1. Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5
- Author
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Paulsen, IT, Press, CM, Ravel, J, Kobayashi, DY, Myers, GSA, Mavrodi, DV, DeBoy, RT, Seshadri, R, Ren, Q, Madupu, R, Dodson, RJ, Durkin, AS, Brinkac, LM, Daugherty, SC, Sullivan, SA, Rosovitz, MJ, Gwinn, ML, Zhou, L, Schneider, DJ, Cartinhour, SW, Nelson, WC, Weidman, J, Watkins, K, Tran, K, Khouri, H, Pierson, EA, Pierson, LS, Thomashow, LS, and Loper, JE
- Subjects
Base Sequence ,Genes, Bacterial ,Multigene Family ,Molecular Sequence Data ,Siderophores ,Biological Transport ,Sequence Analysis, DNA ,Plants ,Pseudomonas fluorescens ,Genome, Bacterial - Abstract
Pseudomonas fluorescens Pf-5 is a plant commensal bacterium that inhabits the rhizosphere and produces secondary metabolites that suppress soilborne plant pathogens. The complete sequence of the 7.1-Mb Pf-5 genome was determined. We analyzed repeat sequences to identify genomic islands that, together with other approaches, suggested P. fluorescens Pf-5's recent lateral acquisitions include six secondary metabolite gene clusters, seven phage regions and a mobile genomic island. We identified various features that contribute to its commensal lifestyle on plants, including broad catabolic and transport capabilities for utilizing plant-derived compounds, the apparent ability to use a diversity of iron siderophores, detoxification systems to protect from oxidative stress, and the lack of a type III secretion system and toxins found in related pathogens. In addition to six known secondary metabolites produced by P. fluorescens Pf-5, three novel secondary metabolite biosynthesis gene clusters were also identified that may contribute to the biocontrol properties of P. fluorescens Pf-5.
- Published
- 2005