1. Comparison of HER2 status by fluorescence in situ hybridisation and immunohistochemistry in gastric cancer
- Author
-
Zhou Ming, Ming Chen, Ai Biao, Liu Xue Zheng, and Yan Li
- Subjects
Original Paper ,Pathology ,medicine.medical_specialty ,fluorescence in situ hybridisation ,Her2 expression ,business.industry ,gastric cancer ,Concordance ,Cancer ,human epidermal growth factor receptor-2 ,medicine.disease ,Fluorescence ,Oncology ,In situ hybridisation ,immunohistochemistry ,HER2 Gene Amplification ,Immunohistochemistry ,Medicine ,Radiology, Nuclear Medicine and imaging ,Clinical significance ,skin and connective tissue diseases ,business - Abstract
Aim of the study To investigate the differences and relevance, and to evaluate the clinical significance of fluorescence in situ hybridisation and immunohistochemistry (IHC) in detecting HER2 in gastric cancer tissues. Material and methods The expression of HER2 protein and the amplification of the HER2 gene in 118 gastric cancer tissues were detected by immunohistochemistry and fluorescence in situ hybridisation, respectively. Results Using IHC, we found that in 40 cases (33.9%) the HER2 expression was at level 0, in 33 cases (28.0%) the HER2 expression was at level 1+, in 16 cases (14%) the HER2 expression was at level 2+, and in 29 cases (25.6%) the HER2 expression was at level 3+, respectively. Using the FISH test, 38 of 118 cases (32.2%) were judged as positive results. The concordance rate between the results of IHC and FISH in all cases was 85.6%. The concordance rate of IHC and FISH was high in cases of HER2 expression at level 0, 1+ and 3+ according to IHC, but low in cases of 2+ according to IHC (43.8%). Conclusions Immunohistochemistry cannot predict HER2 gene amplification accurately. The FISH test should be executed in IHC 2+ cases.
- Published
- 2014