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The development of natural-based wound dressings is of great interest in the field of skin tissue engineering. Herein, different bioactive molecules such as gelatin (GEL), chitosan (CH) and mucilage (MU) were used to prepare a wound dressing. The physico-chemical and biological characterizations occurring after γ-irradiation were investigated. Results showed that Electron Paramagnetic Resonance (EPR) spectroscopy of un-irradiated GEL-CH-MU biomaterial showed two paramagnetic centers which correspond to g = 1.89 and g = 2.033. A generated new active center appeared at g = 2.003 at 25 kGy due to the interactions of gamma rays with the polymer chain creating signals at the absorbing functional groups. X-ray diffraction (XRD) spectra preserved the semi-crystalline structures between a range of 2θ (5° and 45°). Fourier Transform Infrared spectroscopy (FTIR) revealed that the initiation of cross linking phenomena. Moreover, γ-rays significantly increased antioxidant activity (9.1 ± 0.07%, p

Bone substitutes are biomaterials applied in surgical therapy to repair many cases of a damaged bone. More than two million bone surgeries using biomaterials are realised each year worldwide. In this line of research, the researchers of this project study developed a bioresorbable synthetic bone substitute designated by (β-TCP/DCPD)-PHBV which is a mixture of the two materials: β-TCP/DCPD and PHBV in a 60/40 weight fraction. This research study attempts to categorize the (β-TCP/DCPD)-PHBV and evaluate its ability on triggering bone regeneration by making bone implants on 12 New Zealand white male rabbits. The RESEARCHERS performed multi-scale studies namely; a uniaxial compression test, an examination by scanning electron microscopy, and analysis with infrared spectroscopy in order to get the characteristics. The neoformed bone is examined by SEM coupled with energy dispersive spectroscopy (EDS). In addition to the salient mechanical benefits; attenuation of the modulus of elasticity and limitation of brittleness, the biomaterial has also proven to be effective in giving rise to new bone tissues having a suitable mineral composition.

The performance therapy of chitosan (CH)-doped bioactive glass (BG) has been evaluated in vitro and in vivo. In vitro, the effect of CH-BG was assessed on human Saos-2 osteoblast cells. In vivo, Wistar rats were ovariectomized (OVX) and CH, BG and CH-BG were implanted in bone tissue. After 3 days of CH-BG contact, cell viability of Saos-2 osteoblast increased by 16.4% as compared to the control group. The runt-related transcription factor 2 (RUNX2/Cbfa1) and osteocalcin (OC) gene expressions were significantly increased with 600 and 300%, respectively, in contact of CH-BG as compared with CH. In vivo, the apoptotic index in the OVX-CH-BG group was decreased by 80%. A mechanical hardness test showed a significant bone strength improvement after CH-BG implantation (40%). The CH-BG composite may therefore prove clinically useful as a bioactive bone substitute.