Search

Yong-Guang Wei, Cheng-Kun Yang, Zhong-Liu Wei, Xi-Wen Liao, Yong-Fei He, Xin Zhou, Hua-Sheng Huang, Chen-Lu Lan, Chuang-Ye Han, Tao Peng Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, People’s Republic of ChinaCorrespondence: Tao PengDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University, Shuang Yong Road 6, Nanning, Guangxi Zhuang Autonomous Region, People’s Republic of ChinaTel +86-771-5356528Fax +86-771-5350031Email pengtaogmu@163.comBackground: The protein high-mobility group AT-hook 1 (HMGA1) has been demonstrated that modulated cellular proliferation, invasion, and apoptosis with a poor prognosis in miscellaneous carcinomas. However, the mechanism of circumstantial carcinogenesis and association with the immune microenvironment of HMGA1 in hepatocellular carcinoma (HCC) had not been extensively explored.Methods: The gene expression, clinicopathological correlation, and prognosis analysis were performed in the data obtained from TCGA. The results were further validated by ICGC and GEO database and external validation cohort from Guangxi. The HMGA1 protein expression was further examined in the HPA database. Biological function analyses were conducted by GSEA, STRING database, and Coexpedia online tool. Using TIMER and CIBERSORT method, the relationship between immune infiltrate and HMGA1 was investigated.Results: In HCC, HMGA1 had much higher transcriptional and proteomic expression than in corresponding paraneoplastic tissue. Patients with high HMGA1 expression had a poor prognosis and unpromising clinicopathological features. High HMGA1 expression was closely related to the cell cycle, tumorigenesis, substance metabolism, and immune processes by regulating complex signaling pathways. Notably, HMGA1 may be associated with TP53 mutational carcinogenesis. Moreover, increased HMGA1 expression may lead to an increase in immune infiltration and a decrease in tumor purity in HCC. CIBERSORT analysis elucidated that the amount of B cell naive, B cell memory, T cells gamma delta, macrophages M2, and mast cell resting decreased when HMGA1 expression was high, whereas T cells follicular helper, macrophages M0, and Dendritic cells resting increased.Conclusion: In conclusions, HMGA1 is a potent prognostic biomarker and a sign of immune infiltration in HCC, which may be a potential immunotherapy target for HCC.Keywords: hepatocellular carcinoma, HMGA1, prognostic signature, bioinformatics, immune filtration

A biomimetic vascular scaffold with excellent mechanical properties and biocompatibility is urgently needed, and designing such a scaffold is a challenge. The majority of vascular scaffolds have satisfactory biocompatibility, but poor mechanical properties. Herein, biodegradable poly(l-lactide-co-caprolactone) (PLCL) with biomimetic mechanical properties was used to prepare small-diameter (

Additional file 10. Table S1: Clinical data of 159 HCC patients in the CHCC cohort.

Spatial covariance matrix (SCM) is essential in many applications of multi-antenna systems such as massive multiple-input multiple-output (MIMO). For massive MIMO operating at millimeter-wave bands, hybrid analog-digital structure has been adopted to reduce the cost of radio frequency (RF) chains. In this situation, signals received at the antennas are unavailable to the digital receiver, and as a consequence, traditional sample average approach cannot be used for SCM reconstruction in hybrid massive MIMO. To address this issue, beam sweeping algorithm (BSA), which can reconstruct SCM effectively in hybrid massive MIMO, has been proposed in our previous work. In this paper, a truncated BSA is further proposed for SCM reconstruction by taking into account the patterns of antenna elements in the array. Due to the directive antenna pattern, sweeping results corresponding to predetermined direction-of-angles (DOA) far from the normal direction are small and thus can be replaced by predetermined constants. At the cost of negligible performance reduction, SCM can be reconstructed efficiently by sweeping only the predetermined DOAs that are close to the normal direction. In this way, BSA can be conducted much faster than its traditional counterpart. Insightful analysis will be also included to show the impact of truncation on the performance.

Additional file 11.Table S2. Joint effects analysis of CDK1 and CDK4 expression in CHCC cohort.

Additional file 2: Table 2. Basic characteristics of pancreatic ductal adenocarcinoma patients in The Cancer Genome Atlas database.

Additional file 3: Table 3. Basic characteristics of PDAC patients in Gene Expression Omnibus database.

Pollution by atmospheric particulate matter (PM) is a serious threat to human health, and traditional window screens are unable to intercept fine particles. Therefore, it is urgent to develop anti-haze window screens to effectively protect the public. We prepared a polyurethane/silicon nitride (PU/Si3N4) electret nanofiber membrane with a minimum diameter (350 nm) and narrower diameter distribution than those of PU-Boehmite, PU-SiO2, and PU-TiO2 membranes by electrospinning. The PU-Si3N4 fiber membrane has good mechanical properties with stress of 18.05 MPa and strain of 170%. Moreover, PU/Si3N4 exhibits a maximum current peak (2.63 pA) at 90 °C. Importantly, PU/Si3N4 membranes applied to window screens still maintain high filtration efficiency (79.36%) and low resistance (25 Pa) to air flow at a low areal density of 1.22 g/m2. Furthermore, they show good light transmittance (40%) and air permeability (46.42 mm s−1). Therefore, PU/Si3N4 electret nanofiber window screens have broad application prospects.

Waterproof-breathable (WB) materials with outstanding waterproofness, breathability, and mechanical performance are critical in diverse consumer applications. Electrospun nanofibrous membranes with thin fiber diameters, small pore sizes, and high porosity have attracted significant attention in the WB fabric field. Hot-press treatment technology can induce the formation of inter-fiber fusion structures and hence improve the waterproofness and mechanical performance. By combining electrospinning and hot-press treatment technology, polyurethane/fluorinated polyurethane/thermoplastic polyurethane/alkylsilane-functionalized graphene (PU/FPU/TPU/FG) nanofiber WB fabric was fabricated. Subsequently, the morphologies, porous structure, hydrostatic pressure, water vapor transmission rate (WVTR), and stress&ndash, strain behavior of the nanofiber WB fabric were systematically investigated. The introduction of the hydrophobic FG sheet structure and the formation of the inter-fiber fusion structure greatly improved not only the waterproofness but also the mechanical performance of the nanofiber WB fabric. The optimized PU/FPU/TPU-50/FG-1.5 WB fabric exhibited an excellent comprehensive performance: a high hydrostatic pressure of 80.4 kPa, a modest WVTR of 7.6 kg m&minus, 2 d&minus, 1, and a robust tensile stress of 127.59 MPa, which could be used to achieve various applications. This work not only highlights the preparation of materials, but also provides a high-performance nanofiber WB fabric with huge potential application prospects in various fields.

When China is vigorously urging the development of western area and the construction of new village, how to improve living standards of the famers at the same time to maximize the energy saving and the reduction of building materials using is one of the important problems that need to be solved. Based on the survey and research of the rural house in the Guanzhong region of north china, paper explores and analyzes the orientation, architectural style, layout, natural ventilation etc. Then arrive at the general applicability of ecological and energy-saving rural house in the Guanzhong region under the concept of ecological design. In the wake of the help of generally applicable design program, can provide some reference for the current rural house construction, and promote the integration of urban and rural areas.

Cave dwelling is a traditional architectural form in the northern part of Shaanxi province. It is the product of local traditional culture and humanistic spirit, and corresponds with the requirements of energy-saving and sustainable development. This paper takes the traditional cave dwelling in the northern part of Shaanxi as the research object, and uses two kinds of methods: the analysis of the literature data and on-the-spot investigation to put forwards the traditional ventilation design strategies of cave dwelling construction in the northern part of Shaanxi province, which aims to explore the new methods of reforming traditional cave dwelling construction.

Nowadays, with the deteriorating environment, people are increasingly concerned about the ecological environment and sustainable development. More and more architects are committed to sustainable building design and research. In China, designers, professors and students in the architecture realm are encouraged to get involved into sustainable architecture design practically/theoretically. With this background this paper presents a case study of a sustainable architectural design called “Painter’s House”, which concentrates on the openness and ecology of architecture. It contains concepts and ideas of functional diversity, integrated sustainable architectural design, as well as “eco-box”, hoping to induce more profound discussion in the sustainable architectural design area.

In recent decades, sustainable buildings have been increasingly concerned, architects, teachers and college students are involved into a number of theoretical and practical exploration. A variety of sustainable building design strategies and cases spring up. Taking Houji Jiaojia garden agricultural exhibition pavilion design as an example, on the basis of exhausting analysis of the local climate, geographical conditions, it focuses particularly on the use of local ecological straw materials in the building to achieve the sustainability of the project, which can provide some reference for the current and future sustainable architectural design.

To establish a rat model of chronic pancreatitis, and to prove the activation of sonic hedgehog (SHH) signaling pathways in chronic pancreatitis.This study was conducted between January and July 2008 in the Department of General Surgery, Wuhan General Hospital, Guangzhou Military Command, Wuhan, China. Thirty Wistar rats were randomly divided into 3 groups: control group (A), experimental control group (B), and model group (C) (10 rats in each group). Trinitrobenzene sulfonic acid was infused into the pancreatic duct to induce chronic pancreatitis in the model group rats. In the experimental control group, we opened the abdominal cavity and infused with 0.9% sodium chloride solution. Serum levels of bilirubin and amylase were determined by radioimmunoassay. Histopathological alterations were studied using the optical microscopy. Expression of patched-1 (PTCH-1), smoothened (SMO), and SHH were detected by immunohistochemistry.Compared with the control group (A), the serum bilirubin and amylase in the model group increased significantly after 7 days of treatment, and fibrotic proliferation of pancreatic tissues were found after 35 days; the expression of PTCH-1, SMO, and SHH in the pancreatic tissue increased significantly in the model group.Trinitrobeneze sulfonic acid can induce chronic pancreatitis in rat. The SHH signaling pathway is activated in rats with chronic pancreatitis.

A method has been developed for determining microamounts of molybdenum(VI) in aqueous solution by means of the Mo-o-nitrophenylfluorone—cetyltrimethylammonium bromide system, in which micellar solubilization is applied. A red complex is formed in 0.2–0.6M hydrochloric acid medium. The sensitivity of the method is high, and the apparent molar absorptivity is 1.55 × 105 l.mole−1. cm−1. The absorption peak of the complex appears at 530 nm. The colour of the complex develops quickly and is stable for more than 24 hr. The composition of the complex is Mo: o-NPF = 1:1, and the system obeys Beer's law in the range 0–10 μg of Mo per 25 ml. The method has been used for the rapid determination of molybdenum in alloy steels with satisfactory results.