1. Targeting an EGFR Water Network with 4‐Anilinoquin(az)oline Inhibitors for Chordoma
- Author
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Graham J. Tizzard, Tuomo Laitinen, Chad Torrice, William J. Zuercher, Kaitlyn A. Maffuid, Daniel J. Crona, and Christopher R. M. Asquith
- Subjects
Models, Molecular ,Lung Neoplasms ,non-small cell lung cancer (NSCLC) ,Antineoplastic Agents ,Apoptosis ,01 natural sciences ,Biochemistry ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,Drug Discovery ,Chordoma ,medicine ,Humans ,Molecular Targeted Therapy ,Epidermal growth factor receptor ,General Pharmacology, Toxicology and Pharmaceutics ,Protein Kinase Inhibitors ,IC50 ,EGFR inhibitors ,Pharmacology ,Aniline Compounds ,biology ,010405 organic chemistry ,Kinase ,Chemistry ,Cellular Assay ,Organic Chemistry ,Active site ,medicine.disease ,0104 chemical sciences ,ErbB Receptors ,010404 medicinal & biomolecular chemistry ,Cell culture ,Quinazolines ,Cancer research ,biology.protein ,Molecular Medicine ,Drug Screening Assays, Antitumor ,Protein Binding - Abstract
Quinoline- and quinazoline-based kinase inhibitors of the epidermal growth factor receptor (EGFR) have been used to target non-small cell lung cancer (NSCLC) and chordomas with varying amounts of success. We designed and prepared compounds to probe several key structural features including an interaction with Asp855 within the EGFR DGF motif and interactions with the active site water network. EGFR target engagement was then evaluated in a cellular assay, with the inhibitors then profiled in representative cellular models of NSCLC and chordomas. In addition, structure–activity relationship insight into EGFR inhibitor design with potent dimethoxyquin(az)olines identified compounds 1 [N-(3-ethynylphenyl)-6,7-dimethoxyquinolin-4-amine], 4 [N-(3-ethynylphenyl)-6,7-dimethoxyquinazolin-4-amine], and 7 [4-((3-ethynylphenyl)amino)-6,7-dimethoxyquinoline-3-carbonitrile]. We also identified 6,7-dimethoxy-N-(4-((4-methylbenzyl)oxy)phenyl)quinolin-4-amine (compound 18), which is the most potent inhibitor (IC50=310 nm) of the UCH-2 chordoma cell line to date.
- Published
- 2019