1. Carbon Dots Enable Efficient Delivery of Functional DNA in Plants
- Author
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Bo Wang, Wang Yun, Yurong Ma, Mingwang Shao, Huibo Wang, Huang Hui, Kang Zhenhui, Fang Lu, Hengxiu Yu, Changhong Liu, Huang Jian, Xiao Wang, Liu Yang, Mengling Zhang, and Xiaodong Zhao
- Subjects
fungi ,Biochemistry (medical) ,Biomedical Engineering ,chemistry.chemical_element ,Gene transfer ,General Chemistry ,Computational biology ,Biology ,Gene gun ,Biomaterials ,chemistry.chemical_compound ,chemistry ,Gene expression ,Carbon ,DNA - Abstract
Genetic engineering is an important technology in plant sciences. The current delivery techniques, such as Agrobacterium-mediated gene transfer and gene gun instant gene expression system, always need a very complicated process or have host-range limitations due to the inhibition of the cell wall structure. Here, we demonstrate that a nanocomposite (CDP) prepared by combining polyethyleneimine (PEI) with carbon dot (CD) was confirmed as a good vector for gene transfection in both animal and plant cells, especially in intact plants. Efficient DNA delivery and strong gene expression without DNA integration are accomplished in rice (
- Published
- 2022