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The first complete chloroplast genome (cpDNA) sequence of Kadsura ananosma was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 145,903 bp in length, contains a large single-copy region (LSC) of 94,757 bp and a small single-copy region (SSC) of 18,042 bp, which were separated by a pair of inverted repeats (IR) regions of 16,552 bp. The genome contains 125 genes, including 82 protein-coding genes, 8 ribosomal RNA genes, and 35 transfer RNA genes. Further phylogenomic analysis showed that K. ananosma and Kadsura coccinea clustered in a clade in Schisandraceae family.

Five 3,4-seco-cycloartane triterpenoids were isolated from the stems of Kadsura ananosma, two of which had rearranged 5/6 consecutive carbocycle rings C/D (trivially named ananosins A (1) and B (2)), one had a migrated CH3-18 (named ananosins C (3)), and two were analogs, ananosins D (4) and E (5). Their structures were characterized by comprehensive spectroscopic analysis, especially using 2D NMR spectra. A biogenetic pathway to 1 was proposed. These 5 compounds, together with 5 known analogs isolated from the same origin, were evaluated for their cytotoxicity against HL-60, SMMC-7721, A-549, PANC-1, and SK-BR-3 human cancer cells, but were inactive.

Fourteen new dibenzocyclooctadiene lignans, ananonins A-N (1-14), together with five known compounds, were isolated from the seeds of Kadsura ananosma. The structures and absolute configurations of 1-14 were established using a combination of MS, NMR, and CD techniques. The biological activity of these compounds was evaluated, and ananonin M (13) showed moderate neuroprotective effects in an in vitro assay. (C) 2011 Elsevier Ltd. All rights reserved.

Fourteen new dibenzocyclooctadiene lignans, ananolignans A-N (1-14), together with five known compounds, were isolated from the seeds of Kadsura ana iosma. The structures and absolute configurations of 1-14 were established using a combination of spectroscopic methods including 1D- and 2D-NMR and CD techniques. The biological activity of the isolated lignans was evaluated, and ananolignan F (6) and ananolignan L (12) showed significant neuroprotective effects in an in vitro assay.

An extensive study of the triterpenoids produced by the stems of Kadsura ananosma, has led to the isolation of eleven new ones, kadnanolactones A-I (1-4,7-11) and kadnanosic acids A(S) and B (6), and six known analogues. Their structures were elucidated mainly by comprehensive spectroscopic analysis, and DFT computational methods were applied to validate the stereochemistry of an epoxide in compound 7. All triterpenoids were evaluated for their cytotoxicity against HL-60, SMMC-7721, A-549, PANC-1, and SK-BR-3 human cancer cells. (C) 2010 Elsevier Ltd. All rights reserved.

The 18-(13-->12 beta)-abeo-lanostene triterpenoid acid, ananosic acid A (1), and the dibenzocyclooctadiene lignan, ananosin A (2), were isolated from the stem bark of Kadsura ananosma. Their structures were elucidated by extensive spectral studies and the structure of 1 was confirmed by single crystal X-ray diffraction analysis.