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The main aim was to develop a standardized survey instrument for measuring job stress and well-being in hospital settings. The actual study group consisted of 349 workers from medical bed wards, first aid unit wards and bed wards for gynecology and obstetrics in a middle-sized hospital in the Helsinki region. Based on the factor analysis of separate questions, the following content areas were chosen for the job stressor scales: haste at work, problems in interpersonal relations at work, problems in occupational collaboration with others, too much responsibility, safety and health risks, lack of appreciation, troublesome patients, and lack of equipment and resources. Content areas for well-being scales and items were general job satisfaction, strain symptoms, perceived mental and physical work load. The reference values of the questionnaire and reliabilities for the scales were calculated. The application and further development of the questionnaire was discussed.