1. Uridine as a non-toxic actinometer for UV-C treatment: influence of temperature and concentration
- Author
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Jaayke L. Fiege, Benedikt Hirt, Volker Gräf, Stefan Nöbel, Dierk Martin, Jan Fritsche, Katrin Schrader, and Mario Stahl
- Subjects
Multidisciplinary - Abstract
UV-C treatment is an effective method to inactivate microorganisms and therefore gets increasingly more attention in food industry, especially for liquid products. To test and monitor different UV-C reactor designs, a photochemical actinometer is required that gives reliable UV-C dose values and is non-toxic allowing frequent control of the production chain. Here, a variable concentrated aqueous uridine solution is tested as a photochemical actinometer. Uridine reacts at 262 nm by photohydration to a single photoproduct not absorbing any light. A concentration dependent quantum yield (Ф) was quantified in the range of 0.2-3.0 mM uridine. Results show that uridine is as accurate as the commonly accepted iodide/iodate actinometry, but not as precise. Especially at higher concentrations a higher number of measurements becomes necessary. Further, a temperature correction is presented for 10 °C
- Published
- 2022