this study was to analyse the psychometric properties of the Spanish version of the Self-Efficacy Consumption of Fruit and Vegetables Scale among the Spanish population. The sample was made up of 506 subjects who were selected through a convenience sample. The original version was translated from English to Spanish and later was translated back into English, by different translators. Upon translating, not only did the translators keep in mind the grammatical and linguistic aspects of the text, but also the cultural aspects. In order to validate the content of the Scales, the adapted and agreed-upon version of the scale was evaluated by a panel of experts, in order to obtain the final version. Data collection took place between March and September 2017.The internal consistency was tested using Chronbach’s alpha. For the analysis of the structural validity, a factor analysis was carried out on the Self-Efficacy Consumption of Fruit and Vegetables Scale-Spanish version. Kaiser-Meyer Olkin and Bartlett’s test of sphericity (0.90) confirmed that the analysis was suitable. A two-factor structure was confirmed, as in the original scale. The value of Chronbach’s α for the total questionnaire was 0.877, which shows a good internal consistency. In the analysis of the reliability test-retest, a correlation value of 0.79 (p
- Published
- 2019
40. Annexin A2 associates to feline calicivirus RNA in the replication complexes from infected cells and participates in an efficient viral replication
- Author
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Adrian Trujillo-Uscanga, Juan Carlos Santos-Valencia, Anel Lagunes-Guillén, Clotilde Cancio-Lonches, Ana Lorena Gutiérrez-Escolano, and Beatriz Alvarado-Hernández
- Subjects
Scaffold protein ,Cancer Research ,viruses ,Viral Nonstructural Proteins ,Virus Replication ,Mass Spectrometry ,Cell Line ,03 medical and health sciences ,Cytopathogenic Effect, Viral ,Annexin ,Virology ,Animals ,Annexin A2 ,RNA, Double-Stranded ,030304 developmental biology ,Cytopathic effect ,0303 health sciences ,Feline calicivirus ,biology ,030306 microbiology ,RNA ,Viral Load ,biology.organism_classification ,RNA silencing ,Infectious Diseases ,Viral replication ,Host-Pathogen Interactions ,Cats ,RNA, Viral ,Calicivirus, Feline ,Protein Binding - Abstract
Cellular proteins have been identified to participate in calicivirus replication in association with viral proteins and/or viral RNAs. By mass spectrometry from pull-down assays, we identified several cellular proteins bound to the feline calicivirus (FCV) genomic RNA; among them the lipid raft-associated scaffold protein Annexin (Anx) A2. AnxA2 colocalizes with FCV NS6/7 protein and with the dsRNA in infected cells; moreover, it was found associated with the viral RNA in the membrane fraction corresponding to the replication complexes (RCs), suggesting its role during FCV replication. AnxA2-knockdown from CrFK cells prior to infection with FCV caused a delay in the cytopathic effect, a strong reduction of viral non-structural proteins and dsRNA production, and a decrease of FCV yield in both cell-associated and supernatant fractions. Taken together, these results indicate that AnxA2 associates to the genomic RNA of FCV and is required for an efficient FCV replication.
- Published
- 2019
41. Mejora de la funcionalidad física y emocional tras un programa de intervención multicomponente en mujeres con cáncer de mama
- Author
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Lilian Velasco Furlong, Paulina Quiroz Friedman, Elisabeth Berzal Pérez, Natasha Alcocer Castillejos, Lorena Gutiérrez Hermoso, Lorena Salas Costumero, Cecilia Peñacoba Puente, Sofía Sánchez Román, and Patricia Catalá Mesón Doi: https
- Abstract
El cáncer de mama es un tumor maligno y su impacto psicológico ha sido demostrado ampliamente. Tal afección hace evidente la necesidad de trabajar desde la multidisciplinariedad para mejorar la sintomatología afectiva y la limitación funcional de estas pacientes. En el presente trabajo se implementa una intervención psicológica en 69 mujeres con cáncer de mama siendo 19 españolas y 50 mexicanas que fueron divididas en grupo experimental (N=41) y control (N=28). El grupo experimental realizó un programa de 4 módulos: 1) manejo de estrés (incorporando ejercicios de consciencia corporal, yoga y mindfulness), 2) aceptación a la enfermedad, 3) regulación emocional, y 4) apoyo social. Los resultados revelaron una mejora de la calidad de vida y de la imagen corporal, disminución de la alexitimia y la sintomatología física y emocional. Estos datos confirman que el programa ofrece resultados beneficiosos para las mujeres con cáncer de mama incorporando el ejercicio físico junto con las técnicas psicológicas.
- Published
- 2019
42. Educación ambiental en el desierto: un esfuerzo proactivo
- Author
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Mélida Gutiérrez and Lorena Gutiérrez
- Abstract
La educación ambiental es imprescindible para lograr un manejo sustentable de los recursos naturales, sobre todo en ecosistemas frágiles como lo es el desierto de Chihuahua. Estrategias para incluir información local ambiental, tanto en el salón de clases como fuera de él son necesarias. Una de estas estrategias, se conoce como “localmente relevante” y está enfocada a la enseñanza de la geografía, flora y fauna locales de manera que el niño se familiarice con los recursos naturales y culturales de su entorno. Un esfuerzo proactivo hacia este fin, es el libro de actividades para niños -Juega y Aprende con Animales del Desierto de Chihuahua- el cual complementa la educación ambiental con información de fauna del desierto por medio de actividades. El libro está disponible en Internet.
- Published
- 2018
43. The Nuclear Pore Complex Is a Key Target of Viral Proteases to Promote Viral Replication
- Author
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Selvin Noé Palacios-Rápalo, José Manuel Reyes-Ruiz, Rosa M. del Angel, Carlos Noe Farfan-Morales, Luis Adrián De Jesús-González, Juan Fidel Osuna-Ramos, Carlos Daniel Cordero-Rivera, and Ana Lorena Gutiérrez-Escolano
- Subjects
0301 basic medicine ,viruses ,Active Transport, Cell Nucleus ,Review ,Biology ,Virus Replication ,Microbiology ,Cell Line ,03 medical and health sciences ,Immune system ,flavivirus ,nuclear pore complex ,Virology ,Humans ,Nuclear pore ,Transcription factor ,Messenger RNA ,030102 biochemistry & molecular biology ,enterovirus ,Viral Proteases ,nucleus ,Translation (biology) ,biology.organism_classification ,QR1-502 ,Cell biology ,Flavivirus ,030104 developmental biology ,Infectious Diseases ,Viral replication ,Cytoplasm ,Viruses ,Nuclear Pore - Abstract
Various viruses alter nuclear pore complex (NPC) integrity to access the nuclear content favoring their replication. Alteration of the nuclear pore complex has been observed not only in viruses that replicate in the nucleus but also in viruses with a cytoplasmic replicative cycle. In this last case, the alteration of the NPC can reduce the transport of transcription factors involved in the immune response or mRNA maturation, or inhibit the transport of mRNA from the nucleus to the cytoplasm, favoring the translation of viral mRNAs or allowing access to nuclear factors necessary for viral replication. In most cases, the alteration of the NPC is mediated by viral proteins, being the viral proteases, one of the most critical groups of viral proteins that regulate these nucleus–cytoplasmic transport changes. This review focuses on the description and discussion of the role of viral proteases in the modification of nucleus–cytoplasmic transport in viruses with cytoplasmic replicative cycles and its repercussions in viral replication.
- Published
- 2021
44. Exploring the Preference for Fatigue-avoidance Goals as a Mediator Between Pain Catastrophizing, Functional Impairment, and Walking Behavior in Women With Fibromyalgia
- Author
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Lilian Velasco, Irene López-Gómez, Lorena Gutiérrez, Carmen Écija, Patricia Catalá, and Cecilia Peñacoba
- Subjects
Cross-Sectional Studies ,Fibromyalgia ,Catastrophization ,Quality of Life ,Humans ,Pain ,Female ,Walking ,Middle Aged ,Goals ,Fatigue - Abstract
Along with the symptoms of pain and fatigue, pain catastrophizing and avoidance behaviors are highly prevalent and associated with functional impairment in fibromyalgia (FM) patients. Although pain and fatigue affect patients' quality of life, research has been exclusively focused on how pain affects physical activity and exercise. The aim of this study was to analyze the role of pain catastrophizing and preference for fatigue-avoidance goals in walking behavior and functional impairment in women with FM.In this cross-sectional study the sample was composed of 76 participants aged 18 years and older (mean age=55.05, SD=7.70). The study evaluated pain catastrophizing, preference for fatigue-avoidance goals, functional impairment, and walking behavior along with sociodemographic variables and clinical data.Pain catastrophizing was associated with preference for fatigue-avoidance goals and this preference was associated with greater functional impairment and less distance walked. Path analysis supported the mediating role of preference for fatigue-avoidance goals in the relationship between pain catastrophizing and walking behavior and between pain catastrophizing and functional impairment. Furthermore, pain catastrophizing predicted greater preference for fatigue-avoidance goals which predicted more problems in functioning and less distance walked.The present study may help clarify the connection between the factors that stop individuals with FM from implementing beneficial behaviors such as walking, and thus, allowing for the design of psychological interventions that seek to maintain physical functioning despite experiencing fatigue.
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- 2021
45. Time out! Pauses during advanced life support in high-fidelity simulation: A cross-sectional study
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Verónica V. Márquez-Hernández, Gabriel Aguilera-Manrique, Alba García-Viola, José Miguel Garrido-Molina, Lorena Gutiérrez-Puertas, Mª Carmen Rodríguez-García, and Vanesa Gutiérrez-Puertas
- Subjects
Time-out ,medicine.medical_specialty ,Cross-sectional study ,Defibrillation ,business.industry ,medicine.medical_treatment ,Electric Countershock ,Emergency Nursing ,Critical Care Nursing ,Positive correlation ,Checklist ,Cardiopulmonary Resuscitation ,Advanced life support ,Heart Arrest ,High Fidelity Simulation Training ,Physical medicine and rehabilitation ,Cross-Sectional Studies ,Life support ,High fidelity simulation ,medicine ,Humans ,business - Abstract
Background Prolonged preshock pauses are associated with negative effects on patient outcomes and survival. A greater understanding of these pauses may help to improve the quality of advanced life support (ALS) and clinical outcomes. Objective The objective of this study was to identify the pauses that occur during ALS situations in high-fidelity simulation scenarios and the frequency and duration of these pauses. Methods One hundred forty-two nursing students participated in this cross-sectional study, involving high-fidelity simulation scenario of cardiorespiratory arrest in a simulated hospital room. Pauses were assessed using an observation checklist. Results Students performed the scenario in an average time of 8.32 (standard deviation = 1.13) minutes. Pauses between chest compressions were longer than recommended (mean = 0.36, standard deviation = 1.14). A strong positive correlation was found between the identification of the arrhythmia and the initiation of countershock (rs = 0.613, p Conclusions Nursing students generally performed ALS within the time limits recommended by resuscitation guidelines. Early identification of shockable rhythms may lead to early nurse-initiated defibrillation. Strategies to speed up the identification of arrhythmias should be put in place to minimise preshock pauses and improve ALS outcomes.
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- 2021
46. Does Treatment Impact on the Quality of Life in Breast Cancer Patients? A Comparative Study Between Treatments
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Elisabeth Berzal Pérez, Lilian Velasco Furlong, Natasha Alcocer Castillejos, Lorena Gutiérrez Hermoso, Paulina Quiroz Friedman, and Sofía Sánchez Román
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Oncology ,medicine.medical_specialty ,Text mining ,Breast cancer ,business.industry ,Internal medicine ,medicine ,business ,medicine.disease - Abstract
BackgroundBreast cancer involves complicated emotional processes. One of the factors that impacts the psychological symptoms and decreases QoL is the side effects of treatment. This study compared the psychological and physical impacts of women with early stages of breast cancer (stages I to III) being treated with chemotherapy versus hormone therapy. This study also intended to identify which symptoms could predict a decrease in quality of life (QoL) and functionality according to the treatment. MethodThis study had a cross-sectional design. The participant sample comprised 129 women with breast cancer (63 receiving chemotherapy and 66 hormone therapy). ResultsSignificant differences were found in groups regarding QoL, functionality, symptomatology (fatigue and nausea), coping strategies, and alexithymia. Furthermore, in patients receiving chemotherapy, both symptomatologies explained the variance in QoL, but only fatigue explained role functioning. For patients receiving hormone therapy, fatigue was the symptom that explained the variance for both QoL and role functioning. ConclusionsThe results confirmed that women undergoing chemotherapy are more physically and psychologically impacted. For both treatments, fatigue was a crucial symptom that affected QoL and role functioning. These results show the need to help women alleviate their psychological symptoms because they can affect their physical condition.
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- 2021
47. Una propuesta metodológica innovadora para analizar el conocimiento tradicional relativo a la biodiversidad vegetal desde una perspectiva de género
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Juana Labrador Moreno, Trinidad Ruiz Téllez, José Blanco Salas, and Lorena Gutiérrez García
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Frente a la desaparición acelerada de los conocimientos tradicionales en la sociedad actual, nace la necesidad de desarrollar estrategias que permitan su conservación. Una herramienta eficaz es la Etnobiología, encargada del estudio de las relaciones entre el medio natural y el ser humano. Esta disciplina suele hacer uso de metodologías que implican investigaciones muy dilatadas en el tiempo. En este trabajo se describe un nuevo método cuantitativo más rápido y eficiente, basado en la recopilación de información mediante talleres y el cálculo del Índice de Significación Cultural (CSI). La eficiencia de dicho método ha sido probada en dos estudios distintos, demostrándose su utilidad para la obtención de información relevante sobre los conocimientos bioculturales desde una perspectiva de género.
- Published
- 2021
48. Enseñanza de la Botánica en un contexto local: una propuesta didáctica basada en el naranjo morisco de Hornachos - [Teaching Botany from a local context: a didcatic proposal based on Hornacho’s Moorish orange tree]
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José Blanco-Salas, Jesús Sánchez-Martín, Isaac Corbacho-Cuello, Lorena Gutiérrez-García, and Trinidad Ruiz-Téllez
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- 2021
49. Worldviews on Evidence-Based Cardiopulmonary Resuscitation Using a Novel Method
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Verónica V. Márquez-Hernández, Gabriel Aguilera-Manrique, Alba García-Viola, Alfredo Alcayde-García, José Miguel Garrido-Molina, and Lorena Gutiérrez-Puertas
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Evidence-based practice ,Computer science ,Health, Toxicology and Mutagenesis ,medicine.medical_treatment ,Therapeutic treatment ,database management ,Public Health, Environmental and Occupational Health ,Scopus ,cardiac arrest ,medicine.disease ,cardiopulmonary resuscitation ,worldwide research ,Article ,Field (computer science) ,Heart Arrest ,Scientific evidence ,medicine ,Humans ,Medicine ,Cardiopulmonary resuscitation ,Medical emergency ,Working group ,network analysis - Abstract
The evaluation of scientific content by researchers, as well as the knowledge networks and working groups of cardiopulmonary resuscitation, can help to improve and expand new scientific evidence in this field. The aim of this study was to identify the global scientific publications on cardiopulmonary resuscitation research using a novel method. The method used was based on obtaining bibliographic data automatically from scientific publications through the use of the Scopus Database API Interface. A total of 17,917 results were obtained, with a total of 60,226 reports and 53,634 authors. Six categories were detected with 38.56% corresponding to cardiac arrest, 21.8% to cardiopulmonary resuscitation, 17.16% to life-support training and education, 12.45% to ethics and decision-making in cardiac arrest, 4.77% to therapeutic treatment, and 3.72% to life-support techniques. Analyzing and identifying the main scientific contributions to this field of study can make it possible to establish collaboration networks and propose new lines of research, as well as to unify criteria for action. Future research should delve into the analyses of the other elements involved in this area.
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- 2021
50. Multiplex PCR method for the detection of human norovirus, Salmonella spp., Shigella spp., and shiga toxin producing Escherichia coli in blackberry, coriander, lettuce and strawberry
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Juan Ramiro Pacheco-Aguilar, Sofía M. Arvizu-Medrano, Cleo Cancio-Lonches, Ana Lorena Gutiérrez-Escolano, Rocío Morales-Rayas, Montserrat H. Iturriaga, and Omar Hernández Hernández
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Salmonella ,ved/biology.organism_classification_rank.species ,Population ,Coriandrum ,Food Contamination ,Biology ,medicine.disease_cause ,Fragaria ,Microbiology ,fluids and secretions ,Vancomycin ,STX2 ,Multiplex polymerase chain reaction ,medicine ,Shigella ,education ,education.field_of_study ,Shiga-Toxigenic Escherichia coli ,ved/biology ,Norovirus ,Pathogenic bacteria ,Lettuce ,Fruit ,Food Microbiology ,Rubus ,Multiplex Polymerase Chain Reaction ,Novobiocin ,Food Science ,Murine norovirus - Abstract
A multiplex PCR method was developed for the simultaneous detection of murine norovirus (MNV-1) as a surrogate for human norovirus (HuNoV) GI and GII, Salmonella spp., Shigella spp., and Shiga toxin producing Escherichia coli (STEC) in fresh produce. The toxicity of the glycine buffer on bacterial pathogens viability was evaluated. The growth of each of the three pathogens (previously stressed) was evaluated at 35 and 41.5 °C in modified buffered peptone water (mBPW) and trypticase soy broth (TSB), supplemented with vancomycin, novobiocin and brilliant green at two concentration levels. The selected conditions for simultaneous enrichment were: 41.5 °C/mBPW/supplemented with 8 ppm vancomycin, 0.6 ppm novobiocin and 0.2 ppm brilliant green. The pathogens and aerobic plate count (APC) growth was evaluated in the enrichment of lettuce, coriander, strawberry and blackberry under the best enrichment conditions. Starting from 1 to 10 CFU/mL, Salmonella reached from 7.63 to 8.91, Shigella 6.81 to 7.76 and STEC 7.43 to 9.27 log CFU/mL. The population reached for the APC was 5.11–6.56 log CFU/mL. Simultaneous detection by PCR was done using designed primers targeting invA, ipaH, stx1 and stx2 genes, and MNV-1. The detection sensitivity was 10–100 PFU for the MNV-1 and 1–10 CFU for each pathogenic bacteria. This protocol takes 6 h for MNV-1 and 24 h for Salmonella spp., Shigella spp., and STEC detection from the same food portion. In total, 200 samples were analyzed from retail markets from Queretaro, Mexico. Two strawberry samples were positive for HuNoV GI and one lettuce sample was positive for STEC. In conclusion, the method developed in this study is capable of detecting HuNoV GI and GII, Salmonella spp., Shigella spp and STEC from the same fresh produce sample.
- Published
- 2022
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