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Additional file 1: Supplemental material.

Additional file 1.

Additional file 1: Figure S1. Study areas of Taizhou City in China. The map was created by ArcGIS 10.2 (Software, ESRI Inc., Redlands, CA, USA). The base layer of the map of Zhejiang Province was supported from National Earth System Science Data Center, National Science & Technology Infrastructure of China (http://www.geodata.cn). Figure S2. With the median as the reference, the lag effect between Avetemp, Maxtemp, Mintemp, RH and WTP and HFRS infection. Abbreviations: Avetemp, average temperature; CI, confidence interval; df, degree of freedom; DLNM, distributed lag non-linear model; GAM, generalized additive model; HFRS, Hemorrhagic fever with renal syndrome; Maxtemp, maximum temperature; Mintemp, minimum temperature; RH,relative humidity;WTP,weekly total precipitation; RR, relative risk.

Obstract: To characterize and analyze risky sexual networks and genetic scales to potential HIV transmission for HIV seroconcordant couples in Taizhou municipality of Zhejiang Province.HIV seroconcordant positive couples were invited as index cases to participate in an egocentric survey on HIV related risky behavior and behavioral network prior to HIV diagnosis during 2008-2011. Within-couple HIV transmission pairs were determined by the combination of both behavioral and phylogenetic analysis.Totally 27 HIV seroconcordant couples were enrolled in this study. Male spouses were more likely to report having two or more sexual partners in the past years prior to HIV diagnosis than female spouses (88.9% vs. 37.0%). Among 27 couples, 20 couples including 17 couples by male but not female spouses, 3 couples by female but not male spouses reported having two or more sexual partners (i.e., multiple sexual partners) prior to HIV diagnosis; and 7 couples by both spouses reported having multiple sexual partners. Twenty four of 27 sexual networks were determined to be HIV transmission pairs (20) or potential transmission pairs (4), 3 couples were subtyped with discordant HIV subtypes or large genetic distance and thus had different sources of HIV transmissions. In addition, among 27 concordant couples, HIV drug resistance (HIVDR) or primary HIVDR existed in 6 ART-naïve participants in 4 networks; among them, 2 networks were determined to be potential HIVDR transmission couple pairs.The HIV strains isolated in HIV infected spouses characterized with diversity and CRF01_AE was the main strain subtype. One of the spouses with risky behavior infected HIV was the main route of transmission to other spouses through unprotected sexual contacts. HIVDR was isolated from some HIV infected individuals, suggesting the risk for HIVDR transmission in married couples. The results provide enhanced evidence for urgent development of tailored prevention strategies, such as couple-based HIV counseling and testing services to reduce HIV secondary transmission.

The factors are analyzed in this paper that influence the guide bearing load of a large vertical pump from structures; errors of design, manufacture, construction and installation of pump unit and assembly. The error randomicity results in the bearing load randomicity. The probability load theory of the guide bearing is established for the first time. Solving methods of the model and characteristics of the bearing probability load are studied. The guiding bearing design load is recommended. The results mean much to rational design, load decrease and increase of service life of the guide bearing.

The influences of angle of flare,section figure and centerline of strike of discharge passage of a large pump on its hydraulic loss are studied.The concept of equivalent angle of flare is advanced.For the purpose of minimizing discharge pas- sage total hydraulic loss,which includes frictional hydraulic loss,diffuse hydraulic loss and exit hydraulic loss,the angle of flare and section figure of the passage are optimized,the opti- mizing principles of the passage centerline of strike are put forward.The research results indicate that the passage has the optimum angle of flare which makes its hydraulic loss the least for certain positions of inlet section and exit section.Tests prove that the actual angle of flare from which separation fluids just occur is smaller than the optimum angle of flare obtained by theoretical calculation because of the influences of discharge curved pipe.The gradual changing optimum angle of flare could make the hydraulic loss even smaller than the constant optimum angle of flare.The optimum passage section figure of rectangle is square,and the rectangle has 4 optimum comer circular arcs which make the hydraulic loss of friction loss of per unit length passage the least.The results are significant for decreasing hy- draulic loss of discharge passage,pump assembly optimum design and increasing pump assembly efficiency.