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Aging is a process of progressive change. To develop biological models of aging, longitudinal datasets with high temporal resolution are needed. Here we report a multi-omics longitudinal dataset for cultured primary human fibroblasts measured across their replicative lifespans. Fibroblasts were sourced from both healthy donors (n = 6) and individuals with lifespan-shortening mitochondrial disease (n = 3). The dataset includes cytological, bioenergetic, DNA methylation, gene expression, secreted proteins, mitochondrial DNA copy number and mutations, cell-free DNA, telomere length, and whole-genome sequencing data. This dataset enables the bridging of mechanistic processes of aging as outlined by the “hallmarks of aging”, with the descriptive characterization of aging such as epigenetic age clocks. Here we focus on bridging the gap for the hallmark mitochondrial metabolism. Our dataset includes measurement of healthy cells, and cells subjected to over a dozen experimental manipulations targeting oxidative phosphorylation (OxPhos), glycolysis, and glucocorticoid signaling, among others. These experiments provide opportunities to test how cellular energetics affect the biology of cellular aging. All data are publicly available at our webtool: https://columbia-picard.shinyapps.io/shinyapp-Lifespan_Study/

Additional file 1: Figure S1. Linear Regression Analysis of 4-Week ECHO Modalities Compared to CMR. Linear regression with correlation coefficients evaluating volumetric measurements of 4D-US, 2D-US, and M-Mode at 4 weeks to CMR at 4 weeks.

Additional file 2: Figure S2. Transgenic Mouse Lines have No Differences in EDV, ESV, or EF. α-MHC-Cre (+) x Flox-TFAM and α-MHC-Cre (−) x Flox-TFAM mice were compared at baseline and Week 4 following CAL by 4D-US, 2D-US, and M-mode and demonstrate no significant changes by any modality when comparing EDV, ESV, and EF.

Additional file 4: Table S2. Spearman Correlation Values and p-Values Between Modalities for Assessing Scar Size. Histologic Sections, WMSI from 4D-US, Hyperintense Tissue on CMR, Longitudinal Strain, Long Axis Radial Strain, Long-Axis LV Dyssynchrony, Short Axis Radial Strain, and Short Axis Circumferential Strain are compared to each other modality

The goal of this thesis is to examine the theme of community in two translated works, Paradise in the Sea of Sorrow: Our Minamata Disease and Lake of Heaven, by Ishimure Michiko. I analyze how Ishimure defines a community, and I also look at the tension between insiders of the community with outsiders. Next, I look at Ishimure’s use of genre in Paradise in the Sea of Sorrow; she blends autofiction, autobiography, and illness narratives to give different perspectives to reflect on the Minamata disease health crisis. Through this analysis, I also look at the shift in Ishimure’s tone toward outsiders, moving from anger to reconciliation between the texts in question. Lastly, I comment on the change in Ishimure’s public image and discuss a story where she is the outsider experiencing Tokyo.

The harbor town of Jaffa (Tel Yafo) was vital for the medieval Crusader States, functioning as a place where reinforcements, pilgrims, and communications entered the Latin East. An assemblage of five ship nails from Jaffa that were removed for reuse in the 13th century AD are examined and shown to be informative for understanding Crusader iron production, economic sustainability in the Crusader States, and the connections between northern European and Mediterranean ship construction traditions. Archaeometallurgical analyses of these ship nails demonstrate the first metallographically documented examples of Crusader steel recovered from archaeological contexts, as well as rare evidence of uncorroded Crusader alloys (nonnumismatic). The analysis also provides likely evidence for the use of iron hardware from the northern European tradition in the Crusader-period Levant.

Recent wireless testbed implementations have proven that full-duplex communication is in fact possible and can outperform half-duplex systems. Many of these implementations modify existing half-duplex systems to operate in full-duplex. To realize the full potential of full-duplex, radios need to be designed with self-interference in mind. In our work, we use a novel patch antenna prototype in an experimental setup to characterize the self-interference channel between transmit and receive radios. We derive an equivalent analytical baseband model and propose analog baseband cancellation techniques to complement the RF cancellation provided by the patch antenna prototype. Our results show that a wide bandwidth, moderate isolation scheme achieves up to 2.4 bps/Hz higher achievable rate than a narrow bandwidth, high isolation scheme. Furthermore, the analog baseband cancellation yields a 10-10,000 improvement in BER over RF only cancellation., Comment: 22 pages, 12 figures, submitted to IEEE JSAC special issue on Full-Duplex

Additional file 3: Table S1. Comparison of ESV, EDV, EF, Scar Size, and survival between transgenic groups.

Additional file 3: Table S1. Comparison of ESV, EDV, EF, Scar Size, and survival between transgenic groups.