1. Development, expansion, and in vivo monitoring of human NK cells from human embryonic stem cells (hESCs) and and induced pluripotent stem cells (iPSCs)
- Author
-
Bock, Allison M, Knorr, David, and Kaufman, Dan S
- Subjects
Pluripotent Stem Cells ,Physiology ,Cytological Techniques ,Induced Pluripotent Stem Cells ,Adoptive ,FACS ,Biomedical Engineering ,iPSCs ,Bioengineering ,ES Cells ,NK cells ,HSC ,Regenerative Medicine ,Biochemistry ,stem cells ,Killer Cells ,Humans ,Psychology ,Stem Cell Research - Embryonic - Human ,Luciferases ,Issue 74 ,Molecular Biology ,Firefly ,Embryonic Stem Cells ,Transplantation ,cell culture ,Stem Cell Research - Induced Pluripotent Stem Cell - Human ,Stem Cell Research - Induced Pluripotent Stem Cell ,Stem Cell Biology ,Cell Differentiation ,turboFP650 ,Hematology ,differentiation ,Stem Cell Research ,Hematopoietic Stem Cells ,fluorescent imaging ,Killer Cells, Natural ,Cellular Biology ,ESCs ,Natural ,Medicine ,Cognitive Sciences ,Generic health relevance ,Immunotherapy ,Biochemistry and Cell Biology ,Anatomy ,in vivo imaging ,Biotechnology - Abstract
We present a method for deriving natural killer (NK) cells from undifferentiated hESCs and iPSCs using a feeder-free approach. This method gives rise to high levels of NK cells after 4 weeks culture and can undergo further 2-log expansion with artificial antigen presenting cells. hESC- and iPSC-derived NK cells developed in this system have a mature phenotype and function. The production of large numbers of genetically modifiable NK cells is applicable for both basic mechanistic as well as anti-tumor studies. Expression of firefly luciferase in hESC-derived NK cells allows a non-invasive approach to follow NK cell engraftment, distribution, and function. We also describe a dual-imaging scheme that allows separate monitoring of two different cell populations to more distinctly characterize their interactions in vivo. This method of derivation, expansion, and dual in vivo imaging provides a reliable approach for producing NK cells and their evaluation which is necessary to improve current NK cell adoptive therapies.
- Published
- 2013