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The paper investigates the ability to retrieve the true soil moisture profile by assimilating near-surface soil moisture into a soil moisture model with an ensemble Kaiman filter (EnKF) assimilation scheme, including the effect of ensemble size, update interval and nonlinearities in the profile retrieval, the required time for full retrieval of the soil moisture profiles, and the possible influence of the depth of the soil moisture observation. These questions are addressed by a desktop study using synthetic data. The “true” soil moisture profiles are generated from the soil moisture model under the boundary condition of 0.5 cm d−1 evaporation. To test the assimilation schemes, the model is initialized with a poor initial guess of the soil moisture profile, and different ensemble sizes are tested showing that an ensemble of 40 members is enough to represent the covariance of the model forecasts. Also compared are the results with those from the direct insertion assimilation scheme, showing that the EnKF is superior to the direct insertion assimilation scheme, for hourly observations, with retrieval of the soil moisture profile being achieved in 16 h as compared to 12 days or more. For daily observations, the true soil moisture profile is achieved in about 15 days with the EnKF, but it is impossible to approximate the true moisture within 18 days by using direct insertion. It is also found that observation depth does not have a significant effect on profile retrieval time for the EnKF. The nonlinearities have some negative influence on the optimal estimates of soil moisture profile but not very seriously.